{"id":"https://openalex.org/W7220865995","doi":"https://doi.org/10.64898/2026.10.01.755830","title":"Sarcoplasmic reticulum remodeling through autophagosome biogenesis during Drosophila metamorphosis","display_name":"Sarcoplasmic reticulum remodeling through autophagosome biogenesis during Drosophila metamorphosis","publication_year":2026,"publication_date":"2026-10-07","ids":{"openalex":"https://openalex.org/W7220865995","doi":"https://doi.org/10.64898/2026.10.01.755830"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.01.755830","doi":"https://doi.org/10.64898/2026.10.01.755830","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.01.755830","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.01.755830.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.01.755830.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5157885567","display_name":"Yuri Kasai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4400009020","display_name":"Institute of Science Tokyo","ror":"https://ror.org/05dqf9946","country_code":"JP","type":"education","lineage":["https://openalex.org/I4400009020"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuri Kasai","raw_affiliation_strings":["School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan","institution_ids":["https://openalex.org/I4400009020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5133976658","display_name":"Shreya Borthakur","orcid":"https://orcid.org/0009-0009-6537-0786","observed_orcids":["https://orcid.org/0009-0009-6537-0786"]},"institutions":[{"id":"https://openalex.org/I4400009020","display_name":"Institute of Science Tokyo","ror":"https://ror.org/05dqf9946","country_code":"JP","type":"education","lineage":["https://openalex.org/I4400009020"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shreya Borthakur","raw_affiliation_strings":["School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan"],"raw_orcid":"https://orcid.org/0009-0009-6537-0786","affiliations":[{"raw_affiliation_string":"School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan","institution_ids":["https://openalex.org/I4400009020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157887193","display_name":"Sabrina Bennani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4400009020","display_name":"Institute of Science Tokyo","ror":"https://ror.org/05dqf9946","country_code":"JP","type":"education","lineage":["https://openalex.org/I4400009020"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Sabrina Bennani","raw_affiliation_strings":["School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan","institution_ids":["https://openalex.org/I4400009020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090861553","display_name":"Yuriko Sakamaki","orcid":"https://orcid.org/0000-0003-0469-4313","observed_orcids":["https://orcid.org/0000-0003-0469-4313"]},"institutions":[{"id":"https://openalex.org/I4400009020","display_name":"Institute of Science Tokyo","ror":"https://ror.org/05dqf9946","country_code":"JP","type":"education","lineage":["https://openalex.org/I4400009020"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuriko Sakamaki","raw_affiliation_strings":["Biomedical Center, Institute of Science Tokyo, Tokyo, 113-8510, Japan"],"raw_orcid":"https://orcid.org/0000-0003-0469-4313","affiliations":[{"raw_affiliation_string":"Biomedical Center, Institute of Science Tokyo, Tokyo, 113-8510, Japan","institution_ids":["https://openalex.org/I4400009020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077680056","display_name":"Tadayoshi Murakawa","orcid":"https://orcid.org/0009-0003-1376-8772","observed_orcids":["https://orcid.org/0009-0003-1376-8772"]},"institutions":[{"id":"https://openalex.org/I4400009020","display_name":"Institute of Science Tokyo","ror":"https://ror.org/05dqf9946","country_code":"JP","type":"education","lineage":["https://openalex.org/I4400009020"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tadayoshi Murakawa","raw_affiliation_strings":["School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan"],"raw_orcid":"https://orcid.org/0009-0003-1376-8772","affiliations":[{"raw_affiliation_string":"School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan","institution_ids":["https://openalex.org/I4400009020"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5090590574","display_name":"Naonobu Fujita","orcid":"https://orcid.org/0000-0003-1914-8438","observed_orcids":["https://orcid.org/0000-0003-1914-8438"]},"institutions":[{"id":"https://openalex.org/I4400009020","display_name":"Institute of Science Tokyo","ror":"https://ror.org/05dqf9946","country_code":"JP","type":"education","lineage":["https://openalex.org/I4400009020"]},{"id":"https://openalex.org/I4405286842","display_name":"Institute of Integrated Research, Institute of Science Tokyo","ror":"https://ror.org/038jk9m46","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4400009020","https://openalex.org/I4405286842"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Naonobu Fujita","raw_affiliation_strings":["Cell Biology Center, Institute