{"id":"https://openalex.org/W7223344571","doi":"https://doi.org/10.64898/2026.10.22.760787","title":"CST\u2013Pol\u03b1/primase, backed up by Rif1, protects telomeric ss-dsDNA junctions from damage recognition and resection","display_name":"CST\u2013Pol\u03b1/primase, backed up by Rif1, protects telomeric ss-dsDNA junctions from damage recognition and resection","publication_year":2026,"publication_date":"2026-10-23","ids":{"openalex":"https://openalex.org/W7223344571","doi":"https://doi.org/10.64898/2026.10.22.760787","pmid":"https://pubmed.ncbi.nlm.nih.gov/42996139","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13738707"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.22.760787","doi":"https://doi.org/10.64898/2026.10.22.760787","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.22.760787","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/23/2026.10.22.760787.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/23/2026.10.22.760787.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053361953","display_name":"Eun Young Yu","orcid":"https://orcid.org/0000-0003-4252-3443","observed_orcids":["https://orcid.org/0000-0003-4252-3443"]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eun Young Yu","raw_affiliation_strings":["Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America"],"raw_orcid":"https://orcid.org/0000-0003-4252-3443","affiliations":[{"raw_affiliation_string":"Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035838706","display_name":"Kimberly Calugaru","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kimberly Calugaru","raw_affiliation_strings":["Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5134639404","display_name":"Tenzin Younten","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tenzin Younten","raw_affiliation_strings":["Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159316547","display_name":"Sophie Huang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sophie Huang","raw_affiliation_strings":["Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065552028","display_name":"Neal F. Lue","orcid":"https://orcid.org/0000-0001-9700-6895","observed_orcids":["https://orcid.org/0000-0001-9700-6895"]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Neal F. Lue","raw_affiliation_strings":["Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America"],"raw_orcid":"https://orcid.org/0000-0001-9700-6895","affiliations":[{"raw_affiliation_string":"Department of Microbiology & Immunology, W. R. Hearst Microbiology Research Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","institution_ids":["https://openalex.org/I4387153466"]},{"raw_affiliation_string":"Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, 1300 York Avenue, New York, New York, 10065, United States of America","institution_ids":["https://openalex.org/I4387153466"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5065552028"],"corresponding_institution_ids":["https://openalex.org/I4387153466"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.5990999937057495,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.5990999937057495,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.38280001282691956,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cst-complex","display_name":"CST complex","score":0.8799999952316284},{"id":"https://openalex.org/keywords/cdc13","display_name":"Cdc13","score":0.8410000205039978},{"id":"https://openalex.org/keywords/rif1","display_name":"RIF1","score":0.9610000252723694},{"id":"https://openalex.org/keywords/telomere-protection","display_name":"telomere protection","score":0.9829999804496765},{"id":"https://openalex.org/keywords/dna-damage-recognition","display_name":"DNA damage recognition","score":0.7590000033378601},{"id":"https://openalex.org/keywords/dna-resection","display_name":"DNA resection","score":0.8389999866485596},{"id":"https://openalex.org/keywords/exo1","display_name":"EXO1","score":0.8920000195503235},{"id":"https://openalex.org/keywords/sgs1","display_name":"Sgs1","score":0.9580000042915344},{"id":"https://openalex.org/keywords/rad9","display_name":"Rad9","score":0.6890000104904175},{"id":"https://openalex.org/keywords/rad24","display_name":"Rad24","score":0.8980000019073486},{"id":"https://openalex.org/keywords/dna-damage-response","display_name":"DNA damage response","score":0.7580000162124634},{"id":"https://openalex.org/keywords/clamp-loader","display_name":"clamp loader","score":0.7680000066757202},{"id":"https://openalex.org/keywords/rnase-h1","display_name":"RNase H1","score":0.8090000152587891},{"id":"https://openalex.org/keywords/rnase-h2","display_name":"RNase H2","score":0.9890000224113464},{"id":"https://openalex.org/keywords/pot1","display_name":"POT1","score":0.9089999794960022},{"id":"https://openalex.org/keywords/candida-glabrata","display_name":"Candida glabrata","score":0.8690000176429749},{"id":"https://openalex.org/keywords/telomere-length-regulation","display_name":"telomere length regulation","score":0.6190000176429749}],"concepts":[{"id":"https://openalex.org/C177336024","wikidata":"https://www.wikidata.org/wiki/Q14872779","display_name":"Telomere","level":3,"score":0.9473000168800354},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.63919997215271},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6252999901771545},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5824000239372253},{"id":"https://openalex.org/C202265312","wikidata":"https://www.wikidata.org/wiki/Q7697810","display_name":"Telomere-binding protein","level":5,"score":0.582099974155426},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.5809999704360962},{"id":"https://openalex.org/C10879258","wikidata":"https://www.wikidata.org/wiki/Q76826611","display_name":"RNase P","level":4,"score":0.3986999988555908},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.38659998774528503},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3781000077724457},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.3741999864578247},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.36809998750686646},{"id":"https://openalex.org/C176944494","wikidata":"https://www.wikidata.org/wiki/Q1415380","display_name":"Point