{"id":"https://openalex.org/W7220956424","doi":"https://doi.org/10.21203/rs.3.rs-11320065/v1","title":"Gfa, a new integron-borne fosfomycin resistance protein family","display_name":"Gfa, a new integron-borne fosfomycin resistance protein family","publication_year":2026,"publication_date":"2026-10-08","ids":{"openalex":"https://openalex.org/W7220956424","doi":"https://doi.org/10.21203/rs.3.rs-11320065/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11320065/v1","doi":"https://doi.org/10.21203/rs.3.rs-11320065/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11320065/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.21203/rs.3.rs-11320065/v1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050627259","display_name":"Nicolas Kieffer","orcid":"https://orcid.org/0000-0002-2672-1127","observed_orcids":["https://orcid.org/0000-0002-2672-1127"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Nicolas Kieffer","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104303556","display_name":"Amalia Prieto","orcid":"https://orcid.org/0009-0000-1280-5875","observed_orcids":["https://orcid.org/0009-0000-1280-5875"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Amalia Prieto","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104343842","display_name":"Laura Ortiz-Miravalles","orcid":"https://orcid.org/0009-0009-1354-0683","observed_orcids":["https://orcid.org/0009-0009-1354-0683"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Laura Ortiz-Miravalles","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084599189","display_name":"Alberto Hip\u00f3lito","orcid":"https://orcid.org/0000-0002-6461-2650","observed_orcids":["https://orcid.org/0000-0002-6461-2650"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Alberto Hip\u00f3lito","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002601718","display_name":"Ester Vergara","orcid":"https://orcid.org/0000-0003-1323-4749","observed_orcids":["https://orcid.org/0000-0003-1323-4749"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Ester Vergara","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089393924","display_name":"Juan Borrero","orcid":"https://orcid.org/0000-0002-9203-0551","observed_orcids":["https://orcid.org/0000-0002-9203-0551"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Juan Borrero","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157894997","display_name":"Andre Carvalho","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Andr\u00e9 Carvalho","raw_affiliation_strings":["Universidad Complutense de Madrid"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidad Complutense de Madrid","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062131591","display_name":"Jos\u00e9 Antonio Escudero","orcid":"https://orcid.org/0000-0001-8552-2956","observed_orcids":["https://orcid.org/0000-0001-8552-2956"]},"institutions":[{"id":"https://openalex.org/I4210104776","display_name":"Centro Nacional de Biotecnolog\u00eda","ror":"https://ror.org/015w4v032","country_code":"ES","type":"facility","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210104776"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Jos\u00e9 Antonio Escudero","raw_affiliation_strings":["Centro Nacional de Biotecnolog\u00eda"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro Nacional de Biotecnolog\u00eda","institution_ids":["https://openalex.org/I4210104776"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10419","display_name":"Pharmaceutical and Antibiotic Environmental Impacts","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gfa","display_name":"GFA","score":0.8420000076293945},{"id":"https://openalex.org/keywords/fosfomycin-resistance","display_name":"fosfomycin resistance","score":0.9959999918937683},{"id":"https://openalex.org/keywords/integron-gene-cassettes","display_name":"integron gene cassettes","score":0.9210000038146973},{"id":"https://openalex.org/keywords/multidrug-resistance","display_name":"multidrug resistance","score":0.7089999914169312},{"id":"https://openalex.org/keywords/enterobacterales","display_name":"Enterobacterales","score":0.871999979019165},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":1.0},{"id":"https://openalex.org/keywords/zinc-binding-proteins","display_name":"zinc-binding proteins","score":0.4230000078678131},{"id":"https://openalex.org/keywords/cysteine-rich-domain","display_name":"cysteine-rich domain","score":0.7889999747276306}],"concepts":[{"id":"https://openalex.org/C2778897966","wikidata":"https://www.wikidata.org/wiki/Q183554","display_name":"Fosfomycin","level":3,"score":0.991599977016449},{"id":"https://openalex.org/C2777637488","wikidata":"https://www.wikidata.org/wiki/Q31856","display_name":"Pseudomonas aeruginosa","level":3,"score":0.6139000058174133},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5788999795913696},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5637000203132629},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5526999831199646},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5098999738693237},{"id":"https://openalex.org/C2776650774","wikidata":"https://www.wikidata.org/wiki/Q420197","display_name":"Integron","level":4,"score":0.505299985408783},{"id":"https://openalex.org/C94665300","wikidata":"https://www.wikidata.org/wiki/Q63391344","display_name":"Antibiotic