{"id":"https://openalex.org/W7220683258","doi":"https://doi.org/10.64898/2026.10.03.756357","title":"Extracellular vesicle cross-linking coupled with solid-phase purification facilitates discovery of ribonucleoprotein complexes assembled on EV RNAs","display_name":"Extracellular vesicle cross-linking coupled with solid-phase purification facilitates discovery of ribonucleoprotein complexes assembled on EV RNAs","publication_year":2026,"publication_date":"2026-10-06","ids":{"openalex":"https://openalex.org/W7220683258","doi":"https://doi.org/10.64898/2026.10.03.756357","pmid":"https://pubmed.ncbi.nlm.nih.gov/42926424","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13692025"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.03.756357","doi":"https://doi.org/10.64898/2026.10.03.756357","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.03.756357","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.03.756357.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.03.756357.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5099431787","display_name":"Rajat Dhyani","orcid":"https://orcid.org/0009-0001-6777-7418","observed_orcids":["https://orcid.org/0009-0001-6777-7418"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rajat Dhyani","raw_affiliation_strings":["Department of Biochemistry, Vanderbilt University, Nashville, TN, USA"],"raw_orcid":"https://orcid.org/0009-0001-6777-7418","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Vanderbilt University, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101910813","display_name":"Stephen Clarke","orcid":"https://orcid.org/0000-0003-0830-1388","observed_orcids":["https://orcid.org/0000-0003-0830-1388"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen Clarke","raw_affiliation_strings":["Department of Biochemistry, Vanderbilt University, Nashville, TN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Vanderbilt University, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074859428","display_name":"Masashi Kuroda","orcid":"https://orcid.org/0000-0001-9226-0579","observed_orcids":["https://orcid.org/0000-0001-9226-0579"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Masashi Kuroda","raw_affiliation_strings":["Division of Diabetes and Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Diabetes and Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA","institution_ids":["https://openalex.org/I1285204247"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091410536","display_name":"Yohei Sanada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yohei Sanada","raw_affiliation_strings":["Division of Diabetes and Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Diabetes and Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA","institution_ids":["https://openalex.org/I1285204247"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157762423","display_name":"Marc Gorum","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I118073183","display_name":"Meharry Medical College","ror":"https://ror.org/00k63dq23","country_code":"US","type":"education","lineage":["https://openalex.org/I118073183"]},{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marc Gorum","raw_affiliation_strings":["Department of Biomedical Sciences, Meharry Medical College, Nashville, TN, USA","Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, Meharry Medical College, Nashville, TN, USA","institution_ids":["https://openalex.org/I118073183"]},{"raw_affiliation_string":"Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012943678","display_name":"Bahnisikha Barman","orcid":"https://orcid.org/0000-0002-7677-1595","observed_orcids":["https://orcid.org/0000-0002-7677-1595"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bahnisikha Barman","raw_affiliation_strings":["Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033934751","display_name":"Alissa M. Weaver","orcid":"https://orcid.org/0000-0002-4096-8636","observed_orcids":["https://orcid.org/0000-0002-4096-8636"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]},{"id":"https://openalex.org/I901861585","display_name":"Vanderbilt University Medical Center","ror":"https://ror.org/05dq2gs74","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210162197","https://openalex.org/I901861585"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alissa Weaver","raw_affiliation_strings":["Center for Extracellular Vesicle Research,Vanderbilt University School of Medicine, Nashville, TN, USA","Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA","Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"],"raw_orcid":"https://orcid.org/0000-0002-4096-8636","affiliations":[{"raw_affiliation_string":"Center for