{"id":"https://openalex.org/W7221452432","doi":"https://doi.org/10.64898/2026.10.04.756053","title":"Genome-engineered C. elegans strains with sex-transformed neurons and gut","display_name":"Genome-engineered C. elegans strains with sex-transformed neurons and gut","publication_year":2026,"publication_date":"2026-10-11","ids":{"openalex":"https://openalex.org/W7221452432","doi":"https://doi.org/10.64898/2026.10.04.756053"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.04.756053","doi":"https://doi.org/10.64898/2026.10.04.756053","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.04.756053","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/11/2026.10.04.756053.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/11/2026.10.04.756053.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000180611","display_name":"Chien\u2010Po Liao","orcid":"https://orcid.org/0000-0002-4167-1826","observed_orcids":["https://orcid.org/0000-0002-4167-1826"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chien-Po Liao","raw_affiliation_strings":["Columbia University, Howard Hughes Medical Institute, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Columbia University, Howard Hughes Medical Institute, New York, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158297868","display_name":"Hongzhu Ji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hongzhu Ji","raw_affiliation_strings":["Columbia University, Howard Hughes Medical Institute, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Columbia University, Howard Hughes Medical Institute, New York, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I78577930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5041472770","display_name":"Oliver Hobert","orcid":"https://orcid.org/0000-0002-7634-2854","observed_orcids":["https://orcid.org/0000-0002-7634-2854","https://orcid.org/0000-0002-3068-068X"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Oliver Hobert","raw_affiliation_strings":["Columbia University, Howard Hughes Medical Institute, New York, USA"],"raw_orcid":"https://orcid.org/0000-0002-7634-2854","affiliations":[{"raw_affiliation_string":"Columbia University, Howard Hughes Medical Institute, New York, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5041472770"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I78577930"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11077","display_name":"Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities","score":0.6689000129699707,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11077","display_name":"Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities","score":0.6689000129699707,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.08290000259876251,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.0746999979019165,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.8389999866485596},{"id":"https://openalex.org/keywords/trans-splicing","display_name":"trans-splicing","score":0.9480000138282776},{"id":"https://openalex.org/keywords/rab3","display_name":"Rab3","score":0.9279999732971191},{"id":"https://openalex.org/keywords/elt-2","display_name":"ELT-2","score":1.0},{"id":"https://openalex.org/keywords/sex-specific-gene-expression","display_name":"sex-specific gene expression","score":0.7490000128746033},{"id":"https://openalex.org/keywords/caenorhabditis-elegans","display_name":"Caenorhabditis elegans","score":0.9559999704360962}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8756999969482422},{"id":"https://openalex.org/C2778944004","wikidata":"https://www.wikidata.org/wiki/Q91703","display_name":"Caenorhabditis elegans","level":3,"score":0.8579999804496765},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6535000205039978},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.6456000208854675},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6090999841690063},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5455999970436096},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.45579999685287476},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.44999998807907104},{"id":"https://openalex.org/C121346293","wikidata":"https://www.wikidata.org/wiki/Q1340715","display_name":"Endogenous retrovirus","level":4,"score":0.4350999891757965},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3400000035762787},{"id":"https://openalex.org/C91779695","wikidata":"https://www.wikidata.org/wiki/Q3780824","display_name":"Coding region","level":3,"score":0.3269999921321869},{"id":"https://openalex.org/C19843653","wikidata":"https://www.wikidata.org/wiki/Q213907","display_name":"Model