{"id":"https://openalex.org/W7215052002","doi":"https://doi.org/10.64898/2026.09.25.753432","title":"SEEKER: A genome-scale library-on-library screening platform for deciphering T cell recognition of antigen","display_name":"SEEKER: A genome-scale library-on-library screening platform for deciphering T cell recognition of antigen","publication_year":2026,"publication_date":"2026-10-01","ids":{"openalex":"https://openalex.org/W7215052002","doi":"https://doi.org/10.64898/2026.09.25.753432"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.25.753432","doi":"https://doi.org/10.64898/2026.09.25.753432","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.25.753432","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/01/2026.09.25.753432.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/01/2026.09.25.753432.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5154265968","display_name":"Sicheng Li","orcid":"https://orcid.org/0009-0004-1256-5209","observed_orcids":["https://orcid.org/0009-0004-1256-5209"]},"institutions":[{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Sicheng Li","raw_affiliation_strings":["Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China"],"raw_orcid":"https://orcid.org/0009-0004-1256-5209","affiliations":[{"raw_affiliation_string":"Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5154063707","display_name":"Li Lin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210099558","display_name":"Shanghai Changzheng Hospital","ror":"https://ror.org/0103dxn66","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210099558"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li Lin","raw_affiliation_strings":["Department of Rheumatology and Immunology, National Key Laboratory for Immunity and Inflammation, Changzheng Hospital, Shanghai, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Rheumatology and Immunology, National Key Laboratory for Immunity and Inflammation, Changzheng Hospital, Shanghai, China","institution_ids":["https://openalex.org/I4210099558"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008030140","display_name":"Wenxu Wu","orcid":"https://orcid.org/0009-0006-9277-1516","observed_orcids":["https://orcid.org/0009-0006-9277-1516"]},"institutions":[{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenxu Wu","raw_affiliation_strings":["Department of Automation, Tsinghua University, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Automation, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153695423","display_name":"Binwen Liu","orcid":"https://orcid.org/0009-0009-8175-814X","observed_orcids":["https://orcid.org/0009-0009-8175-814X"]},"institutions":[{"id":"https://openalex.org/I4405272711","display_name":"Changping Laboratory","ror":"https://ror.org/04kf84k17","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405272711"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Binwen Liu","raw_affiliation_strings":["Changping Laboratory, Beijing, China","Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China"],"raw_orcid":"https://orcid.org/0009-0009-8175-814X","affiliations":[{"raw_affiliation_string":"Changping Laboratory, Beijing, China","institution_ids":["https://openalex.org/I4405272711"]},{"raw_affiliation_string":"Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5154021136","display_name":"Huihui Ji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I17721919","display_name":"Shanxi Medical University","ror":"https://ror.org/0265d1010","country_code":"CN","type":"education","lineage":["https://openalex.org/I17721919"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huihui Ji","raw_affiliation_strings":["Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, Shanxi Medical University, Taiyuan, Shanxi Province, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, Shanxi Medical University, Taiyuan, Shanxi Province, China","institution_ids":["https://openalex.org/I17721919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153823596","display_name":"Zongyu Guo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405272711","display_name":"Changping Laboratory","ror":"https://ror.org/04kf84k17","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405272711"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zongyu Guo","raw_affiliation_strings":["Changping Laboratory, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Changping Laboratory, Beijing, China","institution_ids":["https://openalex.org/I4405272711"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018405566","display_name":"Yaqiong Liu","orcid":"https://orcid.org/0009-0007-2379-1297","observed_orcids":["https://orcid.org/0009-0007-2379-1297"]},"institutions":[{"id":"https://openalex.org/I4405272711","display_name":"Changping Laboratory","ror":"https://ror.org/04kf84k17","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405272711"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yaqiong