{"id":"https://openalex.org/W7214803282","doi":"https://doi.org/10.64898/2026.09.29.755280","title":"Compartmentalized Nanozyme Transduction Enables Regenerative Molecular Amplification for Multiplexed miRNA-Based Prostate Cancer Stratification","display_name":"Compartmentalized Nanozyme Transduction Enables Regenerative Molecular Amplification for Multiplexed miRNA-Based Prostate Cancer Stratification","publication_year":2026,"publication_date":"2026-09-29","ids":{"openalex":"https://openalex.org/W7214803282","doi":"https://doi.org/10.64898/2026.09.29.755280"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.29.755280","doi":"https://doi.org/10.64898/2026.09.29.755280","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.29.755280","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/29/2026.09.29.755280.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/29/2026.09.29.755280.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002061008","display_name":"Ying Ma","orcid":"https://orcid.org/0000-0001-9282-8515","observed_orcids":["https://orcid.org/0000-0001-9282-8515","https://orcid.org/0009-0007-5367-6409"]},"institutions":[{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ying Ma","raw_affiliation_strings":["Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China","institution_ids":["https://openalex.org/I47720641"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060109156","display_name":"Xiang-Lan He","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiang-Lan He","raw_affiliation_strings":["Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China","institution_ids":["https://openalex.org/I47720641"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5145961531","display_name":"Jia-Qi Du","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jia-Qi Du","raw_affiliation_strings":["Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China","institution_ids":["https://openalex.org/I47720641"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066791028","display_name":"Meng-Meng Pan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140357","display_name":"Renmin Hospital of Wuhan University","ror":"https://ror.org/03ekhbz91","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210140357"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Meng-Meng Pan","raw_affiliation_strings":["Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China","institution_ids":["https://openalex.org/I4210140357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153432920","display_name":"Ming Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140357","display_name":"Renmin Hospital of Wuhan University","ror":"https://ror.org/03ekhbz91","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210140357"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ming Wang","raw_affiliation_strings":["Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China","institution_ids":["https://openalex.org/I4210140357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153411615","display_name":"Guan-Yu Qu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210160344","display_name":"Tongji Hospital","ror":"https://ror.org/04xy45965","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210160344"]},{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guan-Yu Qu","raw_affiliation_strings":["Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China","institution_ids":["https://openalex.org/I4210160344","https://openalex.org/I47720641"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018393782","display_name":"Ming Jiang","orcid":"https://orcid.org/0000-0002-1999-1866","observed_orcids":["https://orcid.org/0000-0002-1999-1866"]},"institutions":[{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ming Jiang","raw_affiliation_strings":["Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China","institution_ids":["https://openalex.org/I47720641"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153470176","display_name":"Li Xu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405260818","display_name":"Jinfeng Laboratory","ror":"https://ror.org/04amdcz96","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405260818"]},{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li Xu","raw_affiliation_strings":["Hubei Jiangxia Laboratory, Wuhan 430200, China","Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hubei Jiangxia Laboratory, Wuhan 430200, China","institution_ids":["https://openalex.org/I4405260818"]},{"raw_affiliation_string":"Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China","institution_ids":["https://openalex.org/I47720641"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5153455876","display_name":"Xu Yu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405260818","display_name":"Jinfeng Laboratory","ror":"https://ror.org/04amdcz96","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4405260818"]},{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xu Yu","raw_affiliation_strings":["Hubei Jiangxia Laboratory, Wuhan 430200, China","Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hubei Jiangxia Laboratory, Wuhan 430200, China","institution_ids":["https://openalex.org/I4405260818"]},{"raw_affiliation_string":"Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China","institution_ids":["https://openalex.org/I47720641"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5153455876"],"corresponding_institution_ids":["https://openalex.org/I4405260818","https://openalex.org/I47720641"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12302","display_name":"Advanced Nanomaterials in Catalysis","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nanozymes","display_name":"nanozymes","score":0.8889999985694885},{"id":"https://openalex.org/keywords/circulating-micrornas","display_name":"circulating microRNAs","score":0.7850000262260437},{"id":"https://openalex.org/keywords/prostate-cancer","display_name":"prostate cancer","score":0.8069999814033508},{"id":"https://openalex.org/keywords/liquid-biopsy","display_name":"liquid biopsy","score":0.9350000023841858},{"id":"https://openalex.org/keywords/duplex-specific-nuclease","display_name":"duplex-specific nuclease","score":0.8519999980926514},{"id":"https://openalex.org/keywords/signal-amplification","display_name":"signal amplification","score":0.746999979019165},{"id":"https://openalex.org/keywords/magnetic-separation","display_name":"magnetic separation","score":0.5049999952316284},{"id":"https://openalex.org/keywords/serum-biomarkers","display_name":"serum