{"id":"https://openalex.org/W7220909511","doi":"https://doi.org/10.1016/j.jbc.2026.113626","title":"Structural and biochemical basis for the formation of \u03b1-(1 \u2192 3)-linked mannose residue in O-glycans of Saccharomyces cerevisiae","display_name":"Structural and biochemical basis for the formation of \u03b1-(1 \u2192 3)-linked mannose residue in O-glycans of Saccharomyces cerevisiae","publication_year":2026,"publication_date":"2026-10-08","ids":{"openalex":"https://openalex.org/W7220909511","doi":"https://doi.org/10.1016/j.jbc.2026.113626","pmid":"https://pubmed.ncbi.nlm.nih.gov/42849732"},"language":"en","primary_location":{"id":"doi:10.1016/j.jbc.2026.113626","doi":"https://doi.org/10.1016/j.jbc.2026.113626","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2026.113626","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.jbc.2026.113626","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083648025","display_name":"Daisuke Hira","orcid":"https://orcid.org/0000-0002-1126-3725","observed_orcids":["https://orcid.org/0000-0002-1126-3725"]},"institutions":[{"id":"https://openalex.org/I181097317","display_name":"Sojo University","ror":"https://ror.org/014fz7968","country_code":"JP","type":"education","lineage":["https://openalex.org/I181097317"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Daisuke Hira","raw_affiliation_strings":["Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University, Kumamoto, Japan. Electronic address: hira@bio.sojo-u.ac.jp"],"raw_orcid":"https://orcid.org/0000-0002-1126-3725","affiliations":[{"raw_affiliation_string":"Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University, Kumamoto, Japan. Electronic address: hira@bio.sojo-u.ac.jp","institution_ids":["https://openalex.org/I181097317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030328414","display_name":"Chihiro Kadooka","orcid":"https://orcid.org/0000-0001-5721-0445","observed_orcids":["https://orcid.org/0000-0001-5721-0445"]},"institutions":[{"id":"https://openalex.org/I181097317","display_name":"Sojo University","ror":"https://ror.org/014fz7968","country_code":"JP","type":"education","lineage":["https://openalex.org/I181097317"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Chihiro Kadooka","raw_affiliation_strings":["Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University, Kumamoto, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University, Kumamoto, Japan","institution_ids":["https://openalex.org/I181097317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054763025","display_name":"Takuji Oka","orcid":"https://orcid.org/0000-0002-5691-654X","observed_orcids":["https://orcid.org/0000-0002-5691-654X"]},"institutions":[{"id":"https://openalex.org/I181097317","display_name":"Sojo University","ror":"https://ror.org/014fz7968","country_code":"JP","type":"education","lineage":["https://openalex.org/I181097317"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Takuji Oka","raw_affiliation_strings":["Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University, Kumamoto, Japan. Electronic address: oka@bio.sojo-u.ac.jp"],"raw_orcid":"https://orcid.org/0000-0002-5691-654X","affiliations":[{"raw_affiliation_string":"Department of Biotechnology and Life Sciences, Faculty of Biotechnology and Life Sciences, Sojo University, Kumamoto, Japan. Electronic address: oka@bio.sojo-u.ac.jp","institution_ids":["https://openalex.org/I181097317"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5054763025","https://openalex.org/A5083648025"],"corresponding_institution_ids":["https://openalex.org/I181097317"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.6279415,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"302","issue":"11","first_page":"113626","last_page":"113626"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mannosyltransferase","display_name":"mannosyltransferase","score":0.8069999814033508},{"id":"https://openalex.org/keywords/o-glycan-biosynthesis","display_name":"O-glycan biosynthesis","score":0.8510000109672546},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9459999799728394},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.8190000057220459},{"id":"https://openalex.org/keywords/dxd-motif","display_name":"DXD motif","score":0.8100000023841858},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.7229999899864197},{"id":"https://openalex.org/keywords/active-site","display_name":"active site","score":0.3540000021457672}],"concepts":[{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.8216000199317932},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7264999747276306},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.6826000213623047},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6650000214576721},{"id":"https://openalex.org/C2775887612","wikidata":"https://www.wikidata.org/wiki/Q10809118","display_name":"Mannose","level":2,"score":0.6556000113487244},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.47200000286102295},{"id":"https://openalex.org/C2778447961","wikidata":"https://www.wikidata.org/wiki/Q1146293","display_name":"Saccharomyces","level":4,"score":0.32829999923706055},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.29120001196861267},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.25060001015663147},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