{"id":"https://openalex.org/W7224576108","doi":"https://doi.org/10.64898/2026.10.28.762254","title":"Impact of HIV-1 infection on the microglia transcriptome: induction of microRNA-23b leads to G3BP2-mediated stress granules assembly disruption","display_name":"Impact of HIV-1 infection on the microglia transcriptome: induction of microRNA-23b leads to G3BP2-mediated stress granules assembly disruption","publication_year":2026,"publication_date":"2026-10-29","ids":{"openalex":"https://openalex.org/W7224576108","doi":"https://doi.org/10.64898/2026.10.28.762254"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.28.762254","doi":"https://doi.org/10.64898/2026.10.28.762254","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.28.762254","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/29/2026.10.28.762254.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/29/2026.10.28.762254.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035750330","display_name":"Robert Lodge","orcid":"https://orcid.org/0000-0002-9908-0850","observed_orcids":["https://orcid.org/0000-0002-9908-0850"]},"institutions":[{"id":"https://openalex.org/I4210158752","display_name":"Montreal Clinical Research Institute","ror":"https://ror.org/05m8pzq90","country_code":"CA","type":"facility","lineage":["https://openalex.org/I4210158752","https://openalex.org/I70931966"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Robert Lodge","raw_affiliation_strings":["Laboratory of Human Retrovirology, Institut de recherches cliniques de Montr\u00e9al (IRCM), Montreal, Quebec, Canada"],"raw_orcid":"https://orcid.org/0000-0002-9908-0850","affiliations":[{"raw_affiliation_string":"Laboratory of Human Retrovirology, Institut de recherches cliniques de Montr\u00e9al (IRCM), Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210158752"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049842711","display_name":"William G. Branton","orcid":"https://orcid.org/0000-0002-3259-2544","observed_orcids":["https://orcid.org/0000-0002-3259-2544"]},"institutions":[{"id":"https://openalex.org/I154425047","display_name":"University of Alberta","ror":"https://ror.org/0160cpw27","country_code":"CA","type":"education","lineage":["https://openalex.org/I154425047"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"William Branton","raw_affiliation_strings":["Department of Medicine and Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine and Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta, Canada","institution_ids":["https://openalex.org/I154425047"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075006919","display_name":"Frank Kirchhoff","orcid":"https://orcid.org/0000-0002-7052-2360","observed_orcids":["https://orcid.org/0000-0002-7052-2360"]},"institutions":[{"id":"https://openalex.org/I4210155144","display_name":"University Hospital Ulm","ror":"https://ror.org/05emabm63","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I4210155144"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Frank Kirchhoff","raw_affiliation_strings":["Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany"],"raw_orcid":"https://orcid.org/0000-0002-7052-2360","affiliations":[{"raw_affiliation_string":"Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany","institution_ids":["https://openalex.org/I4210155144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038583170","display_name":"Christopher Power","orcid":"https://orcid.org/0000-0002-5131-9711","observed_orcids":["https://orcid.org/0000-0002-5131-9711"]},"institutions":[{"id":"https://openalex.org/I154425047","display_name":"University of Alberta","ror":"https://ror.org/0160cpw27","country_code":"CA","type":"education","lineage":["https://openalex.org/I154425047"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Christopher Power","raw_affiliation_strings":["Department of Medicine and Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine and Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta, Canada","institution_ids":["https://openalex.org/I154425047"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025751053","display_name":"\u00c9ric A. Cohen","orcid":"https://orcid.org/0000-0003-1269-3791","observed_orcids":["https://orcid.org/0000-0003-1269-3791"]},"institutions":[{"id":"https://openalex.org/I4210158752","display_name":"Montreal Clinical Research Institute","ror":"https://ror.org/05m8pzq90","country_code":"CA","type":"facility","lineage":["https://openalex.org/I4210158752","https://openalex.org/I70931966"]},{"id":"https://openalex.org/I70931966","display_name":"Universit\u00e9 de Montr\u00e9al","ror":"https://ror.org/0161xgx34","country_code":"CA","type":"education","lineage":["https://openalex.org/I70931966"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"\u00c9ric A. Cohen","raw_affiliation_strings":["Department of Microbiology, Infectious Diseases and Immunology, Universit\u00e9 de Montr\u00e9al, Montreal, Quebec, Canada","Laboratory of Human Retrovirology, Institut de recherches cliniques de Montr\u00e9al (IRCM), Montreal, Quebec, Canada"],"raw_orcid":"https://orcid.org/0000-0003-1269-3791","affiliations":[{"raw_affiliation_string":"Department of Microbiology, Infectious Diseases and Immunology, Universit\u00e9 de Montr\u00e9al, Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I70931966"]},{"raw_affiliation_string":"Laboratory