{"id":"https://openalex.org/W7224743009","doi":"https://doi.org/10.21203/rs.3.rs-11245916/v1","title":"Effects of Nano-Hydroxyapatite-Coated Platelet-Rich Fibrin on In Vitro Wound Healing of Gingiva-Derived Mesenchymal Stem Cells","display_name":"Effects of Nano-Hydroxyapatite-Coated Platelet-Rich Fibrin on In Vitro Wound Healing of Gingiva-Derived Mesenchymal Stem Cells","publication_year":2026,"publication_date":"2026-10-30","ids":{"openalex":"https://openalex.org/W7224743009","doi":"https://doi.org/10.21203/rs.3.rs-11245916/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11245916/v1","doi":"https://doi.org/10.21203/rs.3.rs-11245916/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11245916/v1","pdf_url":"https://www.researchsquare.com/article/rs-11245916/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-11245916/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5139384816","display_name":"\u0130zzet Melih G\u00fcrkan","orcid":"https://orcid.org/0000-0002-7267-4798","observed_orcids":["https://orcid.org/0000-0002-7267-4798"]},"institutions":[{"id":"https://openalex.org/I51209816","display_name":"Eski\u015fehir Osmangazi University","ror":"https://ror.org/01dzjez04","country_code":"TR","type":"education","lineage":["https://openalex.org/I51209816"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"\u0130zzet Melih G\u00fcrkan","raw_affiliation_strings":["Eski\u015fehir Osmangazi University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Eski\u015fehir Osmangazi University","institution_ids":["https://openalex.org/I51209816"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005199534","display_name":"Se\u00e7il \u00c7ALI\u015eKAN","orcid":"https://orcid.org/0000-0002-8099-584X","observed_orcids":["https://orcid.org/0000-0002-8099-584X"]},"institutions":[{"id":"https://openalex.org/I51209816","display_name":"Eski\u015fehir Osmangazi University","ror":"https://ror.org/01dzjez04","country_code":"TR","type":"education","lineage":["https://openalex.org/I51209816"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Se\u00e7il \u00c7al\u0131\u015fkan","raw_affiliation_strings":["Eski\u015fehir Osmangazi University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Eski\u015fehir Osmangazi University","institution_ids":["https://openalex.org/I51209816"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5139332279","display_name":"Sibel \u00d6zdemir","orcid":"https://orcid.org/0009-0003-4671-0964","observed_orcids":["https://orcid.org/0009-0003-4671-0964"]},"institutions":[{"id":"https://openalex.org/I4210113509","display_name":"Yunus Emre Hastanesi","ror":"https://ror.org/02671ys76","country_code":"TR","type":"healthcare","lineage":["https://openalex.org/I4210113509"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Sibel \u00d6zdemir","raw_affiliation_strings":["Yunus Emre State Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yunus Emre State Hospital","institution_ids":["https://openalex.org/I4210113509"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102020896","display_name":"Hakan \u00d6zdemir","orcid":"https://orcid.org/0000-0001-9550-1999","observed_orcids":["https://orcid.org/0000-0001-9550-1999"]},"institutions":[{"id":"https://openalex.org/I51209816","display_name":"Eski\u015fehir Osmangazi University","ror":"https://ror.org/01dzjez04","country_code":"TR","type":"education","lineage":["https://openalex.org/I51209816"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Hakan \u00d6zdemir","raw_affiliation_strings":["Eski\u015fehir Osmangazi University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Eski\u015fehir Osmangazi University","institution_ids":["https://openalex.org/I51209816"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11570","display_name":"Periodontal Regeneration and Treatments","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11570","display_name":"Periodontal Regeneration and Treatments","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10771","display_name":"Wound Healing and Treatments","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2742","display_name":"Rehabilitation"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10059","display_name":"Bone Tissue Engineering Materials","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nano-hydroxyapatite","display_name":"hydroxyapatite nanoparticles","score":0.9430000185966492},{"id":"https://openalex.org/keywords/platelet-rich-fibrin","display_name":"platelet-rich fibrin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/wound-healing","display_name":"wound