.21660000085830688}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jbc.2026.113626","doi":"https://doi.org/10.1016/j.jbc.2026.113626","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2026.113626","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:42849732","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42849732","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.jbc.2026.113626","doi":"https://doi.org/10.1016/j.jbc.2026.113626","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2026.113626","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":50,"referenced_works":["https://openalex.org/W1031578623","https://openalex.org/W1559639924","https://openalex.org/W1962918695","https://openalex.org/W1965466877","https://openalex.org/W1975456619","https://openalex.org/W1982482490","https://openalex.org/W1983676802","https://openalex.org/W1986191025","https://openalex.org/W2000702379","https://openalex.org/W2001641653","https://openalex.org/W2008708467","https://openalex.org/W2013815918","https://openalex.org/W2028327248","https://openalex.org/W2029667189","https://openalex.org/W2038564024","https://openalex.org/W2045683699","https://openalex.org/W2058571066","https://openalex.org/W2082591831","https://openalex.org/W2085387269","https://openalex.org/W2086291066","https://openalex.org/W2088362281","https://openalex.org/W2095638950","https://openalex.org/W2102611713","https://openalex.org/W2105668062","https://openalex.org/W2112921913","https://openalex.org/W2144081223","https://openalex.org/W2146080841","https://openalex.org/W2147300206","https://openalex.org/W2154714625","https://openalex.org/W2156041732","https://openalex.org/W2156229939","https://openalex.org/W2159211495","https://openalex.org/W2163567142","https://openalex.org/W2306135225","https://openalex.org/W2348723954","https://openalex.org/W2394883609","https://openalex.org/W2404280981","https://openalex.org/W2576983849","https://openalex.org/W2598091503","https://openalex.org/W2614664430","https://openalex.org/W2739115781","https://openalex.org/W2901017189","https://openalex.org/W2913316729","https://openalex.org/W2954851300","https://openalex.org/W2968256998","https://openalex.org/W3010580300","https://openalex.org/W3082370979","https://openalex.org/W3177828909","https://openalex.org/W4248872320","https://openalex.org/W4283726552"],"related_works":["https://openalex.org/W7098497485","https://openalex.org/W1962918695","https://openalex.org/W2102611713","https://openalex.org/W2086291066","https://openalex.org/W2013815918","https://openalex.org/W1994685223","https://openalex.org/W1992062060","https://openalex.org/W2058571066","https://openalex.org/W2085019624","https://openalex.org/W2037205193"],"abstract_inverted_index":{"The":[0,75,106],"biosynthesis":[1],"of":[2,16,32,40,47,58,70,179,191],"O":[3,48,199],"-mannose-type":[4],"glycans":[5],"in":[6,134,197],"the":[7,13,20,38,41,55,65,103,112,126,159,163,168,176,184,189],"budding":[8],"yeast":[9,198],"Saccharomyces":[10],"cerevisiae":[11],"requires":[12],"sequential":[14],"action":[15],"multiple":[17],"mannosyltransferases.":[18],"However,":[19],"molecular":[21,154],"mechanism":[22],"governing":[23],"\u03b1-(1\u21923)-linkage":[24,172],"formation":[25],"remains":[26],"poorly":[27],"understood.":[28],"Mnt2p,":[29],"a":[30,71,82,87,92,97,122],"member":[31],"glycosyltransferase":[33],"family":[34,73],"71":[35],"(GT71),":[36],"catalyzes":[37],"transfer":[39],"fourth":[42],"and":[43,96,118,144,153,193],"fifth":[44],"mannose":[45],"residues":[46],"-glycans":[49],"via":[50],"\u03b1-(1\u21923)-linkages.":[51],"Here,":[52],"we":[53,138],"report":[54],"crystal":[56],"structure":[57,69,76],"Mnt2p":[59,132],"at":[60,111],"2.40":[61],"\u00c5":[62],"resolution,":[63],"representing":[64],"first":[66],"experimentally":[67],"determined":[68,145],"GT71":[72,104],"enzyme.":[74],"revealed":[77,157],"an":[78],"N-terminal":[79],"domain":[80,100,161],"adopting":[81],"canonical":[83],"GT-A":[84],"fold":[85],"harboring":[86],"DXD":[88],"motif":[89],"that":[90,158],"coordinates":[91],"Mn":[93],"2+":[94],"ion":[95],"novel":[98],"C-terminal":[99,160],"unique":[101],"to":[102,121,125],"family.":[105],"active":[107],"site":[108],"is":[109],"formed":[110],"interface":[113],"between":[114],"these":[115],"two":[116],"domains,":[117],"GDP-mannose":[119],"binds":[120],"cleft":[123],"adjacent":[124],"catalytic":[127],"metal":[128],"ion.":[129],"Using":[130],"recombinant":[131],"expressed":[133],"Escherichia":[135],"coli":[136],",":[137],"biochemically":[139],"demonstrated":[140],"its":[141,146],"\u03b1-(1\u21923)-mannosyltransferase":[142],"activity":[143],"substrate":[147],"specificity":[148,178],"toward":[149],"\u03b1-mannobiose":[150],"acceptors.":[151],"Docking":[152],"dynamics":[155],"simulations":[156],"forms":[162],"acceptor":[164],"substrate-binding":[165],"site,":[166],"providing":[167],"structural":[169,185],"basis":[170],"for":[171,187],"selectivity,":[173],"distinct":[174],"from":[175],"linkage":[177],"\u03b1-(1\u21922)-mannosyltransferases.":[180],"These":[181],"findings":[182],"establish":[183],"foundation":[186],"understanding":[188],"differentiation":[190],"\u03b1-(1\u21922)":[192],"\u03b1-(1\u21923)":[194],"mannosyl":[195],"linkages":[196],"-glycan":[200],"biosynthesis.":[201]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-09T00:00:00"}