of Human Retrovirology, Institut de recherches cliniques de Montr\u00e9al (IRCM), Montreal, Quebec, Canada","institution_ids":["https://openalex.org/I4210158752"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5025751053"],"corresponding_institution_ids":["https://openalex.org/I4210158752","https://openalex.org/I70931966"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.04089999943971634,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hiv-1-infection","display_name":"HIV-1 infection","score":0.9539999961853027},{"id":"https://openalex.org/keywords/microglia","display_name":"microglia","score":0.9959999918937683},{"id":"https://openalex.org/keywords/g3bp2","display_name":"G3BP2","score":0.9929999709129333},{"id":"https://openalex.org/keywords/stress-granules","display_name":"stress granules","score":0.9980000257492065},{"id":"https://openalex.org/keywords/microrna-target-regulation","display_name":"microRNA target regulation","score":0.6909999847412109},{"id":"https://openalex.org/keywords/hiv-associated-neurocognitive-disorders","display_name":"HIV-associated neurocognitive disorders","score":0.8849999904632568},{"id":"https://openalex.org/keywords/hiv-reservoir","display_name":"HIV reservoir","score":0.6970000267028809},{"id":"https://openalex.org/keywords/microglial-transcriptome","display_name":"microglial transcriptome","score":0.7149999737739563},{"id":"https://openalex.org/keywords/hiv-replication","display_name":"HIV replication","score":0.7429999709129333},{"id":"https://openalex.org/keywords/host-antiviral-response","display_name":"host antiviral response","score":0.5870000123977661}],"concepts":[{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.7930999994277954},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.7548999786376953},{"id":"https://openalex.org/C164027704","wikidata":"https://www.wikidata.org/wiki/Q3491837","display_name":"Proinflammatory cytokine","level":3,"score":0.7427999973297119},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6585000157356262},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.6141999959945679},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.5406000018119812},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.4959000051021576},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4462999999523163},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42320001125335693},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4043000042438507},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.3831000030040741},{"id":"https://openalex.org/C2779244956","wikidata":"https://www.wikidata.org/wiki/Q184204","display_name":"Macrophage","level":3,"score":0.3564000129699707},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3447999954223633},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.33649998903274536},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3190000057220459},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2953999936580658},{"id":"https://openalex.org/C13373296","wikidata":"https://www.wikidata.org/wiki/Q421866","display_name":"Chemokine","level":3,"score":0.2948000133037567},{"id":"https://openalex.org/C2778690821","wikidata":"https://www.wikidata.org/wiki/Q212354","display_name":"Cytokine","level":2,"score":0.28610000014305115},{"id":"https://openalex.org/C3020196126","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammatory response","level":3,"score":0.27720001339912415},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.2662000060081482},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.265500009059906},{"id":"https://openalex.org/C529278444","wikidata":"https://www.wikidata.org/wiki/Q47273","display_name":"Central nervous system","level":2,"score":0.2590999901294708},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.25200000405311584}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.28.762254","doi":"https://doi.org/10.64898/2026.10.28.762254","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.28.762254","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/29/2026.10.28.762254.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.28.762254","doi":"https://doi.org/10.64898/2026.10.28.762254","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.28.762254","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/29/2026.10.28.762254.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.98580002784729,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3518280698","display_name":null,"funder_award_id":"164064","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"},{"id":"https://openalex.org/G3964012424","display_name":null,"funder_award_id":"154324","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"},{"id":"https://openalex.org/G885172923","display_name":null,"funder_award_id":"SFB1506","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320310638","display_name":"McGill University","ror":"https://ror.org/01pxwe438"},{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"},{"id":"https://openalex.org/F4320323175","display_name":"Universit\u00e9 de