{"id":"https://openalex.org/W7223147376","doi":"https://doi.org/10.64898/2026.10.21.760667","title":"Cytoplasmic localization of 5hmC reveals RNA hydroxymethylation in breast cancer","display_name":"Cytoplasmic localization of 5hmC reveals RNA hydroxymethylation in breast cancer","publication_year":2026,"publication_date":"2026-10-21","ids":{"openalex":"https://openalex.org/W7223147376","doi":"https://doi.org/10.64898/2026.10.21.760667"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.21.760667","doi":"https://doi.org/10.64898/2026.10.21.760667","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.21.760667","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.760667.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.760667.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5022115575","display_name":"Jinrui Wen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jinrui Wen","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011287247","display_name":"Lisa Del Bel Belluz","orcid":"https://orcid.org/0000-0001-6012-9912","observed_orcids":["https://orcid.org/0000-0001-6012-9912"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Lisa Del Bel Belluz","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0001-6012-9912","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047573276","display_name":"Silvia Ottaviani","orcid":"https://orcid.org/0000-0002-8830-9947","observed_orcids":["https://orcid.org/0000-0002-8830-9947"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]},{"id":"https://openalex.org/I52590639","display_name":"Nottingham Trent University","ror":"https://ror.org/04xyxjd90","country_code":"GB","type":"education","lineage":["https://openalex.org/I52590639"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Silvia Ottaviani","raw_affiliation_strings":["Department of Biosciences, John van Geest Cancer Research Centre, Centre for Systems Health and Integrated Metabolic Research, School of Science and Technology, Nottingham Trent University, Nottingham, UK","Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0002-8830-9947","affiliations":[{"raw_affiliation_string":"Department of Biosciences, John van Geest Cancer Research Centre, Centre for Systems Health and Integrated Metabolic Research, School of Science and Technology, Nottingham Trent University, Nottingham, UK","institution_ids":["https://openalex.org/I52590639"]},{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013637834","display_name":"Fotini Poyia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201118511","display_name":"European University Cyprus","ror":"https://ror.org/04xp48827","country_code":"CY","type":"education","lineage":["https://openalex.org/I201118511"]},{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["CY","GB"],"is_corresponding":false,"raw_author_name":"Fotini Poyia","raw_affiliation_strings":["Basic & Translational Cancer Research Centre, Department of Life Sciences, European University Cyprus, Nicosia, Cyprus","Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Basic & Translational Cancer Research Centre, Department of Life Sciences, European University Cyprus, Nicosia, Cyprus","institution_ids":["https://openalex.org/I201118511"]},{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109677678","display_name":"Hemeng Yu","orcid":"https://orcid.org/0009-0000-2146-2595","observed_orcids":["https://orcid.org/0009-0000-2146-2595"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Hemeng Yu","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0009-0000-2146-2595","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015957877","display_name":"Saachi Sachdev","orcid":"https://orcid.org/0000-0002-8247-3287","observed_orcids":["https://orcid.org/0000-0002-8247-3287"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]},{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Saachi Sachdev","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom","UCL Respiratory, Division of Medicine, University College London, London, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]},{"raw_affiliation_string":"UCL Respiratory, Division of Medicine, University College London, London, United Kingdom","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027002959","display_name":"Liliane El Eid","orcid":"https://orcid.org/0000-0002-6511-6210","observed_orcids":["https://orcid.org/0000-0002-6511-6210"]},"institutions":[{"id":"https://openalex.org/I166337079","display_name":"Queen Mary University of London","ror":"https://ror.org/026zzn846","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I166337079"]},{"id":"https://openalex.org/I4210149920","display_name":"William Harvey Research Institute","ror":"https://ror.org/0574dzy90","country_code":"GB","type":"facility","lineage":["https://openalex.org/I124357947","https://openalex.org/I166337079","https://openalex.org/I4210149920"]},{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Liliane El Eid","raw_affiliation_strings":["Centre for Endocrinology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University London, London, United Kingdom","Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0002-6511-6210","affiliations":[{"raw_affiliation_string":"Centre for Endocrinology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University London, London, United Kingdom","institution_ids":["https://openalex.org/I166337079","https://openalex.org/I4210149920"]},{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159175167","display_name":"Katherine