Extracellular Vesicle Research,Vanderbilt University School of Medicine, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446","https://openalex.org/I901861585"]},{"raw_affiliation_string":"Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446"]},{"raw_affiliation_string":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA","institution_ids":["https://openalex.org/I901861585"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024298382","display_name":"Takahisa Nakamura","orcid":"https://orcid.org/0000-0003-4922-4569","observed_orcids":["https://orcid.org/0000-0003-4922-4569","https://orcid.org/0009-0003-9954-8462"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Takahisa Nakamura","raw_affiliation_strings":["College of Medicine, University of Cincinnati, Cincinnati, OH, USA","Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA","Division of Developmental Biology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA","Division of Diabetes and Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Medicine, University of Cincinnati, Cincinnati, OH, USA","institution_ids":["https://openalex.org/I63135867"]},{"raw_affiliation_string":"Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA","institution_ids":["https://openalex.org/I63135867"]},{"raw_affiliation_string":"Division of Developmental Biology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA","institution_ids":["https://openalex.org/I1285204247"]},{"raw_affiliation_string":"Division of Diabetes and Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA","institution_ids":["https://openalex.org/I1285204247"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5017183292","display_name":"Manuel Ascano","orcid":"https://orcid.org/0000-0002-9370-5493","observed_orcids":["https://orcid.org/0000-0002-9370-5493"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]},{"id":"https://openalex.org/I901861585","display_name":"Vanderbilt University Medical Center","ror":"https://ror.org/05dq2gs74","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210162197","https://openalex.org/I901861585"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Manuel Ascano","raw_affiliation_strings":["Center for Extracellular Vesicle Research,Vanderbilt University School of Medicine, Nashville, TN, USA","Department of Biochemistry, Vanderbilt University, Nashville, TN, USA","Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA"],"raw_orcid":"https://orcid.org/0000-0002-9370-5493","affiliations":[{"raw_affiliation_string":"Center for Extracellular Vesicle Research,Vanderbilt University School of Medicine, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446","https://openalex.org/I901861585"]},{"raw_affiliation_string":"Department of Biochemistry, Vanderbilt University, Nashville, TN, USA","institution_ids":["https://openalex.org/I200719446"]},{"raw_affiliation_string":"Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA","institution_ids":["https://openalex.org/I901861585"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5017183292"],"corresponding_institution_ids":["https://openalex.org/I200719446","https://openalex.org/I901861585"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/extracellular-vesicles","display_name":"extracellular vesicles","score":1.0},{"id":"https://openalex.org/keywords/ribonucleoprotein-complexes","display_name":"ribonucleoprotein complexes","score":0.9760000109672546},{"id":"https://openalex.org/keywords/extracellular-rna","display_name":"extracellular RNA","score":0.8270000219345093},{"id":"https://openalex.org/keywords/protein-rna-interactions","display_name":"RNA-protein interactions","score":0.6129999756813049},{"id":"https://openalex.org/keywords/photo-crosslinking","display_name":"photocrosslinking","score":0.8309999704360962},{"id":"https://openalex.org/keywords/intercellular-rna-transfer","display_name":"intercellular RNA transfer","score":0.6579999923706055}],"concepts":[{"id":"https://openalex.org/C75934600","wikidata":"https://www.wikidata.org/wiki/Q419245","display_name":"Ribonucleoprotein","level":4,"score":0.8295999765396118},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6251999735832214},{"id":"https://openalex.org/C2908689518","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicle","level":5,"score":0.5864999890327454},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5600000023841858},{"id":"https://openalex.org/C2992929900","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicles","level":2,"score":0.5467000007629395},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5317000150680542},{"id":"https://openalex.org/C20518536","wikidata":"https://www.wikidata.org/wiki/Q6840469","display_name":"Microvesicles","level":4,"score":0.5087000131607056},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5062999725341797},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.45590001344680786},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.39750000834465027},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.39730000495910645},{"id":"https://openalex.org/C204389451","wikidata":"https://www.wikidata.org/wiki/Q178593","display_name":"Macromolecule","level":2,"score":0.3734000027179718},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.3702000081539154},{"id":"https://openalex.org/C41282012","wikidata":"https://www.wikidata.org/wiki/Q3928341","display_name":"RNA-binding