{"id":"https://openalex.org/W7223088694","doi":"https://doi.org/10.64898/2026.10.21.759893","title":"\u03b2-Carbonic anhydrase Sl\u03b2CA4 is required for normal growth, carbon assimilation and stomatal plasticity in tomato","display_name":"\u03b2-Carbonic anhydrase Sl\u03b2CA4 is required for normal growth, carbon assimilation and stomatal plasticity in tomato","publication_year":2026,"publication_date":"2026-10-21","ids":{"openalex":"https://openalex.org/W7223088694","doi":"https://doi.org/10.64898/2026.10.21.759893"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.21.759893","doi":"https://doi.org/10.64898/2026.10.21.759893","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.21.759893","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.759893.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.759893.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5159162084","display_name":"Orr Meyers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I13955877","display_name":"Bar-Ilan University","ror":"https://ror.org/03kgsv495","country_code":"IL","type":"education","lineage":["https://openalex.org/I13955877"]},{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Orr Meyers","raw_affiliation_strings":["Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","institution_ids":["https://openalex.org/I376102"]},{"raw_affiliation_string":"The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel","institution_ids":["https://openalex.org/I13955877"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159176962","display_name":"Mia Stern Tal","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I197251160","display_name":"Hebrew University of Jerusalem","ror":"https://ror.org/03qxff017","country_code":"IL","type":"education","lineage":["https://openalex.org/I197251160"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Mia Stern Tal","raw_affiliation_strings":["Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel","institution_ids":["https://openalex.org/I197251160"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5116457729","display_name":"Jyoti Singh","orcid":"https://orcid.org/0009-0000-8417-5243","observed_orcids":["https://orcid.org/0009-0000-8417-5243"]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Jyoti Singh","raw_affiliation_strings":["Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159213000","display_name":"Yuval Nagar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Yuval Nagar","raw_affiliation_strings":["Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049262385","display_name":"Iris Biton","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Iris Biton","raw_affiliation_strings":["Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030286176","display_name":"Adi Yaaran","orcid":"https://orcid.org/0000-0001-9343-9976","observed_orcids":["https://orcid.org/0000-0001-9343-9976","https://orcid.org/0000-0003-1876-1484"]},"institutions":[{"id":"https://openalex.org/I197251160","display_name":"Hebrew University of Jerusalem","ror":"https://ror.org/03qxff017","country_code":"IL","type":"education","lineage":["https://openalex.org/I197251160"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Adi Yarran","raw_affiliation_strings":["Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel"],"raw_orcid":"https://orcid.org/0000-0001-9343-9976","affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel","institution_ids":["https://openalex.org/I197251160"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050249373","display_name":"Yaarit Kutsher","orcid":"https://orcid.org/0000-0002-9403-8362","observed_orcids":["https://orcid.org/0000-0002-9403-8362"]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Yaarit Kutsher","raw_affiliation_strings":["Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","institution_ids":["https://openalex.org/I376102"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035231567","display_name":"Yotam Zait","orcid":"https://orcid.org/0000-0003-4266-1635","observed_orcids":["https://orcid.org/0000-0003-4266-1635"]},"institutions":[{"id":"https://openalex.org/I197251160","display_name":"Hebrew University of Jerusalem","ror":"https://ror.org/03qxff017","country_code":"IL","type":"education","lineage":["https://openalex.org/I197251160"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Yotam