{"id":"https://openalex.org/W7214645956","doi":"https://doi.org/10.64898/2026.09.28.754875","title":"Fluorosphere-Assisted Nano-Biosensor for Detection of Circulating microRNAs in Non-Small Cell Lung Cancer","display_name":"Fluorosphere-Assisted Nano-Biosensor for Detection of Circulating microRNAs in Non-Small Cell Lung Cancer","publication_year":2026,"publication_date":"2026-09-28","ids":{"openalex":"https://openalex.org/W7214645956","doi":"https://doi.org/10.64898/2026.09.28.754875"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.28.754875","doi":"https://doi.org/10.64898/2026.09.28.754875","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.28.754875","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.28.754875.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.28.754875.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014412591","display_name":"Pradyumna Kumar Mishra","orcid":"https://orcid.org/0000-0002-0795-2819","observed_orcids":["https://orcid.org/0000-0002-0795-2819"]},"institutions":[{"id":"https://openalex.org/I37048141","display_name":"Indian Council of Medical Research","ror":"https://ror.org/0492wrx28","country_code":"IN","type":"government","lineage":["https://openalex.org/I37048141"]},{"id":"https://openalex.org/I4392021195","display_name":"ICMR National Institute for Research in Environmental Health","ror":"https://ror.org/008bp5f48","country_code":"IN","type":"education","lineage":["https://openalex.org/I4392021195"]},{"id":"https://openalex.org/I99364266","display_name":"Academy of Scientific and Innovative Research","ror":"https://ror.org/053rcsq61","country_code":"IN","type":"education","lineage":["https://openalex.org/I99364266"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Pradyumna Kumar Mishra","raw_affiliation_strings":["Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","Faculty of Medical Research, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India"],"raw_orcid":"https://orcid.org/0000-0002-0795-2819","affiliations":[{"raw_affiliation_string":"Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","institution_ids":["https://openalex.org/I37048141","https://openalex.org/I4392021195"]},{"raw_affiliation_string":"Faculty of Medical Research, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India","institution_ids":["https://openalex.org/I99364266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051487824","display_name":"Ruchita Shandilya","orcid":"https://orcid.org/0000-0002-2869-5233","observed_orcids":["https://orcid.org/0000-0002-2869-5233"]},"institutions":[{"id":"https://openalex.org/I37048141","display_name":"Indian Council of Medical Research","ror":"https://ror.org/0492wrx28","country_code":"IN","type":"government","lineage":["https://openalex.org/I37048141"]},{"id":"https://openalex.org/I4392021195","display_name":"ICMR National Institute for Research in Environmental Health","ror":"https://ror.org/008bp5f48","country_code":"IN","type":"education","lineage":["https://openalex.org/I4392021195"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Ruchita Shandilya","raw_affiliation_strings":["Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","institution_ids":["https://openalex.org/I37048141","https://openalex.org/I4392021195"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063421117","display_name":"Arpit Bhargava","orcid":"https://orcid.org/0000-0001-6450-6551","observed_orcids":["https://orcid.org/0000-0001-6450-6551"]},"institutions":[{"id":"https://openalex.org/I2801027606","display_name":"RKDF University","ror":"https://ror.org/01rfr0p20","country_code":"IN","type":"education","lineage":["https://openalex.org/I2801027606"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Arpit Bhargava","raw_affiliation_strings":["Faculty of Science, Ram Krishna Dharmarth Foundation (RKDF) University, Bhopal, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Science, Ram Krishna Dharmarth Foundation (RKDF) University, Bhopal, India","institution_ids":["https://openalex.org/I2801027606"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081916275","display_name":"Pooja Ratre","orcid":"https://orcid.org/0000-0003-3542-2943","observed_orcids":["https://orcid.org/0000-0003-3542-2943"]},"institutions":[{"id":"https://openalex.org/I37048141","display_name":"Indian Council of Medical Research","ror":"https://ror.org/0492wrx28","country_code":"IN","type":"government","lineage":["https://openalex.org/I37048141"]},{"id":"https://openalex.org/I4392021195","display_name":"ICMR National Institute for Research in Environmental Health","ror":"https://ror.org/008bp5f48","country_code":"IN","type":"education","lineage":["https://openalex.org/I4392021195"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Pooja Ratre","raw_affiliation_strings":["Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","institution_ids":["https://openalex.org/I37048141","https://openalex.org/I4392021195"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102723718","display_name":"Vikas Gurjar","orcid":"https://orcid.org/0009-0001-5348-0808","observed_orcids":["https://orcid.org/0009-0001-5348-0808"]},"institutions":[{"id":"https://openalex.org/I37048141","display_name":"Indian Council of Medical Research","ror":"https://ror.org/0492wrx28","country_code":"IN","type":"government","lineage":["https://openalex.org/I37048141"]},{"id":"https://openalex.org/I4392021195","display_name":"ICMR National Institute for Research in Environmental Health","ror":"https://ror.org/008bp5f48","country_code":"IN","type":"education","lineage":["https://openalex.org/I4392021195"]},{"id":"https://openalex.org/I99364266","display_name":"Academy of Scientific and Innovative Research","ror":"https://ror.org/053rcsq61","country_code":"IN","type":"education","lineage":["https://openalex.org/I99364266"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Vikas Gurjar","raw_affiliation_strings":["Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","Faculty of Medical Research, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","institution_ids":["https://openalex.org/I37048141","https://openalex.org/I4392021195"]},{"raw_affiliation_string":"Faculty of Medical Research, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India","institution_ids":["https://openalex.org/I99364266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115868950","display_name":"Olga Goryacheva","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I125219893","display_name":"Saratov State University","ror":"https://ror.org/05jcsqx24","country_code":"RU","type":"education","lineage":["https://openalex.org/I125219893"]},{"id":"https://openalex.org/I4388482657","display_name":"Shenzhen MSU-BIT University","ror":"https://ror.org/02q963474","country_code":"CN","type":"education","lineage":["https://openalex.org/I4388482657"]}],"countries":["CN","RU"],"is_corresponding":false,"raw_author_name":"Olga