{"id":"https://openalex.org/W7220741126","doi":"https://doi.org/10.64898/2026.10.06.757045","title":"A helper NLR monitors catalytic perturbation of a vesicle trafficking regulator targeted by pathogen effectors","display_name":"A helper NLR monitors catalytic perturbation of a vesicle trafficking regulator targeted by pathogen effectors","publication_year":2026,"publication_date":"2026-10-07","ids":{"openalex":"https://openalex.org/W7220741126","doi":"https://doi.org/10.64898/2026.10.06.757045"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.06.757045","doi":"https://doi.org/10.64898/2026.10.06.757045","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.757045","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757045.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757045.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5073982559","display_name":"Enoch Lok Him Yuen","orcid":"https://orcid.org/0000-0002-7933-0605","observed_orcids":["https://orcid.org/0000-0002-7933-0605"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Enoch Lok Him Yuen","raw_affiliation_strings":["Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0002-7933-0605","affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5134252986","display_name":"Nick Moritz Eilmann","orcid":"https://orcid.org/0009-0001-5291-8767","observed_orcids":["https://orcid.org/0009-0001-5291-8767"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Nick Eilmann","raw_affiliation_strings":["Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom"],"raw_orcid":"https://orcid.org/0009-0001-5291-8767","affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113253559","display_name":"Yijun Wang","orcid":"https://orcid.org/0009-0004-1190-0861","observed_orcids":["https://orcid.org/0009-0004-1190-0861"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Yijun Wang","raw_affiliation_strings":["Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom"],"raw_orcid":"https://orcid.org/0009-0004-1190-0861","affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016788094","display_name":"Tolga O. Bozkurt","orcid":"https://orcid.org/0000-0003-0507-6875","observed_orcids":["https://orcid.org/0000-0003-0507-6875"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Tolga O. Bozkurt","raw_affiliation_strings":["Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0003-0507-6875","affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom","institution_ids":["https://openalex.org/I47508984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5016788094"],"corresponding_institution_ids":["https://openalex.org/I47508984"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.9404000043869019,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.9404000043869019,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11771","display_name":"Plant Pathogens and Resistance","score":0.03539999946951866,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nlr-receptors","display_name":"NLR immune receptors","score":0.7480000257492065},{"id":"https://openalex.org/keywords/rab-gtpase-activating-protein","display_name":"Rab GTPase-activating protein","score":0.925000011920929},{"id":"https://openalex.org/keywords/plant-immunity","display_name":"plant immunity","score":0.6159999966621399},{"id":"https://openalex.org/keywords/vesicular-trafficking","display_name":"vesicle trafficking","score":0.7699999809265137},{"id":"https://openalex.org/keywords/pathogen-effectors","display_name":"pathogen effectors","score":0.7870000004768372},{"id":"https://openalex.org/keywords/tiki","display_name":"tiki","score":0.9580000042915344},{"id":"https://openalex.org/keywords/susceptibility-factors","display_name":"susceptibility factors","score":0.5490000247955322},{"id":"https://openalex.org/keywords/plant-pathogen-interaction","display_name":"plant-pathogen interaction","score":0.578000009059906},{"id":"https://openalex.org/keywords/immunosurveillance","display_name":"immune surveillance","score":0.4779999852180481}],"concepts":[{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.9114999771118164},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.7429999709129333},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.7168999910354614},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6269999742507935},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5394999980926514},{"id":"https://openalex.org/C3017707949","wikidata":"https://www.wikidata.org/wiki/Q2138797","display_name":"Negative regulator","level":3,"score":0.5232999920845032},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.5095999836921692},{"id":"https://openalex.org/C3017607883","wikidata":"https://www.wikidata.org/wiki/Q25325354","display_name":"Master regulator","level":4,"score":0.4138000011444092},{"id":"https://openalex.org/C177918212","wikidata":"https://www.wikidata.org/wiki/Q803623","display_name":"Perturbation (astronomy)","level":2,"score":0.38339999318122864},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31049999594688416},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.27799999713897705},{"id":"https://openalex.org/C2779341262","wikidata":"https://www.wikidata.org/wiki/Q182581","display_name":"Immunity","level":3,"score":0.2619999945163727},{"id":"https://openalex.org/C177264268","wikidata":"https://www.wikidata.org/wiki/Q1514741","display_name":"Set (abstract data type)","level":2,"score":0.2515000104904175}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.10.06.757045","doi":"https://doi.org/10.64898/2026.10.06.757045","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.757045","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757045.