resistance","level":3,"score":0.45159998536109924},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4077000021934509},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.3971000015735626},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36550000309944153},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3440000116825104},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.3208000063896179},{"id":"https://openalex.org/C114851261","wikidata":"https://www.wikidata.org/wiki/Q12147416","display_name":"Drug resistance","level":2,"score":0.27399998903274536},{"id":"https://openalex.org/C57473165","wikidata":"https://www.wikidata.org/wiki/Q7315604","display_name":"Resistance (ecology)","level":2,"score":0.27239999175071716},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.26100000739097595},{"id":"https://openalex.org/C2778910516","wikidata":"https://www.wikidata.org/wiki/Q380136","display_name":"Enterobacteriaceae","level":4,"score":0.25519999861717224}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.21203/rs.3.rs-11320065/v1","doi":"https://doi.org/10.21203/rs.3.rs-11320065/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11320065/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},{"id":"doi:10.64898/2026.10.07.757220","doi":"https://doi.org/10.64898/2026.10.07.757220","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.07.757220","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.07.757220.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11320065/v1","doi":"https://doi.org/10.21203/rs.3.rs-11320065/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11320065/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1016824299","display_name":"UNA Europa, an alliance of universities FOR the emergence of talent and the development of research CAREERs","funder_award_id":"847635","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G3653216052","display_name":null,"funder_award_id":"PCI2021-122024-2A","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G5353759257","display_name":null,"funder_award_id":"CNS2022-135857","funder_id":"https://openalex.org/F4320322930","funder_display_name":"Ministerio de Ciencia e Innovaci\u00f3n"},{"id":"https://openalex.org/G717256432","display_name":null,"funder_award_id":"PID2024","funder_id":"https://openalex.org/F4320315062","funder_display_name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades"},{"id":"https://openalex.org/G8681408770","display_name":null,"funder_award_id":"FPU21","funder_id":"https://openalex.org/F4320322930","funder_display_name":"Ministerio de Ciencia e Innovaci\u00f3n"}],"funders":[{"id":"https://openalex.org/F4320309764","display_name":"Fundaci\u00f3n BBVA","ror":"https://ror.org/01jzs3b90"},{"id":"https://openalex.org/F4320314161","display_name":"Joint Programming Initiative on Antimicrobial Resistance","ror":null},{"id":"https://openalex.org/F4320315062","display_name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","ror":null},{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320322930","display_name":"Ministerio de Ciencia e Innovaci\u00f3n","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220956424.pdf","grobid_xml":"https://content.openalex.org/works/W7220956424.grobid-xml"},"referenced_works_count":5,"referenced_works":["https://openalex.org/W1977601185","https://openalex.org/W1977687525","https://openalex.org/W2101587480","https://openalex.org/W2978604744","https://openalex.org/W7134285610"],"related_works":["https://openalex.org/W4405740974","https://openalex.org/W2156180900","https://openalex.org/W7154475300","https://openalex.org/W2750743848","https://openalex.org/W3167152592","https://openalex.org/W7124172700","https://openalex.org/W3104343646","https://openalex.org/W3088854452","https://openalex.org/W2414397525","https://openalex.org/W3088384896"],"abstract_inverted_index":{"Abstract":[0],"Fosfomycin":[1],"is":[2,53],"increasingly":[3],"used":[4],"to":[5,55,96],"treat":[6],"multidrug-resistant":[7],"Gram-negative":[8],"infections,":[9],"yet":[10],"resistance":[11,31,46,112,124],"surveillance":[12],"largely":[13],"relies":[14],"on":[15],"homology-based":[16,119],"detection":[17],"of":[18,37,99,118],"known":[19],"fos":[20],"genes.":[21],"Here,":[22],"we":[23],"identify":[24],"Gfa,":[25],"a":[26,63,97,108],"previously":[27,109],"unrecognized":[28,110],"integron-borne":[29],"fosfomycin":[30,45,81,111],"determinant":[32],"uncovered":[33],"through":[34],"functional":[35],"screening":[36],"129":[38],"integron":[39],"gene":[40],"cassettes.":[41],"Gfa":[42,87,106],"confers":[43],"high-level":[44],"across":[47],"Enterobacterales":[48],"and":[49,52,60,71,114,126],"Pseudomonas":[50],"aeruginosa":[51],"resistant":[54],"phosphonoformate":[56],"inhibition.":[57],"Structural":[58],"modelling":[59],"mutagenesis":[61],"reveal":[62],"conserved":[64],"cysteine-rich,":[65],"zinc-organized":[66],"architecture":[67],"essential":[68],"for":[69,121],"activity":[70,93],"distinct":[72],"from":[73],"canonical":[74],"Fos":[75],"enzymes.":[76],"Crude-extract":[77],"assays":[78],"confirm":[79],"glutathione-dependent":[80],"inactivation":[82],"under":[83],"metal-chelating":[84],"conditions.":[85],"Distant":[86],"homologs":[88],"also":[89],"conferred":[90],"resistance,":[91],"although":[92],"was":[94],"restricted":[95],"subset":[98],"proteins":[100],"tested.":[101],"Together,":[102],"these":[103],"findings":[104],"establish":[105],"as":[107],"family":[113,129],"highlight":[115],"the":[116],"limitations":[117],"mining":[120],"discovering":[122],"novel":[123],"determinants":[125],"predicting":[127],"which":[128],"members":[130],"are":[131],"functional.":[132]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2026-10-09T00:00:00"}
