{"id":"https://openalex.org/W7219076599","doi":"https://doi.org/10.64898/2026.10.01.756105","title":"Positive control of the integrative and conjugative element Tn 916 and its limited gene expression in transconjugants","display_name":"Positive control of the integrative and conjugative element Tn 916 and its limited gene expression in transconjugants","publication_year":2026,"publication_date":"2026-10-02","ids":{"openalex":"https://openalex.org/W7219076599","doi":"https://doi.org/10.64898/2026.10.01.756105"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.01.756105","doi":"https://doi.org/10.64898/2026.10.01.756105","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.01.756105","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/02/2026.10.01.756105.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/02/2026.10.01.756105.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089604393","display_name":"Alexa F. S. Gomberg","orcid":"https://orcid.org/0000-0002-3596-9191","observed_orcids":["https://orcid.org/0000-0002-3596-9191"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexa F. S. Gomberg","raw_affiliation_strings":["Department of Biology Massachusetts Institute of Technology Cambridge, MA 02139"],"raw_orcid":"https://orcid.org/0000-0002-3596-9191","affiliations":[{"raw_affiliation_string":"Department of Biology Massachusetts Institute of Technology Cambridge, MA 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056293755","display_name":"Alan D. Grossman","orcid":"https://orcid.org/0000-0002-8235-7227","observed_orcids":["https://orcid.org/0000-0002-8235-7227"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Alan D. Grossman","raw_affiliation_strings":["Department of Biology Massachusetts Institute of Technology Cambridge, MA 02139"],"raw_orcid":"https://orcid.org/0000-0002-8235-7227","affiliations":[{"raw_affiliation_string":"Department of Biology Massachusetts Institute of Technology Cambridge, MA 02139","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5056293755"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tn916","display_name":"Tn916","score":0.8209999799728394},{"id":"https://openalex.org/keywords/integrative-and-conjugative-elements","display_name":"integrative and conjugative elements","score":0.996999979019165},{"id":"https://openalex.org/keywords/transconjugants","display_name":"transconjugants","score":0.8360000252723694},{"id":"https://openalex.org/keywords/transcriptional-regulation","display_name":"transcriptional regulation","score":0.6700000166893005},{"id":"https://openalex.org/keywords/host-cell-death","display_name":"host cell death","score":0.6629999876022339},{"id":"https://openalex.org/keywords/tetracycline-resistance","display_name":"tetracycline resistance","score":0.8889999985694885},{"id":"https://openalex.org/keywords/horizontal-gene-transfer","display_name":"horizontal gene transfer","score":0.7699999809265137},{"id":"https://openalex.org/keywords/bacillus-subtilis","display_name":"Bacillus subtilis","score":0.7990000247955322},{"id":"https://openalex.org/keywords/type-iv-secretion-system","display_name":"type IV secretion system","score":0.6600000262260437}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7520999908447266},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6615999937057495},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.6245999932289124},{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.5667999982833862},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.505299985408783},{"id":"https://openalex.org/C158448853","wikidata":"https://www.wikidata.org/wiki/Q425218","display_name":"Repressor","level":4,"score":0.49570000171661377},{"id":"https://openalex.org/C2909909245","wikidata":"https://www.wikidata.org/wiki/Q412550","display_name":"Integrases","level":4,"score":0.4489000141620636},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.42239999771118164},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.37450000643730164},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3693999946117401},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.366100013256073},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3637000024318695},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.3440000116825104},{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.33570000529289246},{"id":"https://openalex.org/C132376346","wikidata":"https://www.wikidata.org/wiki/Q7315962","display_name":"Response