healing","score":0.8320000171661377},{"id":"https://openalex.org/keywords/conditioned-medium","display_name":"conditioned medium","score":0.8510000109672546},{"id":"https://openalex.org/keywords/scratch-assay","display_name":"scratch assay","score":0.8180000185966492},{"id":"https://openalex.org/keywords/bioactive-scaffolds","display_name":"bioactive scaffolds","score":0.42399999499320984},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.3490000069141388},{"id":"https://openalex.org/keywords/cell-migration","display_name":"cell migration","score":0.421999990940094},{"id":"https://openalex.org/keywords/cell-adhesion","display_name":"cell adhesion","score":0.5960000157356262},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.3499999940395355},{"id":"https://openalex.org/keywords/cell-differentiation","display_name":"cell differentiation","score":0.3720000088214874},{"id":"https://openalex.org/keywords/cellular-senescence","display_name":"cellular senescence","score":0.42500001192092896}],"concepts":[{"id":"https://openalex.org/C2780269544","wikidata":"https://www.wikidata.org/wiki/Q1509074","display_name":"Wound healing","level":2,"score":0.8101999759674072},{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.7268999814987183},{"id":"https://openalex.org/C54173615","wikidata":"https://www.wikidata.org/wiki/Q27205","display_name":"Fibrin","level":2,"score":0.7257000207901001},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.6050999760627747},{"id":"https://openalex.org/C3018023364","wikidata":"https://www.wikidata.org/wiki/Q425265","display_name":"Significant difference","level":2,"score":0.5884000062942505},{"id":"https://openalex.org/C2993873987","wikidata":"https://www.wikidata.org/wiki/Q184753","display_name":"Wound closure","level":3,"score":0.5479999780654907},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4713999927043915},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4302999973297119},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.4196000099182129},{"id":"https://openalex.org/C2777560349","wikidata":"https://www.wikidata.org/wiki/Q18388934","display_name":"Platelet-rich fibrin","level":3,"score":0.4147000014781952},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4009999930858612},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3734000027179718},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.33970001339912415},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.33070001006126404},{"id":"https://openalex.org/C10882517","wikidata":"https://www.wikidata.org/wiki/Q15268054","display_name":"Cell therapy","level":3,"score":0.2705000042915344},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.26510000228881836},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2612999975681305},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.25360000133514404},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.2524000108242035}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-11245916/v1","doi":"https://doi.org/10.21203/rs.3.rs-11245916/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11245916/v1","pdf_url":"https://www.researchsquare.com/article/rs-11245916/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11245916/v1","doi":"https://doi.org/10.21203/rs.3.rs-11245916/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11245916/v1","pdf_url":"https://www.researchsquare.com/article/rs-11245916/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.9763000011444092,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7224743009.pdf","grobid_xml":"https://content.openalex.org/works/W7224743009.grobid-xml"},"referenced_works_count":20,"referenced_works":["https://openalex.org/W2050454842","https://openalex.org/W2054831350","https://openalex.org/W2085293124","https://openalex.org/W2100592123","https://openalex.org/W2102726602","https://openalex.org/W2127903487","https://openalex.org/W2252857368","https://openalex.org/W2553198538","https://openalex.org/W2609539491","https://openalex.org/W2803399583","https://openalex.org/W2991067430","https://openalex.org/W3017275732","https://openalex.org/W3154203597","https://openalex.org/W3175938459","https://openalex.org/W4224227781","https://openalex.org/W4386529476","https://openalex.org/W4401827894","https://openalex.org/W4402749549","https://openalex.org/W4404117669","https://openalex.org/W7165891071"],"related_works":["https://openalex.org/W7165891071","https://openalex.org/W4389279427","https://openalex.org/W4