{"id":"https://openalex.org/W7223113229","doi":"https://doi.org/10.64898/2026.10.14.759149","title":"Stimulating microglial neuroinflammation with amyloid-beta oligomers compatible with solid-state NMR structural characterization","display_name":"Stimulating microglial neuroinflammation with amyloid-beta oligomers compatible with solid-state NMR structural characterization","publication_year":2026,"publication_date":"2026-10-20","ids":{"openalex":"https://openalex.org/W7223113229","doi":"https://doi.org/10.64898/2026.10.14.759149","pmid":"https://pubmed.ncbi.nlm.nih.gov/42995837","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13738396"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.14.759149","doi":"https://doi.org/10.64898/2026.10.14.759149","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.14.759149","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/20/2026.10.14.759149.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/20/2026.10.14.759149.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5104632374","display_name":"Daniel M. Dinakarapandian","orcid":"https://orcid.org/0009-0000-0081-3958","observed_orcids":["https://orcid.org/0009-0000-0081-3958"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel M Dinakarapandian","raw_affiliation_strings":["School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA","institution_ids":["https://openalex.org/I130701444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113051161","display_name":"Tarunya Rao Sudarshan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tarunya Rao Sudarshan","raw_affiliation_strings":["School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA","institution_ids":["https://openalex.org/I130701444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104326826","display_name":"Martin N. Griffin","orcid":"https://orcid.org/0009-0006-9671-4674","observed_orcids":["https://orcid.org/0009-0006-9671-4674"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin N Griffin","raw_affiliation_strings":["George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","The Graduate Program in Neuroscience, Emory University, Atlanta, GA 30322, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"The Graduate Program in Neuroscience, Emory University, Atlanta, GA 30322, USA","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159171671","display_name":"Pranay Ramaswamy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pranay Ramaswamy","raw_affiliation_strings":["School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA","institution_ids":["https://openalex.org/I130701444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159188998","display_name":"Phuong H Nguyen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Phuong H Nguyen","raw_affiliation_strings":["School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA","institution_ids":["https://openalex.org/I130701444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109103938","display_name":"Tzu-Ying Chiu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tzu-Ying Chiu","raw_affiliation_strings":["School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA","institution_ids":["https://openalex.org/I130701444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5133491116","display_name":"Chenxing Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]},{"id":"https://openalex.org/I2802612298","display_name":"The Wallace H. Coulter Department of Biomedical Engineering","ror":"https://ror.org/02j15s898","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chenxing Li","raw_affiliation_strings":["George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106246883","display_name":"S. Shirin Kamalaldinezabadi","orcid":"https://orcid.org/0000-0001-9446-7194","observed_orcids":["https://orcid.org/0000-0001-9446-7194"]},"institutions":[{"id":"https://openalex.org/I103163165","display_name":"Florida State University","ror":"https://ror.org/05g3dte14","country_code":"US","type":"education","lineage":["https://openalex.org/I103163165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S. Shirin Kamalaldinezabadi","raw_affiliation_strings":["Institute of Molecular Biophysics, Florida State University, Tallahassee, FL, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Biophysics, Florida State University, Tallahassee, FL, USA","institution_ids":["https://openalex.org/I103163165"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058101047","display_name":"Jens O. Watzlawik","orcid":"https://orcid.org/0000-0003-0610-0267","observed_orcids":["https://orcid.org/0000-0003-0610-0267"]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]},{"id":"https://openalex.org/I4210146710","display_name":"Mayo Clinic in Florida","ror":"https://ror.org/03zzw1w08","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723","https://openalex.org/I4210146710"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jens O Watzlawik","raw_affiliation_strings":["Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA","institution_ids":["https://openalex.org/I1330342723","https://openalex.org/I4210146710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002695228","display_name":"Terrone L. Rosenberry","orcid":"https://orcid.org/0000-0003-3579-7681","observed_orcids":["https://orcid.org/0000-0003-3579-7681"]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]},{"id":"https://openalex.org/I4210146710","display_name":"Mayo Clinic in Florida","ror":"https://ror.org/03zzw1w08","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723","https://openalex.org/I4210146710"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Terrone L