{"id":"https://openalex.org/W7224880964","doi":"https://doi.org/10.64898/2026.10.29.762880","title":"Comparative Proteomics Reveals Cell-Type-Specific Heterogeneity of Human Neural Cell-Derived Extracellular Vesicles","display_name":"Comparative Proteomics Reveals Cell-Type-Specific Heterogeneity of Human Neural Cell-Derived Extracellular Vesicles","publication_year":2026,"publication_date":"2026-10-30","ids":{"openalex":"https://openalex.org/W7224880964","doi":"https://doi.org/10.64898/2026.10.29.762880"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.29.762880","doi":"https://doi.org/10.64898/2026.10.29.762880","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.29.762880","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/30/2026.10.29.762880.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/30/2026.10.29.762880.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042627183","display_name":"Xinqian Chen","orcid":"https://orcid.org/0000-0002-8558-9250","observed_orcids":["https://orcid.org/0000-0002-8558-9250"]},"institutions":[{"id":"https://openalex.org/I70983195","display_name":"Syracuse University","ror":"https://ror.org/025r5qe02","country_code":"US","type":"education","lineage":["https://openalex.org/I70983195"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xinqian Chen","raw_affiliation_strings":["Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA","Interdisciplinary Neuroscience Program, Syracuse University, Syracuse, NY 13244, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA","institution_ids":["https://openalex.org/I70983195"]},{"raw_affiliation_string":"Interdisciplinary Neuroscience Program, Syracuse University, Syracuse, NY 13244, USA","institution_ids":["https://openalex.org/I70983195"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052345916","display_name":"Chih Hung Lo","orcid":"https://orcid.org/0000-0003-2717-4484","observed_orcids":["https://orcid.org/0000-0003-2717-4484"]},"institutions":[{"id":"https://openalex.org/I70983195","display_name":"Syracuse University","ror":"https://ror.org/025r5qe02","country_code":"US","type":"education","lineage":["https://openalex.org/I70983195"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chih Hung Lo","raw_affiliation_strings":["Department of Biology, Syracuse University, Syracuse, NY 13244, USA","Interdisciplinary Neuroscience Program, Syracuse University, Syracuse, NY 13244, USA"],"raw_orcid":"https://orcid.org/0000-0003-2717-4484","affiliations":[{"raw_affiliation_string":"Department of Biology, Syracuse University, Syracuse, NY 13244, USA","institution_ids":["https://openalex.org/I70983195"]},{"raw_affiliation_string":"Interdisciplinary Neuroscience Program, Syracuse University, Syracuse, NY 13244, USA","institution_ids":["https://openalex.org/I70983195"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018895606","display_name":"Jialiu Zeng","orcid":"https://orcid.org/0000-0001-7802-1432","observed_orcids":["https://orcid.org/0000-0001-7802-1432"]},"institutions":[{"id":"https://openalex.org/I70983195","display_name":"Syracuse University","ror":"https://ror.org/025r5qe02","country_code":"US","type":"education","lineage":["https://openalex.org/I70983195"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jialiu Zeng","raw_affiliation_strings":["Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA","Interdisciplinary Neuroscience Program, Syracuse University, Syracuse, NY 13244, USA"],"raw_orcid":"https://orcid.org/0000-0001-7802-1432","affiliations":[{"raw_affiliation_string":"Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA","institution_ids":["https://openalex.org/I70983195"]},{"raw_affiliation_string":"Interdisciplinary Neuroscience Program, Syracuse University, Syracuse, NY 13244, USA","institution_ids":["https://openalex.org/I70983195"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5018895606"],"corresponding_institution_ids":["https://openalex.org/I70983195"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/extracellular-vesicles","display_name":"extracellular vesicles","score":0.9990000128746033},{"id":"https://openalex.org/keywords/neural-cell-types","display_name":"neural cell types","score":0.6729999780654907},{"id":"https://openalex.org/keywords/sh-sy5y-cells","display_name":"SH-SY5Y cells","score":0.8479999899864197},{"id":"https://openalex.org/keywords/astrocytes","display_name":"astrocytes","score":0.9810000061988831},{"id":"https://openalex.org/keywords/microglia","display_name":"microglia","score":0.9139999747276306},{"id":"https://openalex.org/keywords/neuron-like-cells","display_name":"neuron-like cells","score":0.6710000038146973},{"id":"https://openalex.org/keywords/chromatin-proteins","display_name":"chromatin