{"id":"https://openalex.org/W7224857117","doi":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","title":"Development of an Efficient in vitro Propagation Protocol and ISSR Based Clonal Fidelity Assessment in Bambusa vulgaris","display_name":"Development of an Efficient in vitro Propagation Protocol and ISSR Based Clonal Fidelity Assessment in Bambusa vulgaris","publication_year":2026,"publication_date":"2026-10-30","ids":{"openalex":"https://openalex.org/W7224857117","doi":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292"},"language":"en","primary_location":{"id":"doi:10.56557/pcbmb/2026/v27i11-1211292","doi":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","is_oa":true,"landing_page_url":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","pdf_url":null,"source":{"id":"https://openalex.org/S4306522609","display_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","issn_l":"0972-2025","issn":["0972-2025"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076625052","display_name":"Pragati Baghel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20632288","display_name":"Indira Gandhi Agricultural University","ror":"https://ror.org/00mcwq335","country_code":"IN","type":"education","lineage":["https://openalex.org/I20632288"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Pragati Baghel","raw_affiliation_strings":["Department of Molecular Biology and Biotechnology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Biotechnology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh, India","institution_ids":["https://openalex.org/I20632288"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088914937","display_name":"Zenu Jha","orcid":"https://orcid.org/0000-0003-4029-7497","observed_orcids":["https://orcid.org/0000-0003-4029-7497"]},"institutions":[{"id":"https://openalex.org/I20632288","display_name":"Indira Gandhi Agricultural University","ror":"https://ror.org/00mcwq335","country_code":"IN","type":"education","lineage":["https://openalex.org/I20632288"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Zenu Jha","raw_affiliation_strings":["Department of Molecular Biology and Biotechnology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Biotechnology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh, India","institution_ids":["https://openalex.org/I20632288"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115572738","display_name":"Akanksha Lakra","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20632288","display_name":"Indira Gandhi Agricultural University","ror":"https://ror.org/00mcwq335","country_code":"IN","type":"education","lineage":["https://openalex.org/I20632288"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Akanksha Lakra","raw_affiliation_strings":["Department of Molecular Biology and Biotechnology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Biotechnology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh, India","institution_ids":["https://openalex.org/I20632288"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5115420904","display_name":"Shikha Minj","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shikha Minj","raw_affiliation_strings":["Naveen Govt. College, Raghunathnagar, Balrampur-Ramanujganj, Chhattisgarh, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Naveen Govt. College, Raghunathnagar, Balrampur-Ramanujganj, Chhattisgarh, India","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.74620864,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"27","issue":"11-12","first_page":"50","last_page":"62"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12665","display_name":"Bamboo properties and applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bambusa-vulgaris","display_name":"Bambusa vulgaris","score":1.0},{"id":"https://openalex.org/keywords/bamboo","display_name":"bamboo","score":0.8320000171661377},{"id":"https://openalex.org/keywords/micropropagation","display_name":"micropropagation","score":0.9539999961853027},{"id":"https://openalex.org/keywords/issr-markers","display_name":"ISSR markers","score":0.7860000133514404},{"id":"https://openalex.org/keywords/clonal-fidelity","display_name":"clonal fidelity","score":0.9610000252723694},{"id":"https://openalex.org/keywords/genetic-homogeneity","display_name":"genetic homogeneity","score":0.7250000238418579},{"id":"https://openalex.org/keywords/shoot-multiplication","display_name":"shoot