{"id":"https://openalex.org/W7221879310","doi":"https://doi.org/10.64898/2026.10.08.757701","title":"Cholesterol constrains pore formation by mammalian gasdermins","display_name":"Cholesterol constrains pore formation by mammalian gasdermins","publication_year":2026,"publication_date":"2026-10-15","ids":{"openalex":"https://openalex.org/W7221879310","doi":"https://doi.org/10.64898/2026.10.08.757701"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.08.757701","doi":"https://doi.org/10.64898/2026.10.08.757701","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.08.757701","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/15/2026.10.08.757701.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/15/2026.10.08.757701.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060749891","display_name":"Yisha Liang","orcid":"https://orcid.org/0000-0001-8409-2368","observed_orcids":["https://orcid.org/0000-0001-8409-2368"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yisha Liang","raw_affiliation_strings":["Harvard Medical School, Boston, MA 02115, USA;","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0001-8409-2368","affiliations":[{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059364365","display_name":"Isabella Rose Fraschilla","orcid":"https://orcid.org/0000-0002-4166-6293","observed_orcids":["https://orcid.org/0000-0002-4166-6293"]},"institutions":[{"id":"https://openalex.org/I4390039253","display_name":"Koch Institute for Integrative Cancer Research At MIT","ror":"https://ror.org/01xd6q208","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039253","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Isabella R. Evavold","raw_affiliation_strings":["Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;","Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I4390039253","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034395409","display_name":"Sepideh Parvanian","orcid":"https://orcid.org/0000-0002-3884-9961","observed_orcids":["https://orcid.org/0000-0002-3884-9961"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I4210155419","display_name":"Center for Systems Biology","ror":"https://ror.org/05r3dyn47","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210087915","https://openalex.org/I4210155419","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sepideh Parvanian","raw_affiliation_strings":["Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","Department of Pathology, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA","Harvard Medical School, Boston, MA 02115, USA;"],"raw_orcid":"https://orcid.org/0000-0002-3884-9961","affiliations":[{"raw_affiliation_string":"Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","institution_ids":["https://openalex.org/I4210087915","https://openalex.org/I4210155419"]},{"raw_affiliation_string":"Department of Pathology, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA","institution_ids":["https://openalex.org/I4210087915"]},{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014666367","display_name":"Makiko Hayashi","orcid":"https://orcid.org/0000-0002-9189-0058","observed_orcids":["https://orcid.org/0000-0002-9189-0058"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I4210155419","display_name":"Center for Systems Biology","ror":"https://ror.org/05r3dyn47","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210087915","https://openalex.org/I4210155419","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Makiko Hayashi","raw_affiliation_strings":["Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","Department of Pathology, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA","Harvard Medical School, Boston, MA 02115, USA;","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0002-9189-0058","affiliations":[{"raw_affiliation_string":"Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","institution_ids":["https://openalex.org/I4210087915","https://openalex.org/I4210155419"]},{"raw_affiliation_string":"Department of Pathology, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA","institution_ids":["https://openalex.org/I4210087915"]},{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006710319","display_name":"Emerson Glassey","orcid":"https://orcid.org/0000-0002-7603-8203","observed_orcids":["https://orcid.org/0000-0002-7603-8203"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Emerson Glassey","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA 02115;","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA 02115;","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035636554","display_name":"Gema Gonz\u00e1lez-Rubio","orcid":"https://orcid.org/0000-0002-0204-482X","observed_orcids":["https://orcid.org/0000-0002-0204-482X"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gema Gonz\u00e1lez-Rubio","raw_affiliation_strings":["Harvard Medical School, Boston, MA 02115, USA;","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0002-0204-482X","affiliations":[{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114762869","display_name":"Inga M. Spannaus","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Inga M. Spannaus","raw_affiliation_strings":["Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070926471","display_name":"Victoria Andrade","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Victoria De Andrade","raw_affiliation_strings":["Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100019759","display_name":"Benjamin L. Allsup","orcid":"https://orcid.org/0000-0003-3873-1813","observed_orcids":["https://orcid.org/0000-0003-3873-1813"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benjamin L. Allsup","raw_affiliation_strings":["Department of Biological Engineering, MIT, Cambridge 02139, USA","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0003-3873-1813","affiliations":[{"raw_affiliation_string":"Department of Biological Engineering, MIT, Cambridge 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5137264375","display_name":"Fan