{"id":"https://openalex.org/W7221901352","doi":"https://doi.org/10.1002/biot.70316","title":"An Integrated Transient Expression and UPLC\u2010PDA Approach for Functional Genomics and Metabolic Analysis of Cannabis sativa","display_name":"An Integrated Transient Expression and UPLC\u2010PDA Approach for Functional Genomics and Metabolic Analysis of Cannabis sativa","publication_year":2026,"publication_date":"2026-10-01","ids":{"openalex":"https://openalex.org/W7221901352","doi":"https://doi.org/10.1002/biot.70316","pmid":"https://pubmed.ncbi.nlm.nih.gov/42875966","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13669522"},"language":"en","primary_location":{"id":"doi:10.1002/biot.70316","doi":"https://doi.org/10.1002/biot.70316","is_oa":true,"landing_page_url":"https://doi.org/10.1002/biot.70316","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70316","source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70316","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001962266","display_name":"Sarah Pullano","orcid":"https://orcid.org/0009-0002-9854-0023","observed_orcids":["https://orcid.org/0009-0002-9854-0023"]},"institutions":[{"id":"https://openalex.org/I192455969","display_name":"York University","ror":"https://ror.org/05fq50484","country_code":"CA","type":"education","lineage":["https://openalex.org/I192455969"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Sarah Pullano","raw_affiliation_strings":["Department of Biology York University  Toronto Ontario Canada"],"raw_orcid":"https://orcid.org/0009-0002-9854-0023","affiliations":[{"raw_affiliation_string":"Department of Biology York University  Toronto Ontario Canada","institution_ids":["https://openalex.org/I192455969"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157699223","display_name":"Aida Shahabi","orcid":"https://orcid.org/0009-0002-2142-5385","observed_orcids":["https://orcid.org/0009-0002-2142-5385"]},"institutions":[{"id":"https://openalex.org/I192455969","display_name":"York University","ror":"https://ror.org/05fq50484","country_code":"CA","type":"education","lineage":["https://openalex.org/I192455969"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Aida Shahabi","raw_affiliation_strings":["Department of Biology York University  Toronto Ontario Canada"],"raw_orcid":"https://orcid.org/0009-0002-2142-5385","affiliations":[{"raw_affiliation_string":"Department of Biology York University  Toronto Ontario Canada","institution_ids":["https://openalex.org/I192455969"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157701590","display_name":"Parmis Shariat Torbaghani","orcid":"https://orcid.org/0009-0008-8462-7842","observed_orcids":["https://orcid.org/0009-0008-8462-7842"]},"institutions":[{"id":"https://openalex.org/I192455969","display_name":"York University","ror":"https://ror.org/05fq50484","country_code":"CA","type":"education","lineage":["https://openalex.org/I192455969"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Parmis Shariat Torbaghani","raw_affiliation_strings":["Department of Biology York University  Toronto Ontario Canada"],"raw_orcid":"https://orcid.org/0009-0008-8462-7842","affiliations":[{"raw_affiliation_string":"Department of Biology York University  Toronto Ontario Canada","institution_ids":["https://openalex.org/I192455969"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103167795","display_name":"Ivan\u00a0M. Monsalvo","orcid":"https://orcid.org/0009-0003-9709-0830","observed_orcids":["https://orcid.org/0009-0003-9709-0830"]},"institutions":[{"id":"https://openalex.org/I192455969","display_name":"York University","ror":"https://ror.org/05fq50484","country_code":"CA","type":"education","lineage":["https://openalex.org/I192455969"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Ivan Monsalvo","raw_affiliation_strings":["Department of Biology York University  Toronto Ontario Canada"],"raw_orcid":"https://orcid.org/0009-0003-9709-0830","affiliations":[{"raw_affiliation_string":"Department of Biology York University  Toronto Ontario Canada","institution_ids":["https://openalex.org/I192455969"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158820181","display_name":"Madison Sandler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I192455969","display_name":"York University","ror":"https://ror.org/05fq50484","country_code":"CA","type":"education","lineage":["https://openalex.org/I192455969"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Madison Sandler","raw_affiliation_strings":["Department of Biology York University  Toronto Ontario Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology