{"id":"https://openalex.org/W7223649535","doi":"https://doi.org/10.1007/s12672-026-06219-y","title":"Integrating single-cell and spatial transcriptomics with computational perturbation identifies candidate prognostic genes associated with mitochondrial function and succinylation-related annotations in ovarian cancer","display_name":"Integrating single-cell and spatial transcriptomics with computational perturbation identifies candidate prognostic genes associated with mitochondrial function and succinylation-related annotations in ovarian cancer","publication_year":2026,"publication_date":"2026-10-26","ids":{"openalex":"https://openalex.org/W7223649535","doi":"https://doi.org/10.1007/s12672-026-06219-y"},"language":"en","primary_location":{"id":"doi:10.1007/s12672-026-06219-y","doi":"https://doi.org/10.1007/s12672-026-06219-y","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s12672-026-06219-y","pdf_url":null,"source":{"id":"https://openalex.org/S4210213696","display_name":"Discover Oncology","issn_l":"2730-6011","issn":["2730-6011"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Discover Oncology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1007/s12672-026-06219-y","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5126459587","display_name":"Yan Tong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210108480","display_name":"Second Affiliated Hospital of Nanchang University","ror":"https://ror.org/01nxv5c88","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210108480"]},{"id":"https://openalex.org/I4210121544","display_name":"Second Hospital of Nanchang","ror":"https://ror.org/0261d1g37","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210121544"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yan Tong","raw_affiliation_strings":["Center for Reproductive Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Reproductive Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China","institution_ids":["https://openalex.org/I4210108480","https://openalex.org/I4210121544"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5149975224","display_name":"Zhiqing Lai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210108480","display_name":"Second Affiliated Hospital of Nanchang University","ror":"https://ror.org/01nxv5c88","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210108480"]},{"id":"https://openalex.org/I4210121544","display_name":"Second Hospital of Nanchang","ror":"https://ror.org/0261d1g37","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210121544"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Zhiqing Lai","raw_affiliation_strings":["Department of Ultrasonic, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ultrasonic, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China","institution_ids":["https://openalex.org/I4210108480","https://openalex.org/I4210121544"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5149975224"],"corresponding_institution_ids":["https://openalex.org/I4210108480","https://openalex.org/I4210121544"],"apc_list":{"value":1690,"currency":"USD","value_usd":1690},"apc_paid":{"value":1690,"currency":"USD","value_usd":1690},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.80430761,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10550","display_name":"Ovarian cancer diagnosis and treatment","score":0.9082000255584717,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10550","display_name":"Ovarian cancer diagnosis and treatment","score":0.9082000255584717,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10515","display_name":"Cancer-related molecular mechanisms research","score":0.019200000911951065,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11297","display_name":"Ferroptosis and cancer prognosis","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ovarian-cancer","display_name":"ovarian cancer","score":1.0},{"id":"https://openalex.org/keywords/prognostic-genes","display_name":"prognostic genes","score":0.9259999990463257},{"id":"https://openalex.org/keywords/sirt3","display_name":"SIRT3","score":0.9990000128746033},{"id":"https://openalex.org/keywords/shmt2","display_name":"SHMT2","score":0.9900000095367432},{"id":"https://openalex.org/keywords/sirt5","display_name":"SIRT5","score":1.0},{"id":"https://openalex.org/keywords/risk-score","display_name":"risk