{"id":"https://openalex.org/W7224088844","doi":"https://doi.org/10.21037/tcr-2026-1764","title":"C18H17NO6 inhibits proliferation and induces apoptosis in human osteosarcoma U2OS cells via the PI3K/AKT signaling pathway","display_name":"C18H17NO6 inhibits proliferation and induces apoptosis in human osteosarcoma U2OS cells via the PI3K/AKT signaling pathway","publication_year":2026,"publication_date":"2026-10-01","ids":{"openalex":"https://openalex.org/W7224088844","doi":"https://doi.org/10.21037/tcr-2026-1764","pmid":"https://pubmed.ncbi.nlm.nih.gov/42997691","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13743231"},"language":"en","primary_location":{"id":"doi:10.21037/tcr-2026-1764","doi":"https://doi.org/10.21037/tcr-2026-1764","is_oa":true,"landing_page_url":"https://doi.org/10.21037/tcr-2026-1764","pdf_url":null,"source":{"id":"https://openalex.org/S2764965054","display_name":"Translational Cancer Research","issn_l":"2218-676X","issn":["2218-676X","2219-6803"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40","russia-white-list-4"],"host_organization":"https://openalex.org/P4310321814","host_organization_name":"AME Publishing Company","host_organization_lineage":["https://openalex.org/P4310321814"],"host_organization_lineage_names":["AME Publishing Company"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Translational Cancer Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.21037/tcr-2026-1764","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084039220","display_name":"Qianqian Qu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189210763","display_name":"Yunnan University","ror":"https://ror.org/0040axw97","country_code":"CN","type":"education","lineage":["https://openalex.org/I189210763"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qianqian Qu","raw_affiliation_strings":["Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China","institution_ids":["https://openalex.org/I189210763"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066819853","display_name":"Enzi Feng","orcid":"https://orcid.org/0000-0002-3410-2086","observed_orcids":["https://orcid.org/0000-0002-3410-2086"]},"institutions":[{"id":"https://openalex.org/I189210763","display_name":"Yunnan University","ror":"https://ror.org/0040axw97","country_code":"CN","type":"education","lineage":["https://openalex.org/I189210763"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Enzi Feng","raw_affiliation_strings":["Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China","institution_ids":["https://openalex.org/I189210763"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160055016","display_name":"Shiwen Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I26080491","display_name":"Kunming Medical University","ror":"https://ror.org/038c3w259","country_code":"CN","type":"education","lineage":["https://openalex.org/I26080491"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shiwen Zhang","raw_affiliation_strings":["Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, China","institution_ids":["https://openalex.org/I26080491"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022122149","display_name":"Zhongshun He","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210146235","display_name":"Yan'an Hospital Affiliated To Kunming Medical University","ror":"https://ror.org/05ctyj936","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210146235"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhongshun He","raw_affiliation_strings":["Department of Otolaryngology, Yan'an Hospital of Kunming City, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology, Yan'an Hospital of Kunming City, Kunming, China","institution_ids":["https://openalex.org/I4210146235"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160025371","display_name":"Jing Pan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210160531","display_name":"Chinese People's Liberation Army","ror":"https://ror.org/05tf9r976","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210160531"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jing Pan","raw_affiliation_strings":["Department of Otolaryngology, 920th Hospital, Joint Logistics Support Force of the Chinese People's Liberation Army, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology, 920th Hospital, Joint Logistics Support Force of the Chinese People's Liberation Army, Kunming, China","institution_ids":["https://openalex.org/I4210160531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160019347","display_name":"Jin Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189210763","display_name":"Yunnan University","ror":"https://ror.org/0040axw97","country_code":"CN","type":"education","lineage":["https://openalex.org/I189210763"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Jin