Zait","raw_affiliation_strings":["Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel"],"raw_orcid":"https://orcid.org/0000-0003-4266-1635","affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel","institution_ids":["https://openalex.org/I197251160"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5010276966","display_name":"Ido Nir","orcid":"https://orcid.org/0000-0002-6223-6700","observed_orcids":["https://orcid.org/0000-0002-6223-6700"]},"institutions":[{"id":"https://openalex.org/I376102","display_name":"Agricultural Research Organization","ror":"https://ror.org/05hbrxp80","country_code":"IL","type":"government","lineage":["https://openalex.org/I376102"]}],"countries":["IL"],"is_corresponding":true,"raw_author_name":"Ido Nir","raw_affiliation_strings":["Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel"],"raw_orcid":"https://orcid.org/0000-0002-6223-6700","affiliations":[{"raw_affiliation_string":"Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion 7505101, Israel","institution_ids":["https://openalex.org/I376102"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5010276966"],"corresponding_institution_ids":["https://openalex.org/I376102"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12412","display_name":"Enzyme function and inhibition","score":0.8988999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12412","display_name":"Enzyme function and inhibition","score":0.8988999724388123,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.0820000022649765,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.0071000000461936,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-carbonic-anhydrase","display_name":"beta-carbonic anhydrase","score":0.9620000123977661},{"id":"https://openalex.org/keywords/tomato","display_name":"tomato","score":0.7540000081062317},{"id":"https://openalex.org/keywords/co2-assimilation","display_name":"carbon assimilation","score":0.8009999990463257},{"id":"https://openalex.org/keywords/stomatal-conductance","display_name":"stomatal conductance","score":0.7200000286102295},{"id":"https://openalex.org/keywords/photosynthesis","display_name":"photosynthesis","score":0.8159999847412109},{"id":"https://openalex.org/keywords/elevated-co2","display_name":"elevated CO2","score":0.6990000009536743},{"id":"https://openalex.org/keywords/high-temperature","display_name":"high temperature","score":0.699999988079071},{"id":"https://openalex.org/keywords/stomatal-index","display_name":"stomatal index","score":0.8980000019073486},{"id":"https://openalex.org/keywords/plant-growth","display_name":"plant growth","score":0.5070000290870667},{"id":"https://openalex.org/keywords/bicarbonate","display_name":"bicarbonate","score":0.3889999985694885}],"concepts":[{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6360999941825867},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.6121000051498413},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6039000153541565},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.5303999781608582},{"id":"https://openalex.org/C2781045423","wikidata":"https://www.wikidata.org/wiki/Q7618701","display_name":"Stomatal conductance","level":3,"score":0.5281999707221985},{"id":"https://openalex.org/C183688256","wikidata":"https://www.wikidata.org/wiki/Q11982","display_name":"Photosynthesis","level":2,"score":0.492900013923645},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.4781000018119812},{"id":"https://openalex.org/C161221295","wikidata":"https://www.wikidata.org/wiki/Q185973","display_name":"Plant physiology","level":2,"score":0.42750000953674316},{"id":"https://openalex.org/C27406016","wikidata":"https://www.wikidata.org/wiki/Q17216","display_name":"Rootstock","level":2,"score":0.420199990272522},{"id":"https://openalex.org/C2781112554","wikidata":"https://www.wikidata.org/wiki/Q409202","display_name":"Bicarbonate","level":2,"score":0.39959999918937683},{"id":"https://openalex.org/C2775914622","wikidata":"https://www.wikidata.org/wiki/Q284089","display_name":"Carbonic anhydrase","level":3,"score":0.38760000467300415},{"id":"https://openalex.org/C75649859","wikidata":"https://www.wikidata.org/wiki/Q148877","display_name":"Assimilation (phonology)","level":2,"score":0.3781999945640564},{"id":"https://openalex.org/C3119751","wikidata":"https://www.wikidata.org/wiki/Q3935532","display_name":"Nitrogen assimilation","level":3,"score":0.32409998774528503},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31859999895095825},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.31439998745918274},{"id":"https://openalex.org/C138626823","wikidata":"https://www.wikidata.org/wiki/Q916504","display_name":"Chromosomal