{"id":"https://openalex.org/W7220837224","doi":"https://doi.org/10.64898/2026.10.06.754922","title":"Human FACT complex coordinates cohesin and transcription on chromatin","display_name":"Human FACT complex coordinates cohesin and transcription on chromatin","publication_year":2026,"publication_date":"2026-10-07","ids":{"openalex":"https://openalex.org/W7220837224","doi":"https://doi.org/10.64898/2026.10.06.754922"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.06.754922","doi":"https://doi.org/10.64898/2026.10.06.754922","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.754922","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.06.754922.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.06.754922.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081669122","display_name":"Jonay Garc\u00eda-Luis","orcid":"https://orcid.org/0000-0002-2799-7491","observed_orcids":["https://orcid.org/0000-0002-2799-7491"]},"institutions":[{"id":"https://openalex.org/I158438070","display_name":"Universidad de La Laguna","ror":"https://ror.org/01r9z8p25","country_code":"ES","type":"education","lineage":["https://openalex.org/I158438070"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Jonay Garcia-Luis","raw_affiliation_strings":["Universidad de La Laguna;"],"raw_orcid":"https://orcid.org/0000-0002-2799-7491","affiliations":[{"raw_affiliation_string":"Universidad de La Laguna;","institution_ids":["https://openalex.org/I158438070"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111792857","display_name":"Andr\u00e9s Canela","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I22299242","display_name":"Kyoto University","ror":"https://ror.org/02kpeqv85","country_code":"JP","type":"education","lineage":["https://openalex.org/I22299242"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Andres Canela","raw_affiliation_strings":["Graduate School of Biostudies, Kyoto University, Kyoto, Japan;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Biostudies, Kyoto University, Kyoto, Japan;","institution_ids":["https://openalex.org/I22299242"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091493092","display_name":"Valeriya Malysheva","orcid":"https://orcid.org/0000-0002-0919-7845","observed_orcids":["https://orcid.org/0000-0002-0919-7845"]},"institutions":[{"id":"https://openalex.org/I4210132484","display_name":"VIB-UAntwerp Center for Molecular Neurology","ror":"https://ror.org/041x7eh14","country_code":"BE","type":"facility","lineage":["https://openalex.org/I149213910","https://openalex.org/I2802017950","https://openalex.org/I4210132484"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Valeriya Malysheva","raw_affiliation_strings":["VIB-UAntwerp Center for Molecular Neurology;"],"raw_orcid":"https://orcid.org/0000-0002-0919-7845","affiliations":[{"raw_affiliation_string":"VIB-UAntwerp Center for Molecular Neurology;","institution_ids":["https://openalex.org/I4210132484"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084819322","display_name":"Toyoaki Natsume","orcid":"https://orcid.org/0000-0002-3544-4491","observed_orcids":["https://orcid.org/0000-0002-3544-4491"]},"institutions":[{"id":"https://openalex.org/I4210098918","display_name":"Tokyo Metropolitan Institute of Medical Science","ror":"https://ror.org/00vya8493","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210098918"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toyoaki Natsume","raw_affiliation_strings":["Tokyo Metropolitan Institute of Medical Science;"],"raw_orcid":"https://orcid.org/0000-0002-3544-4491","affiliations":[{"raw_affiliation_string":"Tokyo Metropolitan Institute of Medical Science;","institution_ids":["https://openalex.org/I4210098918"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076209108","display_name":"Mohammad Mahdi Karimi","orcid":"https://orcid.org/0000-0001-5017-1252","observed_orcids":["https://orcid.org/0000-0001-5017-1252"]},"institutions":[{"id":"https://openalex.org/I183935753","display_name":"King's College