Liu","raw_affiliation_strings":["Changping Laboratory, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Changping Laboratory, Beijing, China","institution_ids":["https://openalex.org/I4405272711"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115595941","display_name":"Jing Wu","orcid":"https://orcid.org/0009-0008-7787-7501","observed_orcids":["https://orcid.org/0009-0008-7787-7501","https://orcid.org/0009-0004-6080-5908"]},"institutions":[{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Juncheng Wu","raw_affiliation_strings":["Department of Automation, Tsinghua University, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Automation, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5154154462","display_name":"Bo Liu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405272711","display_name":"Changping Laboratory","ror":"https://ror.org/04kf84k17","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405272711"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Bo Liu","raw_affiliation_strings":["Changping Laboratory, Beijing, China","Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Changping Laboratory, Beijing, China","institution_ids":["https://openalex.org/I4405272711"]},{"raw_affiliation_string":"Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153576527","display_name":"Lisang Zhong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lisang Zhong","raw_affiliation_strings":["Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008093795","display_name":"Xiaowo Wang","orcid":"https://orcid.org/0000-0003-2965-8036","observed_orcids":["https://orcid.org/0000-0003-2965-8036"]},"institutions":[{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaowo Wang","raw_affiliation_strings":["Department of Automation, Tsinghua University, Beijing, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China"],"raw_orcid":"https://orcid.org/0000-0003-2965-8036","affiliations":[{"raw_affiliation_string":"Department of Automation, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5123757795","display_name":"Huji Xu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210099558","display_name":"Shanghai Changzheng Hospital","ror":"https://ror.org/0103dxn66","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210099558"]},{"id":"https://openalex.org/I4210160507","display_name":"Center for Life Sciences","ror":"https://ror.org/05kje8j93","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570","https://openalex.org/I4210160507","https://openalex.org/I99065089"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huji Xu","raw_affiliation_strings":["Department of Rheumatology and Immunology, National Key Laboratory for Immunity and Inflammation, Changzheng Hospital, Shanghai, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Rheumatology and Immunology, National Key Laboratory for Immunity and Inflammation, Changzheng Hospital, Shanghai, China","institution_ids":["https://openalex.org/I4210099558"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I4210160507","https://openalex.org/I99065089"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076526923","display_name":"Hai Qi","orcid":"https://orcid.org/0000-0001-5475-3989","observed_orcids":["https://orcid.org/0000-0001-5475-3989"]},"institutions":[{"id":"https://openalex.org/I17721919","display_name":"Shanxi Medical University","ror":"https://ror.org/0265d1010","country_code":"CN","type":"education","lineage":["https://openalex.org/I17721919"]},{"id":"https://openalex.org/I4210160507","display_name":"Center for Life Sciences","ror":"https://ror.org/05kje8j93","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570","https://openalex.org/I4210160507","https://openalex.org/I99065089"]},{"id":"https://openalex.org/I4405272711","display_name":"Changping Laboratory","ror":"https://ror.org/04kf84k17","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405272711"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Hai Qi","raw_affiliation_strings":["Changping Laboratory, Beijing, China","Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, Shanxi Medical University, Taiyuan, Shanxi Province, China","School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China"],"raw_orcid":"https://orcid.org/0000-0001-5475-3989","affiliations":[{"raw_affiliation_string":"Changping Laboratory, Beijing, China","institution_ids":["https://openalex.org/I4405272711"]},{"raw_affiliation_string":"Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, Shanxi Medical University, Taiyuan, Shanxi