translocation","level":3,"score":0.2806999981403351},{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.2800999879837036},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2770000100135803},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.27309998869895935},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.2596000134944916},{"id":"https://openalex.org/C50212798","wikidata":"https://www.wikidata.org/wiki/Q422516","display_name":"RuBisCO","level":3,"score":0.25760000944137573},{"id":"https://openalex.org/C155868670","wikidata":"https://www.wikidata.org/wiki/Q534131","display_name":"Root system","level":2,"score":0.2574999928474426}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.21.759893","doi":"https://doi.org/10.64898/2026.10.21.759893","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.21.759893","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.759893.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.21.759893","doi":"https://doi.org/10.64898/2026.10.21.759893","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.21.759893","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/21/2026.10.21.759893.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.6875,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7223088694.pdf","grobid_xml":"https://content.openalex.org/works/W7223088694.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2297366033","https://openalex.org/W4362505683","https://openalex.org/W4379114584","https://openalex.org/W4392056205","https://openalex.org/W2729386344","https://openalex.org/W4296801290","https://openalex.org/W7127334338","https://openalex.org/W7213559273","https://openalex.org/W2394538839","https://openalex.org/W2748831343"],"abstract_inverted_index":{"Abstract":[0],"\u03b2-Carbonic":[1],"anhydrases":[2],"(\u03b2CAs)":[3],"catalyze":[4],"the":[5,23,123],"reversible":[6],"interconversion":[7],"of":[8,26],"CO":[9,131,144],"2":[10,132,145],"and":[11,13,19,42,60,71,95,102,134,186,197],"bicarbonate":[12],"contribute":[14],"to":[15,64,78,122,166,172,193],"plant":[16,154,194],"carbon":[17],"metabolism":[18],"environmental":[20,62],"responses.":[21],"However,":[22],"physiological":[24,179],"functions":[25],"individual":[27],"\u03b2CA":[28],"isoforms":[29],"in":[30,44,68,84,170,184],"crops":[31],"remain":[32],"poorly":[33],"understood.":[34],"Here,":[35],"we":[36],"investigated":[37],"two":[38],"chloroplast-localized":[39,182],"\u03b2CAs,":[40],"Sl\u03b2CA1":[41,89],"Sl\u03b2CA4,":[43],"tomato":[45,185],"(":[46],"Solanum":[47],"lycopersicum":[48],").":[49],"We":[50],"combined":[51],"subcellular":[52],"localization,":[53],"CRISPR/Cas9":[54],"mutagenesis,":[55],"grafting,":[56],"leaf":[57],"gas-exchange":[58],"measurements,":[59],"controlled":[61],"treatments":[63],"examine":[65],"their":[66,81],"roles":[67,180],"growth,":[69,115,195],"photosynthesis,":[70,96,196],"stomatal":[72,135,168,199],"developmental":[73,200],"plasticity.":[74,201],"Both":[75],"proteins":[76],"localized":[77],"chloroplasts":[79],"but":[80,150],"disruption":[82],"resulted":[83],"markedly":[85],"different":[86],"phenotypes.":[87],"While":[88],"mutants":[90,98,127,164],"showed":[91,129],"largely":[92,141],"normal":[93],"growth":[94,104,124,149],"Sl\u03b2CA4":[97,126,163,188],"exhibited":[99],"severe":[100],"root":[101,119,157],"shoot":[103],"defects.":[105,125],"Grafting":[106],"mutant":[107],"shoots":[108],"onto":[109],"wild-type":[110,161],"rootstocks":[111],"partially":[112,146],"restored":[113],"vegetative":[114],"indicating":[116],"that":[117],"impaired":[118],"function":[120],"contributes":[121],"also":[128],"reduced":[130],"assimilation":[133],"conductance,":[136],"while":[137],"electron":[138],"transport":[139],"remained":[140],"unaffected.":[142],"Elevated":[143],"improved":[147],"leaflet":[148],"did":[151],"not":[152],"restore":[153],"height":[155],"or":[156],"elongation.":[158],"Furthermore,":[159],"unlike":[160],"plants,":[162],"failed":[165],"increase":[167],"index":[169],"response":[171],"elevated":[173],"temperature.":[174],"These":[175],"findings":[176],"reveal":[177],"distinct":[178],"for":[181],"\u03b2CAs":[183],"identify":[187],"as":[189],"an":[190],"important":[191],"contributor":[192],"temperature-dependent":[198]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-22T00:00:00"}
