{"id":"https://openalex.org/W7221521972","doi":"https://doi.org/10.64898/2026.10.09.757842","title":"A chemigenetic fluorescence lifetime-based biosensor for imaging of ERK activity","display_name":"A chemigenetic fluorescence lifetime-based biosensor for imaging of ERK activity","publication_year":2026,"publication_date":"2026-10-12","ids":{"openalex":"https://openalex.org/W7221521972","doi":"https://doi.org/10.64898/2026.10.09.757842"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.09.757842","doi":"https://doi.org/10.64898/2026.10.09.757842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.09.757842","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/12/2026.10.09.757842.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/12/2026.10.09.757842.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5120522191","display_name":"Elias Dressler","orcid":"https://orcid.org/0009-0006-7759-6852","observed_orcids":["https://orcid.org/0009-0006-7759-6852"]},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Elias Dressler","raw_affiliation_strings":["Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0009-0006-7759-6852","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035320168","display_name":"Rohan Eapen","orcid":"https://orcid.org/0000-0001-7269-3633","observed_orcids":["https://orcid.org/0000-0001-7269-3633"]},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Rohan S. Eapen","raw_affiliation_strings":["Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0000-0001-7269-3633","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5138794451","display_name":"Dennis Pellanda","orcid":"https://orcid.org/0009-0009-6131-3397","observed_orcids":["https://orcid.org/0009-0009-6131-3397"]},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Dennis Pellanda","raw_affiliation_strings":["Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0009-0009-6131-3397","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158446893","display_name":"Yixin Zhang","orcid":"https://orcid.org/0009-0008-8348-4587","observed_orcids":["https://orcid.org/0009-0008-8348-4587"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Yixin Zhang","raw_affiliation_strings":["Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0009-0008-8348-4587","affiliations":[{"raw_affiliation_string":"Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076377889","display_name":"J\u00e9r\u00f4me Julmi","orcid":"https://orcid.org/0009-0000-0497-7255","observed_orcids":["https://orcid.org/0009-0000-0497-7255"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"J\u00e9r\u00f4me Julmi","raw_affiliation_strings":["Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0009-0000-0497-7255","affiliations":[{"raw_affiliation_string":"Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030116386","display_name":"Musadiq Ahmad Bhat","orcid":"https://orcid.org/0000-0002-0894-9996","observed_orcids":["https://orcid.org/0000-0002-0894-9996"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Musadiq A. Bhat","raw_affiliation_strings":["Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0000-0002-0894-9996","affiliations":[{"raw_affiliation_string":"Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065616575","display_name":"Tommaso Patriarchi","orcid":"https://orcid.org/0000-0001-9351-3734","observed_orcids":["https://orcid.org/0000-0001-9351-3734"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]},{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Tommaso Patriarchi","raw_affiliation_strings":["Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland","Neuroscience Center Zurich, University and ETH Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0000-0001-9351-3734","affiliations":[{"raw_affiliation_string":"Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I202697423"]},{"raw_affiliation_string":"Neuroscience Center Zurich, University and ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I202697423","https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000867962","display_name":"Darren T. Gilmour","orcid":"https://orcid.org/0000-0001-7613-090X","observed_orcids":["https://orcid.org/0000-0001-7613-090X"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Darren Gilmour","raw_affiliation_strings":["Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0000-0001-7613-090X","affiliations":[{"raw_affiliation_string":"Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050812532","display_name":"Michelle S. Frei","orcid":"https://orcid.org/0000-0002-4799-4554","observed_orcids":["https://orcid.org/0000-0002-4799-4554"]},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":true,"raw_author_name":"Michelle S. Frei","raw_affiliation_strings":["Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0000-0002-4799-4554","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5050812532"],"corresponding_institution_ids":["https://openalex.org/I35440088"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10532","display_name":"Molecular Sensors and Ion Detection","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/erk-activity","display_name":"ERK activity","score":0.8029999732971191},{"id":"https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy","display_name":"fluorescence lifetime imaging","score":0.7789999842643738},{"id":"https://openalex.org/keywords/halotag","display_name":"HaloTag","score":0.8389999866485596},{"id":"https://openalex.org/keywords/calcium-signaling","display_name":"calcium signaling","score":0.5189999938011169},{"id":"https://openalex.org/keywords/camp","display_name":"cyclic AMP","score":0.6990000009536743},{"id":"https://openalex.org/keywords/cortical-neurons","display_name":"cortical neurons","score":0.8180000185966492},{"id":"https://openalex.org/keywords/deep-tissue-imaging","display_name":"deep tissue imaging","score":0.7870000004768372},{"id":"https://openalex.org/keywords/live-animal-imaging","display_name":"live animal imaging","score":0.6330000162124634},{"id":"https://openalex.org/keywords/3d-imaging","display_name":"3D imaging","score":0.7279999852180481}],"concepts":[{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.8234000205993652},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.7057999968528748},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6122000217437744},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.6057000160217285},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5669999718666077},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5419999957084656},{"id":"https://openalex.org/C163235415","wikidata":"https://www.wikidata.org/wiki/Q899814","display_name":"Second messenger