Wang","raw_affiliation_strings":["Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China","institution_ids":["https://openalex.org/I189210763"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100839275","display_name":"Yanli Yang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189210763","display_name":"Yunnan University","ror":"https://ror.org/0040axw97","country_code":"CN","type":"education","lineage":["https://openalex.org/I189210763"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yanli Yang","raw_affiliation_strings":["Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Otolaryngology, Affiliated Hospital of Yunnan University, Kunming, China","institution_ids":["https://openalex.org/I189210763"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5100839275","https://openalex.org/A5160019347"],"corresponding_institution_ids":["https://openalex.org/I189210763"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.70207094,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"15","issue":"10","first_page":"739","last_page":"739"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.7139000296592712,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.7139000296592712,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10253","display_name":"Sarcoma Diagnosis and Treatment","score":0.07729999721050262,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12356","display_name":"Bioactive Compounds and Antitumor Agents","score":0.03009999915957451,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/usnic-acid","display_name":"usnic acid","score":0.8920000195503235},{"id":"https://openalex.org/keywords/osteosarcoma","display_name":"osteosarcoma","score":0.9900000095367432},{"id":"https://openalex.org/keywords/u2os-cells","display_name":"U2OS cells","score":0.9990000128746033},{"id":"https://openalex.org/keywords/pi3k-akt-signaling","display_name":"PI3K/Akt signaling","score":0.9139999747276306},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8140000104904175},{"id":"https://openalex.org/keywords/cell-proliferation-inhibition","display_name":"cell proliferation inhibition","score":0.7889999747276306},{"id":"https://openalex.org/keywords/s-phase-arrest","display_name":"S phase arrest","score":0.9359999895095825},{"id":"https://openalex.org/keywords/anticancer-activity","display_name":"anticancer activity","score":0.7919999957084656},{"id":"https://openalex.org/keywords/xenograft-tumor-growth","display_name":"xenograft tumor growth","score":0.6460000276565552},{"id":"https://openalex.org/keywords/740y-p","display_name":"740Y-P","score":0.8149999976158142},{"id":"https://openalex.org/keywords/ly294002","display_name":"LY294002","score":1.0}],"concepts":[{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.7839000225067139},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7468000054359436},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.6689000129699707},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.6661999821662903},{"id":"https://openalex.org/C86554907","wikidata":"https://www.wikidata.org/wiki/Q285613","display_name":"PI3K/AKT/mTOR pathway","level":3,"score":0.6498000025749207},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.6401000022888184},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5468000173568726},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5139999985694885},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4812000095844269},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.44679999351501465},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4117000102996826},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.39660000801086426},{"id":"https://openalex.org/C2776912716","wikidata":"https://www.wikidata.org/wiki/Q4042503","display_name":"LY294002","level":4,"score":0.38510000705718994},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.34779998660087585},{"id":"https://openalex.org/C2780278673","wikidata":"https://www.wikidata.org/wiki/Q476743","display_name":"Nude mouse","level":3,"score":0.31619998812675476},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.29330000281333923},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2802000045776367},{"id":"https://openalex.org/C105696609","wikidata":"https://www.wikidata.org/wiki/Q1068809","display_name":"Cell cycle checkpoint","level":4,"score":0.2662999927997589},{"id":"https://openalex.org/C75217442","wikidata":"https://www.wikidata.org/wiki/Q423650","display_name":"Protein kinase B","level":3,"score":0.26429998874664307}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.21037/tcr-2026-1764","doi":"https://doi.org/10.21037/tcr-2026-1764","is_oa":true,"landing_page_url":"https://doi.org/10.21037/tcr-2026-1764","pdf_url":null,"source":{"id":"https://openalex.org/S2764965054","display_name":"Translational