York University  Toronto Ontario Canada","institution_ids":["https://openalex.org/I192455969"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022830159","display_name":"Adam Sumner","orcid":"https://orcid.org/0009-0007-4745-3305","observed_orcids":["https://orcid.org/0009-0007-4745-3305"]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adam Sumner","raw_affiliation_strings":["College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"],"raw_orcid":"https://orcid.org/0009-0007-4745-3305","affiliations":[{"raw_affiliation_string":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102929107","display_name":"Alex Harris","orcid":"https://orcid.org/0000-0002-3120-655X","observed_orcids":["https://orcid.org/0000-0002-3120-655X","https://orcid.org/0000-0002-5764-7513"]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alex Harris","raw_affiliation_strings":["College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"],"raw_orcid":"https://orcid.org/0000-0002-3120-655X","affiliations":[{"raw_affiliation_string":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029471060","display_name":"David C. Haak","orcid":"https://orcid.org/0000-0002-3692-3152","observed_orcids":["https://orcid.org/0000-0002-3692-3152"]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David Haak","raw_affiliation_strings":["College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"],"raw_orcid":"https://orcid.org/0000-0002-3692-3152","affiliations":[{"raw_affiliation_string":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068145426","display_name":"Bastiaan O. R. Bargmann","orcid":"https://orcid.org/0000-0001-8406-5036","observed_orcids":["https://orcid.org/0000-0001-8406-5036"]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bastiaan Bargmann","raw_affiliation_strings":["College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA"],"raw_orcid":"https://orcid.org/0000-0001-8406-5036","affiliations":[{"raw_affiliation_string":"College of Agricultural and Life Sciences Virginia Tech  Blacksburg Virginia USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046078073","display_name":"Nik Kovinich","orcid":"https://orcid.org/0000-0002-9396-6626","observed_orcids":["https://orcid.org/0000-0002-9396-6626"]},"institutions":[{"id":"https://openalex.org/I192455969","display_name":"York University","ror":"https://ror.org/05fq50484","country_code":"CA","type":"education","lineage":["https://openalex.org/I192455969"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Nik Kovinich","raw_affiliation_strings":["Department of Biology York University  Toronto Ontario Canada"],"raw_orcid":"https://orcid.org/0000-0002-9396-6626","affiliations":[{"raw_affiliation_string":"Department of Biology York University  Toronto Ontario Canada","institution_ids":["https://openalex.org/I192455969"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5046078073"],"corresponding_institution_ids":["https://openalex.org/I192455969"],"apc_list":{"value":4780,"currency":"USD","value_usd":4780},"apc_paid":{"value":4780,"currency":"USD","value_usd":4780},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.74667152,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"10","first_page":"e70316","last_page":"e70316"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.7006999850273132,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.7006999850273132,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.28760001063346863,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cannabis-sativa","display_name":"Cannabis sativa","score":1.0},{"id":"https://openalex.org/keywords/transient-expression","display_name":"transient expression","score":0.9440000057220459},{"id":"https://openalex.org/keywords/transformation-efficiency","display_name":"transformation efficiency","score":0.8989999890327454},{"id":"https://openalex.org/keywords/p35s-promoter","display_name":"p35S promoter","score":0.7350000143051147},{"id":"https://openalex.org/keywords/cannabinoids","display_name":"cannabinoids","score":0.7310000061988831},{"id":"https://openalex.org/keywords/uplc-pda","display_name":"UPLC-PDA","score":0.8709999918937683},{"id":"https://openalex.org/keywords/metabolic-profiling","display_name":"metabolite profiling","score":0.8479999899864197},{"id":"https://openalex.org/keywords/functional-genomics","display_name":"functional genomics","score":0.7450000047683716},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.9300000071525574},{"id":"https://openalex.org/keywords/leaf-discs","display_name":"leaf