proteins","score":0.8830000162124634},{"id":"https://openalex.org/keywords/cargo-sorting","display_name":"cargo sorting","score":0.7289999723434448},{"id":"https://openalex.org/keywords/drug-delivery-to-the-brain","display_name":"brain drug delivery","score":0.5099999904632568},{"id":"https://openalex.org/keywords/ev-biomarkers","display_name":"EV biomarkers","score":0.4399999976158142}],"concepts":[{"id":"https://openalex.org/C2908689518","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicle","level":5,"score":0.5981000065803528},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.5734000205993652},{"id":"https://openalex.org/C2992929900","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicles","level":2,"score":0.5527999997138977},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.5415999889373779},{"id":"https://openalex.org/C80311884","wikidata":"https://www.wikidata.org/wiki/Q7268939","display_name":"Quantitative proteomics","level":4,"score":0.5175999999046326},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.491100013256073},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.490200012922287},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.48080000281333923},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.45680001378059387},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.45179998874664307},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.4397999942302704},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40220001339912415},{"id":"https://openalex.org/C2781261824","wikidata":"https://www.wikidata.org/wiki/Q903634","display_name":"Exosome","level":5,"score":0.3799999952316284},{"id":"https://openalex.org/C2778120352","wikidata":"https://www.wikidata.org/wiki/Q3870163","display_name":"Nanoparticle tracking analysis","level":5,"score":0.3612000048160553},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.34929999709129333},{"id":"https://openalex.org/C68289359","wikidata":"https://www.wikidata.org/wiki/Q7502514","display_name":"Shotgun proteomics","level":4,"score":0.3433000147342682},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.328000009059906},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3190999925136566},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.3188999891281128},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.31450000405311584},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.30559998750686646},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.2935999929904938},{"id":"https://openalex.org/C140051345","wikidata":"https://www.wikidata.org/wiki/Q7251501","display_name":"Protein subcellular localization prediction","level":3,"score":0.29089999198913574},{"id":"https://openalex.org/C2780640218","wikidata":"https://www.wikidata.org/wiki/Q8277","display_name":"Multiple sclerosis","level":2,"score":0.2870999872684479},{"id":"https://openalex.org/C73555534","wikidata":"https://www.wikidata.org/wiki/Q622825","display_name":"Cluster analysis","level":2,"score":0.2833999991416931},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.26339998841285706},{"id":"https://openalex.org/C55105296","wikidata":"https://www.wikidata.org/wiki/Q841382","display_name":"Interaction network","level":3,"score":0.2547999918460846},{"id":"https://openalex.org/C57581600","wikidata":"https://www.wikidata.org/wiki/Q2449730","display_name":"Transport protein","level":2,"score":0.25279998779296875}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.29.762880","doi":"https://doi.org/10.64898/2026.10.29.762880","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.29.762880","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/30/2026.10.29.762880.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.29.762880","doi":"https://doi.org/10.64898/2026.10.29.762880","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.29.762880","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/30/2026.10.29.762880.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320309532","display_name":"Syracuse University","ror":"https://ror.org/025r5qe02"},{"id":"https://openalex.org/F4320309644","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08"},{"id":"https://openalex.org/F4320333038","display_name":"State University of New York Upstate Medical