protein","level":4,"score":0.3504999876022339},{"id":"https://openalex.org/C2781261824","wikidata":"https://www.wikidata.org/wiki/Q903634","display_name":"Exosome","level":5,"score":0.3476000130176544},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.34709998965263367},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3395000100135803},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.33889999985694885},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.33410000801086426},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.31929999589920044},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.31060001254081726},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.3070000112056732},{"id":"https://openalex.org/C203635412","wikidata":"https://www.wikidata.org/wiki/Q17007432","display_name":"Compartment (ship)","level":2,"score":0.29339998960494995},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28760001063346863},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.2858999967575073},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.2856999933719635},{"id":"https://openalex.org/C128943096","wikidata":"https://www.wikidata.org/wiki/Q17116953","display_name":"Messenger RNP","level":5,"score":0.2761000096797943},{"id":"https://openalex.org/C78885561","wikidata":"https://www.wikidata.org/wiki/Q189055","display_name":"Cellular compartment","level":3,"score":0.2687999904155731},{"id":"https://openalex.org/C94966510","wikidata":"https://www.wikidata.org/wiki/Q2252764","display_name":"DNA-binding protein","level":4,"score":0.2685999870300293},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.26190000772476196},{"id":"https://openalex.org/C152662350","wikidata":"https://www.wikidata.org/wiki/Q815297","display_name":"Systems biology","level":2,"score":0.26030001044273376},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.25850000977516174}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.10.03.756357","doi":"https://doi.org/10.64898/2026.10.03.756357","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.03.756357","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.03.756357.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:42926424","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42926424","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13692025","doi":null,"is_oa":true,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42926424/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.64898/2026.10.03.756357","doi":"https://doi.org/10.64898/2026.10.03.756357","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.03.756357","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/06/2026.10.03.756357.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1277615006","display_name":"Role of extracellular vesicles in the regulation of immunometabolism in obesity","funder_award_id":"R01DK123181","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G7325380665","display_name":"MFB: Characterization of the Biogenesis, Uptake, and Cellular Response to the Ribonucleoprotein Cargoes of Extracellular Vesicles using EV-CLASP","funder_award_id":"2330665","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G9000902591","display_name":"RNA silencing machinery in extracellular vesicle-mediated immunometabolic