multiplication","score":0.7879999876022339},{"id":"https://openalex.org/keywords/rooting","display_name":"rooting","score":0.6819999814033508},{"id":"https://openalex.org/keywords/acclimatization","display_name":"acclimatization","score":0.9079999923706055},{"id":"https://openalex.org/keywords/plant-growth-regulators","display_name":"plant growth regulators","score":0.7950000166893005},{"id":"https://openalex.org/keywords/bap","display_name":"BAP","score":0.8429999947547913},{"id":"https://openalex.org/keywords/kinetin","display_name":"kinetin","score":0.9480000138282776},{"id":"https://openalex.org/keywords/naa","display_name":"NAA","score":0.9210000038146973},{"id":"https://openalex.org/keywords/iba","display_name":"IBA","score":0.9369999766349792},{"id":"https://openalex.org/keywords/surface-sterilization","display_name":"surface sterilization","score":0.6919999718666077},{"id":"https://openalex.org/keywords/germplasm-conservation","display_name":"germplasm conservation","score":0.8489999771118164},{"id":"https://openalex.org/keywords/tissue-culture","display_name":"tissue culture","score":0.5080000162124634}],"concepts":[{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.7928000092506409},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7626000046730042},{"id":"https://openalex.org/C2776499888","wikidata":"https://www.wikidata.org/wiki/Q2251215","display_name":"Kinetin","level":4,"score":0.7465999722480774},{"id":"https://openalex.org/C2780822416","wikidata":"https://www.wikidata.org/wiki/Q1934105","display_name":"Micropropagation","level":4,"score":0.7226999998092651},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.7125999927520752},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5213000178337097},{"id":"https://openalex.org/C119406331","wikidata":"https://www.wikidata.org/wiki/Q419763","display_name":"Acclimatization","level":2,"score":0.48750001192092896},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.4546999931335449},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.4307999908924103},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.4083000123500824},{"id":"https://openalex.org/C2778057067","wikidata":"https://www.wikidata.org/wiki/Q1479412","display_name":"Plant propagation","level":2,"score":0.4047999978065491},{"id":"https://openalex.org/C74103781","wikidata":"https://www.wikidata.org/wiki/Q199182","display_name":"Ornamental plant","level":2,"score":0.3093999922275543},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.30559998750686646},{"id":"https://openalex.org/C2778704284","wikidata":"https://www.wikidata.org/wiki/Q670887","display_name":"Bamboo","level":2,"score":0.28139999508857727},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.27970001101493835},{"id":"https://openalex.org/C197321923","wikidata":"https://www.wikidata.org/wiki/Q4886","display_name":"Cultivar","level":2,"score":0.27810001373291016},{"id":"https://openalex.org/C2776459999","wikidata":"https://www.wikidata.org/wiki/Q2119376","display_name":"Fidelity","level":2,"score":0.2653000056743622}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.56557/pcbmb/2026/v27i11-1211292","doi":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","is_oa":true,"landing_page_url":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","pdf_url":null,"source":{"id":"https://openalex.org/S4306522609","display_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","issn_l":"0972-2025","issn":["0972-2025"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","raw_type":"journal-article"},{"id":"pmh:oai:ikprress.org:article/11292","doi":null,"is_oa":true,"landing_page_url":"https://ikprress.org/index.php/PCBMB/article/view/11292","pdf_url":null,"source":{"id":"https://openalex.org/S4306522609","display_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","issn_l":"0972-2025","issn":["0972-2025"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"0972-2025","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:HAL:hal-05846318v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.science/hal-05846318","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Plant Cell Biotechnology and Molecular Biology, 2026, 27 (11-12), pp.50-62","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.56557/pcbmb/2026/v27i11-1211292","doi":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","is_oa":true,"landing_page_url":"https://doi.org/10.56557/pcbmb/2026/v27i11-1211292","pdf_url":null,"source":{"id":"https://openalex.org/S4306522609","display_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","issn_l":"0972-2025","issn":["0972-2025"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2973082671","https://openalex.org/W2315575029","https://openalex.org/W4366779647","https://openalex.org/W4251416053","https://openalex.org/W1975689594","https://openalex.org/W1511987385","https://openalex.org/W4414917368","https://openalex.org/W2623526203","https://openalex.org/W4405887638","https://openalex.org/W4416253136"],"abstract_inverted_index":{"Bambusa":[0,143,305],"vulgaris":[1,144],"is":[2,32,77],"an":[3],"economically":[4],"important":[5],"bamboo":[6,25],"species":[7],"valued":[8],"for":[9,22,90,106,113,124,166,294],"its":[10],"rapid":[11],"growth":[12,164],"and":[13,18,40,59,71,127,148,152,160,171,188,219,235,270,297],"diverse":[14],"industrial,":[15],"environmental,":[16],"biotechnological":[17],"ornamental":[19],"applications.":[20],"However,":[21],"this":[23],"multipurpose":[24],"species,":[26],"large-scale":[27],"propagation":[28,42,51],"through":[29],"conventional":[30],"methods":[31],"constrained":[33],"by":[34],"irregular":[35],"flowering,":[36],"poor":[37],"seed":[38],"availability":[39],"low":[41],"efficiency.":[43],"Although":[44],"several":[45],"studies":[46],"have":[47],"reported":[48],"in":[49,107,240],"vitro":[50,108],"protocols,":[52],"there":[53],"remains":[54],"a":[55,120,291],"lack":[56],"of":[57,83,133,142,162,263,284,300,304],"standardised":[58],"reproducible":[60,271],"media":[61,105,111],"compositions":[62],"that":[63,261],"consistently":[64],"achieve":[65],"high":[66],"establishment,":[67,109],"shoot":[68,115,167,191,207],"multiplication,":[69,116,169],"rooting":[70,170],"successful":[72],"acclimatization.":[73],"Furthermore,":[74],"limited":[75],"information":[76],"available":[78],"regarding":[79],"the":[80,88,103,130,205,264,277,281,285,298],"genetic":[81,131,282],"fidelity":[82,255],"tissue":[84],"culture-derived":[85],"plants,":[86],"highlighting":[87],"need":[89],"molecular":[91,138],"validation":[92],"to":[93,101,118,128,276],"ensure":[94],"clonal":[95],"uniformity.":[96],"The":[97,173,243,287],"present":[98],"study":[99],"aims":[100],"standardise":[102],"culture":[104],"optimise":[110],"composition":[112],"efficient":[114],"rooting,":[117],"develop":[119],"reliable":[121,292],"hardening":[122,252],"protocol":[123,289],"regenerated":[125,134],"plantlets":[126],"assess":[129],"uniformity":[132,283],"plants":[135],"using":[136,180,257],"ISSR":[137,258],"markers.":[139],"Nodal":[140],"explants":[141],"were":[145],"surface":[146],"sterilized":[147],"cultured":[149],"on":[150,196,211,227],"Murashige":[151],"Skoog":[153],"(MS)":[154],"medium":[155,198,214,229],"supplemented":[156,199,230],"with":[157,200,231],"different":[158],"concentrations":[159],"combinations":[161],"plant":[163,302],"regulators":[165],"induction,":[168],"hardening.":[172],"highest":[174,206,244],"explant":[175],"survivability":[176],"(84%)":[177],"was":[178,194,209,225,248],"achieved":[179,249],"70%":[181],"ethanol,":[182],"Tween-20,":[183],"1%":[184],"Bavistin,":[185],"0.1%":[186],"HgCl\u2082":[187],"antibiotics.":[189],"Maximum":[190],"initiation":[192],"(88%)":[193],"obtained":[195],"MS":[197,213,228],"BAP":[201,216],"(2.5":[202],"mg/L-1),":[203,238],"while":[204],"multiplication":[208],"recorded":[210],"solid":[212],"containing":[215],"(2.0":[217],"mg/L-1)":[218,234],"kinetin":[220],"(0.5":[221,237],"mg/L-1).":[222],"Root":[223],"induction":[224],"optimized":[226],"NAA":[232],"(3.0":[233],"IBA":[236],"resulting":[239],"80%":[241],"rooting.":[242],"acclimatization":[245],"success":[246],"(80%)":[247],"under":[250],"H2":[251],"conditions.":[253],"Genetic":[254],"analysis":[256],"markers":[259],"showed":[260],"8":[262],"16":[265],"primers":[266],"screened":[267],"produced":[268],"clear":[269],"monomorphic":[272],"banding":[273],"patterns":[274],"identical":[275],"mother":[278],"plant,":[279],"confirming":[280],"regenerants.":[286],"standardized":[288],"provides":[290],"system":[293],"germplasm":[295],"conservation":[296],"production":[299],"quality":[301],"material":[303],"vulgaris.":[306]},"counts_by_year":[],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2026-10-31T00:00:00"}