{"id":"https://openalex.org/W7221549526","doi":"https://doi.org/10.3390/plants15203103","title":"Development of an Efficient Agrobacterium-Mediated Transformation Protocol for the Tetraploid Medicinal and Vegetable Species Solanum villosum Mill.","display_name":"Development of an Efficient Agrobacterium-Mediated Transformation Protocol for the Tetraploid Medicinal and Vegetable Species Solanum villosum Mill.","publication_year":2026,"publication_date":"2026-10-10","ids":{"openalex":"https://openalex.org/W7221549526","doi":"https://doi.org/10.3390/plants15203103","pmid":"https://pubmed.ncbi.nlm.nih.gov/42943058","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13708614"},"language":"en","primary_location":{"id":"doi:10.3390/plants15203103","doi":"https://doi.org/10.3390/plants15203103","is_oa":true,"landing_page_url":"https://doi.org/10.3390/plants15203103","pdf_url":null,"source":{"id":"https://openalex.org/S4210230202","display_name":"Plants","issn_l":"2223-7747","issn":["2223-7747"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plants","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3390/plants15203103","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064033704","display_name":"Chunlan Piao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1284762954","display_name":"Zhejiang A & F University","ror":"https://ror.org/02vj4rn06","country_code":"CN","type":"education","lineage":["https://openalex.org/I1284762954"]},{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chun-Lan Piao","raw_affiliation_strings":["Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China","institution_ids":["https://openalex.org/I1284762954","https://openalex.org/I4210151987"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103062455","display_name":"Min\u2010Long Cui","orcid":"https://orcid.org/0000-0003-2370-2227","observed_orcids":["https://orcid.org/0000-0003-2370-2227"]},"institutions":[{"id":"https://openalex.org/I1284762954","display_name":"Zhejiang A & F University","ror":"https://ror.org/02vj4rn06","country_code":"CN","type":"education","lineage":["https://openalex.org/I1284762954"]},{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Min-Long Cui","raw_affiliation_strings":["Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China","institution_ids":["https://openalex.org/I1284762954","https://openalex.org/I4210151987"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5103062455"],"corresponding_institution_ids":["https://openalex.org/I1284762954","https://openalex.org/I4210151987"],"apc_list":{"value":2700,"currency":"CHF","value_usd":3258},"apc_paid":{"value":2700,"currency":"CHF","value_usd":3258},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.74522298,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"15","issue":"20","first_page":"3103","last_page":"3103"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12828","display_name":"Plant Genetic and Mutation Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/solanum-villosum","display_name":"Solanum villosum","score":1.0},{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.9850000143051147},{"id":"https://openalex.org/keywords/tetraploid-plants","display_name":"tetraploid plants","score":0.8679999709129333},{"id":"https://openalex.org/keywords/leaf-explants","display_name":"leaf explants","score":0.7870000004768372},{"id":"https://openalex.org/keywords/adventitious-shoot-regeneration","display_name":"adventitious shoot regeneration","score":0.8560000061988831},{"id":"https://openalex.org/keywords/thidiazuron","display_name":"thidiazuron","score":0.875},{"id":"https://openalex.org/keywords/naphthaleneacetic-acid","display_name":"naphthaleneacetic acid","score":0.7760000228881836},{"id":"https://openalex.org/keywords/silver-nitrate","display_name":"silver nitrate","score":0.5979999899864197},{"id":"https://openalex.org/keywords/gfp-reporter","display_name":"GFP reporter","score":0.597000002861023},{"id":"https://openalex.org/keywords/neomycin-phosphotransferase-ii","display_name":"neomycin phosphotransferase II","score":0.8790000081062317},{"id":"https://openalex.org/keywords/transgene-integration","display_name":"transgene integration","score":0.8450000286102295},{"id":"https://openalex.org/keywords/medicinal-plants","display_name":"medicinal