{"id":"https://openalex.org/W7220865736","doi":"https://doi.org/10.64898/2026.10.06.757176","title":"Functional characterization of terpene synthases from Dendrobium nobile provides insights into the biosynthesis of copaborneol, a proposed dendrobine precursor","display_name":"Functional characterization of terpene synthases from Dendrobium nobile provides insights into the biosynthesis of copaborneol, a proposed dendrobine precursor","publication_year":2026,"publication_date":"2026-10-07","ids":{"openalex":"https://openalex.org/W7220865736","doi":"https://doi.org/10.64898/2026.10.06.757176"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.06.757176","doi":"https://doi.org/10.64898/2026.10.06.757176","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.757176","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757176.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757176.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5124074450","display_name":"Dandan Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Dandan Li","raw_affiliation_strings":["Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China","Guizhou Key Laboratory for Germplasm Innovation and Resource-Efficient Utilization of Dao-di Herbs, Huaxi 550025, Guiyang, Guizhou, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Key Laboratory for Germplasm Innovation and Resource-Efficient Utilization of Dao-di Herbs, Huaxi 550025, Guiyang, Guizhou, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101025714","display_name":"Wang Jianling","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianling Wang","raw_affiliation_strings":["Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157812487","display_name":"Sijing Fu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Sijing Fu","raw_affiliation_strings":["Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China","institution_ids":["https://openalex.org/I178232147"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101449308","display_name":"Hualei Wang","orcid":"https://orcid.org/0000-0001-8762-0189","observed_orcids":["https://orcid.org/0000-0001-8762-0189"]},"institutions":[{"id":"https://openalex.org/I178232147","display_name":"Guizhou University","ror":"https://ror.org/02wmsc916","country_code":"CN","type":"education","lineage":["https://openalex.org/I178232147"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hualei Wang","raw_affiliation_strings":["Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China","Guizhou Key Laboratory for Germplasm Innovation and Resource-Efficient Utilization of Dao-di Herbs, Huaxi 550025, Guiyang, Guizhou, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Agronomy College, Guizhou University, Huaxi 550025, Guiyang, Guizhou, P. R. China","institution_ids":["https://openalex.org/I178232147"]},{"raw_affiliation_string":"Guizhou Key Laboratory for Germplasm Innovation and Resource-Efficient Utilization of Dao-di Herbs, Huaxi 550025, Guiyang, Guizhou, China","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5124074450"],"corresponding_institution_ids":["https://openalex.org/I178232147"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11237","display_name":"Plant Gene Expression Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/terpene-synthase","display_name":"terpene synthase","score":0.9869999885559082},{"id":"https://openalex.org/keywords/dendrobium-nobile","display_name":"Dendrobium nobile","score":0.9539999961853027},{"id":"https://openalex.org/keywords/sesquiterpene-biosynthesis","display_name":"sesquiterpene biosynthesis","score":0.8389999866485596},{"id":"https://openalex.org/keywords/product-selectivity","display_name":"product selectivity","score":0.6610000133514404},{"id":"https://openalex.org/keywords/cytosolic-enzyme-localization","display_name":"cytosolic enzyme localization","score":0.734000027179718},{"id":"https://openalex.org/keywords/phylogenetic-analysis","display_name":"phylogenetic analysis","score":0.6669999957084656},{"id":"https://openalex.org/keywords/dendrobium","display_name":"Dendrobium","score":0.609000027179718}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6464999914169312},{"id":"https://openalex.org/C2776211264","wikidata":"https://www.wikidata.org/wiki/Q80218","display_name":"Sesquiterpene","level":2,"score":0.636900007724762},{"id":"https://openalex.org/C44937612","wikidata":"https://www.wikidata.org/wiki/Q212364","display_name":"Terpene","level":2,"score":0.6298999786376953},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.5113000273704529},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4828000068664551},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.43970000743865967},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.436599999666214},{"id":"https://openalex.org/C2780562374","wikidata":"https://www.wikidata.org/wiki/Q3024956","display_name":"Nicotiana benthamiana","level":3,"score":0.42149999737739563},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.39309999346733093},{"id":"https://openalex.org/C2779396153","wikidata":"https://www.wikidata.org/wiki/Q901227","display_name":"Natural product","level":2,"score":0.3763999938964844},{"id":"https://openalex.org/C2777347573","wikidata":"https://www.wikidata.org/wiki/Q522244","display_name":"Secondary metabolite","level":3,"score":0.36489999294281006},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.362199991941452},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3131999969482422},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.29679998755455017},{"id":"https://openalex.org/C123130949","wikidata":"https://www.wikidata.org/wiki/Q839659","display_name":"Phyllotaxis","level":4,"score":0.2953999936580658},{"id":"https://openalex.org/C2779268744","wikidata":"https://www.wikidata.org/wiki/Q407479","display_name":"Oxidoreductase","level":3,"score":0.2904999852180481},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.26739999651908875},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.2605000138282776},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.25440001487731934},{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.2540999948978424}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.06.757176","doi":"https://doi.org/10.64898/2026.10.06.757176","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.757176","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757176.