{"id":"https://openalex.org/W7214767336","doi":"https://doi.org/10.64898/2026.09.27.754824","title":"Characterization of Stress Tolerance and Probiotic Traits of Streptococcus salivarius Strains Isolated from Wildflower Honey","display_name":"Characterization of Stress Tolerance and Probiotic Traits of Streptococcus salivarius Strains Isolated from Wildflower Honey","publication_year":2026,"publication_date":"2026-09-28","ids":{"openalex":"https://openalex.org/W7214767336","doi":"https://doi.org/10.64898/2026.09.27.754824"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.27.754824","doi":"https://doi.org/10.64898/2026.09.27.754824","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.27.754824","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.27.754824.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.27.754824.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5153527183","display_name":"Wonhui Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I113825674","display_name":"Handong Global University","ror":"https://ror.org/00txhkt32","country_code":"KR","type":"education","lineage":["https://openalex.org/I113825674"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Wonhui Lee","raw_affiliation_strings":["Department of Life Science, Handong Global University, Pohang, 37554, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Science, Handong Global University, Pohang, 37554, South Korea","institution_ids":["https://openalex.org/I113825674"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5153549978","display_name":"Yeon-Soo Oh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I113825674","display_name":"Handong Global University","ror":"https://ror.org/00txhkt32","country_code":"KR","type":"education","lineage":["https://openalex.org/I113825674"]},{"id":"https://openalex.org/I123900574","display_name":"Pohang University of Science and Technology","ror":"https://ror.org/04xysgw12","country_code":"KR","type":"education","lineage":["https://openalex.org/I123900574"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Yeon-Soo Oh","raw_affiliation_strings":["Department of Life Science, Handong Global University, Pohang, 37554, South Korea","Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Science, Handong Global University, Pohang, 37554, South Korea","institution_ids":["https://openalex.org/I113825674"]},{"raw_affiliation_string":"Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea","institution_ids":["https://openalex.org/I123900574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115606249","display_name":"Chan-Soo Ock","orcid":"https://orcid.org/0009-0003-8813-0474","observed_orcids":["https://orcid.org/0009-0003-8813-0474"]},"institutions":[{"id":"https://openalex.org/I123900574","display_name":"Pohang University of Science and Technology","ror":"https://ror.org/04xysgw12","country_code":"KR","type":"education","lineage":["https://openalex.org/I123900574"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Chan-Soo Ock","raw_affiliation_strings":["Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea","institution_ids":["https://openalex.org/I123900574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5135404592","display_name":"Ye-Eun Oh","orcid":"https://orcid.org/0009-0007-4168-660X","observed_orcids":["https://orcid.org/0009-0007-4168-660X"]},"institutions":[{"id":"https://openalex.org/I113825674","display_name":"Handong Global University","ror":"https://ror.org/00txhkt32","country_code":"KR","type":"education","lineage":["https://openalex.org/I113825674"]},{"id":"https://openalex.org/I123900574","display_name":"Pohang University of Science and Technology","ror":"https://ror.org/04xysgw12","country_code":"KR","type":"education","lineage":["https://openalex.org/I123900574"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Ye-Eun Oh","raw_affiliation_strings":["Department of Life Science, Handong Global University, Pohang, 37554, South Korea","Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Science, Handong Global University, Pohang, 37554, South Korea","institution_ids":["https://openalex.org/I113825674"]},{"raw_affiliation_string":"Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea","institution_ids":["https://openalex.org/I123900574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101764643","display_name":"Ha-Young Kim","orcid":"https://orcid.org/0000-0002-7613-5249","observed_orcids":["https://orcid.org/0000-0002-7613-5249"]},"institutions":[{"id":"https://openalex.org/I113825674","display_name":"Handong Global University","ror":"https://ror.org/00txhkt32","country_code":"KR","type":"education","lineage":["https://openalex.org/I113825674"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Ha-young Kim","raw_affiliation_strings":["Department of Life Science, Handong Global University, Pohang, 37554, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Science, Handong Global University, Pohang, 37554, South Korea","institution_ids":["https://openalex.org/I113825674"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102269080","display_name":"Hyung-Ki Do","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I113825674","display_name":"Handong