score","score":0.7039999961853027},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.9660000205039978},{"id":"https://openalex.org/keywords/single-cell-rna-sequencing","display_name":"single-cell RNA sequencing","score":0.7919999957084656},{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.9919999837875366},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.7580000162124634},{"id":"https://openalex.org/keywords/patient-stratification","display_name":"patient stratification","score":0.6949999928474426}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7185999751091003},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.6898000240325928},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.6599000096321106},{"id":"https://openalex.org/C69991583","wikidata":"https://www.wikidata.org/wiki/Q1423575","display_name":"Candidate gene","level":3,"score":0.578499972820282},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5651999711990356},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5396999716758728},{"id":"https://openalex.org/C2780427987","wikidata":"https://www.wikidata.org/wiki/Q172341","display_name":"Ovarian cancer","level":3,"score":0.5060999989509583},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.4339999854564667},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.4165000021457672},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.3874000012874603},{"id":"https://openalex.org/C2776107976","wikidata":"https://www.wikidata.org/wiki/Q1786433","display_name":"Tumor microenvironment","level":3,"score":0.3709000051021576},{"id":"https://openalex.org/C2779399171","wikidata":"https://www.wikidata.org/wiki/Q1483951","display_name":"Malignancy","level":2,"score":0.36340001225471497},{"id":"https://openalex.org/C186836561","wikidata":"https://www.wikidata.org/wiki/Q1931516","display_name":"Microarray","level":4,"score":0.33230000734329224},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.32350000739097595},{"id":"https://openalex.org/C2779013556","wikidata":"https://www.wikidata.org/wiki/Q181876","display_name":"Metastasis","level":3,"score":0.3172000050544739},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3084999918937683},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3050999939441681},{"id":"https://openalex.org/C10515644","wikidata":"https://www.wikidata.org/wiki/Q543310","display_name":"Survival analysis","level":2,"score":0.29739999771118164},{"id":"https://openalex.org/C8415881","wikidata":"https://www.wikidata.org/wiki/Q6839217","display_name":"Microarray analysis techniques","level":4,"score":0.28870001435279846},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.2840999960899353},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.28360000252723694},{"id":"https://openalex.org/C179185449","wikidata":"https://www.wikidata.org/wiki/Q219699","display_name":"Suppressor","level":3,"score":0.27720001339912415},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.2770000100135803},{"id":"https://openalex.org/C3020404979","wikidata":"https://www.wikidata.org/wiki/Q1058438","display_name":"Risk stratification","level":2,"score":0.27399998903274536},{"id":"https://openalex.org/C19843653","wikidata":"https://www.wikidata.org/wiki/Q213907","display_name":"Model organism","level":3,"score":0.2727000117301941},{"id":"https://openalex.org/C2779256057","wikidata":"https://www.wikidata.org/wiki/Q16909647","display_name":"Tumor progression","level":3,"score":0.2671000063419342},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.263700008392334},{"id":"https://openalex.org/C89611455","wikidata":"https://www.wikidata.org/wiki/Q6804646","display_name":"Mechanism (biology)","level":2,"score":0.26159998774528503},{"id":"https://openalex.org/C2987395477","wikidata":"https://www.wikidata.org/wiki/Q135085","display_name":"Gene ontology","level":4,"score":0.2574000060558319},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2563999891281128}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/s12672-026-06219-y","doi":"https://doi.org/10.1007/s12672-026-06219-y","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s12672-026-06219-y","pdf_url":null,"source":{"id":"https://openalex.org/S4210213696","display_name":"Discover