system","level":3,"score":0.49559998512268066},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.4860999882221222},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4648999869823456},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.453000009059906},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.42080000042915344},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.400299996137619},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.3750999867916107},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.36739999055862427},{"id":"https://openalex.org/C2776991684","wikidata":"https://www.wikidata.org/wiki/Q190012","display_name":"Adenosine","level":2,"score":0.3635999858379364},{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.3625999987125397},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.336899995803833},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.31769999861717224},{"id":"https://openalex.org/C125209161","wikidata":"https://www.wikidata.org/wiki/Q422816","display_name":"Enzyme activator","level":3,"score":0.3043000102043152},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.30090001225471497},{"id":"https://openalex.org/C3019336641","wikidata":"https://www.wikidata.org/wiki/Q55643886","display_name":"Fluorescent protein","level":4,"score":0.29789999127388},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.28929999470710754},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.28459998965263367},{"id":"https://openalex.org/C2776032975","wikidata":"https://www.wikidata.org/wiki/Q5018833","display_name":"Calcium imaging","level":3,"score":0.2833000123500824},{"id":"https://openalex.org/C74254510","wikidata":"https://www.wikidata.org/wiki/Q7240508","display_name":"Premovement neuronal activity","level":2,"score":0.2669000029563904},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.262800008058548},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.26030001044273376}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.10.09.757842","doi":"https://doi.org/10.64898/2026.10.09.757842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.09.757842","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/12/2026.10.09.757842.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:www.research-collection.ethz.ch:20.500.11850/808653","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.11850/808653","pdf_url":null,"source":{"id":"https://openalex.org/S4306402302","display_name":"Repository for Publications and Research Data (ETH Zurich)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I35440088","host_organization_name":"ETH Zurich","host_organization_lineage":["https://openalex.org/I35440088"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"info:eu-repo/semantics/workingPaper"},{"id":"doi:10.3929/ethz-c-000808653","doi":"https://doi.org/10.3929/ethz-c-000808653","is_oa":true,"landing_page_url":"https://doi.org/10.3929/ethz-c-000808653","pdf_url":null,"source":{"id":"https://openalex.org/S7407051236","display_name":"ETH Z\u00fcrich Research Collection","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.64898/2026.10.09.757842","doi":"https://doi.org/10.64898/2026.10.09.757842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.09.757842","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/12/2026.10.09.757842.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2138814873","display_name":null,"funder_award_id":"320030-236030","funder_id":"https://openalex.org/F4320320924","funder_display_name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung"},{"id":"https://openalex.org/G8791997479","display_name":null,"funder_award_id":"CR00I5-235927","funder_id":"https://openalex.org/F4320320924","funder_display_name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung"},{"id":"https://openalex.org/G880118091","display_name":null,"funder_award_id":"320030","funder_id":"https://openalex.org/F4320320924","funder_display_name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320320924","display_name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","ror":"https://ror.org/00yjd3n13"},{"id":"https://openalex.org/F4320321652","display_name":"Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich","ror":"https://ror.org/05a28rw58"},{"id":"https://openalex.org/F4320323874","display_name":"Universit\u00e4t Z\u00fcrich","ror":"https://ror.org/02crff812"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7221521972.pdf","grobid_xml":"https://content.openalex.org/works/W7221521972.grobid-xml"},"referenced_works_count":3,"referenced_works":["https://openalex.org/W4409988818","https://openalex.org/W4411463597","https://openalex.org/W7203833057"],"related_works":["https://openalex.org/W2040172334","https://openalex.org/W2560212278","https://openalex.org/W2015084440","https://openalex.org/W2574241107","https://openalex.org/W4241078300","https://openalex.org/W7099144250","https://openalex.org/W4323533295","https://openalex.org/W4416187580","https://openalex.org/W4391922257","https://openalex.org/W2914466450"],"abstract_inverted_index":{"Abstract":[0],"Extracellular":[1],"signal-regulated":[2],"kinase":[3],"(ERK)":[4],"governs":[5],"many":[6],"cellular":[7],"processes,":[8],"and":[9,76,97,124],"its":[10],"spatiotemporal":[11],"activity":[12,23,88,116,135],"dynamics":[13],"are":[14],"important":[15],"for":[16],"downstream":[17],"signaling":[18],"specificity.":[19],"Existing":[20],"fluorescent":[21],"ERK":[22,48,78,87,115,134],"biosensors,":[24],"however,":[25],"fall":[26],"short":[27],"in":[28,52,93,117,136,142],"enabling":[29],"spectrally":[30],"multiplexed,":[31],"spatially":[32],"resolved,":[33],"or":[34],"deep":[35],"tissue":[36],"measurements.":[37],"Here,":[38],"we":[39],"introduce":[40],"a":[41],"chemigenetic,":[42],"single-fluorophore":[43],"biosensor,":[44],"WHaloEKAR,":[45],"that":[46],"reports":[47],"activities":[49],"through":[50],"changes":[51,59],"fluorescence":[53,103],"lifetime.":[54],"WHaloEKAR":[55,129],"combines":[56],"large":[57],"lifetime":[58,104],"(\u22120.81":[60],"ns)":[61],"with":[62],"the":[63,67,83,120,131],"spectral":[64],"versatility":[65],"of":[66,85,133],"self-labeling":[68],"protein":[69],"HaloTag":[70],"giving":[71],"access":[72],"to":[73,113,119],"orange,":[74],"red,":[75],"far-red":[77],"biosensors.":[79],"The":[80],"latter":[81],"enabled":[82,130],"investigation":[84,132],"heterogeneous":[86],"at":[89],"distinct":[90],"subcellular":[91],"locations":[92],"cultured":[94],"cell":[95],"lines":[96],"primary":[98],"cortical":[99],"rat":[100],"neurons":[101],"via":[102],"imaging":[105],"microscopy":[106],"(FLIM).":[107],"Moreover,":[108],"multiplexed":[109],"FLIM":[110],"measurements":[111],"allowed":[112],"study":[114],"parallel":[118],"second":[121],"messengers":[122],"calcium":[123],"cyclic":[125],"adenosine":[126],"monophosphate.":[127],"Finally,":[128],"3D":[137],"samples":[138],"as":[139,141],"well":[140],"live":[143],"animals.":[144]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-13T00:00:00"}