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"doi:10.6084/m9.figshare.34113588","doi":"https://doi.org/10.6084/m9.figshare.34113588","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34113588","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"},{"id":"doi:10.6084/m9.figshare.34113588.v1","doi":"https://doi.org/10.6084/m9.figshare.34113588.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34113588.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.64898/2026.10.06.757045","doi":"https://doi.org/10.64898/2026.10.06.757045","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.06.757045","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/07/2026.10.06.757045.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320283","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220741126.pdf","grobid_xml":"https://content.openalex.org/works/W7220741126.grobid-xml"},"referenced_works_count":49,"referenced_works":["https://openalex.org/W1502336081","https://openalex.org/W1964061865","https://openalex.org/W1979777047","https://openalex.org/W2017048824","https://openalex.org/W2043661964","https://openalex.org/W2087157154","https://openalex.org/W2102370923","https://openalex.org/W2103936125","https://openalex.org/W2138946332","https://openalex.org/W2140673705","https://openalex.org/W2154540205","https://openalex.org/W2236302923","https://openalex.org/W2725106132","https://openalex.org/W2736049245","https://openalex.org/W2802192337","https://openalex.org/W2808859224","https://openalex.org/W2924139971","https://openalex.org/W2960945633","https://openalex.org/W2969721169","https://openalex.org/W2988942662","https://openalex.org/W2990575322","https://openalex.org/W3010470639","https://openalex.org/W3106866272","https://openalex.org/W3133707857","https://openalex.org/W3177828909","https://openalex.org/W3182631359","https://openalex.org/W3193924706","https://openalex.org/W3194995283","https://openalex.org/W4210962045","https://openalex.org/W4281790889","https://openalex.org/W4296802427","https://openalex.org/W4297243353","https://openalex.org/W4313280753","https://openalex.org/W4367856209","https://openalex.org/W4376106099","https://openalex.org/W4376602477","https://openalex.org/W4388969475","https://openalex.org/W4395469472","https://openalex.org/W4395666709","https://openalex.org/W4396721167","https://openalex.org/W4401216147","https://openalex.org/W4403114824","https://openalex.org/W4403520961","https://openalex.org/W4414011172","https://openalex.org/W7138898247","https://openalex.org/W7143519455","https://openalex.org/W7147188543","https://openalex.org/W7163641836","https://openalex.org/W7167066731"],"related_works":["https://openalex.org/W3194995283","https://openalex.org/W4400736105","https://openalex.org/W4410166548","https://openalex.org/W2580724658","https://openalex.org/W3005793791","https://openalex.org/W3193739344","https://openalex.org/W4283264288","https://openalex.org/W4390681706","https://openalex.org/W4313424363","https://openalex.org/W4387665731"],"abstract_inverted_index":{"Abstract":[0],"Plants":[1],"rely":[2],"on":[3],"vesicle":[4],"trafficking":[5,21],"for":[6],"immunity,":[7],"yet":[8],"pathogens":[9,141],"deploy":[10],"effectors":[11,65,115],"to":[12,53,95,133,144],"reprogram":[13],"these":[14,34],"pathways.":[15],"NLR":[16,59],"immune":[17,54,124],"receptors":[18],"can":[19,142],"monitor":[20],"components,":[22],"but":[23],"how":[24],"plants":[25,132],"detect":[26,134],"effector":[27,51],"perturbation":[28],"of":[29,75,136],"the":[30,57,71,109,114],"enzymatic":[31],"regulators":[32],"controlling":[33],"pathways":[35],"remains":[36],"unclear.":[37],"Here,":[38],"we":[39],"show":[40],"that":[41,113,128,140],"TOPGAP,":[42],"a":[43,137],"Rab":[44],"GTPase-activating":[45],"protein":[46],"and":[47,62,77,83,106],"susceptibility":[48],"factor,":[49],"couples":[50],"activity":[52,122],"signaling":[55],"involving":[56],"helper":[58],"NRC4.":[60],"PiE355":[61],"TIKI,":[63],"related":[64],"from":[66],"two":[67],"Phytophthora":[68],"species,":[69],"bind":[70],"N-terminal":[72,110],"regulatory":[73,111],"domain":[74,91,112],"TOPGAP":[76,82,89,121],"trigger":[78],"cell":[79,98],"death":[80],"requiring":[81],"its":[84],"C-terminal":[85],"GAP":[86],"activity.":[87],"The":[88],"catalytic":[90,104],"alone":[92],"is":[93,101],"sufficient":[94],"activate":[96],"NRC4-mediated":[97],"death,":[99],"which":[100],"abolished":[102],"by":[103,108],"inactivation":[105],"suppressed":[107],"bind.":[116],"These":[117],"findings":[118],"implicate":[119],"deregulated":[120],"in":[123],"activation.":[125],"We":[126],"propose":[127],"NRC4-dependent":[129],"surveillance":[130],"enables":[131],"deregulation":[135],"host":[138],"enzyme":[139],"co-opt":[143],"promote":[145],"infection.":[146]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-07T00:00:00"}
