{"id":"https://openalex.org/W7221008031","doi":"https://doi.org/10.1016/j.xpro.2026.104885","title":"Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells","display_name":"Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells","publication_year":2026,"publication_date":"2026-10-08","ids":{"openalex":"https://openalex.org/W7221008031","doi":"https://doi.org/10.1016/j.xpro.2026.104885","pmid":"https://pubmed.ncbi.nlm.nih.gov/42848514","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13675714"},"language":"en","primary_location":{"id":"doi:10.1016/j.xpro.2026.104885","doi":"https://doi.org/10.1016/j.xpro.2026.104885","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2026.104885","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.xpro.2026.104885","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5094180775","display_name":"Yonca Ke\u015fkek T\u00fcrk","orcid":"https://orcid.org/0000-0002-7955-086X","observed_orcids":["https://orcid.org/0000-0002-7955-086X"]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]},{"id":"https://openalex.org/I52099693","display_name":"University of York","ror":"https://ror.org/04m01e293","country_code":"GB","type":"education","lineage":["https://openalex.org/I52099693"]},{"id":"https://openalex.org/I66514158","display_name":"Hacettepe University","ror":"https://ror.org/04kwvgz42","country_code":"TR","type":"education","lineage":["https://openalex.org/I66514158"]},{"id":"https://openalex.org/I69768126","display_name":"Hull York Medical School","ror":"https://ror.org/0003e4m70","country_code":"GB","type":"education","lineage":["https://openalex.org/I69768126"]}],"countries":["GB","TR"],"is_corresponding":true,"raw_author_name":"Yonca Keskek Turk","raw_affiliation_strings":["School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, T\u00fcrkiye. Electronic address: yoncaturk@hacettepe.edu.tr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, T\u00fcrkiye. Electronic address: yoncaturk@hacettepe.edu.tr","institution_ids":["https://openalex.org/I130828816","https://openalex.org/I52099693","https://openalex.org/I66514158","https://openalex.org/I69768126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5120134701","display_name":"Ailish McCafferty-Brown","orcid":"https://orcid.org/0000-0002-4983-2014","observed_orcids":["https://orcid.org/0000-0002-4983-2014"]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]},{"id":"https://openalex.org/I52099693","display_name":"University of York","ror":"https://ror.org/04m01e293","country_code":"GB","type":"education","lineage":["https://openalex.org/I52099693"]},{"id":"https://openalex.org/I69768126","display_name":"Hull York Medical School","ror":"https://ror.org/0003e4m70","country_code":"GB","type":"education","lineage":["https://openalex.org/I69768126"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Ailish McCafferty-Brown","raw_affiliation_strings":["School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I130828816","https://openalex.org/I52099693","https://openalex.org/I69768126","https://openalex.org/I98677209"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040423570","display_name":"Clive S. McKimmie","orcid":"https://orcid.org/0000-0002-7694-9509","observed_orcids":["https://orcid.org/0000-0002-7694-9509"]},"institutions":[{"id":"https://openalex.org/I130828816","display_name":"University of Leeds","ror":"https://ror.org/024mrxd33","country_code":"GB","type":"education","lineage":["https://openalex.org/I130828816"]},{"id":"https://openalex.org/I52099693","display_name":"University of York","ror":"https://ror.org/04m01e293","country_code":"GB","type":"education","lineage":["https://openalex.org/I52099693"]},{"id":"https://openalex.org/I69768126","display_name":"Hull York Medical School","ror":"https://ror.org/0003e4m70","country_code":"GB","type":"education","lineage":["https://openalex.org/I69768126"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Clive S. McKimmie","raw_affiliation_strings":["School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK. Electronic address: clive.mckimmie@york.ac.uk"],"raw_orcid":"https://orcid.org/0000-0002-7694-9509","affiliations":[{"raw_affiliation_string":"School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK. Electronic address: clive.mckimmie@york.ac.uk","institution_ids":["https://openalex.org/I130828816","https://openalex.org/I52099693","https://openalex.org/I69768126"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5040423570","https://openalex.org/A5094180775"],"corresponding_institution_ids":["https://openalex.org/I130828816","https://openalex.org/I52099693","https://openalex.org/I66514158","https://openalex.org/I69768126"],"apc_list":{"value":1550,"currency":"USD","value_usd":1550},"apc_paid":{"value":1550,"currency":"USD","value_usd":1550},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.76337416,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"7","issue":"4","first_page":"104885","last_page":"104885"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12047","display_name":"Viral Infections and Vectors","score":0.9326000213623047,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12047","display_name":"Viral Infections and Vectors","score":0.9326000213623047,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.04309999942779541,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/toscana-virus","display_name":"Toscana virus","score":1.0},{"id":"https://openalex.org/keywords/arbovirus-infection","display_name":"arbovirus