of Integrated Research, Institute of Science Tokyo, 4259 S2-11 Nagatsuta, Midori, Yokohama, 226-8501, Japan","School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan"],"raw_orcid":"https://orcid.org/0000-0003-1914-8438","affiliations":[{"raw_affiliation_string":"Cell Biology Center, Institute of Integrated Research, Institute of Science Tokyo, 4259 S2-11 Nagatsuta, Midori, Yokohama, 226-8501, Japan","institution_ids":["https://openalex.org/I4405286842"]},{"raw_affiliation_string":"School of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan","institution_ids":["https://openalex.org/I4400009020"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5090590574"],"corresponding_institution_ids":["https://openalex.org/I4400009020","https://openalex.org/I4405286842"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.00860000029206276,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/autophagosome-biogenesis","display_name":"autophagosome formation","score":0.972000002861023},{"id":"https://openalex.org/keywords/autophagy","display_name":"autophagy","score":0.8629999756813049},{"id":"https://openalex.org/keywords/drosophila-metamorphosis","display_name":"Drosophila metamorphosis","score":0.8759999871253967},{"id":"https://openalex.org/keywords/muscle-remodeling","display_name":"muscle remodeling","score":0.8460000157356262},{"id":"https://openalex.org/keywords/muscle-atrophy","display_name":"muscle atrophy","score":0.75},{"id":"https://openalex.org/keywords/muscle-hypertrophy","display_name":"muscle hypertrophy","score":0.7549999952316284},{"id":"https://openalex.org/keywords/autophagosome-lysosome-fusion","display_name":"autophagosome-lysosome fusion","score":0.8700000047683716},{"id":"https://openalex.org/keywords/intracellular-calcium-stores","display_name":"intracellular calcium stores","score":0.3840000033378601}],"concepts":[{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.8539999723434448},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.8087000250816345},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.776199996471405},{"id":"https://openalex.org/C168296220","wikidata":"https://www.wikidata.org/wiki/Q14882441","display_name":"Autophagosome","level":4,"score":0.6391000151634216},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5288000106811523},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.4246000051498413},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.4230000078678131},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40059998631477356},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.36640000343322754},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.35339999198913574},{"id":"https://openalex.org/C2780104201","wikidata":"https://www.wikidata.org/wiki/Q130888","display_name":"Drosophila melanogaster","level":3,"score":0.3513999879360199},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.3431999981403351},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.33709999918937683},{"id":"https://openalex.org/C187092789","wikidata":"https://www.wikidata.org/wiki/Q4654662","display_name":"ATG16L1","level":4,"score":0.31619998812675476},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.31299999356269836},{"id":"https://openalex.org/C63645605","wikidata":"https://www.wikidata.org/wiki/Q103191","display_name":"Homeostasis","level":2,"score":0.3075999915599823},{"id":"https://openalex.org/C2776263037","wikidata":"https://www.wikidata.org/wiki/Q1557686","display_name":"Muscle atrophy","level":3,"score":0.3019999861717224},{"id":"https://openalex.org/C139966638","wikidata":"https://www.wikidata.org/wiki/Q597678","display_name":"Sarcoplasm","level":3,"score":0.2928999960422516},{"id":"https://openalex.org/C98539663","wikidata":"https://www.wikidata.org/wiki/Q220599","display_name":"Cytosol","level":3,"score":0.2793000042438507},{"id":"https://openalex.org/C65871279","wikidata":"https://www.wikidata.org/wiki/Q42884945","display_name":"Inner membrane","level":3,"score":0.2703999876976013},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.26600000262260437},{"id":"https://openalex.org/C155432671","wikidata":"https://www.wikidata.org/wiki/Q17073362","display_name":"Organelle biogenesis","level":4,"score":0.25760000944137573},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2506999969482422}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.01.755830","doi":"https://doi.org/10.64898/2026.10.01.755830","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.01.755830","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.01.755830.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.01.755830","doi":"https://doi.org/10.64898/2026.10.01.755830","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.01.755830","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.01.755830.