{"id":"https://openalex.org/W7224790078","doi":"https://doi.org/10.1094/phyto-07-26-0221-r","title":"A High-Sensitivity RPA/Cas12a Assay for Rapid Detection of the Select Agent Ralstonia solanacearum R3b2","display_name":"A High-Sensitivity RPA/Cas12a Assay for Rapid Detection of the Select Agent Ralstonia solanacearum R3b2","publication_year":2026,"publication_date":"2026-10-30","ids":{"openalex":"https://openalex.org/W7224790078","doi":"https://doi.org/10.1094/phyto-07-26-0221-r","pmid":"https://pubmed.ncbi.nlm.nih.gov/42961866"},"language":"en","primary_location":{"id":"doi:10.1094/phyto-07-26-0221-r","doi":"https://doi.org/10.1094/phyto-07-26-0221-r","is_oa":false,"landing_page_url":"https://doi.org/10.1094/phyto-07-26-0221-r","pdf_url":null,"source":{"id":"https://openalex.org/S85201822","display_name":"Phytopathology","issn_l":"0031-949X","issn":["0031-949X","1943-7684"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Phytopathology\u00ae","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5160216905","display_name":"Sydney Ostlund","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sydney Ostlund","raw_affiliation_strings":["The Pennsylvania State University, Plant Pathology and Environmental Microbiology, University Park, Pennsylvania, United States, ;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Pennsylvania State University, Plant Pathology and Environmental Microbiology, University Park, Pennsylvania, United States, ;","institution_ids":["https://openalex.org/I130769515"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160214879","display_name":"Hsu Yang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hsu Yang","raw_affiliation_strings":["The Pennsylvania State University, Plant Pathology and Environmental Microbiology, University Park, Pennsylvania, United States, ;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Pennsylvania State University, Plant Pathology and Environmental Microbiology, University Park, Pennsylvania, United States, ;","institution_ids":["https://openalex.org/I130769515"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007879381","display_name":"Eric A. Newberry","orcid":"https://orcid.org/0000-0002-3171-0801","observed_orcids":["https://orcid.org/0000-0002-3171-0801"]},"institutions":[{"id":"https://openalex.org/I1288380561","display_name":"Animal and Plant Health Inspection Service","ror":"https://ror.org/0599wfz09","country_code":"US","type":"government","lineage":["https://openalex.org/I1288380561","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I4210125976","display_name":"Beltsville Agricultural Research Center","ror":"https://ror.org/03b08sh51","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210125976","https://openalex.org/I4210138094"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric Newberry","raw_affiliation_strings":["USDA-APHIS PPQ, Bldg 580, BARC-East, 9901 Powder Mill Rd, Laurel, Maryland, United States, 20708;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USDA-APHIS PPQ, Bldg 580, BARC-East, 9901 Powder Mill Rd, Laurel, Maryland, United States, 20708;","institution_ids":["https://openalex.org/I1288380561","https://openalex.org/I4210125976"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002854706","display_name":"Stefano Costanzo","orcid":"https://orcid.org/0000-0003-0330-3827","observed_orcids":["https://orcid.org/0000-0003-0330-3827"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I4210125976","display_name":"Beltsville Agricultural Research Center","ror":"https://ror.org/03b08sh51","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210125976","https://openalex.org/I4210138094"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stefano Costanzo","raw_affiliation_strings":["USDA Agricultural Research Service, Beltsville, Maryland, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USDA Agricultural Research Service, Beltsville, Maryland, United States;","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210125976"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022551274","display_name":"Ye Yang","orcid":"https://orcid.org/0000-0002-7576-3067","observed_orcids":["https://orcid.org/0000-0002-7576-3067"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yinong Yang","raw_affiliation_strings":["The Pennsylvania