Rosenberry","raw_affiliation_strings":["Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA","institution_ids":["https://openalex.org/I1330342723","https://openalex.org/I4210146710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068698824","display_name":"Scott M. Stagg","orcid":"https://orcid.org/0000-0001-8855-4152","observed_orcids":["https://orcid.org/0000-0001-8855-4152"]},"institutions":[{"id":"https://openalex.org/I103163165","display_name":"Florida State University","ror":"https://ror.org/05g3dte14","country_code":"US","type":"education","lineage":["https://openalex.org/I103163165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott M Stagg","raw_affiliation_strings":["Institute of Molecular Biophysics, Florida State University, Tallahassee, FL, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Biophysics, Florida State University, Tallahassee, FL, USA","institution_ids":["https://openalex.org/I103163165"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159176560","display_name":"Zhexing Wen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhexing Wen","raw_affiliation_strings":["Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, USA","Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA","Department of Neurology, Emory University School of Medicine, Atlanta, GA, USA","Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, GA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, USA","institution_ids":["https://openalex.org/I150468666"]},{"raw_affiliation_string":"Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA","institution_ids":["https://openalex.org/I150468666"]},{"raw_affiliation_string":"Department of Neurology, Emory University School of Medicine, Atlanta, GA, USA","institution_ids":["https://openalex.org/I150468666"]},{"raw_affiliation_string":"Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, GA, USA","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067247177","display_name":"Levi Benjamin Wood","orcid":"https://orcid.org/0000-0002-4248-0888","observed_orcids":["https://orcid.org/0000-0002-4248-0888"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]},{"id":"https://openalex.org/I2802612298","display_name":"The Wallace H. Coulter Department of Biomedical Engineering","ror":"https://ror.org/02j15s898","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Levi B Wood","raw_affiliation_strings":["George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, 30332, USA","Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, 30332, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062697029","display_name":"Anant K. Paravastu","orcid":"https://orcid.org/0000-0001-7183-1942","observed_orcids":["https://orcid.org/0000-0001-7183-1942"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Anant K Paravastu","raw_affiliation_strings":["Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, 30332, USA","School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, 30332, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA","institution_ids":["https://openalex.org/I130701444"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5062697029"],"corresponding_institution_ids":["https://openalex.org/I130701444"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10009","display_name":"Dementia and Cognitive Impairment Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2738","display_name":"Psychiatry and Mental health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/amyloid-beta-oligomers","display_name":"amyloid-beta oligomers","score":0.968999981880188},{"id":"https://openalex.org/keywords/amyloid-beta-1-42","display_name":"amyloid-beta 42","score":0.875},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.8709999918937683},{"id":"https://openalex.org/keywords/microglial-neuroinflammation","display_name":"microglial neuroinflammation","score":0.9419999718666077},{"id":"https://openalex.org/keywords/oligomeric-structure","display_name":"oligomeric structure","score":0.6159999966621399},{"id":"https://openalex.org/keywords/beta-sheet","display_name":"beta-sheet","score":0.6570000052452087},{"id":"https://openalex.org/keywords/neurotoxicity","display_name":"neurotoxicity","score":0.6380000114440918}],"concepts":[{"id":"https://openalex.org/C2780642029","wikidata":"https://www.wikidata.org/wiki/Q726004","display_name":"Oligomer","level":2,"score":0.9677000045776367},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.7991999983787537},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7023000121116638},{"id":"https://openalex.org/C87753298","wikidata":"https://www.wikidata.org/wiki/Q17157137","display_name":"Neuroinflammation","level":3,"score":0.656499981880188},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5123999714851379},{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.4918000102043152},{"id":"https://openalex.org/C66974803","wikidata":"https://www.wikidata.org/wiki/Q10359898","display_name":"Nuclear magnetic resonance spectroscopy","level":2,"score":0.4629000127315521},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4471000134944916},{"id":"https://openalex.org/C116073593","wikidata":"https://www.wikidata.org/wiki/Q864491","display_name":"Biological activity","level":3,"score":0.415800005197525},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.4050000011920929},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3894999921321869},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.37459999322891235},{"id":"https://openalex.org/C132677234","wikidata":"https://www.wikidata.org/wiki/Q3273544","display_name":"Structural motif","level":2,"score":0.32749998569488525},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3264000117778778},{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.3262999951839447},{"id":"https://openalex.org/C103319777","wikidata":"https://www.wikidata.org/wiki/Q1979695","display_name":"Proton