element","level":5,"score":0.3100999891757965},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.30709999799728394},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.30649998784065247},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.3057999908924103},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.30079999566078186},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.28940001130104065},{"id":"https://openalex.org/C53479859","wikidata":"https://www.wikidata.org/wiki/Q3064200","display_name":"Trans-acting","level":4,"score":0.2872999906539917},{"id":"https://openalex.org/C27153228","wikidata":"https://www.wikidata.org/wiki/Q3787274","display_name":"Transcriptional regulation","level":4,"score":0.2847999930381775},{"id":"https://openalex.org/C5527404","wikidata":"https://www.wikidata.org/wiki/Q2357310","display_name":"Structural gene","level":4,"score":0.2770000100135803},{"id":"https://openalex.org/C47319516","wikidata":"https://www.wikidata.org/wiki/Q306436","display_name":"Sigma factor","level":5,"score":0.2766999900341034},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.27489998936653137},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.27300000190734863}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.01.756105","doi":"https://doi.org/10.64898/2026.10.01.756105","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.01.756105","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/02/2026.10.01.756105.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.01.756105","doi":"https://doi.org/10.64898/2026.10.01.756105","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.01.756105","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/02/2026.10.01.756105.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000888522","display_name":"Mechanisms and regulation of replication, the cell cycle, gene expression, and horizontal gene transfer in prokaryotes, focusing on Bacillus subtilis.","funder_award_id":"R35GM148343","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7219076599.pdf","grobid_xml":"https://content.openalex.org/works/W7219076599.grobid-xml"},"referenced_works_count":76,"referenced_works":["https://openalex.org/W1493826396","https://openalex.org/W1506944866","https://openalex.org/W1554720878","https://openalex.org/W1560298788","https://openalex.org/W1832517154","https://openalex.org/W1834065866","https://openalex.org/W1951375663","https://openalex.org/W1960846065","https://openalex.org/W1971439989","https://openalex.org/W1978634693","https://openalex.org/W1986502527","https://openalex.org/W1986614154","https://openalex.org/W1998107070","https://openalex.org/W1998138820","https://openalex.org/W2000455772","https://openalex.org/W2022474924","https://openalex.org/W2023040179","https://openalex.org/W2036057054","https://openalex.org/W2036485896","https://openalex.org/W2036944766","https://openalex.org/W2037827003","https://openalex.org/W2048203847","https://openalex.org/W2048581247","https://openalex.org/W2051563423","https://openalex.org/W2061469117","https://openalex.org/W2064918078","https://openalex.org/W2068978083","https://openalex.org/W2069275673","https://openalex.org/W2080163848","https://openalex.org/W2088116028","https://openalex.org/W2088710570","https://openalex.org/W2094083595","https://openalex.org/W2099604042","https://openalex.org/W2106744027","https://openalex.org/W2108244474","https://openalex.org/W2123402293","https://openalex.org/W2123475271","https://openalex.org/W2123720278","https://openalex.org/W2124111522","https://openalex.org/W2126668940","https://openalex.org/W2127597865","https://openalex.org/W2130512803","https://openalex.org/W2134759932","https://openalex.org/W2142603486","https://openalex.org/W2150707538","https://openalex.org/W2155276251","https://openalex.org/W2160451071","https://openalex.org/W2244332155","https://openalex.org/W2316974384","https