{"id":"https://openalex.org/W7222715985","doi":"https://doi.org/10.3390/pathogens15111161","title":"Pilot Production and Intralaboratory Characterization of a Candidate Genomic DNA Reference Material from Mycobacterium bovis for PCR-Based Molecular Assays","display_name":"Pilot Production and Intralaboratory Characterization of a Candidate Genomic DNA Reference Material from Mycobacterium bovis for PCR-Based Molecular Assays","publication_year":2026,"publication_date":"2026-11-06","ids":{"openalex":"https://openalex.org/W7222715985","doi":"https://doi.org/10.3390/pathogens15111161"},"language":"en","primary_location":{"id":"doi:10.3390/pathogens15111161","doi":"https://doi.org/10.3390/pathogens15111161","is_oa":true,"landing_page_url":"https://doi.org/10.3390/pathogens15111161","pdf_url":null,"source":{"id":"https://openalex.org/S4210239906","display_name":"Pathogens","issn_l":"2076-0817","issn":["2076-0817"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathogens","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3390/pathogens15111161","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091973394","display_name":"Camila Lota","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Camila Lota","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158933160","display_name":"Sergio D\u00e1vila","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sergio D\u00e1vila","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158920525","display_name":"John E. Leguizam\u00f3n","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John E. Leguizam\u00f3n","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5158886459","display_name":"Carlos Y. Soto","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Carlos Y. Soto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5158886459"],"corresponding_institution_ids":[],"apc_list":{"value":2200,"currency":"CHF","value_usd":2654},"apc_paid":{"value":2200,"currency":"CHF","value_usd":2654},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"15","issue":"11","first_page":"1161","last_page":"1161"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11890","display_name":"Scientific Measurement and Uncertainty Evaluation","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/3105","display_name":"Instrumentation"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11890","display_name":"Scientific Measurement and Uncertainty Evaluation","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/3105","display_name":"Instrumentation"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.006500000134110451,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycobacterium-bovis","display_name":"Mycobacterium bovis","score":0.9909999966621399},{"id":"https://openalex.org/keywords/quality-control-materials","display_name":"quality control materials","score":0.7129999995231628},{"id":"https://openalex.org/keywords/pcr-detection","display_name":"PCR detection","score":0.7480000257492065},{"id":"https://openalex.org/keywords/droplet-digital-pcr","display_name":"droplet digital PCR","score":0.9120000004768372},{"id":"https://openalex.org/keywords/qpcr","display_name":"qPCR","score":0.746999979019165},{"id":"https://openalex.org/keywords/copy-number","display_name":"copy number","score":0.45500001311302185},{"id":"https://openalex.org/keywords/dna-extraction","display_name":"DNA extraction","score":0.6000000238418579},{"id":"https://openalex.org/keywords/stability","display_name":"stability","score":0.3310000002384186},{"id":"https://openalex.org/keywords/bovine-tuberculosis","display_name":"bovine tuberculosis","score":0.6179999709129333}],"concepts":[{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.7353000044822693},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5562000274658203},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.5169000029563904},{"id":"https://openalex.org/C2778497218","wikidata":"https://www.wikidata.org/wiki/Q133447","display_name":"Mycobacterium bovis","level":4,"score":0.5148000121116638},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.48100000619888306},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.47920000553131104},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40959998965263367},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4056999981403351},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3846000134944916},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.359499990940094},{"id":"https://openalex.org/C3020001037","wikidata":"https://www.wikidata.org/wiki/Q836575","display_name":"Quality assessment","level":3,"score":0.3472000062465668},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34529998898506165},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3384000062942505},{"id":"https://openalex.org/C79386861","wikidata":"https://www.wikidata.org/wiki/Q476697","display_name":"DNA profiling","level":3,"score":0.3312000036239624},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.3109999895095825},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.3052000105381012},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2939999997615814},{"id":"https://openalex.org/C137209882","wikidata":"https://www.wikidata.org/wiki/Q1403517","display_name":"Measurement uncertainty","level":2,"score":0.28369998931884766},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.2793999910354614},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.2728999853134155},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.2547999918460846},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.25369998812675476}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.3390/pathogens15111161","doi":"https://doi.org/10.3390/pathogens15111161","is_oa":true,"landing_page_url":"https://doi.org/10.3390/pathogens15111161","pdf_url":null,"source":{"id":"https://openalex.org/S4210239906","display_name":"Pathogens","issn_l":"2076-0817","issn":["2076-0817"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathogens","raw_type":"journal-article"},{"id":"doi:10.20944/preprints202610.1594.v1","doi":"https://doi.org/10.20944/preprints202610.1594.v1","is_oa":true,"landing_page_url":"https://doi.org/10.20944/preprints202610.1594.v1","pdf_url":null,"source":{"id":"https://openalex.org/S6309402219","display_name":"Preprints.org","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.3390/pathogens15111161","doi":"https://doi.org/10.3390/pathogens15111161","is_oa":true,"landing_page_url":"https://doi.org/10.3390/pathogens15111161","pdf_url":null,"source":{"id":"https://openalex.org/S4210239906","display_name":"Pathogens","issn_l":"2076-0817","issn":["2076-0817"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathogens","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320328732","display_name":"Instituto