NMR","level":2,"score":0.298799991607666},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.2858000099658966},{"id":"https://openalex.org/C163111631","wikidata":"https://www.wikidata.org/wiki/Q1952454","display_name":"Carbon-13 NMR","level":2,"score":0.28189998865127563},{"id":"https://openalex.org/C19549132","wikidata":"https://www.wikidata.org/wiki/Q11141463","display_name":"Two-dimensional nuclear magnetic resonance spectroscopy","level":2,"score":0.26759999990463257},{"id":"https://openalex.org/C2778690821","wikidata":"https://www.wikidata.org/wiki/Q212354","display_name":"Cytokine","level":2,"score":0.26570001244544983},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.2653000056743622}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.10.14.759149","doi":"https://doi.org/10.64898/2026.10.14.759149","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.14.759149","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/20/2026.10.14.759149.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:42995837","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42995837","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13738396","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13738396/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.64898/2026.10.14.759149","doi":"https://doi.org/10.64898/2026.10.14.759149","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.14.759149","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/20/2026.10.14.759149.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9537000060081482,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7223113229.pdf","grobid_xml":"https://content.openalex.org/works/W7223113229.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W4415487392"],"related_works":["https://openalex.org/W1991647496","https://openalex.org/W2078981524","https://openalex.org/W2093684160","https://openalex.org/W7024810812","https://openalex.org/W3029456209","https://openalex.org/W7131304660","https://openalex.org/W2970963997","https://openalex.org/W2013937856","https://openalex.org/W2897248524","https://openalex.org/W2056593588"],"abstract_inverted_index":{"Soluble":[0],"amyloid-\u03b2":[1],"(A\u03b2)":[2],"oligomers":[3,115,158,188],"are":[4,48],"considered":[5],"the":[6,14,100,143,213,221],"primary":[7],"neurotoxic":[8],"species":[9],"in":[10,37,58,69,195,204,246],"Alzheimer's":[11],"disease,":[12],"yet":[13],"relationship":[15],"between":[16],"oligomer":[17,46,232],"structure":[18,80],"and":[19,72,93,123,127,140,177,243],"biological":[20],"activity":[21,194,210],"remains":[22],"poorly":[23],"understood.":[24],"Oligomer":[25],"sample":[26],"preparations":[27],"amenable":[28],"to":[29,44,50,139,257],"high-resolution":[30],"structural":[31,241],"characterization":[32,76,242],"have":[33],"not":[34],"been":[35],"tested":[36],"cell-based":[38],"assays,":[39,248],"mostly":[40],"because":[41],"detergents":[42],"used":[43],"drive":[45],"formation":[47],"toxic":[49],"cells.":[51],"Here,":[52],"we":[53],"describe":[54],"a":[55,70,78,84,160,196,229,250],"detergent-free":[56,230],"protocol":[57],"which":[59],"size-exclusion":[60],"chromatography-isolated":[61],"A\u03b242":[62,201,217,231],"monomers":[63],"spontaneously":[64],"oligomerize":[65],"at":[66],"37":[67,113,156],"\u00b0C":[68,114,157],"concentration-":[71],"temperature-dependent":[73],"manner.":[74],"Biophysical":[75],"shows":[77],"\u03b2-sheet":[79],"by":[81,90,96,173],"circular":[82],"dichroism,":[83],"self-associating":[85],"population":[86],"above":[87],"100":[88],"kDa":[89,105,145],"silver-stained":[91],"SDS-PAGE,":[92],"bead-like":[94],"strings":[95],"negative-stain":[97],"TEM":[98],"resembling":[99],"previously":[101],"characterized":[102],"SDS-induced":[103],"150":[104,144],"oligomer.":[106,146],"Solid-state":[107],"NMR":[108,240],"spectroscopy":[109],"of":[110,148,168,216,223],"selectively":[111],"\u00b9\u00b3C/\u00b9\u2075N-labeled":[112],"yields":[116],"well-resolved":[117],"spectra":[118],"with":[119,153,185,238],"\u03b2-strand":[120],"chemical":[121],"shifts,":[122],"two-dimensional":[124],"\u00b9\u00b3C-\u00b9\u00b3C":[125],"DARR":[126],"CHHC":[128],"experiments":[129],"provide":[130],"initial":[131],"inter-residue":[132],"distance":[133],"constraints":[134],"that":[135,165,167,208,234],"reveal":[136],"both":[137,205],"similarities":[138],"differences":[141],"from":[142,191],"Treatment":[147],"mouse":[149],"microglial":[150,254],"cells":[151],"(MMCs)":[152],"50":[154],"nM":[155],"elicited":[159],"robust":[161],"pro-inflammatory":[162],"cytokine":[163],"response":[164],"exceeded":[166],"monomeric":[169],"A\u03b242,":[170],"as":[171],"demonstrated":[172],"Luminex":[174],"multiplex":[175],"profiling":[176],"principal":[178],"component":[179],"analysis.":[180],"hiPSC-derived":[181],"microglia":[182],"(iMGs)":[183],"treated":[184],"1":[186],"\u00b5M":[187],"also":[189],"separated":[190],"vehicle,":[192],"establishing":[193],"human":[197],"cellular":[198],"model.":[199],"Monomeric":[200],"was":[202],"inactive":[203],"systems,":[206],"indicating":[207],"inflammatory":[209],"depends":[211],"on":[212,220],"assembly":[214],"state":[215],"rather":[218],"than":[219],"presence":[222],"peptide":[224],"alone.":[225],"These":[226],"results":[227],"establish":[228],"preparation":[233],"is":[235],"simultaneously":[236],"compatible":[237],"solid-state":[239],"biologically":[244],"active":[245],"neuroinflammation":[247],"providing":[249],"platform":[251],"for":[252],"studying":[253],"immune":[255],"responses":[256],"oligomers.":[258]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-22T00:00:00"}