{"id":"https://openalex.org/W7220719328","doi":"https://doi.org/10.64898/2026.10.05.756609","title":"RBM25 exhibits distinct RNA- and chromatin-associated target programs in human embryonic stem cells","display_name":"RBM25 exhibits distinct RNA- and chromatin-associated target programs in human embryonic stem cells","publication_year":2026,"publication_date":"2026-10-06","ids":{"openalex":"https://openalex.org/W7220719328","doi":"https://doi.org/10.64898/2026.10.05.756609"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.05.756609","doi":"https://doi.org/10.64898/2026.10.05.756609","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.05.756609","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.05.756609.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.05.756609.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102632153","display_name":"Yuanpu Sun","orcid":"https://orcid.org/0009-0001-8422-4778","observed_orcids":["https://orcid.org/0009-0001-8422-4778"]},"institutions":[{"id":"https://openalex.org/I174306211","display_name":"Technion \u2013 Israel Institute of Technology","ror":"https://ror.org/03qryx823","country_code":"IL","type":"education","lineage":["https://openalex.org/I174306211"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Yuanpu Sun","raw_affiliation_strings":["Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel"],"raw_orcid":"https://orcid.org/0009-0001-8422-4778","affiliations":[{"raw_affiliation_string":"Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel","institution_ids":["https://openalex.org/I174306211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000010095","display_name":"Tamar Lahav","orcid":"https://orcid.org/0009-0001-6756-6135","observed_orcids":["https://orcid.org/0009-0001-6756-6135"]},"institutions":[{"id":"https://openalex.org/I174306211","display_name":"Technion \u2013 Israel Institute of Technology","ror":"https://ror.org/03qryx823","country_code":"IL","type":"education","lineage":["https://openalex.org/I174306211"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Tamar Lahav","raw_affiliation_strings":["Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel","institution_ids":["https://openalex.org/I174306211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110446508","display_name":"Fabian Glaser","orcid":"https://orcid.org/0000-0001-8416-5416","observed_orcids":["https://orcid.org/0000-0001-8416-5416","https://orcid.org/0000-0003-4038-7185"]},"institutions":[{"id":"https://openalex.org/I174306211","display_name":"Technion \u2013 Israel Institute of Technology","ror":"https://ror.org/03qryx823","country_code":"IL","type":"education","lineage":["https://openalex.org/I174306211"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Fabian Glaser","raw_affiliation_strings":["Technion Human Health Initiative - Israel Institute of Technology, Faculty of Biology, Haifa, Israel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Technion Human Health Initiative - Israel Institute of Technology, Faculty of Biology, Haifa, Israel","institution_ids":["https://openalex.org/I174306211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016889739","display_name":"Tamar Hashimshony","orcid":"https://orcid.org/0000-0002-4786-838X","observed_orcids":["https://orcid.org/0000-0002-4786-838X"]},"institutions":[{"id":"https://openalex.org/I174306211","display_name":"Technion \u2013 Israel Institute of Technology","ror":"https://ror.org/03qryx823","country_code":"IL","type":"education","lineage":["https://openalex.org/I174306211"]}],"countries":["IL"],"is_corresponding":false,"raw_author_name":"Tamar Hashimshony","raw_affiliation_strings":["Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel"],"raw_orcid":"https://orcid.org/0000-0002-4786-838X","affiliations":[{"raw_affiliation_string":"Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel","institution_ids":["https://openalex.org/I174306211"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071783473","display_name":"Yael Mandel\u2010Gutfreund","orcid":"https://orcid.org/0000-0002-8946-8237","observed_orcids":["https://orcid.org/0000-0002-8946-8237"]},"institutions":[{"id":"https://openalex.org/I174306211","display_name":"Technion \u2013 Israel Institute of Technology","ror":"https://ror.org/03qryx823","country_code":"IL","type":"education","lineage":["https://openalex.org/I174306211"]}],"countries":["IL"],"is_corresponding":true,"raw_author_name":"Yael Mandel-Gutfreund","raw_affiliation_strings":["Technion- Israel Institute of Technology, Faculty of Computer Science, Haifa, Israel","Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel"],"raw_orcid":"https://orcid.org/0000-0002-8946-8237","affiliations":[{"raw_affiliation_string":"Technion- Israel Institute of Technology, Faculty of Computer Science, Haifa, Israel","institution_ids":["https://openalex.org/I174306211"]},{"raw_affiliation_string":"Technion-Israel Institute of Technology, Faculty of Biology, Haifa, Israel","institution_ids":["https://openalex.org/I174306211"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5071783473"],"corresponding_institution_ids":["https://openalex.org/I174306211"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.5170999765396118,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.5170999765396118,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.4350999891757965,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.013000000268220901,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-binding-proteins","display_name":"RNA-binding proteins","score":0.9890000224113464},{"id":"https://openalex.org/keywords/human-embryonic-stem-cells","display_name":"human embryonic stem cells","score":0.9120000004768372},{"id":"https://openalex.org/keywords/chromatin-binding","display_name":"chromatin binding","score":0.7480000257492065},{"id":"https://openalex.org/keywords/rna-splicing","display_name":"RNA splicing","score":0.6269999742507935},{"id":"https://openalex.org/keywords/post-transcriptional-regulation","display_name":"post-transcriptional regulation","score":0.8889999985694885},{"id":"https://openalex.org/keywords/cell-cycle-genes","display_name":"cell cycle genes","score":0.7110000252723694},{"id":"https://openalex.org/keywords/neuronal-gene-expression","display_name":"neuronal