Colombiano de Cr\u00e9dito Educativo y Estudios T\u00e9cnicos en el Exterior","ror":"https://ror.org/03jctmx41"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":52,"referenced_works":["https://openalex.org/W2065898689","https://openalex.org/W2096202905","https://openalex.org/W2149903385","https://openalex.org/W2553901927","https://openalex.org/W2608363029","https://openalex.org/W2724299949","https://openalex.org/W2804331934","https://openalex.org/W2918273319","https://openalex.org/W2947332671","https://openalex.org/W2970021049","https://openalex.org/W3021732351","https://openalex.org/W3036081023","https://openalex.org/W3036652145","https://openalex.org/W3084177816","https://openalex.org/W3112202094","https://openalex.org/W3160117284","https://openalex.org/W3185747323","https://openalex.org/W3200115972","https://openalex.org/W4213036240","https://openalex.org/W4213457615","https://openalex.org/W4220968819","https://openalex.org/W4306896958","https://openalex.org/W4311883848","https://openalex.org/W4377024432","https://openalex.org/W4383104961","https://openalex.org/W4384304909","https://openalex.org/W4385249294","https://openalex.org/W4386002849","https://openalex.org/W4386725596","https://openalex.org/W4386886916","https://openalex.org/W4390274612","https://openalex.org/W4391831317","https://openalex.org/W4392106421","https://openalex.org/W4392588251","https://openalex.org/W4393127071","https://openalex.org/W4393443844","https://openalex.org/W4396933133","https://openalex.org/W4398159278","https://openalex.org/W4399640836","https://openalex.org/W4399729226","https://openalex.org/W4403245797","https://openalex.org/W4403529578","https://openalex.org/W4404392446","https://openalex.org/W4404854262","https://openalex.org/W4405397738","https://openalex.org/W4405428672","https://openalex.org/W4405807079","https://openalex.org/W4407206133","https://openalex.org/W4409505417","https://openalex.org/W4409736934","https://openalex.org/W4416266441","https://openalex.org/W4416719045"],"related_works":["https://openalex.org/W2166203218","https://openalex.org/W4414779279","https://openalex.org/W2972230839","https://openalex.org/W2353844859","https://openalex.org/W4402090552","https://openalex.org/W2995722431","https://openalex.org/W2334193053","https://openalex.org/W2391318759","https://openalex.org/W4411308937","https://openalex.org/W2148010332"],"abstract_inverted_index":{"Accurate":[0],"and":[1,33,41,54,61,67,90,122,125,132,147,211,234],"reliable":[2],"PCR-based":[3,216],"detection":[4,98],"of":[5,88,92,99,120,130,145,195,215],"Mycobacterium":[6],"bovis":[7,30,221],"requires":[8],"quality-control":[9],"materials":[10],"with":[11,73,116],"well-defined":[12],"properties":[13],"to":[14,142,229],"support":[15,190],"molecular":[16,51],"measurements.":[17],"In":[18],"this":[19],"study,":[20],"a":[21,126,200],"candidate":[22,110,201],"genomic":[23,46,203,222],"DNA":[24,47,204],"reference":[25],"material":[26,71,164,178,198],"(RM)":[27],"from":[28],"M.":[29,220],"was":[31],"produced":[32],"characterized":[34],"under":[35,105,183],"intralaboratory":[36],"conditions.":[37,108,186],"An":[38],"optimized":[39],"extraction":[40],"pilot-production":[42],"workflow":[43],"yielded":[44],"high-molecular-weight":[45],"suitable":[48],"for":[49,70,78,154,202,206],"quantitative":[50],"applications.":[52],"qPCR":[53,82],"dPCR":[55,74,96],"measurement":[56,83,217],"procedures":[57,84,218],"targeting":[58],"the":[59,76,106,162,176,184,188,191,196],"Mb3145":[60],"CysA3":[62],"targets":[63],"were":[64,114],"analytically":[65],"evaluated":[66,107],"subsequently":[68],"used":[69],"characterization,":[72],"providing":[75],"measurements":[77],"copy-number":[79,118],"assignment.":[80],"The":[81],"showed":[85,165,179],"amplification":[86],"efficiencies":[87],"89\u2013103%":[89],"coefficients":[91],"determination":[93],"\u22650.98,":[94],"whereas":[95,175],"enabled":[97],"approximately":[100],"3":[101],"copies":[102],"per":[103],"reaction":[104],"Two":[109],"RM":[111,205],"concentration":[112,150],"levels":[113,151],"produced,":[115],"assigned":[117],"concentrations":[119],"62,922":[121],"426":[123],"copies/\u00b5L":[124],"relative":[127,141],"expanded":[128],"uncertainties":[129,144],"8.5%":[131],"15.9%":[133],"(k":[134],"=":[135],"2),":[136],"respectively.":[137],"Between-unit":[138],"homogeneity":[139],"contributed":[140],"standard":[143],"0.8%":[146],"1.2%.":[148],"Both":[149],"remained":[152],"stable":[153],"at":[155,159,172],"least":[156],"eight":[157],"weeks":[158],"4":[160,173],"\u00b0C;":[161],"high-concentration":[163],"no":[166],"significant":[167,180],"trend":[168],"over":[169],"12":[170],"months":[171],"\u00b0C,":[174],"low-concentration":[177],"long-term":[181],"change":[182],"same":[185],"Overall,":[187],"results":[189],"intended":[192],"analytical":[193,212],"use":[194],"pilot":[197],"as":[199],"quality":[207],"control,":[208],"method":[209],"verification,":[210],"performance":[213],"assessment":[214],"involving":[219],"DNA.":[223],"Further":[224],"interlaboratory":[225],"evaluation":[226],"is":[227],"required":[228],"establish":[230],"its":[231],"broader":[232],"applicability":[233],"comparability":[235],"across":[236],"laboratories.":[237]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-17T00:00:00"}