Oncology","issn_l":"2730-6011","issn":["2730-6011"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Discover Oncology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1007/s12672-026-06219-y","doi":"https://doi.org/10.1007/s12672-026-06219-y","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s12672-026-06219-y","pdf_url":null,"source":{"id":"https://openalex.org/S4210213696","display_name":"Discover Oncology","issn_l":"2730-6011","issn":["2730-6011"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Discover Oncology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9476000070571899,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7167074239","https://openalex.org/W4401501031","https://openalex.org/W4409955686","https://openalex.org/W7128422026","https://openalex.org/W4410456074","https://openalex.org/W4412573012","https://openalex.org/W4405871155","https://openalex.org/W7154451338","https://openalex.org/W4410236434","https://openalex.org/W7133343288"],"abstract_inverted_index":{"Ovarian":[0],"cancer":[1],"(OC)":[2],"is":[3,115,124,137,147],"the":[4,65,75,228],"deadliest":[5],"gynecological":[6],"malignancy":[7],"worldwide.":[8],"Whether":[9],"mitochondrial":[10],"succinylation\u2014an":[11],"important":[12],"post-translational":[13],"modification\u2014plays":[14],"any":[15],"functional":[16,186],"role":[17],"in":[18,43,59,118,139,150,164,182,195,236],"this":[19,26],"disease":[20],"remains":[21],"largely":[22],"unclear.":[23],"To":[24],"address":[25],"gap,":[27],"we":[28],"integrated":[29],"TCGA":[30],"and":[31,38,41,56,70,82,102,120,132,142,145,153,178,199,242],"GEO":[32],"transcriptomic":[33],"data":[34],"with":[35,105],"single-cell":[36],"RNA-seq":[37],"spatial":[39,68],"transcriptomics,":[40],"applied":[42],"silico":[44,196],"gene":[45],"perturbation":[46,197],"to":[47],"characterize":[48],"candidate":[49,86],"prognostic":[50,87],"genes":[51,161,190],"identified":[52,76,84],"from":[53,93,194,227],"overlapping":[54],"mitochondrial-function":[55],"succinylation-related":[57],"annotations":[58],"OC.":[60],"We":[61],"then":[62],"systematically":[63],"characterized":[64],"cellular":[66,205],"origins,":[67],"distributions,":[69],"potential":[71],"biological":[72],"functions":[73],"of":[74],"genes.":[77,88],"Four":[78],"genes\u2014ACSS3,":[79],"SIRT3,":[80],"SHMT2,":[81,177],"SIRT5\u2014were":[83],"as":[85],"A":[89],"risk":[90,232],"score":[91],"derived":[92,192],"their":[94,214],"expression":[95],"effectively":[96],"stratified":[97],"OC":[98,172],"patients":[99],"into":[100],"high-":[101],"low-risk":[103],"groups":[104],"markedly":[106],"different":[107],"survival":[108],"outcomes.":[109],"Single-cell":[110],"analysis":[111],"revealed":[112],"that":[113,159,175],"ACSS3":[114],"mainly":[116],"expressed":[117],"fibroblasts":[119],"epithelial":[121],"cells;":[122,144],"SIRT3":[123],"more":[125],"broadly":[126],"distributed":[127],"across":[128],"epithelial,":[129,140,151],"fibroblast,":[130,152],"endothelial,":[131,141],"T/NK":[133],"cell":[134],"populations;":[135],"SHMT2":[136],"enriched":[138,163],"fibroblast":[143],"SIRT5":[146,179],"predominantly":[148],"found":[149],"endothelial":[154],"cells.":[155],"Spatial":[156],"transcriptomics":[157],"confirmed":[158],"these":[160,189],"are":[162,180],"specific":[165],"tumor":[166],"microenvironment":[167],"regions.":[168],"RT-qPCR":[169],"on":[170],"clinical":[171],"specimens":[173],"verified":[174],"ACSS3,":[176],"overexpressed":[181],"tumors.":[183],"However,":[184],"all":[185],"inferences":[187],"regarding":[188],"were":[191],"solely":[193],"analyses":[198],"had":[200],"not":[201,224],"been":[202],"validated":[203],"by":[204],"knockdown/overexpression,":[206],"pharmacological":[207],"intervention,":[208],"or":[209,219],"animal":[210],"model":[211,233],"experiments;":[212],"accordingly,":[213],"direct":[215],"causal":[216],"regulatory":[217],"roles":[218],"therapeutic":[220],"target":[221],"value":[222],"could":[223],"be":[225],"established":[226],"present":[229],"data.":[230],"The":[231],"may":[234],"assist":[235],"patient":[237],"stratification":[238],"after":[239],"further":[240],"optimization":[241],"prospective":[243],"validation.":[244]},"counts_by_year":[],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2026-10-27T00:00:00"}