gene expression","score":0.5590000152587891}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.765999972820282},{"id":"https://openalex.org/C83640560","wikidata":"https://www.wikidata.org/wiki/Q180951","display_name":"Chromatin","level":3,"score":0.727400004863739},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.6263999938964844},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.5979999899864197},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.5333999991416931},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5156000256538391},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5110999941825867},{"id":"https://openalex.org/C94671646","wikidata":"https://www.wikidata.org/wiki/Q207551","display_name":"Intron","level":3,"score":0.4880000054836273},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.45750001072883606},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.45239999890327454},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.3953999876976013},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.37860000133514404},{"id":"https://openalex.org/C41282012","wikidata":"https://www.wikidata.org/wiki/Q3928341","display_name":"RNA-binding protein","level":4,"score":0.35519999265670776},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.3458000123500824},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.3212999999523163},{"id":"https://openalex.org/C134320426","wikidata":"https://www.wikidata.org/wiki/Q901026","display_name":"Chromatin immunoprecipitation","level":5,"score":0.32109999656677246},{"id":"https://openalex.org/C64350747","wikidata":"https://www.wikidata.org/wiki/Q15334993","display_name":"RNA polymerase II","level":5,"score":0.29679998755455017},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.29429998993873596},{"id":"https://openalex.org/C27153228","wikidata":"https://www.wikidata.org/wiki/Q3787274","display_name":"Transcriptional regulation","level":4,"score":0.2851000130176544},{"id":"https://openalex.org/C67258246","wikidata":"https://www.wikidata.org/wiki/Q5065897","display_name":"ChIA-PET","level":5,"score":0.28040000796318054},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.2736000120639801},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.26980000734329224},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.2558000087738037}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.05.756609","doi":"https://doi.org/10.64898/2026.10.05.756609","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.05.756609","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.05.756609.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.05.756609","doi":"https://doi.org/10.64898/2026.10.05.756609","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.05.756609","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.05.756609.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.4043000042438507,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3265817843","display_name":null,"funder_award_id":"1556/22","funder_id":"https://openalex.org/F4320322252","funder_display_name":"Israel Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320322252","display_name":"Israel Science Foundation","ror":"https://ror.org/04sazxf24"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220719328.pdf","grobid_xml":"https://content.openalex.org/works/W7220719328.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W7117294876"],"related_works":["https://openalex.org/W2954785163","https://openalex.org/W3184347334","https://openalex.org/W3165269174","https://openalex.org/W640532418","https://openalex.org/W2025075195","https://openalex.org/W7117294876","https://openalex.org/W4378978752","https://openalex.org/W3046003662","https://openalex.org/W7131084485","https://openalex.org/W3042370703"],"abstract_inverted_index":{"Abstract":[0,200],"RNA-binding":[1],"proteins":[2],"(RBPs)":[3],"are":[4,24],"increasingly":[5],"recognized":[6],"to":[7,13,195],"perform":[8],"chromatin-associated":[9,41,81,185],"functions":[10],"in":[11,17,26,45,57,71,117,181,198],"addition":[12],"their":[14],"canonical":[15,65],"roles":[16,180],"RNA":[18,58,182],"regulation,":[19],"yet":[20],"how":[21],"these":[22],"activities":[23,42],"coordinated":[25],"human":[27],"embryonic":[28],"stem":[29],"cells":[30],"(hESCs)":[31],"remain":[32],"poorly":[33],"understood.":[34],"Here,":[35],"we":[36],"characterize":[37],"distinct":[38,80,179],"RNA-":[39],"and":[40,60,93,124,128,136,184,187],"for":[43,148,165],"RBM25":[44,51,84,103,123,145,166,173],"hESCs.":[46,118,199],"Transcriptome-wide":[47],"profiling":[48,76],"showed":[49,106],"that":[50,122,144,193],"preferentially":[52],"associates":[53],"with":[54,63,83,97,109,178],"transcripts":[55],"involved":[56,70],"processing":[59,183],"splicing,":[61],"consistent":[62],"its":[64],"role":[66],"as":[67,174],"an":[68],"RBP":[69],"post-transcriptional":[72],"regulation.":[73],"Genome-wide":[74],"chromatin":[75,167],"further":[77,120],"uncovered":[78],"a":[79,175,189],"program,":[82],"occupying":[85],"accessible":[86],"promoters":[87,153],"of":[88,154],"actively":[89],"expressed":[90,156],"cell-cycle":[91,157],"genes":[92],"introns":[94],"predominantly":[95],"associated":[96],"transcriptionally":[98],"inactive":[99],"neuronal":[100],"genes.":[101],"Interestingly,":[102],"chromatin-binding":[104,131],"sites":[105],"substantial":[107],"co-occupancy":[108],"regions":[110],"bound":[111],"by":[112],"the":[113],"transcription":[114],"factor":[115,177],"CEBPZ":[116,125,149,160],"We":[119],"show":[121],"physically":[126],"interact":[127],"exhibit":[129],"shared":[130],"patterns":[132],"at":[133,151],"both":[134],"promoter":[135],"intronic":[137],"regions.":[138],"Importantly,":[139],"reciprocal":[140],"depletion":[141],"experiments":[142],"revealed":[143],"is":[146,161],"required":[147,164],"occupancy":[150],"CCAAT-containing":[152],"highly":[155],"genes,":[158],"whereas":[159],"not":[162],"similarly":[163],"occupancy.":[168],"Together,":[169],"our":[170],"findings":[171],"establish":[172],"multifunctional":[176],"regulation":[186,197],"reveal":[188],"context-dependent":[190],"RBP\u2013TF":[191],"interaction":[192],"contributes":[194],"transcriptional":[196],"Figure":[201]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-07T00:00:00"}