Province, China","institution_ids":["https://openalex.org/I17721919"]},{"raw_affiliation_string":"School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I99065089"]},{"raw_affiliation_string":"Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China","institution_ids":["https://openalex.org/I4210160507","https://openalex.org/I99065089"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5076526923"],"corresponding_institution_ids":["https://openalex.org/I17721919","https://openalex.org/I4210160507","https://openalex.org/I4405272711","https://openalex.org/I99065089"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.8560000061988831,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.8560000061988831,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12576","display_name":"vaccines and immunoinformatics approaches","score":0.09640000015497208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.021900000050663948,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/t-cell-receptor","display_name":"T cell receptor","score":0.7369999885559082},{"id":"https://openalex.org/keywords/clonal-expansion","display_name":"clonal expansion","score":0.8080000281333923},{"id":"https://openalex.org/keywords/ankylosing-spondylitis","display_name":"ankylosing spondylitis","score":0.8679999709129333},{"id":"https://openalex.org/keywords/hla-b27","display_name":"HLA-B27","score":0.9710000157356262},{"id":"https://openalex.org/keywords/autoantigens","display_name":"autoantigens","score":0.7070000171661377},{"id":"https://openalex.org/keywords/viral-epitopes","display_name":"viral epitopes","score":0.7390000224113464},{"id":"https://openalex.org/keywords/autoimmune-disease","display_name":"autoimmune disease","score":0.3790000081062317}],"concepts":[{"id":"https://openalex.org/C189938988","wikidata":"https://www.wikidata.org/wiki/Q24744925","display_name":"NFAT","level":4,"score":0.6970000267028809},{"id":"https://openalex.org/C19317047","wikidata":"https://www.wikidata.org/wiki/Q412037","display_name":"T-cell receptor","level":4,"score":0.6456999778747559},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5659999847412109},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5630000233650208},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.48339998722076416},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.4616999924182892},{"id":"https://openalex.org/C3875195","wikidata":"https://www.wikidata.org/wiki/Q5099363","display_name":"Chimeric antigen receptor","level":4,"score":0.45590001344680786},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.45419999957084656},{"id":"https://openalex.org/C188280979","wikidata":"https://www.wikidata.org/wiki/Q911125","display_name":"Human leukocyte antigen","level":3,"score":0.40560001134872437},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36559998989105225},{"id":"https://openalex.org/C2992881475","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T lymphocyte","level":3,"score":0.3619000017642975},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.34459999203681946},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34139999747276306},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.321399986743927},{"id":"https://openalex.org/C2777402515","wikidata":"https://www.wikidata.org/wiki/Q52849","display_name":"Ankylosing spondylitis","level":2,"score":0.3142000138759613},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.31360000371932983},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.30410000681877136},{"id":"https://openalex.org/C81089528","wikidata":"https://www.wikidata.org/wiki/Q5134986","display_name":"clone (Java method)","level":3,"score":0.29440000653266907},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.2827000021934509},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.26980000734329224},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.257999986410141}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.25.753432","doi":"https://doi.org/10.64898/2026.09.25.753432","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.25.753432","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/01/2026.09.25.753432.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.25.753432","doi":"https://doi.org/10.64898/2026.09.25.753432","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.25.753432","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/10/01/2026.09.25.753432.