London","ror":"https://ror.org/0220mzb33","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I183935753"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Mohammad M Karimi","raw_affiliation_strings":["King's College London;"],"raw_orcid":"https://orcid.org/0000-0001-5017-1252","affiliations":[{"raw_affiliation_string":"King's College London;","institution_ids":["https://openalex.org/I183935753"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020161951","display_name":"Masato T. Kanemaki","orcid":"https://orcid.org/0000-0002-7657-1649","observed_orcids":["https://orcid.org/0000-0002-7657-1649"]},"institutions":[{"id":"https://openalex.org/I113198587","display_name":"National Institute of Genetics","ror":"https://ror.org/02xg1m795","country_code":"JP","type":"facility","lineage":["https://openalex.org/I113198587","https://openalex.org/I1319490839","https://openalex.org/I4210158934"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masato Kanemaki","raw_affiliation_strings":["National Institute of Genetics;"],"raw_orcid":"https://orcid.org/0000-0002-7657-1649","affiliations":[{"raw_affiliation_string":"National Institute of Genetics;","institution_ids":["https://openalex.org/I113198587"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074679629","display_name":"Mikhail Spivakov","orcid":"https://orcid.org/0000-0002-0383-3943","observed_orcids":["https://orcid.org/0000-0002-0383-3943"]},"institutions":[{"id":"https://openalex.org/I4210161592","display_name":"MRC London Institute of Medical Sciences","ror":"https://ror.org/05p1n6x86","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210161592","https://openalex.org/I47508984"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Mikhail Spivakov","raw_affiliation_strings":["MRC Laboratory of Medical Sciences"],"raw_orcid":"https://orcid.org/0000-0002-0383-3943","affiliations":[{"raw_affiliation_string":"MRC Laboratory of Medical Sciences","institution_ids":["https://openalex.org/I4210161592","https://openalex.org/I90344618"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026968805","display_name":"Lu\u00eds Arag\u00f3n","orcid":"https://orcid.org/0000-0003-0634-6742","observed_orcids":["https://orcid.org/0000-0003-0634-6742","https://orcid.org/0000-0003-2787-9070"]},"institutions":[{"id":"https://openalex.org/I4210161592","display_name":"MRC London Institute of Medical Sciences","ror":"https://ror.org/05p1n6x86","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210161592","https://openalex.org/I47508984"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Luis Aragon","raw_affiliation_strings":["MRC Laboratory of Medical Sciences"],"raw_orcid":"https://orcid.org/0000-0003-0634-6742","affiliations":[{"raw_affiliation_string":"MRC Laboratory of Medical Sciences","institution_ids":["https://openalex.org/I4210161592","https://openalex.org/I90344618"]}]}],"institutions":[],"countries_distinct_count":4,"institutions_distinct_count":8,"corresponding_author_ids":["https://openalex.org/A5026968805"],"corresponding_institution_ids":["https://openalex.org/I4210161592","https://openalex.org/I90344618"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fact-complex","display_name":"FACT complex","score":0.9440000057220459},{"id":"https://openalex.org/keywords/cohesin","display_name":"cohesin","score":0.9810000061988831},{"id":"https://openalex.org/keywords/chromatin-looping","display_name":"chromatin looping","score":0.7099999785423279},{"id":"https://openalex.org/keywords/loop-extrusion","display_name":"loop extrusion","score":0.8109999895095825},{"id":"https://openalex.org/keywords/topologically-associated-domains","display_name":"topologically associating domains","score":0.9380000233650208},{"id":"https://openalex.org/keywords/chromatin-organization","display_name":"chromatin organization","score":0.5329999923706055},{"id":"https://openalex.org/keywords/transcription","display_name":"transcription","score":0.6549999713897705},{"id":"https://openalex.org/keywords/histone-chaperone","display_name":"histone chaperone","score":0.8180000185966492},{"id":"https://openalex.org/keywords/3d-genome-organization","display_name":"3D