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2891434558","display_name":null,"funder_award_id":"21H05147","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320310638","display_name":"McGill University","ror":"https://ror.org/01pxwe438"},{"id":"https://openalex.org/F4320311405","display_name":"Japan Agency for Medical Research and Development","ror":"https://ror.org/004rtk039"},{"id":"https://openalex.org/F4320320279","display_name":"University of Warwick","ror":"https://ror.org/01a77tt86"},{"id":"https://openalex.org/F4320321041","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784"},{"id":"https://openalex.org/F4320332603","display_name":"University of California, San Diego","ror":"https://ror.org/0168r3w48"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220865995.pdf","grobid_xml":"https://content.openalex.org/works/W7220865995.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1975709628","https://openalex.org/W2006229889","https://openalex.org/W2008417639","https://openalex.org/W2031172170","https://openalex.org/W2036787961","https://openalex.org/W2050346490","https://openalex.org/W2068623400","https://openalex.org/W2069025802","https://openalex.org/W2073672620","https://openalex.org/W2078527512","https://openalex.org/W2106507772","https://openalex.org/W2114847797","https://openalex.org/W2129247085","https://openalex.org/W2136000647","https://openalex.org/W2138722595","https://openalex.org/W2301499305","https://openalex.org/W2508534437","https://openalex.org/W2569101386","https://openalex.org/W2755192881","https://openalex.org/W2767606134","https://openalex.org/W2804617061","https://openalex.org/W2917864633","https://openalex.org/W2937251669","https://openalex.org/W2986770082","https://openalex.org/W3022527720","https://openalex.org/W3092810918","https://openalex.org/W3107492329","https://openalex.org/W3150928251","https://openalex.org/W4281741757","https://openalex.org/W4283260427","https://openalex.org/W4283593298","https://openalex.org/W4292283876","https://openalex.org/W4292284103","https://openalex.org/W4396810564","https://openalex.org/W4408305650","https://openalex.org/W4408362407","https://openalex.org/W4415085523","https://openalex.org/W7118844282"],"related_works":["https://openalex.org/W2569101386","https://openalex.org/W3092810918","https://openalex.org/W2320033110","https://openalex.org/W4397010195","https://openalex.org/W2099277984","https://openalex.org/W4387139419","https://openalex.org/W2094241924","https://openalex.org/W7217058504","https://openalex.org/W4298347324","https://openalex.org/W2810957190"],"abstract_inverted_index":{"Abstract":[0],"The":[1],"sarcoplasmic":[2],"reticulum":[3],"(SR)":[4],"is":[5,87],"an":[6,74],"elaborate":[7],"membrane":[8,158,172,180,197],"network":[9],"and":[10,23,32,50,93],"the":[11,35,102,116,150,175],"primary":[12],"intracellular":[13],"Ca":[14],"2+":[15],"store":[16],"for":[17,89],"muscle":[18,30,96,166,192,215],"contraction.":[19],"SR":[20,48,80,91,108,121,138,157,179,189,196,211],"abundance,":[21],"morphology,":[22],"spatial":[24],"organization":[25],"must":[26],"be":[27],"remodeled":[28],"during":[29,95,165,184,191,213],"atrophy":[31],"hypertrophy,":[33],"yet":[34],"mechanisms":[36],"underlying":[37],"this":[38,54,171],"remodeling":[39,212],"remain":[40],"poorly":[41],"understood.":[42],"Autophagy":[43],"has":[44],"been":[45],"implicated":[46],"in":[47],"homeostasis":[49],"may":[51],"contribute":[52],"to":[53,115,125,181,210],"process.":[55],"We":[56,168],"previously":[57],"showed":[58],"that":[59,85,156,170,205],"a":[60,142],"subset":[61],"of":[62,104,107,118,149,177],"Drosophila":[63,214],"larval":[64],"muscles":[65,72],"remodels":[66],"into":[67,162,201],"adult":[68],"dorsal":[69],"internal":[70],"oblique":[71],"through":[73],"autophagy-dependent":[75],"process":[76],"accompanied":[77],"by":[78],"extensive":[79],"reorganization.":[81],"Here,":[82],"we":[83],"show":[84],"autophagy":[86],"essential":[88],"maintaining":[90],"abundance":[92],"architecture":[94],"remodeling.":[97,167,193],"Blocking":[98],"autophagosome":[99,182,206],"formation":[100],"caused":[101],"accumulation":[103,117],"large":[105],"aggregates":[106],"membranes,":[109,203],"whereas":[110],"blocking":[111],"autophagosome\u2013lysosome":[112],"fusion":[113],"led":[114],"autophagosomes":[119],"with":[120],"transmembrane":[122],"proteins":[123],"localized":[124],"their":[126],"membranes.":[127],"Moreover,":[128],"disrupting":[129],"LC3-interacting":[130],"region":[131],"(LIR)-dependent":[132],"cargo":[133],"recognition":[134],"did":[135],"not":[136],"impair":[137],"remodeling,":[139],"arguing":[140],"against":[141],"major":[143],"contribution":[144],"from":[145],"selective":[146],"autophagic":[147],"degradation":[148],"SR.":[151],"Instead,":[152],"our":[153],"findings":[154],"suggest":[155],"components":[159,198],"are":[160,199],"incorporated":[161,200],"autophagosomal":[163,202],"membranes":[164],"propose":[169],"flow":[173],"couples":[174],"removal":[176],"excess":[178],"biogenesis":[183,207],"atrophy,":[185],"thereby":[186],"facilitating":[187],"large-scale":[188],"reorganization":[190],"Summary":[194],"statement":[195],"suggesting":[204],"directly":[208],"contributes":[209],"metamorphosis.":[216]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-08T00:00:00"}