mutation","level":4,"score":0.3659000098705292},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.35740000009536743},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.3553999960422516},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.3515999913215637},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.3467000126838684},{"id":"https://openalex.org/C94581717","wikidata":"https://www.wikidata.org/wiki/Q331839","display_name":"Telomerase","level":3,"score":0.33899998664855957},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3253999948501587},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.3021000027656555},{"id":"https://openalex.org/C2776630724","wikidata":"https://www.wikidata.org/wiki/Q18031020","display_name":"RAD52","level":5,"score":0.29840001463890076},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.2838999927043915},{"id":"https://openalex.org/C178169997","wikidata":"https://www.wikidata.org/wiki/Q2892636","display_name":"Genome instability","level":4,"score":0.27090001106262207},{"id":"https://openalex.org/C105951970","wikidata":"https://www.wikidata.org/wiki/Q1036748","display_name":"Loss function","level":4,"score":0.2606000006198883},{"id":"https://openalex.org/C134494468","wikidata":"https://www.wikidata.org/wiki/Q408140","display_name":"Heteroduplex","level":3,"score":0.2590999901294708}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.10.22.760787","doi":"https://doi.org/10.64898/2026.10.22.760787","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.22.760787","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/23/2026.10.22.760787.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:42996139","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42996139","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13738707","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13738707/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.64898/2026.10.22.760787","doi":"https://doi.org/10.64898/2026.10.22.760787","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.22.760787","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/23/2026.10.22.760787.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7399690719","display_name":"The physical and functional interplay between telomere and repair proteins: mechanistic and evolutionary insights from an unconventional model","funder_award_id":"1817331","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7223344571.pdf","grobid_xml":"https://content.openalex.org/works/W7223344571.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4409459761","https://openalex.org/W2036332022","https://openalex.org/W7208728261","https://openalex.org/W1977997142","https://openalex.org/W68193126","https://openalex.org/W1593488935","https://openalex.org/W2747656558","https://openalex.org/W2011287318","https://openalex.org/W4200546981","https://openalex.org/W7099310901"],"abstract_inverted_index":{"Abstract":[0],"The":[1,81,138],"yeast":[2,41],"Cdc13-Stn1-Ten1":[3],"(CST)":[4],"complex":[5,68],"is":[6],"critical":[7],"for":[8,136],"telomere":[9,13,95,191,217,225,238,258,265],"length":[10],"regulation":[11],"and":[12,77,101,111,120,145,172,188,206,219,261],"protection.":[14,266],"Recent":[15],"studies":[16],"further":[17,233],"implicate":[18],"Pol\u03b1/primase":[19],"(PP)":[20],"as":[21,103,105,158],"an":[22],"essential":[23],"partner":[24],"of":[25,51,58,84,140,165,223,248,257,262],"CST":[26,119],"in":[27,72,94,162,174,195,229],"executing":[28],"these":[29],"functions,":[30],"but":[31],"the":[32,39,55,89,116,141,163,212,221,224,245,254],"mechanistic":[33,49],"bases":[34],"remain":[35],"poorly":[36],"understood.":[37],"Using":[38],"pathogenic":[40],"Nakaseomyces":[42],"glabratus":[43],",":[44],"a":[45,131,159,190,203],"model":[46],"amenable":[47],"to":[48,123,151,202],"dissection":[50],"CST-PP,":[52],"we":[53,198],"analyzed":[54],"protection":[56,134,218,246],"mechanisms":[57],"CST-PP":[59,90,263],"by":[60,109,128,179],"combining":[61],"mutations":[62,71,210],"that":[63,208,235],"disrupt":[64],"individual":[65],"subunits":[66],"or":[67],"assembly":[69,91],"with":[70,148,154,240],"DNA":[73,78,176,259],"damage":[74,96,260],"response":[75],"(DDR)":[76],"repair":[79],"factors.":[80],"complete":[82],"loss":[83],"Cdc13":[85,187,200],"\u2013":[86,92],"which":[87],"eliminates":[88],"results":[93],"recognition":[97],"involving":[98],"Rad9,":[99],"Rad24":[100],"Mec3,":[102],"well":[104],"aberrant":[106],"resection":[107],"mediated":[108,264],"Exo1":[110,149],"Sgs1.":[112],"In":[113],"contrast,":[114],"disrupting":[115],"interaction":[117,214],"between":[118,186],"PP":[121],"leads":[122],"proliferation":[124],"defects":[125],"triggered":[126],"primarily":[127],"Exo1,":[129],"implying":[130],"more":[132],"selective":[133],"function":[135,247],"PP.":[137],"involvement":[139],"DDR":[142],"clamp":[143,146],"loader":[144],"together":[147],"points":[150],"ss-dsDNA":[152,204],"junctions":[153],"recessed":[155],"5\u2019":[156,227],"ends":[157,228],"crucial":[160],"lesion":[161],"absence":[164],"Cdc13.":[166],"Further":[167],"analysis":[168],"implicates":[169],"RNase":[170],"H1":[171],"H2":[173],"promoting":[175],"damage,":[177],"possibly":[178],"exposing":[180],"such":[181],"junctions.":[182],"Based":[183],"on":[184],"similarities":[185],"POT1,":[189],"protein":[192,239],"previously":[193],"implicated":[194],"junctional":[196,241],"protection,":[197],"modeled":[199],"binding":[201,242],"junction":[205],"showed":[207],"point":[209],"at":[211],"proposed":[213],"site":[215],"impaired":[216],"altered":[220],"distribution":[222],"C-strand":[226],"vivo":[230],".":[231],"We":[232],"demonstrate":[234],"Rif1,":[236],"another":[237],"activity,":[243],"supports":[244],"CST-PP.":[249],"Together,":[250],"our":[251],"findings":[252],"illuminate":[253],"molecular":[255],"basis":[256]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-24T00:00:00"}