Fei","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I4210155419","display_name":"Center for Systems Biology","ror":"https://ror.org/05r3dyn47","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210087915","https://openalex.org/I4210155419","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fan Fei","raw_affiliation_strings":["Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","Harvard Medical School, Boston, MA 02115, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","institution_ids":["https://openalex.org/I4210087915","https://openalex.org/I4210155419"]},{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063430862","display_name":"Bryan D. Bryson","orcid":"https://orcid.org/0000-0003-1716-6712","observed_orcids":["https://orcid.org/0000-0003-1716-6712"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bryan D. Bryson","raw_affiliation_strings":["Department of Biological Engineering, MIT, Cambridge 02139, USA","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0003-1716-6712","affiliations":[{"raw_affiliation_string":"Department of Biological Engineering, MIT, Cambridge 02139, USA","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041178880","display_name":"Aaron G. Schmidt","orcid":"https://orcid.org/0000-0003-3627-2553","observed_orcids":["https://orcid.org/0000-0003-3627-2553"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aaron G. Schmidt","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA 02115;","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0003-3627-2553","affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA 02115;","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071936673","display_name":"Tyler Jacks","orcid":"https://orcid.org/0000-0001-5785-8911","observed_orcids":["https://orcid.org/0000-0001-5785-8911"]},"institutions":[{"id":"https://openalex.org/I4390039253","display_name":"Koch Institute for Integrative Cancer Research At MIT","ror":"https://ror.org/01xd6q208","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039253","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tyler E. Jacks","raw_affiliation_strings":["Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;","Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0001-5785-8911","affiliations":[{"raw_affiliation_string":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I4390039253","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090885075","display_name":"Christopher Garris","orcid":"https://orcid.org/0000-0002-5764-6203","observed_orcids":["https://orcid.org/0000-0002-5764-6203"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I4210155419","display_name":"Center for Systems Biology","ror":"https://ror.org/05r3dyn47","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210087915","https://openalex.org/I4210155419","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher S. Garris","raw_affiliation_strings":["Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","Department of Pathology, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA","Harvard Medical School, Boston, MA 02115, USA;"],"raw_orcid":"https://orcid.org/0000-0002-5764-6203","affiliations":[{"raw_affiliation_string":"Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA;","institution_ids":["https://openalex.org/I4210087915","https://openalex.org/I4210155419"]},{"raw_affiliation_string":"Department of Pathology, Massachusetts General Hospital, 55 Fruit St, Boston, MA 02114, USA","institution_ids":["https://openalex.org/I4210087915"]},{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055933988","display_name":"Charles Lee Evavold","orcid":"https://orcid.org/0000-0003-3913-5626","observed_orcids":["https://orcid.org/0000-0003-3913-5626"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2802422659","display_name":"Ragon Institute of MGH, MIT and Harvard","ror":"https://ror.org/053r20n13","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I48633490","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Charles L. Evavold","raw_affiliation_strings":["Harvard Medical School, Boston, MA 02115, USA;","Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;"],"raw_orcid":"https://orcid.org/0000-0003-3913-5626","affiliations":[{"raw_affiliation_string":"Harvard Medical School, Boston, MA 02115, USA;","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Ragon Institute of Mass General, MIT, and Harvard, 600 Main Street, Cambridge, MA 02139, USA;","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5055933988"],"corresponding_institution_ids":["https://openalex.org/I136199984","https://openalex.org/I2802422659","https://openalex.org/I4210087915","https://openalex.org/I63966007"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11078","display_name":"Inflammasome and immune disorders","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11078","display_name":"Inflammasome and immune disorders","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gasdermin-d","display_name":"gasdermin D","score":0.7639999985694885},{"id":"https://openalex.org/keywords/gasdermin-e","display_name":"gasdermin E","score":0.8740000128746033},{"id":"https://openalex.org/keywords/pyroptosis","display_name":"pyroptosis","score":0.9909999966621399},{"id":"https://openalex.org/keywords/membrane-cholesterol","display_name":"membrane cholesterol","score":0.7419999837875366},{"id":"https://openalex.org/keywords/pore-formation","display_name":"pore formation","score":0.7940000295639038},{"id":"https://openalex.org/keywords/macrophages","display_name":"macrophages","score":0.7310000061988831},{"id":"https://openalex.org/keywords/cholesterol-depletion","display_name":"cholesterol depletion","score":0.5979999899864197},{"id":"https://openalex.org/keywords/cancer-immunotherapy","display_name":"cancer immunotherapy","score":0.6290000081062317},{"id":"https://openalex.org/keywords/chemoresistance","display_name":"chemoresistance","score":0.6620000004768372},{"id":"https://openalex.org/keywords/synthetic-mrna","display_name":"synthetic