organism","level":3,"score":0.32269999384880066},{"id":"https://openalex.org/C2776496032","wikidata":"https://www.wikidata.org/wiki/Q1904768","display_name":"Caenorhabditis","level":4,"score":0.32260000705718994},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.3093000054359436},{"id":"https://openalex.org/C2778830712","wikidata":"https://www.wikidata.org/wiki/Q5185","display_name":"Nematode","level":2,"score":0.3084999918937683},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.2915000021457672},{"id":"https://openalex.org/C61727976","wikidata":"https://www.wikidata.org/wiki/Q751748","display_name":"Epistasis","level":3,"score":0.29010000824928284},{"id":"https://openalex.org/C207177822","wikidata":"https://www.wikidata.org/wiki/Q5050812","display_name":"Genetic screen","level":4,"score":0.2856999933719635},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.27309998869895935},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.25870001316070557}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.04.756053","doi":"https://doi.org/10.64898/2026.10.04.756053","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.04.756053","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/11/2026.10.04.756053.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.04.756053","doi":"https://doi.org/10.64898/2026.10.04.756053","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.04.756053","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/11/2026.10.04.756053.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7221452432.pdf","grobid_xml":"https://content.openalex.org/works/W7221452432.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W171906849","https://openalex.org/W1581412826","https://openalex.org/W1940712597","https://openalex.org/W2010521274","https://openalex.org/W2017888677","https://openalex.org/W2022223287","https://openalex.org/W2035694260","https://openalex.org/W2041514166","https://openalex.org/W2054679205","https://openalex.org/W2056661654","https://openalex.org/W2057634665","https://openalex.org/W2062448203","https://openalex.org/W2064746954","https://openalex.org/W2080730628","https://openalex.org/W2092135843","https://openalex.org/W2101059904","https://openalex.org/W2106258135","https://openalex.org/W2106742994","https://openalex.org/W2106949927","https://openalex.org/W2122163057","https://openalex.org/W2140722339","https://openalex.org/W2146226296","https://openalex.org/W2149633773","https://openalex.org/W2155150900","https://openalex.org/W2184223593","https://openalex.org/W2345968315","https://openalex.org/W2556193517","https://openalex.org/W2770213602","https://openalex.org/W2792092989","https://openalex.org/W2950667797","https://openalex.org/W3043858150","https://openalex.org/W4220661375","https://openalex.org/W4378781294","https://openalex.org/W4404307344"],"related_works":["https://openalex.org/W2140656514","https://openalex.org/W4388737182","https://openalex.org/W2043177918","https://openalex.org/W2041554842","https://openalex.org/W2106949927","https://openalex.org/W2011737877","https://openalex.org/W2096182111","https://openalex.org/W2559907033","https://openalex.org/W2133702185","https://openalex.org/W2079377370"],"abstract_inverted_index":{"ABSTRACT":[0],"The":[1],"sexual":[2],"identity":[3],"of":[4,13,68,80,116,140],"specific":[5],"tissues":[6],"can":[7],"be":[8,88],"altered":[9],"through":[10,113,121],"cell-autonomous":[11],"manipulation":[12],"the":[14,19,46,77,105,129,138],"sex":[15,142],"determination":[16],"pathway":[17],"in":[18,52,90],"nematode":[20],"Caenorhabditis":[21],"elegans":[22],".":[23],"Previously":[24],"described":[25],"sexually":[26],"mosaic":[27],"strains":[28,51,126],"carried":[29],"multicopy":[30],"transgenic":[31],"arrays":[32],"that":[33,84],"are":[34,70,85],"potentially":[35],"subject":[36],"to":[37,49,87,136],"transgene":[38],"silencing":[39],"or":[40,62,97],"misexpression":[41],"artefacts.":[42],"We":[43,103],"used":[44],"here":[45],"CRISPR/Cas9":[47],"system":[48,75],"engineer":[50],"which":[53],"coding":[54],"sequences":[55],"for":[56],"either":[57,91],"a":[58,63,73,92],"masculinizing":[59],"agent":[60,65],"(FEM-3)":[61],"feminizing":[64],"(intracellular":[66],"domain":[67],"TRA-2)":[69],"inserted":[71],"with":[72],"trans-splicing":[74],"at":[76],"3\u2019":[78],"end":[79],"endogenous":[81],"genetic":[82],"loci":[83],"known":[86,117],"expressed":[89],"neuron-specific":[93],"(":[94,99],"rab-3":[95],")":[96,101],"gut-specific":[98],"elt-2":[100],"manner.":[102],"validate":[104],"tissue-specific":[106,141],"sex-transformations":[107],"induced":[108],"by":[109],"these":[110],"genomic":[111],"manipulations":[112],"expression":[114],"analysis":[115],"sex-specific":[118],"genes":[119],"and":[120],"assaying":[122],"behavioral":[123],"readouts.":[124],"These":[125],"may":[127],"serve":[128],"worm":[130],"community":[131],"as":[132],"standard":[133],"reference":[134],"tools":[135],"study":[137],"consequences":[139],"transformations.":[143]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-12T00:00:00"}