{"id":"https://openalex.org/W7220846264","doi":"https://doi.org/10.64898/2026.10.07.757222","title":"Engineering developmental transitions during human neurulation through modular integration of multimodal spatial cues","display_name":"Engineering developmental transitions during human neurulation through modular integration of multimodal spatial cues","publication_year":2026,"publication_date":"2026-10-07","ids":{"openalex":"https://openalex.org/W7220846264","doi":"https://doi.org/10.64898/2026.10.07.757222"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.07.757222","doi":"https://doi.org/10.64898/2026.10.07.757222","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.07.757222","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.07.757222.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.07.757222.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083862632","display_name":"Masaya HAGIWARA","orcid":"https://orcid.org/0000-0002-9032-4628","observed_orcids":["https://orcid.org/0000-0002-9032-4628"]},"institutions":[{"id":"https://openalex.org/I4210116810","display_name":"RIKEN Center for Biosystems Dynamics Research","ror":"https://ror.org/023rffy11","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652","https://openalex.org/I4210116810"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Masaya Hagiwara","raw_affiliation_strings":["Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047"],"raw_orcid":"https://orcid.org/0000-0002-9032-4628","affiliations":[{"raw_affiliation_string":"Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047","institution_ids":["https://openalex.org/I4210116810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032155551","display_name":"Sae Nozaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210116810","display_name":"RIKEN Center for Biosystems Dynamics Research","ror":"https://ror.org/023rffy11","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652","https://openalex.org/I4210116810"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Sae Nozaki","raw_affiliation_strings":["Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047","institution_ids":["https://openalex.org/I4210116810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077822469","display_name":"Yosuke SUGINO","orcid":"https://orcid.org/0009-0008-7482-9686","observed_orcids":["https://orcid.org/0009-0008-7482-9686"]},"institutions":[{"id":"https://openalex.org/I4210116810","display_name":"RIKEN Center for Biosystems Dynamics Research","ror":"https://ror.org/023rffy11","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652","https://openalex.org/I4210116810"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yosuke Sugino","raw_affiliation_strings":["Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047"],"raw_orcid":"https://orcid.org/0009-0008-7482-9686","affiliations":[{"raw_affiliation_string":"Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047","institution_ids":["https://openalex.org/I4210116810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089570985","display_name":"Yen Xuan Ngo","orcid":"https://orcid.org/0000-0003-2263-4509","observed_orcids":["https://orcid.org/0000-0003-2263-4509"]},"institutions":[{"id":"https://openalex.org/I4210116810","display_name":"RIKEN Center for Biosystems Dynamics Research","ror":"https://ror.org/023rffy11","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652","https://openalex.org/I4210116810"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yen Xuan Ngo","raw_affiliation_strings":["Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047"],"raw_orcid":"https://orcid.org/0000-0003-2263-4509","affiliations":[{"raw_affiliation_string":"Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047","institution_ids":["https://openalex.org/I4210116810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033284973","display_name":"Laura Yuriko Gonz\u00e1lez-Teshima","orcid":"https://orcid.org/0000-0003-1816-9771","observed_orcids":["https://orcid.org/0000-0003-1816-9771"]},"institutions":[{"id":"https://openalex.org/I4210116810","display_name":"RIKEN Center for Biosystems Dynamics Research","ror":"https://ror.org/023rffy11","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652","https://openalex.org/I4210116810"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Laura Yuriko Gonzalez-Teshima","raw_affiliation_strings":["Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047"],"raw_orcid":"https://orcid.org/0000-0003-1816-9771","affiliations":[{"raw_affiliation_string":"Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan 650-0047","institution_ids":["https://openalex.org/I4210116810"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070185488","display_name":"Isao Matsuo","orcid":"https://orcid.org/0000-0002-5916-6246","observed_orcids":["https://orcid.org/0000-0002-5916-6246"]},"institutions":[{"id":"https://openalex.org/I4210095711","display_name":"Osaka Women's and Children's Hospital","ror":"https://ror.org/00nx7n658","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210095711","https://openalex.org/I4405282797"]},{"id":"https://openalex.org/I4405282797","display_name":"Osaka Prefectural Hospital Organization","ror":"https://ror.org/00fb90w44","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4405282797"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Isao Matsuo","raw_affiliation_strings":["Department of Molecular Embryology, Research Institute, Osaka Women\u2019s and Children\u2019s Hospital, Osaka Prefectural Hospital Organization, Izumi Osaka 594-1101"],"raw_orcid":"https://orcid.org/0000-0002-5916-6246","affiliations":[{"raw_affiliation_string":"Department of Molecular Embryology, Research Institute, Osaka Women\u2019s and Children\u2019s Hospital, Osaka Prefectural Hospital Organization, Izumi Osaka 594-1101","institution_ids":["https://openalex.org/I4210095711","https://openalex.org/I4405282797"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5083862632"],"corresponding_institution_ids":["https://openalex.org/I4210116810"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.701200008392334,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.701200008392334,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.19509999454021454,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.025599999353289604,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neural-tube-closure","display_name":"neural tube closure","score":0.949999988079071},{"id":"https://openalex.org/keywords/induced-pluripotent-stem-cells","display_name":"induced pluripotent stem cells","score":0.800000011920929},{"id":"https://openalex.org/keywords/morphogen-gradients","display_name":"morphogen gradients","score":0.8980000019073486},{"id":"https://openalex.org/keywords/neural-induction","display_name":"neural induction","score":0.703000009059906},{"id":"https://openalex.org/keywords/dorsoventral-patterning","display_name":"dorsoventral patterning","score":0.8360000252723694},{"id":"https://openalex.org/keywords/bifurcation","display_name":"bifurcation","score":0.6290000081062317}],"concepts":[{"id":"https://openalex.org/C2778386714","wikidata":"https://www.wikidata.org/wiki/Q417084","display_name":"Morphogen","level":3,"score":0.8303999900817871},{"id":"https://openalex.org/C101468663","wikidata":"https://www.wikidata.org/wiki/Q1620158","display_name":"Modular design","level":2,"score":0.6216999888420105},{"id":"https://openalex.org/C102225024","wikidata":"https://www.wikidata.org/wiki/Q1388375","display_name":"Neurulation","level":5,"score":0.572700023651123},{"id":"https://openalex.org/C2780594696","wikidata":"https://www.wikidata.org/wiki/Q1127402","display_name":"Neural tube","level":3,"score":0.5554999709129333},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5259000062942505},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.4562000036239624},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.41179999709129333},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.38830000162124634},{"id":"https://openalex.org/C2778500370","wikidata":"https://www.wikidata.org/wiki/Q212344","display_name":"Inner ear","level":2,"score":0.382099986076355},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.3799000084400177},{"id":"https://openalex.org/C103607123","wikidata":"https://www.wikidata.org/wiki/Q7002059","display_name":"Neural fold","level":5,"score":0.35690000653266907},{"id":"https://openalex.org/C51929080","wikidata":"https://www.wikidata.org/wiki/Q2425187","display_name":"Codebase","level":3,"score":0.3540000021457672},{"id":"https://openalex.org/C30503417","wikidata":"https://www.wikidata.org/wiki/Q958938","display_name":"Organogenesis","level":3,"score":0.35120001435279846},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.34860000014305115},{"id":"https://openalex.org/C2776303644","wikidata":"https://www.wikidata.org/wiki/Q1020499","display_name":"Interfacing","level":2,"score":0.33880001306533813},{"id":"https://openalex.org/C197849852","wikidata":"https://www.wikidata.org/wiki/Q2314823","display_name":"Neural