Cancer Research","issn_l":"2218-676X","issn":["2218-676X","2219-6803"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40","russia-white-list-4"],"host_organization":"https://openalex.org/P4310321814","host_organization_name":"AME Publishing Company","host_organization_lineage":["https://openalex.org/P4310321814"],"host_organization_lineage_names":["AME Publishing Company"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Translational Cancer Research","raw_type":"journal-article"},{"id":"pmid:42997691","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42997691","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Translational cancer research","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.21037/tcr-2026-1764","doi":"https://doi.org/10.21037/tcr-2026-1764","is_oa":true,"landing_page_url":"https://doi.org/10.21037/tcr-2026-1764","pdf_url":null,"source":{"id":"https://openalex.org/S2764965054","display_name":"Translational Cancer Research","issn_l":"2218-676X","issn":["2218-676X","2219-6803"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40","russia-white-list-4"],"host_organization":"https://openalex.org/P4310321814","host_organization_name":"AME Publishing Company","host_organization_lineage":["https://openalex.org/P4310321814"],"host_organization_lineage_names":["AME Publishing Company"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Translational Cancer Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9476000070571899,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2979562838","https://openalex.org/W2808801716","https://openalex.org/W1996712795","https://openalex.org/W3086670389","https://openalex.org/W2900951106","https://openalex.org/W2406720304","https://openalex.org/W2963176761","https://openalex.org/W2563360691","https://openalex.org/W3174288651","https://openalex.org/W2365843339"],"abstract_inverted_index":{"Background:":[0],"C18H17NO6":[1,25,129,172,214],"is":[2],"a":[3,104,110,115,135],"dibenzofuran":[4],"derivative":[5],"of":[6,24,38,125,201,212],"(+)-usnic":[7],"acid":[8],"extracted":[9],"from":[10],"Usnea":[11],"lichens.":[12],"The":[13,193],"present":[14],"study":[15],"aimed":[16],"to":[17,121],"investigate":[18],"the":[19,45,123,126,169,198,205,210,226],"effects":[20,211,218],"and":[21,62,67,71,80,91,114,137,142,158,165,185,221],"potential":[22],"mechanisms":[23],"on":[26],"human":[27],"osteosarcoma":[28],"U2OS":[29,31,105,131],"cells.Methods:":[30],"cells":[32],"were":[33,86,101,119,187],"treated":[34],"with":[35],"different":[36],"concentrations":[37],"C18H17NO6.":[39],"Cell":[40,51],"proliferation":[41,133],"was":[42,53,75],"detected":[43],"using":[44,103],"cell":[46,72,132,145,152],"counting":[47],"kit-8":[48],"(CCK-8)":[49],"assay.":[50],"apoptosis":[52],"determined":[54],"by":[55,77,88,224],"TdT-mediated":[56],"dUTP":[57],"nick-end":[58],"labeling":[59],"(TUNEL)":[60],"staining":[61],"flow":[63],"cytometry":[64],"[FCM;":[65],"Annexin-V":[66],"propidium":[68],"iodide":[69],"(PI)],":[70],"cycle":[73,146],"distribution":[74],"analyzed":[76],"FCM.":[78],"Protein":[79],"messenger":[81],"RNA":[82],"(mRNA)":[83],"expression":[84],"levels":[85],"measured":[87],"western":[89],"blot":[90],"quantitative":[92],"real-time":[93],"polymerase":[94],"chain":[95],"reaction":[96],"(qRT-PCR).":[97],"In":[98,168],"vivo":[99,223],"experiments":[100],"performed":[102],"xenograft":[106,170],"mouse":[107],"model.":[108],"Additionally,":[109],"PI3K":[111,116,194,206],"agonist":[112,195],"(740Y-P)":[113],"inhibitor":[117,207],"(LY294002)":[118],"used":[120],"verify":[122],"involvement":[124],"PI3K/AKT":[127,202,227],"pathway.Results:":[128],"inhibited":[130],"in":[134,190,219,222],"dose-":[136],"time-dependent":[138],"manner,":[139],"induced":[140],"apoptosis,":[141],"caused":[143],"S-phase":[144],"arrest.":[147],"It":[148],"downregulated":[149,189],"Ki67,":[150],"proliferating":[151],"nuclear":[153],"antigen":[154],"(PCNA),":[155],"Bcl-2,":[156],"p-AKT,":[157],"p-PI3K,":[159],"while":[160],"upregulating":[161],"Bax,":[162],"cleaved":[163,166],"caspase-3,":[164],"caspase-9.":[167],"model,":[171],"suppressed":[173],"tumor":[174,191],"growth":[175],"without":[176],"significantly":[177],"affecting":[178],"body":[179],"weight":[180],"or":[181],"liver/kidney":[182],"function.":[183],"p-PI3K":[184],"p-AKT":[186],"also":[188],"tissues.":[192],"740Y-P":[196],"reversed":[197],"C18H17NO6-induced":[199],"downregulation":[200],"phosphorylation,":[203],"whereas":[204],"LY294002":[208],"mimicked":[209],"C18H17NO6.Conclusions:":[213],"exerts":[215],"significant":[216],"antitumor":[217],"vitro":[220],"inhibiting":[225],"signaling":[228],"pathway.":[229]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-29T00:00:00"}