411725197","https://openalex.org/W2883686556","https://openalex.org/W2958221174","https://openalex.org/W7166182668","https://openalex.org/W2282855489","https://openalex.org/W7000008031","https://openalex.org/W4391214035","https://openalex.org/W2222867677"],"abstract_inverted_index":{"Abstract":[0],"Objectives:":[1],"Platelet-rich":[2],"fibrin":[3,30],"(PRF)":[4],"contains":[5],"bioactive":[6],"mediators":[7],"that":[8,346],"may":[9,349,373,380],"support":[10],"cellular":[11],"events":[12],"involved":[13],"in":[14,49,99,198,264,272,361,392],"wound":[15,45,117,194,239,260,301,325,377,396],"healing.":[16,397],"This":[17],"study":[18],"compared":[19,244],"the":[20,112,132,199,236,265,273,291,330,351,387],"effects":[21],"of":[22,94,290,355,371,390],"conditioned":[23,85,114,160,206,295,337],"media":[24,86,296,322],"derived":[25],"from":[26,60,64],"leukocyte-":[27],"and":[28,35,54,68,75,84,103,127,136,201,254,271,293,313,360,379,394],"platelet-rich":[29],"(L-PRF),":[31],"nano-hydroxyapatite-modified":[32],"PRF":[33,37,372,391],"(HA-PRF),":[34],"titanium-prepared":[36],"(T-PRF)":[38],"on":[39],"gingiva-derived":[40],"mesenchymal":[41],"stem":[42],"cell":[43],"(GMSC)":[44],"closure":[46,195,261,284,378],"using":[47,145],"an":[48],"vitro":[50],"scratch":[51],"assay.":[52],"Materials":[53],"Methods":[55],":":[56,157,320,368],"GMSCs":[57,96],"were":[58,78,87,97,119,139,167],"isolated":[59],"gingival":[61],"tissue":[62],"obtained":[63],"a":[65,106,180,221,382],"healthy":[66],"donor":[67],"characterized":[69],"before":[70],"experimental":[71],"use.":[72],"L-PRF,":[73,134],"HA-PRF,":[74,135],"T-PRF":[76,137,250],"membranes":[77],"prepared":[79],"under":[80],"identical":[81],"centrifugation":[82],"conditions,":[83],"collected":[88,339],"after":[89,340],"1":[90],"or":[91,172],"3":[92,341],"days":[93],"incubation.":[95],"seeded":[98],"24-well":[100],"plates,":[101],"serum-starved,":[102],"subjected":[104],"to":[105,111],"linear":[107],"scratch.":[108],"Following":[109],"exposure":[110],"respective":[113],"media,":[115,161,207],"residual":[116,238,300],"areas":[118,302],"evaluated":[120],"at":[121,141,169,186,214,225,307],"0,":[122],"6,":[123,170],"12,":[124,171],"24,":[125],"30,":[126],"36":[128,286],"h.":[129,287],"Comparisons":[130],"among":[131,165],"control,":[133],"groups":[138,166,281],"performed":[140],"each":[142],"observation":[143],"time":[144],"one-way":[146],"ANOVA,":[147],"followed":[148],"by":[149,268,276,285],"Tukey\u2019s":[150],"post":[151],"hoc":[152],"test":[153],"where":[154],"appropriate.":[155],"Results":[156],"For":[158,204],"1-day":[159],"no":[162,208],"significant":[163,181,209,222],"differences":[164],"observed":[168,213],"24":[173,269],"h":[174,188,216,227,270,309,315],"(all":[175],"p":[176],">":[177],"0.05),":[178],"whereas":[179,220],"between-group":[182,210],"difference":[183,211,223],"was":[184,212,262],"detected":[185],"30":[187,277],"(p":[189,217,228,310,316],"=":[190,218,311,317],"0.005),":[191],"when":[192],"complete":[193,283],"had":[196],"occurred":[197],"L-PRF":[200,246,274],"HA-PRF":[202,234,266,328],"groups.":[203],"3-day":[205,294,304],"6":[215,308],"0.209),":[219],"emerged":[224],"12":[226,232,314],"<":[229],"0.001).":[230],"At":[231],"h,":[233,278],"showed":[235,297],"smallest":[237],"area":[240],"(33.93":[241],"\u00b1":[242,248,252,257],"7.96%),":[243],"with":[245,303,327,336],"(57.71":[247],"9.46%),":[249],"(69.44":[251],"9.25%),":[253],"control":[255],"(80.91":[256],"5.33%).":[258],"Complete":[259],"achieved":[263],"group":[267,275],"while":[279],"all":[280],"reached":[282],"Direct":[288],"comparison":[289],"1-":[292],"significantly":[298],"smaller":[299],"HA-PRF-conditioned":[305],"medium":[306,338],"0.024)":[312],"0.011).":[318],"Conclusions":[319],"PRF-conditioned":[321],"supported":[323],"GMSC":[324],"closure,":[326],"showing":[329],"most":[331],"favorable":[332],"early":[333,352,375],"response,":[334],"particularly":[335],"days.":[342],"These":[343],"findings":[344],"suggest":[345],"nano-hydroxyapatite":[347],"modification":[348,370],"enhance":[350,374],"biological":[353],"activity":[354],"PRF;":[356],"however,":[357],"further":[358],"mechanistic":[359],"vivo":[362],"studies":[363],"are":[364],"required.":[365],"Clinical":[366],"Relevance":[367],"Nano-hydroxyapatite":[369],"GMSC-mediated":[376],"represent":[381],"promising":[383],"approach":[384],"for":[385],"improving":[386],"regenerative":[388],"potential":[389],"oral":[393],"periodontal":[395]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-10-31T00:00:00"}