State University, Plant Pathology and Environmental Microbiology, 405C Life Sciences Bldg, University Park, Pennsylvania, United States, 16803, , ;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Pennsylvania State University, Plant Pathology and Environmental Microbiology, 405C Life Sciences Bldg, University Park, Pennsylvania, United States, 16803, , ;","institution_ids":["https://openalex.org/I130769515"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.84449985,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.888700008392334,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.888700008392334,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.09300000220537186,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.016200000420212746,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacterial-wilt","display_name":"bacterial wilt","score":0.9440000057220459},{"id":"https://openalex.org/keywords/crispr-cas12a","display_name":"CRISPR/Cas12a","score":0.8880000114440918},{"id":"https://openalex.org/keywords/crispr-based-diagnostics","display_name":"CRISPR-based diagnostics","score":0.7910000085830688},{"id":"https://openalex.org/keywords/point-of-care-testing","display_name":"point-of-care testing","score":0.9620000123977661},{"id":"https://openalex.org/keywords/fluorescent-reporter","display_name":"fluorescent reporter","score":0.5109999775886536},{"id":"https://openalex.org/keywords/pathogen-surveillance","display_name":"pathogen surveillance","score":0.6690000295639038},{"id":"https://openalex.org/keywords/plant-pathogen-detection","display_name":"plant pathogen detection","score":0.5730000138282776},{"id":"https://openalex.org/keywords/soil-bacteria","display_name":"soil bacteria","score":0.4909999966621399}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8644000291824341},{"id":"https://openalex.org/C2780675535","wikidata":"https://www.wikidata.org/wiki/Q2710840","display_name":"Ralstonia solanacearum","level":3,"score":0.861299991607666},{"id":"https://openalex.org/C60635243","wikidata":"https://www.wikidata.org/wiki/Q6675780","display_name":"Loop-mediated isothermal amplification","level":3,"score":0.7918000221252441},{"id":"https://openalex.org/C2779002231","wikidata":"https://www.wikidata.org/wiki/Q4840003","display_name":"Bacterial wilt","level":2,"score":0.7440999746322632},{"id":"https://openalex.org/C139713532","wikidata":"https://www.wikidata.org/wiki/Q17077657","display_name":"Recombinase Polymerase Amplification","level":4,"score":0.5769000053405762},{"id":"https://openalex.org/C2778967756","wikidata":"https://www.wikidata.org/wiki/Q1972080","display_name":"Biovar","level":3,"score":0.49149999022483826},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.48590001463890076},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.46540001034736633},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4047999978065491},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3815999925136566},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.3440000116825104},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3422999978065491},{"id":"https://openalex.org/C143660080","wikidata":"https://www.wikidata.org/wiki/Q7684883","display_name":"TaqMan","level":4,"score":0.3391000032424927},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.3199999928474426},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3160000145435333},{"id":"https://openalex.org/C2780968160","wikidata":"https://www.wikidata.org/wiki/Q423692","display_name":"SYBR Green I","level":4,"score":0.31540000438690186},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.31220000982284546},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.3091000020503998},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.2904999852180481},{"id":"https://openalex.org/C2780734198","wikidata":"https://www.wikidata.org/wiki/Q948017","display_name":"Ralstonia","level":3,"score":0.28690001368522644},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.2669000029563904},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.259799987077713},{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.2547999918460846},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.25360000133514404}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1094/phyto-07-26