discs","score":0.4699999988079071},{"id":"https://openalex.org/keywords/seedlings","display_name":"seedlings","score":0.9039999842643738}],"concepts":[{"id":"https://openalex.org/C161078062","wikidata":"https://www.wikidata.org/wiki/Q1068690","display_name":"Functional genomics","level":5,"score":0.8253999948501587},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6851000189781189},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6492000222206116},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.6399999856948853},{"id":"https://openalex.org/C2780012829","wikidata":"https://www.wikidata.org/wiki/Q2827241","display_name":"Agroinfiltration","level":4,"score":0.542900025844574},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.5062000155448914},{"id":"https://openalex.org/C2992191599","wikidata":"https://www.wikidata.org/wiki/Q26726","display_name":"Cannabis sativa","level":2,"score":0.46650001406669617},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.44830000400543213},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.41370001435279846},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.3986000120639801},{"id":"https://openalex.org/C55112680","wikidata":"https://www.wikidata.org/wiki/Q190549","display_name":"Functional analysis","level":3,"score":0.397599995136261},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.3930000066757202},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.3698999881744385},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3662000000476837},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.366100013256073},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.3653999865055084},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3625999987125397},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.3440999984741211},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.32850000262260437},{"id":"https://openalex.org/C25365240","wikidata":"https://www.wikidata.org/wiki/Q1897151","display_name":"Reverse genetics","level":4,"score":0.3237999975681305},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.32260000705718994},{"id":"https://openalex.org/C178050479","wikidata":"https://www.wikidata.org/wiki/Q7834084","display_name":"Transformation efficiency","level":5,"score":0.3176000118255615},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.3075999915599823},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2980000078678131},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.2791000008583069},{"id":"https://openalex.org/C97037327","wikidata":"https://www.wikidata.org/wiki/Q5334291","display_name":"Ectopic expression","level":3,"score":0.2734000086784363},{"id":"https://openalex.org/C100012643","wikidata":"https://www.wikidata.org/wiki/Q1384844","display_name":"Expression cassette","level":5,"score":0.26030001044273376}],"mesh":[{"descriptor_ui":"D002186","descriptor_name":"Cannabinoids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002186","descriptor_name":"Cannabinoids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002188","descriptor_name":"Cannabis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002188","descriptor_name":"Cannabis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014170","descriptor_name":"Transformation, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D060054","descriptor_name":"Agrobacterium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/biot.70316","doi":"https://doi.org/10.1002/biot.70316","is_oa":true,"landing_page_url":"https://doi.org/10.1002/biot.70316","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70316","source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13669522","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13669522/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13669522/pdf/BIOT-21-e70316.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnol J","raw_type":"Text"},{"id":"pmid:42875966","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42875966","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/biot.70316","doi":"https://doi.org/10.1002/biot.70316","is_oa":true,"landing_page_url":"https://doi.org/10.1002/biot.70316","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70316","source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.40450000762939453}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5599141082","display_name":null,"funder_award_id":"596223","funder_id":"https://openalex.org/F4320334593","funder_display_name":"Natural Sciences and Engineering Research Council of Canada"},{"id":"https://openalex.org/G9001302872","display_name":null,"funder_award_id":"RGPIN\u20102025\u201006282","funder_id":"https://openalex.org/F4320334593","funder_display_name":"Natural