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4331311206","display_name":null,"funder_award_id":"31821003","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5966967","display_name":null,"funder_award_id":"JYB2025XDXM603","funder_id":"https://openalex.org/F4320321106","funder_display_name":"Ministry of Education of the People's Republic of China"},{"id":"https://openalex.org/G6557365388","display_name":null,"funder_award_id":"32130037","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G748315002","display_name":null,"funder_award_id":"2018AAA0100302","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G988892819","display_name":null,"funder_award_id":"82320108010","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321106","display_name":"Ministry of Education of the People's Republic of China","ror":"https://ror.org/01mv9t934"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7215052002.pdf","grobid_xml":"https://content.openalex.org/works/W7215052002.grobid-xml"},"referenced_works_count":56,"referenced_works":["https://openalex.org/W1829817483","https://openalex.org/W1841392767","https://openalex.org/W1971439989","https://openalex.org/W1979731533","https://openalex.org/W1989223689","https://openalex.org/W2007049021","https://openalex.org/W2010309262","https://openalex.org/W2024689720","https://openalex.org/W2038509173","https://openalex.org/W2049204549","https://openalex.org/W2086737677","https://openalex.org/W2095449486","https://openalex.org/W2105381977","https://openalex.org/W2124555673","https://openalex.org/W2149042084","https://openalex.org/W2151572058","https://openalex.org/W2157384708","https://openalex.org/W2160934060","https://openalex.org/W2507608410","https://openalex.org/W2778215749","https://openalex.org/W2789843538","https://openalex.org/W2884809460","https://openalex.org/W2889980427","https://openalex.org/W2899327929","https://openalex.org/W2900542760","https://openalex.org/W2901423492","https://openalex.org/W2911644242","https://openalex.org/W2912257772","https://openalex.org/W2922185282","https://openalex.org/W2951506174","https://openalex.org/W2953151710","https://openalex.org/W2956092422","https://openalex.org/W2968849363","https://openalex.org/W2977815242","https://openalex.org/W2982606708","https://openalex.org/W2984472267","https://openalex.org/W3017134886","https://openalex.org/W3024570138","https://openalex.org/W3040528450","https://openalex.org/W3093309977","https://openalex.org/W3125460427","https://openalex.org/W3131055609","https://openalex.org/W3164692211","https://openalex.org/W4211203343","https://openalex.org/W4225637095","https://openalex.org/W4225688524","https://openalex.org/W4309506674","https://openalex.org/W4310945495","https://openalex.org/W4311249164","https://openalex.org/W4319455163","https://openalex.org/W4366272177","https://openalex.org/W4389073220","https://openalex.org/W4406385630","https://openalex.org/W4407194952","https://openalex.org/W4407947514","https://openalex.org/W7172533782"],"related_works":["https://openalex.org/W7118170370","https://openalex.org/W4377011409","https://openalex.org/W4313368388","https://openalex.org/W2968849363","https://openalex.org/W7000897504","https://openalex.org/W4404064147","https://openalex.org/W3012355104","https://openalex.org/W1979731533","https://openalex.org/W3114418016","https://openalex.org/W4385281984"],"abstract_inverted_index":{"Abstract":[0],"Decoding":[1],"which":[2],"antigens":[3],"activate":[4],"a":[5,12,20,30,58,83],"given":[6],"T":[7,107,135],"cell":[8,33],"receptor":[9],"(TCR)":[10],"is":[11,67],"central":[13],"challenge":[14],"in":[15,57,145],"immunology.":[16],"We":[17],"present":[18],"SEEKER,":[19],"functional":[21],"genome-scale":[22],"library-on-library":[23],"screening":[24,91],"platform":[25],"built":[26],"on":[27],"three":[28],"elements:":[29],"Jurkat-derived":[31],"T-APC":[32],"(TAPCell)":[34],"that":[35,76],"combinatorially":[36],"expresses":[37],"one":[38,41],"TCR":[39],"and":[40,73,79,127,131],"peptide\u2013MHC":[42],"drawn":[43],"from":[44,109],"separate":[45],"libraries":[46,116],"together":[47],"with":[48],"an":[49],"NFAT":[50],"activation":[51],"reporter;":[52],"encapsulation":[53],"of":[54,125],"single":[55],"TAPCells":[56],"thermos-reversible":[59],"hydrogel":[60],"for":[61],"isolated":[62],"clonal":[63],"expansion,":[64],"so":[65],"recognition":[66],"read":[68],"out":[69],"as":[70],"intraclonal":[71],"activation;":[72],"dual-asymmetric":[74],"PCR":[75],"links":[77],"TCR-":[78],"pMHC-encoding":[80],"sequences":[81],"into":[82],"heritable":[84],"unit,":[85],"enabling":[86],"progressive":[87],"hit":[88],"enrichment":[89],"over":[90],"rounds.":[92],"SEEKER":[93],"interrogates":[94],"\u223c10":[95],"8":[96],"TCR-pMHC":[97],"combinations":[98],"per":[99],"run":[100],"without":[101],"prior":[102],"knowledge.":[103],"Screening":[104],"joint-infiltrating":[105],"CD8\u207a":[106],"cells":[108,136],"HLA-B*27\u207a":[110],"ankylosing":[111],"spondylitis":[112],"patients":[113],"against":[114],"proteome-wide":[115],"validated":[117],"53":[118],"TCR\u2013":[119],"peptide":[120],"pairs,":[121],"uncovered":[122],"extensive":[123],"cross-recognition":[124],"self":[126],"common":[128],"viral":[129],"epitopes,":[130],"revealed":[132],"highly":[133],"polyreactive":[134],"recognizing":[137],"up":[138],"to":[139],"80":[140],"autoantigens,":[141],"implicating":[142],"virus-triggered":[143],"poly-autoreactivity":[144],"multi-organ":[146],"autoimmune":[147],"diseases.":[148]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-10-02T00:00:00"}