plants","score":0.7210000157356262},{"id":"https://openalex.org/keywords/vegetable-crops","display_name":"vegetable crops","score":0.7829999923706055},{"id":"https://openalex.org/keywords/plant-tissue-culture","display_name":"plant tissue culture","score":0.6119999885559082}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8366000056266785},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.8066999912261963},{"id":"https://openalex.org/C42411736","wikidata":"https://www.wikidata.org/wiki/Q1550327","display_name":"Selectable marker","level":4,"score":0.7346000075340271},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.7014999985694885},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5192999839782715},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.5121999979019165},{"id":"https://openalex.org/C2776162087","wikidata":"https://www.wikidata.org/wiki/Q131472","display_name":"Agrobacterium tumefaciens","level":4,"score":0.5006999969482422},{"id":"https://openalex.org/C2781083041","wikidata":"https://www.wikidata.org/wiki/Q146555","display_name":"Solanum","level":2,"score":0.5001999735832214},{"id":"https://openalex.org/C161078062","wikidata":"https://www.wikidata.org/wiki/Q1068690","display_name":"Functional genomics","level":5,"score":0.46059998869895935},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.42669999599456787},{"id":"https://openalex.org/C178050479","wikidata":"https://www.wikidata.org/wiki/Q7834084","display_name":"Transformation efficiency","level":5,"score":0.4194999933242798},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.39660000801086426},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.37279999256134033},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.3693999946117401},{"id":"https://openalex.org/C2779227213","wikidata":"https://www.wikidata.org/wiki/Q3954823","display_name":"Marker-assisted selection","level":4,"score":0.33719998598098755},{"id":"https://openalex.org/C2780822416","wikidata":"https://www.wikidata.org/wiki/Q1934105","display_name":"Micropropagation","level":4,"score":0.31679999828338623},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3084999918937683},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.2793000042438507},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.2791000008583069},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.274399995803833},{"id":"https://openalex.org/C169282265","wikidata":"https://www.wikidata.org/wiki/Q17145598","display_name":"Molecular breeding","level":3,"score":0.259799987077713}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3390/plants15203103","doi":"https://doi.org/10.3390/plants15203103","is_oa":true,"landing_page_url":"https://doi.org/10.3390/plants15203103","pdf_url":null,"source":{"id":"https://openalex.org/S4210230202","display_name":"Plants","issn_l":"2223-7747","issn":["2223-7747"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plants","raw_type":"journal-article"},{"id":"pmid:42943058","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42943058","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plants (Basel, Switzerland)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13708614","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13708614/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plants (Basel)","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:3a4e73298f794df19fec4885f83e555f","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/3a4e73298f794df19fec4885f83e555f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Plants, Vol 15, Iss 20, p 3103 (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/plants15203103","doi":"https://doi.org/10.3390/plants15203103","is_oa":true,"landing_page_url":"https://doi.org/10.3390/plants15203103","pdf_url":null,"source":{"id":"https://openalex.org/S4210230202","display_name":"Plants","issn_l":"2223-7747","issn":["2223-7747"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plants","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.9208999872207642,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G947574900","display_name":null,"funder_award_id":"2018FR004","funder_id":"https://openalex.org/F4320325403","funder_display_name":"Zhejiang A and F University"}],"funders":[{"id":"https://openalex.org/F4320325403","display_name":"Zhejiang