Garner","orcid":"https://orcid.org/0000-0001-9206-6266","observed_orcids":["https://orcid.org/0000-0001-9206-6266"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Katherine Garner","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0001-9206-6266","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159209691","display_name":"Krina Mistry","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Krina Mistry","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159207161","display_name":"Emily Hayes","orcid":"https://orcid.org/0009-0003-2904-3370","observed_orcids":["https://orcid.org/0009-0003-2904-3370"]},"institutions":[{"id":"https://openalex.org/I1325846038","display_name":"Royal Marsden NHS Foundation Trust","ror":"https://ror.org/0008wzh48","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1325846038"]},{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Emily Hayes","raw_affiliation_strings":["Centre for Molecular Pathology, The Royal Marsden NHS Foundation Trust Downs Road, Sutton, Surrey, SM2 5PT, United Kingdom","Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0009-0003-2904-3370","affiliations":[{"raw_affiliation_string":"Centre for Molecular Pathology, The Royal Marsden NHS Foundation Trust Downs Road, Sutton, Surrey, SM2 5PT, United Kingdom","institution_ids":["https://openalex.org/I1325846038"]},{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064442458","display_name":"Nina Moderau","orcid":"https://orcid.org/0000-0001-5656-839X","observed_orcids":["https://orcid.org/0000-0001-5656-839X"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Nina Moderau","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0001-5656-839X","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058216458","display_name":"Pramodh Chitral Chandrasinghe","orcid":"https://orcid.org/0000-0002-3485-961X","observed_orcids":["https://orcid.org/0000-0002-3485-961X"]},"institutions":[{"id":"https://openalex.org/I142651801","display_name":"University of Kelaniya","ror":"https://ror.org/02r91my29","country_code":"LK","type":"education","lineage":["https://openalex.org/I142651801"]},{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB","LK"],"is_corresponding":false,"raw_author_name":"Pramodh Chandrasinghe","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom","Department of Surgery, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka"],"raw_orcid":"https://orcid.org/0000-0002-3485-961X","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]},{"raw_affiliation_string":"Department of Surgery, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka","institution_ids":["https://openalex.org/I142651801"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079409805","display_name":"Justin Stebbing","orcid":"https://orcid.org/0000-0002-1117-6947","observed_orcids":["https://orcid.org/0000-0002-1117-6947"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]},{"id":"https://openalex.org/I51216347","display_name":"Anglia Ruskin University","ror":"https://ror.org/0009t4v78","country_code":"GB","type":"education","lineage":["https://openalex.org/I51216347"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Justin Stebbing","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom","School of Life Sciences, Anglia Ruskin University, Cambridge, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0002-1117-6947","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]},{"raw_affiliation_string":"School of Life Sciences, Anglia Ruskin University, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I51216347"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055025757","display_name":"Biancastella Cereser","orcid":"https://orcid.org/0000-0003-1243-1767","observed_orcids":["https://orcid.org/0000-0003-1243-1767"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Biancastella Cereser","raw_affiliation_strings":["Department of Surgery and Cancer, Imperial College London, London, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0003-1243-1767","affiliations":[{"raw_affiliation_string":"Department of Surgery and Cancer, Imperial College London, London, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":9,"corresponding_author_ids":["https://openalex.org/A5055025757"],"corresponding_institution_ids":["https://openalex.org/I47508984"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.6269999742507935,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.6269999742507935,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.34299999475479126,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.004999999888241291,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/5-hydroxymethylcytosine","display_name":"5-hydroxymethylcytosine","score":0.9929999709129333},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.824999988079071},{"id":"https://openalex.org/keywords/tet3","display_name":"TET3","score":0.8080000281333923},{"id":"https://openalex.org/keywords/tet2","display_name":"TET2","score":0.9909999966621399},{"id":"https://openalex.org/keywords/triple-negative-breast-cancer","display_name":"triple-negative breast cancer","score":0.9229999780654907},{"id":"https://openalex.org/keywords/dna-methylation","display_name":"DNA methylation","score":0.5730000138282776},{"id":"https://openalex.org/keywords/rna-splicing","display_name":"RNA splicing","score":0.6520000100135803},{"id":"https://openalex.org/keywords/transcript-stability","display_name":"transcript stability","score":0.4569999873638153},{"id":"https://openalex.org/keywords/digital-pathology","display_name":"digital