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.06.757176","doi":"https://doi.org/10.64898/2026.10.06.757176","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.757176","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757176.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320323137","display_name":"Chinese Academy of Medical Sciences","ror":"https://ror.org/03cve4549"},{"id":"https://openalex.org/F4320325065","display_name":"Sichuan Agricultural University","ror":"https://ror.org/0388c3403"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220865736.pdf","grobid_xml":"https://content.openalex.org/works/W7220865736.grobid-xml"},"referenced_works_count":28,"referenced_works":["https://openalex.org/W1709815505","https://openalex.org/W1972529692","https://openalex.org/W2004115102","https://openalex.org/W2027711596","https://openalex.org/W2032653436","https://openalex.org/W2050293154","https://openalex.org/W2086323893","https://openalex.org/W2092660173","https://openalex.org/W2124645007","https://openalex.org/W2149430024","https://openalex.org/W2160378127","https://openalex.org/W2322928702","https://openalex.org/W2750432728","https://openalex.org/W2971082441","https://openalex.org/W3005235420","https://openalex.org/W3107541694","https://openalex.org/W3153024859","https://openalex.org/W4244736178","https://openalex.org/W4281254848","https://openalex.org/W4295359176","https://openalex.org/W4295903621","https://openalex.org/W4322771316","https://openalex.org/W4385141999","https://openalex.org/W4386968677","https://openalex.org/W4391611694","https://openalex.org/W4392023236","https://openalex.org/W4413983735","https://openalex.org/W7165025631"],"related_works":["https://openalex.org/W4407373908","https://openalex.org/W4302424839","https://openalex.org/W4281888835","https://openalex.org/W4392023236","https://openalex.org/W4321217393","https://openalex.org/W4410597987","https://openalex.org/W2774636479","https://openalex.org/W4401512853","https://openalex.org/W4406021336","https://openalex.org/W4415305323"],"abstract_inverted_index":{"Abstract":[0],"Picrotoxane-type":[1],"sesquiterpene":[2,158],"alkaloids,":[3],"including":[4],"dendrobine,":[5],"are":[6],"characteristic":[7],"constituents":[8],"of":[9,37,58,97,114,131,157,180],"Dendrobium":[10,182],"nobile":[11,190],",":[12,87],"but":[13],"the":[14,59,154,168,208],"enzymes":[15],"that":[16,76,147,187,199],"produce":[17],"their":[18,91],"proposed":[19,209],"skeletal":[20],"precursors":[21],"remain":[22],"incompletely":[23],"characterized.":[24],"Here,":[25],"we":[26,140],"combined":[27],"tissue":[28],"metabolite":[29],"profiling":[30],"with":[31],"genome-guided":[32],"screening":[33,68],"and":[34,48,53,101,106,123,137,145,151,161,171,195],"biochemical":[35],"characterization":[36],"terpene":[38],"synthases":[39],"(TPSs).":[40],"GC-MS":[41],"analysis":[42],"revealed":[43],"variation":[44],"in":[45,69,120,167],"sesquiterpenoid":[46],"profiles":[47],"dendrobine":[49,212],"signals":[50],"among":[51],"tissues":[52],"developmental":[54],"stages.":[55],"Manual":[56],"curation":[57],"chromosome-level":[60],"genome":[61],"yielded":[62],"16":[63],"full-length":[64],"TPS-a":[65],"candidates.":[66],"Functional":[67],"an":[70],"FPP-supplying":[71],"Escherichia":[72],"coli":[73],"system":[74],"showed":[75],"three":[77,188],"closely":[78],"related":[79,173],"genes":[80],"on":[81],"chromosome":[82],"1,":[83],"DnTPS1":[84,98,143,197],"\u2013":[85],"DnTPS3":[86],"produced":[88],"copaborneol":[89,194],"as":[90],"dominant":[92],"product.":[93],"The":[94],"principal":[95],"product":[96,201],"was":[99],"purified":[100],"identified":[102,172],"by":[103,134],"NMR":[104],"spectroscopy,":[105],"a":[107,204],"purified-enzyme":[108],"assay":[109],"confirmed":[110],"its":[111],"direct":[112],"conversion":[113],"FPP":[115],"to":[116,211],"copaborneol.":[117],"Fluorescence":[118],"imaging":[119],"rice":[121],"protoplasts":[122],"Nicotiana":[124],"benthamiana":[125],"leaves":[126],"indicated":[127],"predominantly":[128],"cytosolic":[129],"localization":[130],"DnTPS1.":[132],"Guided":[133],"sequence":[135],"comparisons":[136],"structural":[138],"modeling,":[139],"tested":[141],"12":[142],"variants":[144],"found":[146],"substitutions":[148],"at":[149],"W377":[150],"I295":[152],"altered":[153],"relative":[155],"distribution":[156],"products.":[159],"Phylogenetic":[160],"regional":[162],"gene-order":[163],"analyses":[164],"placed":[165],"DnTPS1\u223cDnTPS3":[166],"same":[169],"clade":[170],"TPS":[174],"candidates":[175],"within":[176],"corresponding":[177],"genomic":[178],"regions":[179],"six":[181],"species.":[183],"These":[184],"findings":[185],"establish":[186],"D.":[189],"TPSs":[191],"can":[192],"form":[193],"identify":[196],"residues":[198],"influence":[200],"selectivity,":[202],"providing":[203],"basis":[205],"for":[206],"testing":[207],"connection":[210],"biosynthesis.":[213]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-08T00:00:00"}