-0221-r","doi":"https://doi.org/10.1094/phyto-07-26-0221-r","is_oa":false,"landing_page_url":"https://doi.org/10.1094/phyto-07-26-0221-r","pdf_url":null,"source":{"id":"https://openalex.org/S85201822","display_name":"Phytopathology","issn_l":"0031-949X","issn":["0031-949X","1943-7684"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Phytopathology\u00ae","raw_type":"journal-article"},{"id":"pmid:42961866","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42961866","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Phytopathology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.6735000014305115}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2061001127","https://openalex.org/W1683482550","https://openalex.org/W3177547139","https://openalex.org/W7221344657","https://openalex.org/W1993649039","https://openalex.org/W4319322269","https://openalex.org/W2256267289","https://openalex.org/W4387879833","https://openalex.org/W2024224397","https://openalex.org/W4377205996"],"abstract_inverted_index":{"Ralstonia":[0],"solanacearum":[1,150],"is":[2,23,33,52],"a":[3,24,55,130,154,200],"globally":[4],"distributed,":[5],"soil-borne":[6],"species":[7],"complex":[8,31],"that":[9,32],"causes":[10],"bacterial":[11],"wilt":[12],"in":[13,40,94,232],"many":[14],"economically":[15],"important":[16],"crops.":[17],"Race":[18],"3":[19],"biovar":[20],"2":[21],"(R3b2)":[22],"cold-tolerant,":[25],"highly":[26],"aggressive":[27],"subgroup":[28],"within":[29],"this":[30,77,223],"responsible":[34],"for":[35,76,90,106,120],"an":[36,113,159],"estimated":[37],"$1":[38],"billion":[39],"annual":[41],"global":[42],"losses.":[43],"Because":[44],"R3b2":[45,142,245],"remains":[46],"exotic":[47],"to":[48,60,64],"North":[49],"America,":[50],"it":[51],"classified":[53],"as":[54,103,184,186,210],"USDA":[56],"Select":[57],"Agent":[58],"due":[59],"its":[61],"high":[62],"potential":[63],"devastate":[65],"U.S.":[66],"solanaceous":[67],"crops":[68],"and":[69,85,123,146,158,169,214,221],"ornamental":[70],"industries.":[71],"However,":[72],"current":[73,226],"diagnostic":[74,227],"methods":[75],"pathogen":[78],"involve":[79],"trade-offs":[80],"among":[81],"cost,":[82],"sensitivity,":[83],"specificity,":[84],"ease":[86],"of":[87,126,153,176,244],"use,":[88],"particularly":[89],"point-of-care":[91],"(POC)":[92],"detection":[93,98,125,171],"field":[95],"settings.":[96],"CRISPR-based":[97],"platforms":[99],"have":[100],"recently":[101],"emerged":[102],"promising":[104],"tools":[105],"high-sensitivity":[107],"POC":[108,193],"diagnostics.":[109],"Here":[110],"we":[111],"developed":[112],"isothermal":[114],"recombinase":[115],"polymerase":[116],"amplification":[117],"(RPA)/Cas12a":[118],"assay":[119,139,182,224],"specific,":[121],"sensitive":[122],"rapid":[124],"R3b2.":[127],"By":[128,217],"targeting":[129],"genomic":[131],"region":[132],"associated":[133],"with":[134,205],"the":[135,138,181,195,242,247],"cool-virulence":[136],"phenotype,":[137],"reliably":[140],"differentiates":[141],"from":[143],"plant":[144],"hosts":[145],"other":[147],"diverse":[148],"R.":[149],"strains.":[151],"Incorporation":[152],"dual-guide":[155],"RNA":[156],"system":[157,196],"8-mer":[160],"single-stranded":[161],"DNA":[162,179],"(ssDNA)":[163],"fluorescent":[164],"reporter":[165],"further":[166],"improved":[167],"sensitivity":[168,220],"reduced":[170],"time.":[172],"Using":[173],"dilution":[174],"series":[175],"R3b2-spiked":[177],"Pelargonium":[178],"extracts,":[180],"detected":[183],"few":[185],"4":[187],"CFU":[188],"per":[189],"reaction.":[190],"To":[191],"facilitate":[192],"applications,":[194],"was":[197],"adapted":[198],"into":[199,246],"one-pot,":[201],"contamination-resistan\u00act":[202],"format":[203],"compatible":[204],"portable":[206],"fluorescence":[207],"readers":[208],"such":[209],"Genie":[211],"III":[212],"device":[213],"P51":[215],"viewer.":[216],"improving":[218],"both":[219],"portability,":[222],"enhances":[225],"capabilities":[228],"and,":[229],"pending":[230],"validation":[231],"real-world":[233],"conditions,":[234],"supports":[235],"early":[236],"surveillance":[237],"efforts":[238],"aimed":[239],"at":[240],"preventing":[241],"introduction":[243],"United":[248],"States.":[249]},"counts_by_year":[],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2026-10-31T00:00:00"}