pathology","score":0.5649999976158142}],"concepts":[{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.7059999704360962},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6974999904632568},{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.6114000082015991},{"id":"https://openalex.org/C530470458","wikidata":"https://www.wikidata.org/wiki/Q128581","display_name":"Breast cancer","level":3,"score":0.5455999970436096},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5303999781608582},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5149000287055969},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.5094000101089478},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.4925999939441681},{"id":"https://openalex.org/C190727270","wikidata":"https://www.wikidata.org/wiki/Q874745","display_name":"DNA methylation","level":4,"score":0.4708999991416931},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.4415000081062317},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.41990000009536743},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.41839998960494995},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.3991999924182892},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36649999022483826},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.3540000021457672},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3476000130176544},{"id":"https://openalex.org/C2994423619","wikidata":"https://www.wikidata.org/wiki/Q9103","display_name":"Human breast","level":4,"score":0.3301999866962433},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3203999996185303},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3100999891757965},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.30300000309944153},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.2761000096797943},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.27469998598098755},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2680000066757202},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.2572000026702881}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.21.760667","doi":"https://doi.org/10.64898/2026.10.21.760667","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.21.760667","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.760667.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.21.760667","doi":"https://doi.org/10.64898/2026.10.21.760667","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.21.760667","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.760667.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8199999928474426}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320319990","display_name":"National Institute for Health and Care Research","ror":"https://ror.org/0187kwz08"},{"id":"https://openalex.org/F4320320283","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10"},{"id":"https://openalex.org/F4320332102","display_name":"Action Against Cancer","ror":"https://ror.org/04pz0a731"},{"id":"https://openalex.org/F4320333939","display_name":"Imperial Experimental Cancer Medicine Centre","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7223147376.pdf","grobid_xml":"https://content.openalex.org/works/W7223147376.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7116302048","https://openalex.org/W4296468178","https://openalex.org/W2358488663","https://openalex.org/W2103310687","https://openalex.org/W2886707895","https://openalex.org/W3020973596","https://openalex.org/W2885588068","https://openalex.org/W4408016466","https://openalex.org/W4402454144","https://openalex.org/W1982704134"],"abstract_inverted_index":{"Abstract":[0],"DNA":[1],"methylation":[2],"(5-methylcytosine,":[3],"5mC)":[4],"is":[5],"a":[6,47,112,132],"major":[7],"repressive":[8],"epigenetic":[9],"mark":[10],"that":[11,76],"can":[12,28],"be":[13],"iteratively":[14],"oxidized":[15],"by":[16],"ten-eleven-translocation":[17],"(TET)":[18],"enzymes":[19],"to":[20],"5-hydroxymethylcytosine":[21],"(5hmC);":[22],"beyond":[23],"DNA,":[24],"modifications":[25],"of":[26,81,134],"RNA":[27,36,83],"also":[29],"affect":[30],"transcript":[31],"stability":[32],"and":[33,65,68,84,94,98,106,123],"processing,":[34],"yet":[35],"hydroxymethylation":[37,80],"remains":[38],"unexplored":[39],"in":[40,52,59,91,104,109,126],"breast":[41,142],"cancer":[42,143],"(BC).":[43],"Here,":[44],"we":[45],"identify":[46],"quantifiable":[48],"cytoplasmic":[49,102],"5hmC":[50,87],"signal":[51,78],"BC":[53,61],"cell":[54,129],"lines":[55,130],"and,":[56],"more":[57],"prominently,":[58],"human":[60],"tissue,":[62],"using":[63],"immunoassays":[64],"digital":[66],"pathology,":[67],"confirm":[69],"with":[70,100,120],"an":[71],"adapted,":[72],"RNase-sensitive,":[73],"RNA-specific":[74],"ELISA":[75],"this":[77],"reflects":[79],"total":[82],"mRNA.":[85],"Cytoplasmic":[86],"was":[88,118],"significantly":[89],"enriched":[90],"triple-negative":[92],"(ER-/PR-/HER2-)":[93],"grade":[95],"3":[96],"tumors,":[97],"correlated":[99],"increased":[101],"TET2/TET3":[103],"tissue":[105],"TET3":[107,116,124],"upregulation":[108],"TCGA.":[110],"As":[111],"leading":[113],"candidate":[114],"mechanism,":[115],"expression":[117],"associated":[119],"RNA-processing/spliceosomal":[121],"transcripts,":[122],"knockdown":[125],"three":[127],"TNBC":[128],"reduced":[131],"subset":[133],"these":[135],"targets,":[136],"independently":[137],"supported":[138],"across":[139],"70":[140],"DepMap":[141],"lines.":[144]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-22T00:00:00"}