A and F University","ror":"https://ror.org/02vj4rn06"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W1992716708","https://openalex.org/W1995030293","https://openalex.org/W1995859312","https://openalex.org/W2006238325","https://openalex.org/W2014966960","https://openalex.org/W2024556021","https://openalex.org/W2035631080","https://openalex.org/W2044186731","https://openalex.org/W2046451872","https://openalex.org/W2050780029","https://openalex.org/W2058161072","https://openalex.org/W2135599428","https://openalex.org/W2139342899","https://openalex.org/W2212687567","https://openalex.org/W2313440198","https://openalex.org/W2463273054","https://openalex.org/W2480943605","https://openalex.org/W2518225655","https://openalex.org/W2767724387","https://openalex.org/W2989015368","https://openalex.org/W3091894549","https://openalex.org/W3098270900","https://openalex.org/W3112213817","https://openalex.org/W3119952098","https://openalex.org/W3172041497","https://openalex.org/W4210449807","https://openalex.org/W4212983031","https://openalex.org/W4306247731","https://openalex.org/W4317436665","https://openalex.org/W4321352191","https://openalex.org/W4382242846","https://openalex.org/W4386970332","https://openalex.org/W4408245306","https://openalex.org/W4410716774","https://openalex.org/W4411498929","https://openalex.org/W4412841146","https://openalex.org/W4416528078"],"related_works":["https://openalex.org/W2467982296","https://openalex.org/W2092513450","https://openalex.org/W2001440674","https://openalex.org/W7171943833","https://openalex.org/W1965194930","https://openalex.org/W2122265866","https://openalex.org/W2085058865","https://openalex.org/W7017157890","https://openalex.org/W4323365057","https://openalex.org/W7163538553"],"abstract_inverted_index":{"Genetic":[0],"transformation":[1,44,59,135,180],"is":[2,17],"a":[3,18,49,146,187],"cornerstone":[4],"approach":[5],"for":[6,54,183,190],"functional":[7],"genomics":[8],"and":[9,29,57,66,81,118,139,149,161,167,179,196],"molecular":[10,159],"breeding":[11],"in":[12],"plants.":[13],"Solanum":[14,24],"villosum":[15,62,185],"Mill.":[16],"tetraploid":[19],"species":[20],"of":[21,42,60,126,142,164,199],"the":[22,40,73,82,176],"genus":[23],"that":[25,92,175],"possesses":[26],"both":[27],"nutritional":[28],"medicinal":[30],"properties.":[31],"However,":[32],"its":[33],"genetic":[34],"improvement":[35,198],"has":[36],"been":[37],"constrained":[38],"by":[39,158],"lack":[41],"efficient":[43],"systems.":[45],"In":[46],"this":[47,200],"study,":[48],"robust":[50],"protocol":[51],"was":[52,137,156],"established":[53,182],"shoot":[55],"regeneration":[56],"Agrobacterium-mediated":[58],"S.":[61,184],"using":[63],"leaf":[64,94],"explants":[65,95],"A.":[67],"tumefaciens":[68],"strain":[69],"EHA105/pBI35S::GFP,":[70],"which":[71],"harboring":[72],"Neomycin":[74],"phosphotransferase":[75],"II":[76],"(NPT":[77],"II)":[78],"selectable":[79],"marker":[80],"green":[83],"fluorescence":[84,162],"protein":[85],"(GFP)":[86],"reporter":[87],"gene.":[88],"The":[89],"results":[90,173],"showed":[91,145],"100%":[93],"produced":[96],"adventitious":[97],"shoots":[98,128],"on":[99],"semi-solid":[100],"full-strength":[101],"MS":[102],"medium":[103],"containing":[104],"1.0":[105],"mg":[106,111,120],"L\u22121":[107,112,121],"thidiazuron":[108],"(TDZ,)":[109],"0.1":[110],"\u03b1-naphthaleneacetic":[113],"acid":[114],"(NAA),":[115],"0.7%":[116],"agar":[117],"10":[119],"AgNO3,":[122],"with":[123],"an":[124],"average":[125],"7.8":[127],"per":[129],"explant":[130],"after":[131],"four":[132],"weeks.":[133],"Furthermore,":[134],"frequency":[136],"81%,":[138],"over":[140],"90%":[141],"kanamycin-resistant":[143],"plantlets":[144],"normal":[147],"phenotype":[148],"stable":[150],"GFP":[151],"fluorescence.":[152],"Moreover,":[153],"transgene":[154],"integration":[155],"verified":[157],"analyses":[160],"imaging":[163],"flowers,":[165],"fruits,":[166],"T1":[168],"seedlings.":[169],"Taken":[170],"together,":[171],"these":[172],"demonstrate":[174],"tissue":[177],"culture":[178],"system":[181],"provides":[186],"valuable":[188],"platform":[189],"large-scale":[191],"propagation,":[192],"gene":[193],"function":[194],"studies,":[195],"biotechnological":[197],"species.":[201]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2026-10-13T00:00:00"}