Global University","ror":"https://ror.org/00txhkt32","country_code":"KR","type":"education","lineage":["https://openalex.org/I113825674"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Hyung-Ki Do","raw_affiliation_strings":["Department of Life Science, Handong Global University, Pohang, 37554, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Science, Handong Global University, Pohang, 37554, South Korea","institution_ids":["https://openalex.org/I113825674"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5153516700","display_name":"Chul-Won Hwang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I113825674","display_name":"Handong Global University","ror":"https://ror.org/00txhkt32","country_code":"KR","type":"education","lineage":["https://openalex.org/I113825674"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Chul-Won Hwang","raw_affiliation_strings":["Global Leadership School, Handong Global University, Pohang, 37554, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Global Leadership School, Handong Global University, Pohang, 37554, South Korea","institution_ids":["https://openalex.org/I113825674"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5102269080"],"corresponding_institution_ids":["https://openalex.org/I113825674"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11386","display_name":"Bee Products Chemical Analysis","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10257","display_name":"Oral microbiology and periodontitis research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/streptococcus-salivarius","display_name":"Streptococcus salivarius","score":1.0},{"id":"https://openalex.org/keywords/probiotic-potential","display_name":"probiotic potential","score":0.7590000033378601},{"id":"https://openalex.org/keywords/stress-tolerance","display_name":"stress tolerance","score":0.9929999709129333},{"id":"https://openalex.org/keywords/in-vitro-digestion","display_name":"in vitro digestion","score":0.5759999752044678},{"id":"https://openalex.org/keywords/phenol-tolerance","display_name":"phenol tolerance","score":0.5950000286102295},{"id":"https://openalex.org/keywords/hydrogen-peroxide-tolerance","display_name":"hydrogen peroxide resistance","score":0.9919999837875366},{"id":"https://openalex.org/keywords/cell-adhesion","display_name":"cell adhesion","score":0.6119999885559082},{"id":"https://openalex.org/keywords/mucin-adhesion","display_name":"mucin adhesion","score":0.7599999904632568},{"id":"https://openalex.org/keywords/cell-surface-hydrophobicity","display_name":"cell surface hydrophobicity","score":0.8960000276565552},{"id":"https://openalex.org/keywords/self-aggregation","display_name":"self-aggregation","score":0.9760000109672546},{"id":"https://openalex.org/keywords/antioxidant-activity","display_name":"antioxidant activity","score":0.7070000171661377},{"id":"https://openalex.org/keywords/extracellular-polymeric-substances","display_name":"extracellular polymeric substances","score":0.8199999928474426},{"id":"https://openalex.org/keywords/biofilm-formation","display_name":"biofilm formation","score":0.9789999723434448},{"id":"https://openalex.org/keywords/sucrose-metabolism","display_name":"sucrose metabolism","score":0.5199999809265137}],"concepts":[{"id":"https://openalex.org/C2780255968","wikidata":"https://www.wikidata.org/wiki/Q1816730","display_name":"Probiotic","level":3,"score":0.8762999773025513},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7802000045776367},{"id":"https://openalex.org/C2776244449","wikidata":"https://www.wikidata.org/wiki/Q139986","display_name":"Streptococcus salivarius","level":4,"score":0.760200023651123},{"id":"https://openalex.org/C2776833777","wikidata":"https://www.wikidata.org/wiki/Q3585615","display_name":"Wildflower","level":2,"score":0.6990000009536743},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6780999898910522},{"id":"https://openalex.org/C2778597767","wikidata":"https://www.wikidata.org/wiki/Q4027534","display_name":"Sucrose","level":2,"score":0.5324000120162964},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.5241000056266785},{"id":"https://openalex.org/C2780795924","wikidata":"https://www.wikidata.org/wiki/Q3825336","display_name":"Lactobacillus salivarius","level":4,"score":0.5160999894142151},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.4104999899864197},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4083000123500824},{"id":"https://openalex.org/C2778663903","wikidata":"https://www.wikidata.org/wiki/Q140018","display_name":"Streptococcaceae","level":3,"score":0.3968999981880188},{"id":"https://openalex.org/C179264091","wikidata":"https://www.wikidata.org/wiki/Q410862","display_name":"Mucin","level":2,"score":0.39259999990463257},{"id":"https://openalex.org/C58123911","wikidata":"https://www.wikidata.org/wiki/Q467410","display_name":"Biofilm","level":3,"score":0.38280001282691956},{"id":"https://openalex.org/C2777221637","wikidata":"https://www.wikidata.org/wiki/Q132644","display_name":"Lactobacillus acidophilus","level":4,"score":0.37720000743865967},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.3431999981403351},{"id":"https://openalex.org/C2778641530","wikidata":"https://www.wikidata.org/wiki/Q2902072","display_name":"Bifidobacterium