infection","score":0.8240000009536743},{"id":"https://openalex.org/keywords/sand-fly-saliva","display_name":"sand fly saliva","score":0.6460000276565552},{"id":"https://openalex.org/keywords/dermal-fibroblasts","display_name":"dermal fibroblasts","score":0.9129999876022339},{"id":"https://openalex.org/keywords/skin-transcriptomics","display_name":"skin transcriptomics","score":0.7099999785423279},{"id":"https://openalex.org/keywords/single-cell-suspension","display_name":"single-cell suspension","score":0.5410000085830688},{"id":"https://openalex.org/keywords/subcutaneous-inoculation","display_name":"subcutaneous inoculation","score":0.8230000138282776}],"concepts":[{"id":"https://openalex.org/C2776207758","wikidata":"https://www.wikidata.org/wiki/Q5303302","display_name":"Downstream (manufacturing)","level":2,"score":0.5278000235557556},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.48330000042915344},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.46970000863075256},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4447000026702881},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.41769999265670776},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.41600000858306885},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32710000872612},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.31360000371932983},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.3084000051021576},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.3061999976634979},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.2903999984264374},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2671999931335449}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1016/j.xpro.2026.104885","doi":"https://doi.org/10.1016/j.xpro.2026.104885","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2026.104885","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},{"id":"pmid:42848514","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42848514","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR protocols","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13675714","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13675714/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protoc","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:6af3607482444092bfffb78d145fed0c","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/6af3607482444092bfffb78d145fed0c","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"STAR Protocols, Vol 7, Iss 4, Pp 104885- (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.xpro.2026.104885","doi":"https://doi.org/10.1016/j.xpro.2026.104885","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2026.104885","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4821391275","display_name":"DTP Bid Led by University of Sheffield","funder_award_id":"MR/N013840/1","funder_id":"https://openalex.org/F4320334626","funder_display_name":"Medical Research Council"}],"funders":[{"id":"https://openalex.org/F4320310935","display_name":"University of York","ror":"https://ror.org/04m01e293"},{"id":"https://openalex.org/F4320334626","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W2011247707","https://openalex.org/W2120017307","https://openalex.org/W3014073311","https://openalex.org/W3014614048","https://openalex.org/W3082430816","https://openalex.org/W4282921302","https://openalex.org/W4404138384","https://openalex.org/W4415547493"],"related_works":["https://openalex.org/W4415547493","https://openalex.org/W7057209250","https://openalex.org/W4361255804","https://openalex.org/W2468379385","https://openalex.org/W2011247707","https://openalex.org/W2172000541","https://openalex.org/W3017359036","https://openalex.org/W2343147051","https://openalex.org/W7099160807","https://openalex.org/W4382403111"],"abstract_inverted_index":{"Here,":[0],"we":[1,57],"present":[2],"an":[3],"in":[4,16],"vivo":[5],"approach":[6],"to":[7,89,112],"study":[8],"virus-vector-host":[9],"interactions":[10],"during":[11],"Toscana":[12],"virus":[13],"(TOSV)":[14],"infection":[15,115],"mouse":[17],"skin.":[18],"We":[19,46],"describe":[20,58],"preparation":[21],"of":[22,53,62,67,84],"viral":[23],"stocks":[24],"and":[25,51,70,82],"subcutaneous":[26],"TOSV":[27],"inoculation":[28],"into":[29],"dorsal":[30],"upper":[31],"foot":[32],"skin,":[33],"either":[34],"alone,":[35],"with":[36],"sand-fly":[37,44],"salivary":[38],"gland":[39],"extract":[40],"(SGE),":[41],"or":[42],"following":[43],"biting.":[45],"then":[47],"detail":[48],"tissue":[49],"collection":[50],"generation":[52],"single-cell":[54],"suspensions.":[55],"Finally,":[56],"flow":[59],"cytometric":[60],"detection":[61],"infected":[63],"cells,":[64],"magnetic":[65],"isolation":[66],"dermal":[68],"fibroblasts,":[69],"RNA":[71],"extraction":[72],"for":[73],"transcriptomic":[74],"analysis.":[75],"For":[76],"complete":[77],"details":[78],"on":[79],"the":[80],"use":[81],"execution":[83],"this":[85],"protocol,":[86],"please":[87],"refer":[88],"Keskek":[90,96],"Turk":[91],"et":[92],"al.":[93],"1":[94],"1.":[95],"Turk,":[97],"Y.":[98],"\u2219":[99,102],"McCafferty-Brown,":[100],"A.":[101],"Barningham,":[103],"L.":[104],"...":[105],"Sand":[106],"fly":[107],"saliva":[108],"reprograms":[109],"skin":[110],"fibroblasts":[111],"enhance":[113],"arbovirus":[114],"iScience.":[116],"2025;":[117],"28":[118],",":[119],"113854":[120],"Full":[121,123],"Text":[122,124],"(PDF)":[125],"Scopus":[126],"(1)":[127],"PubMed":[128],"Google":[129],"Scholar":[130]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-09T00:00:00"}