biomarkers","score":0.7799999713897705}],"concepts":[{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.6010000109672546},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.6007000207901001},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5957000255584717},{"id":"https://openalex.org/C2780192828","wikidata":"https://www.wikidata.org/wiki/Q181257","display_name":"Prostate cancer","level":3,"score":0.557200014591217},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4941999912261963},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.4830999970436096},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.4666000008583069},{"id":"https://openalex.org/C128972844","wikidata":"https://www.wikidata.org/wiki/Q418722","display_name":"Aptamer","level":2,"score":0.4560999870300293},{"id":"https://openalex.org/C19275194","wikidata":"https://www.wikidata.org/wiki/Q222903","display_name":"Multiplexing","level":2,"score":0.4408999979496002},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.4375},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.42419999837875366},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.39500001072883606},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.38769999146461487},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.38269999623298645},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31060001254081726},{"id":"https://openalex.org/C2777002142","wikidata":"https://www.wikidata.org/wiki/Q5031432","display_name":"Cancer biomarkers","level":3,"score":0.3102000057697296},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2985000014305115},{"id":"https://openalex.org/C3020000666","wikidata":"https://www.wikidata.org/wiki/Q17112037","display_name":"Bench to bedside","level":2,"score":0.2838999927043915},{"id":"https://openalex.org/C84211826","wikidata":"https://www.wikidata.org/wiki/Q5260078","display_name":"Deoxyribozyme","level":3,"score":0.28369998931884766},{"id":"https://openalex.org/C151872237","wikidata":"https://www.wikidata.org/wiki/Q1410759","display_name":"Homing (biology)","level":2,"score":0.2833000123500824},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.2606000006198883}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.29.755280","doi":"https://doi.org/10.64898/2026.09.29.755280","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.29.755280","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/29/2026.09.29.755280.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.29.755280","doi":"https://doi.org/10.64898/2026.09.29.755280","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.29.755280","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/29/2026.09.29.755280.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.9537000060081482,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3324582401","display_name":null,"funder_award_id":"22574059","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G4214499601","display_name":null,"funder_award_id":"22174049","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G4382154007","display_name":null,"funder_award_id":"2021CFB335","funder_id":"https://openalex.org/F4320322186","funder_display_name":"Natural Science Foundation of Hubei Province"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320322186","display_name":"Natural Science Foundation of Hubei Province","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214803282.pdf","grobid_xml":"https://content.openalex.org/works/W7214803282.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2517707868","https://openalex.org/W3167977408","https://openalex.org/W4404306158","https://openalex.org/W7171549749","https://openalex.org/W7204862792","https://openalex.org/W4280513539","https://openalex.org/W2530832271","https://openalex.org/W2484464157","https://openalex.org/W7154915819","https://openalex.org/W4415950847"],"abstract_inverted_index":{"Abstract":[0],"Circulating":[1],"microRNAs":[2],"(miRNAs)":[3],"have":[4],"emerged":[5],"as":[6],"promising":[7],"liquid":[8,177],"biopsy":[9],"markers;":[10],"however,":[11],"their":[12],"clinical":[13],"translation":[14],"is":[15],"restricted":[16],"by":[17],"low":[18],"abundance,":[19],"biological":[20],"heterogeneity,":[21],"and":[22,147,173],"the":[23],"complexity":[24],"of":[25,96,120,134,156],"multiplex":[26],"profiling.":[27],"Herein,":[28],"we":[29],"develop":[30],"a":[31,55,161],"t":[32],"argeted":[33],"c":[34],"ycling-mediated":[35],"d":[36],"uplex-":[37],"s":[38],"pecific":[39],"n":[40],"uclease":[41],"(":[42,50],"DSN":[43],")-":[44],"nano":[45],"zyme":[46],"signal":[47,79],"amplification":[48],"platform":[49,72,116],"TC-DSN-Nano":[51],")":[52],"that":[53,167],"establishes":[54,160],"compartmentalized":[56,163],"catalytic":[57,78,108,171],"transduction":[58,165],"strategy":[59],"for":[60,176],"multiplexed":[61,135],"miRNA":[62,89,104,136],"analysis.":[63],"By":[64],"encapsulating":[65],"gold":[66],"nanozymes":[67],"within":[68],"liposomal":[69],"nanoreactors,":[70],"this":[71],"spatially":[73],"separates":[74],"molecular":[75,169],"recognition":[76],"from":[77,99],"generation,":[80],"preserving":[81],"nanozyme":[82,164],"activity":[83],"during":[84],"sensor":[85],"construction.":[86],"Upon":[87],"target":[88],"recognition,":[90],"DSN-mediated":[91],"recycling":[92],"induces":[93],"regenerative":[94],"release":[95],"nanozyme-loaded":[97],"liposomes":[98],"magnetic":[100],"substrates,":[101],"converting":[102],"individual":[103],"molecules":[105],"into":[106],"amplified":[107],"outputs":[109],"without":[110],"conventional":[111],"nucleic":[112],"acid":[113],"amplification.":[114],"The":[115],"enables":[117,138],"programmable":[118],"profiling":[119],"six":[121],"prostate":[122],"cancer":[123,179],"(PCa)":[124],"associated":[125],"miRNAs":[126],"in":[127],"serum":[128],"samples.":[129],"Furthermore,":[130],"machine":[131],"learning-assisted":[132],"integration":[133],"signatures":[137],"accurate":[139],"discrimination":[140],"among":[141],"healthy":[142],"donors,":[143],"nonmetastatic":[144],"PCa":[145,149],"patients,":[146,150],"metastatic":[148],"achieving":[151],"an":[152],"overall":[153],"classification":[154],"accuracy":[155],"90.5%.":[157],"This":[158],"work":[159],"general":[162],"framework":[166],"integrates":[168],"recycling,":[170],"amplification,":[172],"computational":[174],"analysis":[175],"biopsy-based":[178],"stratification.":[180],"TOC":[181]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-30T00:00:00"}