mRNA","score":0.5149999856948853},{"id":"https://openalex.org/keywords/crispr-screening","display_name":"CRISPR screen","score":0.628000020980835}],"concepts":[{"id":"https://openalex.org/C17172800","wikidata":"https://www.wikidata.org/wiki/Q7263707","display_name":"Pyroptosis","level":4,"score":0.8026999831199646},{"id":"https://openalex.org/C182615771","wikidata":"https://www.wikidata.org/wiki/Q1369386","display_name":"Lytic cycle","level":3,"score":0.7056999802589417},{"id":"https://openalex.org/C102747710","wikidata":"https://www.wikidata.org/wiki/Q824237","display_name":"Endosome","level":3,"score":0.6047000288963318},{"id":"https://openalex.org/C2778163477","wikidata":"https://www.wikidata.org/wiki/Q43656","display_name":"Cholesterol","level":2,"score":0.6043999791145325},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.5960000157356262},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5910999774932861},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.5773000121116638},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.557699978351593},{"id":"https://openalex.org/C79266657","wikidata":"https://www.wikidata.org/wiki/Q424178","display_name":"Lipid raft","level":3,"score":0.4372999966144562},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4359000027179718},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.4196999967098236},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.4129999876022339},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.3336000144481659},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.3294000029563904},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3221000134944916},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.3095000088214874},{"id":"https://openalex.org/C179185449","wikidata":"https://www.wikidata.org/wiki/Q219699","display_name":"Suppressor","level":3,"score":0.3086000084877014},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.2976999878883362},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2824000120162964},{"id":"https://openalex.org/C2778718757","wikidata":"https://www.wikidata.org/wiki/Q143623","display_name":"Sterol","level":3,"score":0.26759999990463257},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.2502000033855438}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.08.757701","doi":"https://doi.org/10.64898/2026.10.08.757701","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.08.757701","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/15/2026.10.08.757701.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.08.757701","doi":"https://doi.org/10.64898/2026.10.08.757701","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.08.757701","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/15/2026.10.08.757701.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3044218864","display_name":"Membrane Cholesterol as a Metabolic Checkpoint in Macrophage Pyroptosis","funder_award_id":"26POST1563608","funder_id":"https://openalex.org/F4320306230","funder_display_name":"American Heart Association"}],"funders":[{"id":"https://openalex.org/F4320306230","display_name":"American Heart Association","ror":"https://ror.org/013kjyp64"},{"id":"https://openalex.org/F4320306271","display_name":"Damon Runyon Cancer Research Foundation","ror":"https://ror.org/01gd7b947"},{"id":"https://openalex.org/F4320310214","display_name":"Fundaci\u00f3n Ram\u00f3n Areces","ror":"https://ror.org/04ra91131"},{"id":"https://openalex.org/F4320311529","display_name":"Bladder Cancer Advocacy Network","ror":"https://ror.org/04p3pep55"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7221879310.pdf","grobid_xml":"https://content.openalex.org/works/W7221879310.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7171546000","https://openalex.org/W4319433406","https://openalex.org/W3205526883","https://openalex.org/W7167919004","https://openalex.org/W4387938761","https://openalex.org/W4313373111","https://openalex.org/W2478343110","https://openalex.org/W2461915818","https://openalex.org/W3199367313","https://openalex.org/W4308955249"],"abstract_inverted_index":{"Abstract":[0],"Pyroptosis":[1],"is":[2,60],"a":[3,14,35,96],"lytic":[4,52],"form":[5],"of":[6,38,65,86,99],"cell":[7,139],"death":[8,140],"executed":[9],"by":[10,118],"gasdermin":[11,23,101],"pores.":[12],"Through":[13],"CRISPR":[15],"screen":[16],"to":[17,94,114],"uncover":[18],"macrophage":[19],"genes":[20],"required":[21],"for":[22,136,147],"D":[24],"(GSDMD)":[25],"pore":[26,39,90],"activity,":[27],"we":[28],"identify":[29],"the":[30,68,100,142],"endosome":[31],"adaptor":[32],"Rabgef1":[33,41,58],"as":[34,132],"positive":[36],"regulator":[37,98],"formation.":[40],"deficiency":[42,59],"preserves":[43],"NT-GSDMD":[44,49],"membrane":[45,87,129],"binding":[46],"but":[47],"prevents":[48],"oligomerization":[50,80],"and":[51,81,89,107,123,150],"death.":[53],"Mechanistic":[54],"analyses":[55],"revealed":[56],"that":[57],"associated":[61],"with":[62,145],"an":[63,133],"overabundance":[64],"cholesterol":[66,88,109,130],"in":[67],"plasma":[69],"membrane.":[70],"Cholesterol":[71],"depletion":[72],"or":[73],"redistribution":[74],"through":[75],"multiple":[76],"approaches":[77],"restores":[78],"GSDMD":[79],"pyroptosis.":[82],"The":[83],"Rabgef1-mediated":[84],"control":[85],"formation":[91],"was":[92],"found":[93],"be":[95],"general":[97],"family.":[102],"In":[103],"cancer":[104,151],"cells,":[105],"organoids,":[106],"tumors,":[108],"reduction":[110],"sensitizes":[111],"resistant":[112],"cells":[113],"GSDME":[115],"pyroptosis":[116],"driven":[117],"synthetic":[119],"mRNAs,":[120],"chemogenetic":[121],"induction,":[122],"chemotherapy":[124],"treatment.":[125],"These":[126],"results":[127],"establish":[128],"content":[131],"endogenous":[134],"checkpoint":[135],"gasdermin-mediated":[137],"pyroptotic":[138],"during":[141],"execution":[143],"phase,":[144],"implications":[146],"metabolic":[148],"disease":[149],"immunotherapy.":[152]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-16T00:00:00"}