://openalex.org/W2462784838","https://openalex.org/W2491142891","https://openalex.org/W2512086450","https://openalex.org/W2528928751","https://openalex.org/W2586340362","https://openalex.org/W2734486695","https://openalex.org/W2791692859","https://openalex.org/W2791727059","https://openalex.org/W2944977335","https://openalex.org/W2953238103","https://openalex.org/W2954143028","https://openalex.org/W2966177293","https://openalex.org/W2973384993","https://openalex.org/W2979777308","https://openalex.org/W3045971147","https://openalex.org/W3157659281","https://openalex.org/W4242994810","https://openalex.org/W4307036852","https://openalex.org/W4307181113","https://openalex.org/W4320482632","https://openalex.org/W4324020343","https://openalex.org/W4386878256","https://openalex.org/W4403552969","https://openalex.org/W4405181763","https://openalex.org/W4411472741","https://openalex.org/W7098275465","https://openalex.org/W7128711983"],"related_works":["https://openalex.org/W4307181113","https://openalex.org/W4402322024","https://openalex.org/W2528928751","https://openalex.org/W6992022563","https://openalex.org/W1767836120","https://openalex.org/W165073713","https://openalex.org/W2519214528","https://openalex.org/W2124111522","https://openalex.org/W2052402112","https://openalex.org/W4280584411"],"abstract_inverted_index":{"Abstract":[0],"Integrative":[1],"and":[2,10,62,83,90,94,124,184,224,250,255],"conjugative":[3],"elements":[4],"(ICEs)":[5],"are":[6,225],"widespread":[7],"in":[8,58,67,100,165,174,192,227,262],"bacteria":[9],"typically":[11],"confer":[12],"important":[13],"phenotypes":[14],"to":[15,28,42,152,220,238],"their":[16],"hosts.":[17],"Following":[18],"excision":[19,128],"from":[20,117,137,162,171,189,247],"the":[21,33,130,182,257,263],"host":[22],"chromosome,":[23],"an":[24],"ICE":[25,51,214],"can":[26],"transfer":[27,237,254],"a":[29,44,49,105,148,239],"new":[30,240,264],"cell":[31,145],"through":[32],"element-encoded":[34],"conjugation":[35],"machinery":[36],"(a":[37],"type":[38],"IV":[39],"secretion":[40],"system)":[41],"create":[43],"transconjugant.":[45],"Tn":[46,74,101,198,231,245],"916":[47,75,102,199,232,246],",":[48,103],"well-studied":[50,211],"that":[52,88,114,217],"confers":[53],"tetracycline":[54],"resistance,":[55],"is":[56,125],"found":[57,87],"several":[59],"Gram-positive":[60],"pathogens":[61],"has":[63],"been":[64],"widely":[65],"studied":[66],"Bacillus":[68],"subtilis":[69],".":[70],"Unlike":[71],"most":[72,230],"ICEs,":[73],"causes":[76],"host-cell":[77],"death":[78,201],"following":[79,236],"its":[80,185],"activation":[81,261],"(excision":[82],"gene":[84,154,222],"expression).":[85],"We":[86],"orf7":[89,119],"orf8":[91],"(renamed":[92],"sig7":[93,122,139,164,173,191],"sig8":[95],"),":[96],"two":[97],"regulatory":[98],"genes":[99,233],"encode":[104],"two-subunit":[106],"(split)":[107],"sigma":[108],"factor":[109],"of":[110,129,134,169,180,197,202],"RNA":[111],"polymerase,":[112],"Sig7-8,":[113,135],"activates":[115],"transcription":[116,161,170,188],"P":[118,121,138,163,172,190],"(now":[120],")":[123,216],"required":[126],"for":[127,178,259],"element.":[131],"Importantly,":[132],"production":[133],"either":[136,195],"or":[140,159,200],"another":[141],"strong":[142],"promoter,":[143],"caused":[144,194],"death.":[146],"Using":[147],"Cre":[149],"recombinase":[150],"reporter":[151],"detect":[153],"expression,":[155],"we":[156],"detected":[157],"little":[158],"no":[160],"transconjugants.":[166,204],"This":[167,242],"lack":[168],"transconjugants":[175,193,249],"was":[176],"essential":[177],"fitness":[179],"both":[181],"element":[183],"host:":[186],"forcing":[187],"loss":[196],"nascent":[203,228],"Our":[205],"findings":[206],"indicate":[207],"that,":[208],"unlike":[209],"other":[210],"ICEs":[212],"(e.g.,":[213],"Bs1":[215],"use":[218],"repressors":[219],"control":[221],"expression":[223],"expressed":[226],"transconjugants,":[229],"remain":[234],"off":[235],"host.":[241,265],"regulation":[243],"prevents":[244],"killing":[248],"allows":[251],"successful":[252],"horizontal":[253],"maintains":[256],"potential":[258],"future":[260]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-10-04T00:00:00"}