genome organization","score":0.699999988079071}],"concepts":[{"id":"https://openalex.org/C115821613","wikidata":"https://www.wikidata.org/wiki/Q21103063","display_name":"Cohesin","level":4,"score":0.9918000102043152},{"id":"https://openalex.org/C83640560","wikidata":"https://www.wikidata.org/wiki/Q180951","display_name":"Chromatin","level":3,"score":0.8320000171661377},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6464999914169312},{"id":"https://openalex.org/C166941617","wikidata":"https://www.wikidata.org/wiki/Q421708","display_name":"SWI/SNF","level":5,"score":0.5267000198364258},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48249998688697815},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.46630001068115234},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.439300000667572},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.4226999878883362},{"id":"https://openalex.org/C2778760011","wikidata":"https://www.wikidata.org/wiki/Q21163346","display_name":"CTCF","level":5,"score":0.4171000123023987},{"id":"https://openalex.org/C2778354886","wikidata":"https://www.wikidata.org/wiki/Q5113927","display_name":"Chromosome segregation","level":4,"score":0.41519999504089355},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.38830000162124634},{"id":"https://openalex.org/C167227067","wikidata":"https://www.wikidata.org/wiki/Q5113739","display_name":"Chromatin remodeling","level":4,"score":0.37310001254081726},{"id":"https://openalex.org/C138626823","wikidata":"https://www.wikidata.org/wiki/Q916504","display_name":"Chromosomal translocation","level":3,"score":0.36579999327659607},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3562000095844269},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.31049999594688416},{"id":"https://openalex.org/C152369539","wikidata":"https://www.wikidata.org/wiki/Q5399999","display_name":"Establishment of sister chromatid cohesion","level":5,"score":0.3073999881744385},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.2985999882221222},{"id":"https://openalex.org/C14519417","wikidata":"https://www.wikidata.org/wiki/Q5113740","display_name":"Chromatin structure remodeling (RSC) complex","level":5,"score":0.29019999504089355},{"id":"https://openalex.org/C137338518","wikidata":"https://www.wikidata.org/wiki/Q4919210","display_name":"Bivalent chromatin","level":5,"score":0.2827000021934509},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.2827000021934509},{"id":"https://openalex.org/C27153228","wikidata":"https://www.wikidata.org/wiki/Q3787274","display_name":"Transcriptional regulation","level":4,"score":0.2825999855995178},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.2506999969482422}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.06.754922","doi":"https://doi.org/10.64898/2026.10.06.754922","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.754922","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.06.754922.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.06.754922","doi":"https://doi.org/10.64898/2026.10.06.754922","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.754922","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.06.754922.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220837224.pdf","grobid_xml":"https://content.openalex.org/works/W7220837224.grobid-xml"},"referenced_works_count":56,"referenced_works":["https://openalex.org/W1971196679","https://openalex.org/W1972307085","https://openalex.org/W1978696228","https://openalex.org/W1980991323","https://openalex.org/W2002954217","https://openalex.org/W2015808098","https://openalex.org/W2028022357","https://openalex.org/W2067651011","https://openalex.org/W2080993511","https://openalex.org/W2090037139","https://openalex.org/W2092992881","https://openalex.org/W2097201830","https://openalex.org/W2108871359","https://openalex.org/W2117081447","https://openalex.org/W2126888219","https://openalex.org/W2170551349","https://openalex.org/W2171808845","https://openalex.org/W