University","ror":"https://ror.org/040kfrw16"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7224880964.pdf","grobid_xml":"https://content.openalex.org/works/W7224880964.grobid-xml"},"referenced_works_count":46,"referenced_works":["https://openalex.org/W1594747697","https://openalex.org/W1973012451","https://openalex.org/W2006900120","https://openalex.org/W2011710911","https://openalex.org/W2078424563","https://openalex.org/W2081098333","https://openalex.org/W2081128658","https://openalex.org/W2115462905","https://openalex.org/W2118650749","https://openalex.org/W2125737877","https://openalex.org/W2130410032","https://openalex.org/W2136850043","https://openalex.org/W2144754434","https://openalex.org/W2146512944","https://openalex.org/W2152624886","https://openalex.org/W2159675211","https://openalex.org/W2172557341","https://openalex.org/W2259481044","https://openalex.org/W2511373877","https://openalex.org/W2603189578","https://openalex.org/W2611827321","https://openalex.org/W2933304441","https://openalex.org/W2952615074","https://openalex.org/W2963597305","https://openalex.org/W3014919511","https://openalex.org/W3017163794","https://openalex.org/W3126779750","https://openalex.org/W3168265815","https://openalex.org/W3173849407","https://openalex.org/W3175752499","https://openalex.org/W4205618229","https://openalex.org/W4226051159","https://openalex.org/W4280504805","https://openalex.org/W4300981921","https://openalex.org/W4308034614","https://openalex.org/W4308834893","https://openalex.org/W4313897775","https://openalex.org/W4362586250","https://openalex.org/W4379513113","https://openalex.org/W4391649568","https://openalex.org/W4393995967","https://openalex.org/W4400735087","https://openalex.org/W4407778183","https://openalex.org/W4417303892","https://openalex.org/W4417337131","https://openalex.org/W7160144297"],"related_works":["https://openalex.org/W7160144297","https://openalex.org/W7171977258","https://openalex.org/W4385746709","https://openalex.org/W2917235586","https://openalex.org/W4417303892","https://openalex.org/W7120252548","https://openalex.org/W3176898361","https://openalex.org/W7214009416","https://openalex.org/W7223400515","https://openalex.org/W4385751542"],"abstract_inverted_index":{"Abstract":[0],"Extracellular":[1],"vesicles":[2],"(EVs)":[3],"are":[4],"increasingly":[5],"explored":[6],"as":[7,10],"biomarkers":[8],"and":[9,41,63,95,102,119,124,135,142,147,166,168,174,181,227],"drug":[11],"delivery":[12],"vehicles":[13],"for":[14,48,217,224],"the":[15,35,42,115,192,198],"brain,":[16],"yet":[17],"how":[18],"EV":[19,39,46,86,110,210,222],"cargo":[20,128,162,232],"differs":[21],"among":[22],"neural":[23,214],"cell":[24,215],"types":[25],"at":[26],"baseline":[27,206],"remains":[28],"poorly":[29],"defined.":[30],"This":[31],"gap":[32],"limits":[33],"both":[34],"interpretation":[36],"of":[37,45,172,191,209],"disease-associated":[38,219],"changes":[40],"rational":[43],"choice":[44],"source":[47],"therapeutic":[49,225],"use.":[50],"Here,":[51],"we":[52],"compared":[53],"EVs":[54],"from":[55,212],"human":[56,213],"neuronal-like":[57],"SH-SY5Y":[58],"cells":[59],"(N-EV),":[60],"astrocytes":[61],"(AS-EV),":[62],"HMC3":[64],"microglia":[65],"(MG-EV)":[66],"side":[67,69],"by":[68,90,164,179],"using":[70],"tandem":[71],"mass":[72],"tag":[73],"(TMT)-based":[74],"quantitative":[75],"proteomics,":[76],"with":[77,130,197],"all":[78],"samples":[79],"analyzed":[80],"in":[81,138,145,150],"a":[82,169,205],"single":[83],"multiplexed":[84],"batch.":[85],"identity":[87],"was":[88],"supported":[89],"TEM,":[91],"nanoparticle":[92],"tracking":[93],"analysis,":[94],"canonical":[96,158],"MISEV2023":[97],"markers.":[98],"Size,":[99],"zeta":[100],"potential,":[101],"protein":[103,120],"content":[104],"per":[105],"vesicle":[106],"were":[107,195],"comparable":[108],"across":[109],"types,":[111],"whereas":[112],"N-EV":[113,165],"showed":[114,127],"highest":[116],"particle":[117],"concentration":[118],"yield.":[121],"Enrichment":[122],"analyses":[123],"consensus":[125],"clustering":[126],"consistent":[129,196],"parental-cell":[131],"functions:":[132],"neuronal":[133],"connectivity":[134],"structural":[136],"remodeling":[137],"N-EV,":[139],"extracellular":[140],"support":[141],"cellular":[143],"interaction":[144,189],"AS-EV,":[146],"immune":[148],"surveillance":[149],"MG-EV.":[151,182],"Cross-comparison":[152],"also":[153],"revealed":[154],"patterns":[155],"beyond":[156],"these":[157,202],"roles,":[159],"including":[160],"complement-associated":[161],"shared":[163,178],"MG-EV":[167],"Glial-EV":[170],"signature":[171,187],"chromatin-associated":[173],"epigenetic":[175],"regulatory":[176],"proteins":[177],"AS-EV":[180],"We":[183],"further":[184],"defined":[185],"cell-type-specific":[186],"proteins;":[188],"networks":[190],"larger":[193],"sets":[194],"enrichment":[199],"results.":[200],"Overall,":[201],"data":[203],"provide":[204],"comparative":[207],"reference":[208],"proteomes":[211],"models":[216],"interpreting":[218],"alterations,":[220],"selecting":[221],"sources":[223],"use,":[226],"generating":[228],"hypotheses":[229],"on":[230],"selective":[231],"sorting.":[233]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-10-31T00:00:00"}