plate","level":4,"score":0.31209999322891235},{"id":"https://openalex.org/C29648211","wikidata":"https://www.wikidata.org/wiki/Q1995607","display_name":"Directionality","level":2,"score":0.2996000051498413},{"id":"https://openalex.org/C187761678","wikidata":"https://www.wikidata.org/wiki/Q7002068","display_name":"Neural tissue engineering","level":3,"score":0.29840001463890076},{"id":"https://openalex.org/C10854531","wikidata":"https://www.wikidata.org/wiki/Q1061415","display_name":"Regenerative medicine","level":3,"score":0.2922999858856201},{"id":"https://openalex.org/C135073763","wikidata":"https://www.wikidata.org/wiki/Q1271432","display_name":"Neuroectoderm","level":5,"score":0.2903999984264374},{"id":"https://openalex.org/C49892992","wikidata":"https://www.wikidata.org/wiki/Q1540285","display_name":"Tissue engineering","level":2,"score":0.2892000079154968},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.2865000069141388},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.2770000100135803},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.27000001072883606},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.2644999921321869},{"id":"https://openalex.org/C2986265897","wikidata":"https://www.wikidata.org/wiki/Q23852","display_name":"Human physiology","level":2,"score":0.25110000371932983}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.07.757222","doi":"https://doi.org/10.64898/2026.10.07.757222","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.07.757222","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.07.757222.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.07.757222","doi":"https://doi.org/10.64898/2026.10.07.757222","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.07.757222","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.07.757222.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3917221753","display_name":"Development of an In Vitro Disease Model Incorporating Organ-Organ Interaction","funder_award_id":"24H00806","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320311405","display_name":"Japan Agency for Medical Research and Development","ror":"https://ror.org/004rtk039"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220846264.pdf","grobid_xml":"https://content.openalex.org/works/W7220846264.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1163662100","https://openalex.org/W2411867197","https://openalex.org/W3208361136","https://openalex.org/W3210728577","https://openalex.org/W3095009105","https://openalex.org/W3007798095","https://openalex.org/W3146618265","https://openalex.org/W2804583160","https://openalex.org/W2127004672","https://openalex.org/W4389682132"],"abstract_inverted_index":{"Abstract":[0],"Reconstituting":[1],"organ":[2],"development":[3],"in":[4],"vitro":[5],"requires":[6],"reproducing":[7],"dynamic":[8],"developmental":[9],"transitions":[10],"rather":[11],"than":[12],"only":[13],"terminal":[14],"tissue":[15,57,87,116],"structures.":[16],"Here,":[17],"we":[18],"developed":[19,124],"MoSAIC":[20,52,132],"(Modular":[21],"Spatial":[22],"Assembly":[23],"and":[24,36,64,72,89,93,139],"Integration":[25],"of":[26,56,99,129,137],"Cues),":[27],"a":[28,80,100],"modular":[29],"platform":[30],"that":[31],"decomposes":[32],"complex":[33],"culture":[34],"operations":[35],"subsequently":[37],"integrates":[38],"them":[39],"to":[40,142],"reconstruct":[41],"multimodal":[42],"mechano-chemical":[43],"spatial":[44],"cues.":[45],"Using":[46,122],"human":[47,130],"induced":[48],"pluripotent":[49],"stem":[50],"cells,":[51],"enabled":[53],"independent":[54],"control":[55],"geometry,":[58],"extracellular":[59],"matrix":[60],"localization,":[61],"physical":[62],"boundaries,":[63],"morphogen":[65,107],"gradients,":[66],"while":[67,113],"also":[68],"permitting":[69],"defined":[70],"mechanical":[71,138],"chemical":[73,140],"perturbations":[74],"during":[75],"development.":[76],"Their":[77],"integration":[78],"guided":[79],"continuous":[81],"ectodermal":[82],"sheet":[83],"through":[84],"neural":[85,92,102,119,147],"induction,":[86],"bending,":[88],"bifurcation":[90],"into":[91],"epidermal":[94],"tissues,":[95],"followed":[96],"by":[97],"formation":[98],"polarized":[101],"tube":[103,120,148],"structure.":[104],"Spatially":[105],"controlled":[106],"exposure":[108],"further":[109,133],"established":[110],"dorsoventral":[111],"patterning,":[112],"the":[114,123,127,144],"resulting":[115],"exhibited":[117],"posterior":[118],"identity.":[121],"model":[125],"recapitulating":[126],"transition":[128],"neurulation,":[131],"enables":[134],"non-genetic":[135],"perturbation":[136],"cues":[141],"interrogate":[143],"mechanisms":[145],"governing":[146],"closure.":[149]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-08T00:00:00"}