bifidum","level":5,"score":0.32030001282691956},{"id":"https://openalex.org/C2780305828","wikidata":"https://www.wikidata.org/wiki/Q132656","display_name":"Bifidobacterium","level":4,"score":0.31130000948905945},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.29179999232292175},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.28949999809265137},{"id":"https://openalex.org/C192285135","wikidata":"https://www.wikidata.org/wiki/Q954757","display_name":"Colony-forming unit","level":3,"score":0.27889999747276306},{"id":"https://openalex.org/C2775980262","wikidata":"https://www.wikidata.org/wiki/Q218870","display_name":"Prebiotic","level":2,"score":0.26330000162124634},{"id":"https://openalex.org/C2778276568","wikidata":"https://www.wikidata.org/wiki/Q190161","display_name":"Streptococcus","level":3,"score":0.26330000162124634},{"id":"https://openalex.org/C14225744","wikidata":"https://www.wikidata.org/wiki/Q770530","display_name":"Antibiosis","level":3,"score":0.25850000977516174}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.27.754824","doi":"https://doi.org/10.64898/2026.09.27.754824","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.27.754824","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.27.754824.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.27.754824","doi":"https://doi.org/10.64898/2026.09.27.754824","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.27.754824","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.27.754824.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.5666999816894531}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214767336.pdf","grobid_xml":"https://content.openalex.org/works/W7214767336.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7204479679","https://openalex.org/W4407061999","https://openalex.org/W2774749384","https://openalex.org/W2783872538","https://openalex.org/W7221609752","https://openalex.org/W3109774918","https://openalex.org/W3135784528","https://openalex.org/W7140204351","https://openalex.org/W4389223128","https://openalex.org/W4380049336"],"abstract_inverted_index":{"Abstract":[0],"Honey":[1],"represents":[2],"a":[3,19,190,198],"distinctive":[4],"microbial":[5],"habitat":[6],"that":[7,156],"imposes":[8],"physicochemical":[9],"stresses":[10],"on":[11],"its":[12],"resident":[13],"microbiota":[14],"while":[15,195],"also":[16,110],"serving":[17],"as":[18,180,230],"potential":[20,61],"source":[21],"of":[22,157,171],"beneficial":[23],"microorganisms.":[24],"Two":[25],"Streptococcus":[26],"salivarius":[27,223],"strains,":[28],"DOW-5A":[29,85,127,196],"and":[30,42,52,76,124,132,142,166,215,225],"DOW-10,":[31],"isolated":[32],"from":[33,155],"Korean":[34],"wildflower":[35],"honey":[36],"were":[37,174],"identified":[38],"through":[39],"phenotypic":[40],"observation":[41],"16S":[43],"rRNA":[44],"sequencing.":[45],"Comprehensive":[46],"evaluation,":[47],"including":[48],"5":[49],"safety":[50],"assessments":[51],"14":[53],"functional":[54],"assays,":[55],"was":[56,178],"conducted":[57],"to":[58],"reveal":[59],"distinct":[60],"probiotic":[62,160,231],"characteristics":[63],"in":[64,189,201,212],"the":[65,91,104,122,158,181,219],"two":[66,220],"isolates.":[67],"Both":[68,108,145],"strains":[69,109,146,186],"exhibited":[70,111,128,147],"no":[71,78,152],"gelatinase":[72],"or":[73,118],"hemolytic":[74],"activity":[75],"showed":[77,96,137,197],"significant":[79,153],"cytotoxicity":[80],"toward":[81],"Caco-2":[82],"cells.":[83],"While":[84],"maintained":[86],"higher":[87,138],"viable":[88],"counts":[89],"during":[90,103],"simulated":[92,105],"gastric":[93],"phase,":[94],"DOW-10":[95,136],"an":[97,100],"increase":[98,200],"after":[99],"initial":[101],"decline":[102],"intestinal":[106],"phase.":[107],"growth":[112],"under":[113],"stress":[114,213],"conditions":[115],"involving":[116],"phenol":[117],"hydrogen":[119],"peroxide.":[120],"Among":[121],"cell-surface":[123,133],"adhesion-related":[125],"characteristics,":[126],"greater":[129],"mucin":[130],"adhesion":[131],"hydrophobicity,":[134],"whereas":[135],"autoaggregation":[139],"at":[140],"6":[141],"12":[143],"h.":[144],"DPPH":[148],"radical-scavenging":[149],"activity,":[150],"with":[151],"difference":[154],"reference":[159],"strain,":[161],"Lactobacillus":[162],"rhamnosus":[163],"GG.":[164],"Atypical":[165],"crystalline-like":[167],"colony":[168],"morphologies,":[169],"suggestive":[170],"EPS":[172],"production,":[173],"observed":[175],"when":[176],"sucrose":[177,205],"supplied":[179],"carbon":[182],"source.":[183],"Furthermore,":[184],"both":[185],"formed":[187],"biofilms":[188],"crystal":[191],"violet":[192],"staining":[193],"assay,":[194],"marked":[199],"biofilm":[202],"biomass":[203],"upon":[204],"supplementation.":[206],"These":[207],"findings":[208],"highlight":[209],"strain-specific":[210],"variation":[211],"tolerance":[214],"probiotic-associated":[216],"traits":[217],"between":[218],"honey-derived":[221],"Strep.":[222],"isolates":[224],"support":[226],"their":[227],"further":[228],"investigation":[229],"candidates.":[232]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-30T00:00:00"}