2185693210","https://openalex.org/W2296073596","https://openalex.org/W2310030657","https://openalex.org/W2338485081","https://openalex.org/W2401568148","https://openalex.org/W2487957429","https://openalex.org/W2543542605","https://openalex.org/W2550670895","https://openalex.org/W2610160407","https://openalex.org/W2614144400","https://openalex.org/W2737563286","https://openalex.org/W2761461280","https://openalex.org/W2775505403","https://openalex.org/W2782638879","https://openalex.org/W2800216985","https://openalex.org/W2808525707","https://openalex.org/W2809178041","https://openalex.org/W2888200508","https://openalex.org/W2888425844","https://openalex.org/W2898577086","https://openalex.org/W2899663357","https://openalex.org/W2922770922","https://openalex.org/W2951946598","https://openalex.org/W2977661777","https://openalex.org/W2977744221","https://openalex.org/W2989713781","https://openalex.org/W2990803388","https://openalex.org/W2991248607","https://openalex.org/W2998399076","https://openalex.org/W3094879970","https://openalex.org/W3102624934","https://openalex.org/W4221095693","https://openalex.org/W4280510947","https://openalex.org/W4281701961","https://openalex.org/W4306747313","https://openalex.org/W4323825363","https://openalex.org/W4389513565","https://openalex.org/W4396667339","https://openalex.org/W4399107395"],"related_works":["https://openalex.org/W2977661777","https://openalex.org/W2338716506","https://openalex.org/W7046272737","https://openalex.org/W2607008467","https://openalex.org/W7131723343","https://openalex.org/W2543542605","https://openalex.org/W2084897210","https://openalex.org/W3187294536","https://openalex.org/W4323825363","https://openalex.org/W2964274647"],"abstract_inverted_index":{"The":[0],"spatial":[1],"organisation":[2,21,179],"of":[3,50,71,89,106,126],"eukaryotic":[4],"genomes":[5],"is":[6,119],"critical":[7],"for":[8],"coordinating":[9],"DNA-dependent":[10],"processes":[11],"such":[12],"as":[13,47,94],"transcription":[14,181],"and":[15,64,85],"replication.":[16],"Cohesin":[17],"contributes":[18],"to":[19,81,176],"this":[20,139],"by":[22,96],"extruding":[23],"chromatin":[24,34,83,171],"loops;":[25],"however,":[26],"how":[27,170],"cohesin":[28,51,67,74,107,127,136,148,174],"achieves":[29],"processive":[30],"translocation":[31,75,149],"along":[32,150],"nucleosome-dense":[33],"in":[35,53,103,115,128,182],"vivo":[36],"remains":[37],"poorly":[38],"understood.":[39],"Here,":[40],"we":[41],"identify":[42],"the":[43,59,104],"histone":[44],"chaperone":[45],"FACT":[46,60,72,100,146],"a":[48],"regulator":[49],"dynamics":[52],"human":[54],"cells.":[55,184],"We":[56],"show":[57],"that":[58,111,135,145],"subunit":[61],"SUPT16H":[62],"colocalises":[63],"interacts":[65],"with":[66,121,180],"on":[68],"chromatin.":[69],"Depletion":[70],"impairs":[73],"from":[76],"its":[77,158],"loading":[78],"sites,":[79],"leading":[80],"reduced":[82,122],"looping":[84],"decreased":[86],"boundary":[87],"strength":[88],"topologically":[90],"associating":[91],"domains":[92],"(TADs),":[93],"revealed":[95],"Hi-C.":[97],"In":[98],"addition,":[99],"depletion":[101],"results":[102],"accumulation":[105,160],"within":[108,161],"gene":[109],"bodies":[110],"are":[112],"not":[113],"engaged":[114],"loop":[116,154],"formation,":[117],"which":[118],"associated":[120],"transcription.":[123],"Importantly,":[124],"degradation":[125],"FACT-depleted":[129],"cells":[130],"restores":[131],"transcriptional":[132],"output,":[133],"indicating":[134],"mislocalisation":[137],"underlies":[138],"effect.":[140],"Together,":[141],"these":[142],"findings":[143],"demonstrate":[144],"facilitates":[147],"chromatin,":[151],"promoting":[152],"efficient":[153],"extrusion":[155],"while":[156],"preventing":[157],"aberrant":[159],"transcribed":[162],"regions.":[163],"This":[164],"work":[165],"provides":[166],"mechanistic":[167],"insight":[168],"into":[169],"structure":[172],"modulates":[173],"function":[175],"coordinate":[177],"genome":[178],"mammalian":[183]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-08T00:00:00"}