Sciences and Engineering Research Council of Canada"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320332299","display_name":"National Institute of Food and Agriculture","ror":"https://ror.org/05qx3fv49"},{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7221901352.pdf","grobid_xml":"https://content.openalex.org/works/W7221901352.grobid-xml"},"referenced_works_count":33,"referenced_works":["https://openalex.org/W1980559237","https://openalex.org/W1990418094","https://openalex.org/W2022441904","https://openalex.org/W2029490085","https://openalex.org/W2083617349","https://openalex.org/W2109902472","https://openalex.org/W2120048927","https://openalex.org/W2463273054","https://openalex.org/W2566918653","https://openalex.org/W2605224985","https://openalex.org/W2736503201","https://openalex.org/W2795577939","https://openalex.org/W2888625870","https://openalex.org/W3006695265","https://openalex.org/W3035998300","https://openalex.org/W3091935517","https://openalex.org/W3115804709","https://openalex.org/W3159896158","https://openalex.org/W4200416248","https://openalex.org/W4251681721","https://openalex.org/W4281739725","https://openalex.org/W4318052281","https://openalex.org/W4385244891","https://openalex.org/W4392137357","https://openalex.org/W4399034059","https://openalex.org/W4399240159","https://openalex.org/W4399363648","https://openalex.org/W4400930119","https://openalex.org/W4405548123","https://openalex.org/W4411338329","https://openalex.org/W4411524027","https://openalex.org/W7154689987","https://openalex.org/W7162422172"],"related_works":["https://openalex.org/W4399363648","https://openalex.org/W3035998300","https://openalex.org/W4392240458","https://openalex.org/W3011690174","https://openalex.org/W4410374445","https://openalex.org/W3134714946","https://openalex.org/W4395078161","https://openalex.org/W2036128681","https://openalex.org/W3115804709","https://openalex.org/W2620091023"],"abstract_inverted_index":{"Cannabis":[0,61],"sativa":[1],"(Cannabis)":[2],"is":[3,22],"a":[4,129,185],"high-value":[5],"medicinal":[6],"crop":[7],"whose":[8],"functional":[9,162],"genomics":[10,163],"and":[11,29,53,81,91,99,115,135,139,154,164,194],"metabolic":[12,165],"engineering":[13,166,197],"remain":[14],"constrained":[15],"by":[16,104],"limited":[17],"genetic":[18,125],"tools.":[19],"The":[20],"species":[21],"regarded":[23],"as":[24],"recalcitrant":[25],"to":[26,40,75,144],"stable":[27],"transformation,":[28],"existing":[30],"transient":[31,55,177],"expression":[32,57,101,179],"systems":[33],"exhibit":[34],"low":[35],"efficiency":[36,98,114],"or":[37],"are":[38,102],"restricted":[39],"hemp":[41],"cultivars.":[42],"Here,":[43],"we":[44,69,94,127],"report":[45],"two":[46],"Agrobacterium-mediated":[47],"agroinfiltration":[48],"protocols":[49],"enabling":[50,132],"rapid,":[51],"robust,":[52],"reproducible":[54],"gene":[56,178,189],"in":[58,77,83,198],"drug-type,":[59],"high-THC":[60],"tissues.":[62],"Using":[63],"the":[64,111,119,149],"RUBY":[65],"betalain":[66],"reporter":[67,121],"system,":[68],"demonstrate":[70,95],"transformation":[71,97,113],"efficiencies":[72],"of":[73,137],"up":[74],"100%":[76],"mature":[78],"leaf":[79],"disks":[80],"81%-100%":[82],"whole":[84],"seedlings.":[85],"By":[86],"independently":[87],"comparing":[88],"Agrobacterium":[89],"strains":[90],"vector":[92],"configurations,":[93],"that":[96],"transgene":[100],"influenced":[103],"distinct":[105],"experimental":[106],"variables,":[107],"with":[108,180],"EHA105":[109],"providing":[110,184],"highest":[112],"p35S-based":[116],"vectors":[117],"producing":[118],"strongest":[120],"expression.":[122],"Complementing":[123],"these":[124,169],"tools,":[126],"developed":[128],"UPLC-PDA":[130],"method":[131,150],"simultaneous":[133],"detection":[134],"quantification":[136],"cannabinoids":[138],"cannflavins.":[140],"While":[141],"not":[142],"intended":[143],"replace":[145],"mass":[146],"spectrometry-based":[147],"analyses,":[148],"provides":[151],"an":[152,172],"accessible":[153],"cost-effective":[155],"platform":[156],"for":[157,188],"comparative":[158],"metabolite":[159],"profiling":[160],"during":[161],"studies.":[167],"Together,":[168],"advances":[170],"establish":[171],"integrated":[173],"workflow":[174],"linking":[175],"high-efficiency":[176],"streamlined":[181],"biochemical":[182],"analysis,":[183],"practical":[186],"foundation":[187],"function":[190],"studies,":[191],"pathway":[192],"interrogation,":[193],"biotechnology-driven":[195],"trait":[196],"Cannabis.":[199]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-10-16T00:00:00"}