regulation","funder_award_id":"R01DK134646","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320309151","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7220683258.pdf","grobid_xml":"https://content.openalex.org/works/W7220683258.grobid-xml"},"referenced_works_count":46,"referenced_works":["https://openalex.org/W1518100516","https://openalex.org/W1601681275","https://openalex.org/W1982575478","https://openalex.org/W1986911150","https://openalex.org/W1992848590","https://openalex.org/W2032640007","https://openalex.org/W2039599418","https://openalex.org/W2041440237","https://openalex.org/W2041770101","https://openalex.org/W2048085228","https://openalex.org/W2050002679","https://openalex.org/W2052668460","https://openalex.org/W2053003053","https://openalex.org/W2053855164","https://openalex.org/W2053998703","https://openalex.org/W2055313665","https://openalex.org/W2094652474","https://openalex.org/W2096488278","https://openalex.org/W2106074866","https://openalex.org/W2115530456","https://openalex.org/W2117025491","https://openalex.org/W2140946052","https://openalex.org/W2167591654","https://openalex.org/W2168507741","https://openalex.org/W2307041907","https://openalex.org/W2537132940","https://openalex.org/W2567639438","https://openalex.org/W2607310450","https://openalex.org/W2610260244","https://openalex.org/W2767437846","https://openalex.org/W2899760200","https://openalex.org/W2904975846","https://openalex.org/W2907992023","https://openalex.org/W2943261504","https://openalex.org/W3005104290","https://openalex.org/W3028960763","https://openalex.org/W3033141682","https://openalex.org/W3039570519","https://openalex.org/W3105879279","https://openalex.org/W3157238284","https://openalex.org/W4249579699","https://openalex.org/W4319964795","https://openalex.org/W4327679993","https://openalex.org/W4391649568","https://openalex.org/W7117997526","https://openalex.org/W7161260951"],"related_works":["https://openalex.org/W3108765612","https://openalex.org/W7024839318","https://openalex.org/W7204939520","https://openalex.org/W2789725532","https://openalex.org/W4412841931","https://openalex.org/W4413929366","https://openalex.org/W3117667198","https://openalex.org/W7113457129","https://openalex.org/W2747205617","https://openalex.org/W3146939775"],"abstract_inverted_index":{"Extracellular":[0],"vesicles":[1],"(EVs)":[2],"are":[3,40,112,167],"fundamental":[4],"mediators":[5],"of":[6,48,51],"intercellular":[7],"communication":[8],"that":[9,91,202],"transfer":[10],"macromolecular":[11],"cargo,":[12],"including":[13],"proteins":[14,90],"and":[15,68,105,150,189,219,224],"RNAs,":[16],"to":[17,36,45,117,136,147,153,176,211],"functionally":[18],"reprogram":[19],"recipient":[20,33,148],"cells.":[21],"However,":[22],"the":[23,73,80,88,155,161,198],"molecular":[24],"processes":[25],"by":[26,86,180],"which":[27],"transferred":[28],"ribonucleoprotein":[29],"(RNP)":[30],"complexes":[31,166],"engage":[32],"host":[34,89],"machinery":[35],"influence":[37],"cellular":[38,98],"behavior":[39],"poorly":[41],"understood,":[42],"primarily":[43],"due":[44],"a":[46,192,208],"dearth":[47],"methods":[49],"capable":[50],"tracking":[52],"these":[53],"post-transfer":[54,84],"interactions.":[55],"To":[56],"address":[57],"this":[58],"gap,":[59],"we":[60],"developed":[61],"two":[62],"complementary":[63],"chemical":[64],"biology":[65],"platforms:":[66],"EV-CLASP":[67,71,188],"EV(r)-CLASP.":[69],"While":[70],"maps":[72],"native,":[74],"intra-vesicular":[75,138],"RNP":[76,139,165,200],"landscape":[77],"packaged":[78],"within":[79,160],"EV,":[81],"EV(r)-CLASP":[82,190],"captures":[83],"dynamics":[85],"identifying":[87],"directly":[92],"associate":[93],"with":[94,114],"EV-derived":[95],"RNAs":[96,122],"following":[97],"uptake.":[99],"Both":[100],"approaches":[101],"leverage":[102],"metabolic":[103],"labeling":[104],"photo-crosslinking":[106],"for":[107,184,196],"high-specificity":[108],"recovery.":[109],"Donor":[110],"cells":[111,149],"cultured":[113],"4-thiouridine":[115],"(4SU)":[116],"biosynthetically":[118],"label":[119],"newly":[120],"synthesized":[121],"during":[123],"EV":[124],"biogenesis.":[125],"Purified":[126],"EVs":[127,143],"can":[128,144],"be":[129,145],"crosslinked":[130,152],"using":[131,172],"365":[132],"nm":[133],"UV":[134],"light":[135],"capture":[137],"composition.":[140],"Alternatively,":[141],"4SU-labeled":[142],"delivered":[146],"subsequently":[151],"identify":[154],"primary":[156],"physical":[157],"interactions":[158],"formed":[159],"recipient-cell":[162],"environment.":[163],"Crosslinked":[164],"isolated":[168],"under":[169],"stringent":[170],"conditions":[171],"solid-phase":[173],"reversible":[174],"beads":[175],"minimize":[177],"background,":[178],"followed":[179],"quantitative":[181],"mass":[182],"spectrometry":[183],"protein":[185],"identification.":[186],"Together,":[187],"provide":[191],"robust,":[193],"versatile":[194],"framework":[195],"defining":[197],"dynamic":[199],"assemblies":[201],"facilitate":[203],"extracellular":[204],"RNA":[205],"communication,":[206],"offering":[207],"powerful":[209],"toolkit":[210],"uncover":[212],"novel":[213],"RNA-associated":[214],"regulatory":[215],"networks,":[216],"therapeutic":[217],"targets,":[218],"biomarkers":[220],"across":[221],"diverse":[222],"biological":[223],"disease":[225],"contexts.":[226]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-07T00:00:00"}