Montr\u00e9al","ror":"https://ror.org/0161xgx34"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7224576108.pdf","grobid_xml":"https://content.openalex.org/works/W7224576108.grobid-xml"},"referenced_works_count":73,"referenced_works":["https://openalex.org/W1797126752","https://openalex.org/W1837392183","https://openalex.org/W1929811921","https://openalex.org/W1948478725","https://openalex.org/W1983262897","https://openalex.org/W1991203356","https://openalex.org/W2015059296","https://openalex.org/W2019469658","https://openalex.org/W2034207391","https://openalex.org/W2060534902","https://openalex.org/W2063465748","https://openalex.org/W2078779212","https://openalex.org/W2080793236","https://openalex.org/W2100275083","https://openalex.org/W2112951712","https://openalex.org/W2126255146","https://openalex.org/W2128135205","https://openalex.org/W2133750999","https://openalex.org/W2142242761","https://openalex.org/W2142677020","https://openalex.org/W2170265194","https://openalex.org/W2170688212","https://openalex.org/W2253426984","https://openalex.org/W2301676073","https://openalex.org/W2321109448","https://openalex.org/W2330865564","https://openalex.org/W2527645962","https://openalex.org/W2734814984","https://openalex.org/W2737996197","https://openalex.org/W2761742990","https://openalex.org/W2782537156","https://openalex.org/W2790949745","https://openalex.org/W2792393222","https://openalex.org/W2891587714","https://openalex.org/W2922381878","https://openalex.org/W2964746641","https://openalex.org/W2970358232","https://openalex.org/W2973128481","https://openalex.org/W2981577557","https://openalex.org/W2997447385","https://openalex.org/W3016592635","https://openalex.org/W3016762840","https://openalex.org/W3021792538","https://openalex.org/W3038680026","https://openalex.org/W3080278846","https://openalex.org/W3115196011","https://openalex.org/W3122091881","https://openalex.org/W3126431621","https://openalex.org/W3216269705","https://openalex.org/W4210860317","https://openalex.org/W4220723100","https://openalex.org/W4221040770","https://openalex.org/W4290725198","https://openalex.org/W4300862647","https://openalex.org/W4318263963","https://openalex.org/W4380730918","https://openalex.org/W4382393748","https://openalex.org/W4384030047","https://openalex.org/W4387157056","https://openalex.org/W4391418252","https://openalex.org/W4392859090","https://openalex.org/W4396917318","https://openalex.org/W4399607715","https://openalex.org/W4401018405","https://openalex.org/W4405571072","https://openalex.org/W4406944041","https://openalex.org/W4408089472","https://openalex.org/W4409119012","https://openalex.org/W4410030133","https://openalex.org/W4411499328","https://openalex.org/W4415627798","https://openalex.org/W4416594434","https://openalex.org/W4417492483"],"related_works":["https://openalex.org/W3100305783","https://openalex.org/W2511019440","https://openalex.org/W2790156602","https://openalex.org/W6958856378","https://openalex.org/W7165938195","https://openalex.org/W2922381878","https://openalex.org/W7021180576","https://openalex.org/W3093326185","https://openalex.org/W3112884593","https://openalex.org/W4210860317"],"abstract_inverted_index":{"Abstract":[0],"Microglia":[1],"act":[2],"as":[3,73,87],"HIV":[4],"reservoirs":[5],"in":[6,65,101,111,137,157],"the":[7,12,23,56],"brain":[8],"and":[9,44,51,68,89,98,107,121,147,183],"contribute":[10],"to":[11,129],"development":[13],"of":[14,25,38,81,115],"HIV-associated":[15],"neurocognitive":[16],"disorders":[17],"despite":[18],"antiretroviral":[19],"therapy.":[20],"We":[21],"examined":[22],"transcriptome":[24],"primary":[26],"microglia":[27,159],"infected":[28,112,138],"ex":[29],"vivo":[30],"with":[31],"HIV-1.":[32],"Analyses":[33],"reveal":[34],"a":[35,52,78,140],"significant":[36],"modulation":[37],"DNA":[39],"damage/cell":[40],"cycle-,":[41],"cellular":[42],"stress-":[43],"non-membrane":[45],"bounded":[46],"organelle":[47],"assembly-related":[48],"gene":[49],"expression,":[50],"broad":[53],"effect":[54],"on":[55,174],"microRNA":[57],"profile.":[58],"MicroRNA-23b":[59,92,116],"is":[60,77,172],"identified":[61],"among":[62],"upregulated":[63],"microRNAs":[64],"HIV-1-infected":[66],"microglia,":[67,139],"downregulated":[69],"g3bp2":[70],"mRNA,":[71],"validated":[72],"its":[74],"target.":[75],"G3BP2":[76,96,169],"core":[79],"component":[80],"stress":[82,152,165],"granules":[83],"(SGs),":[84],"which":[85,163],"serve":[86],"antiviral":[88],"proinflammatory":[90],"platforms.":[91],"mimics":[93],"reduced":[94,122],"both":[95],"expression":[97,177],"SG":[99,119,135],"assembly":[100,136],"stress-induced":[102],"monocyte-derived":[103],"microglial":[104],"cells":[105],"(MDMi),":[106],"few":[108],"SGs":[109,182],"aggregate":[110],"MDMi.":[113],"Inhibition":[114],"partially":[117,180],"re-established":[118],"formation":[120],"HIV-1":[123,130,145,160,175,185],"replication.":[124],"Thus,":[125],"microRNA-23b":[126],"induction":[127],"contributes":[128],"strategies":[131],"aimed":[132],"at":[133],"counteracting":[134],"host":[141],"response":[142,153],"that":[143],"restricts":[144],"replication":[146,186],"ensuing":[148],"neuropathogenesis.":[149],"Highlights":[150],"Cellular":[151],"genes":[154],"are":[155],"modulated":[156],"HIV-infected":[158],"upregulates":[161],"miRNA-23b,":[162],"targets":[164],"granule":[166],"(SG)":[167],"factor":[168],"MiRNA-23b":[170],"upregulation":[171],"dependent":[173],"glycoproteins":[176],"Inhibiting":[178],"miRNA-23b":[179],"restores":[181],"reduces":[184]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-10-30T00:00:00"}