Goryacheva","raw_affiliation_strings":["Faculty of Materials Science, Shenzhen MSU-BIT University, Shenzhen 518172, People\u2019s Republic of China","Institute of Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Materials Science, Shenzhen MSU-BIT University, Shenzhen 518172, People\u2019s Republic of China","institution_ids":["https://openalex.org/I4388482657"]},{"raw_affiliation_string":"Institute of Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia","institution_ids":["https://openalex.org/I125219893"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5140097417","display_name":"Rajnarayan Tiwari","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I37048141","display_name":"Indian Council of Medical Research","ror":"https://ror.org/0492wrx28","country_code":"IN","type":"government","lineage":["https://openalex.org/I37048141"]},{"id":"https://openalex.org/I4392021195","display_name":"ICMR National Institute for Research in Environmental Health","ror":"https://ror.org/008bp5f48","country_code":"IN","type":"education","lineage":["https://openalex.org/I4392021195"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rajnarayan Tiwari","raw_affiliation_strings":["Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Environmental Biotechnology, Genetics & Molecular Biology (EBGMB), ICMR-National Institute for Research in Environmental Health (NIREH), Bhopal, India","institution_ids":["https://openalex.org/I37048141","https://openalex.org/I4392021195"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025016989","display_name":"Irina Yu. Goryacheva","orcid":"https://orcid.org/0000-0003-1781-6180","observed_orcids":["https://orcid.org/0000-0003-1781-6180"]},"institutions":[{"id":"https://openalex.org/I125219893","display_name":"Saratov State University","ror":"https://ror.org/05jcsqx24","country_code":"RU","type":"education","lineage":["https://openalex.org/I125219893"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Irina Yu Goryacheva","raw_affiliation_strings":["Institute of Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia","institution_ids":["https://openalex.org/I125219893"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5014412591"],"corresponding_institution_ids":["https://openalex.org/I37048141","https://openalex.org/I4392021195","https://openalex.org/I99364266"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.8144999742507935,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.8144999742507935,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.13989999890327454,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.008299999870359898,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/non-small-cell-lung-cancer","display_name":"non-small cell lung cancer","score":0.9449999928474426},{"id":"https://openalex.org/keywords/mir-16-5p","display_name":"miR-16-5p","score":0.7900000214576721},{"id":"https://openalex.org/keywords/locked-nucleic-acid-probes","display_name":"locked nucleic acid probes","score":0.9100000262260437},{"id":"https://openalex.org/keywords/noninvasive-biomarkers","display_name":"non-invasive biomarkers","score":0.7149999737739563},{"id":"https://openalex.org/keywords/cancer-diagnosis","display_name":"cancer diagnosis","score":0.5350000262260437}],"concepts":[{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.8003000020980835},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5138999819755554},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.43720000982284546},{"id":"https://openalex.org/C61238886","wikidata":"https://www.wikidata.org/wiki/Q5121689","display_name":"Circulating tumor cell","level":4,"score":0.41609999537467957},{"id":"https://openalex.org/C2776256026","wikidata":"https://www.wikidata.org/wiki/Q47912","display_name":"Lung cancer","level":2,"score":0.41600000858306885},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.41370001435279846},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.39879998564720154},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.39809998869895935},{"id":"https://openalex.org/C14363569","wikidata":"https://www.wikidata.org/wiki/Q418639","display_name":"Locked nucleic acid","level":4,"score":0.3928999900817871},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.3815000057220459},{"id":"https://openalex.org/C205711294","wikidata":"https://www.wikidata.org/wiki/Q176953","display_name":"Rendering (computer graphics)","level":2,"score":0.3799999952316284},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.3409000039100647},{"id":"https://openalex.org/C119203544","wikidata":"https://www.wikidata.org/wiki/Q425891","display_name":"Molecular beacon","level":4,"score":0.33799999952316284},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.33629998564720154},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.33340001106262207},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33059999346733093},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31630000472068787},{"id":"https://openalex.org/C2983449737","wikidata":"https://www.wikidata.org/wiki/Q177719","display_name":"Clinical diagnosis","level":2,"score":0.3009999990463257},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.28679999709129333},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.2556000053882599},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.25130000710487366}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.28.754875","doi":"https://doi.org/10.64898/2026.09.28.754875","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.28.754875","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.28.754875.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.28.754875","doi":"https://doi.org/10.64898/2026.09.28.754875","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.28.754875","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.28.754875.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320720","display_name":"Indian Council of Medical Research","ror":"https://ror.org/0492wrx28"},{"id":"https://openalex.org/F4320324333","display_name":"Ministry of Health and Family Welfare","ror":"https://ror.org/04pqetg36"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214645956.pdf","grobid_xml":"https://content.openalex.org/works/W7214645956.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W2888992486","https://openalex.org/W2891887198","https://openalex.org/W2904169549","https://openalex.org/W2904490758","https://openalex.org/W2912124375","https://openalex.org/W2968150703","https://openalex.org/W2981903137","https://openalex.org/W3006340657","https://openalex.org/W3044627739","https://openalex.org/W3048656447","https://openalex.org/W3128646645","https://openalex.org/W3133601828","https://openalex.org/W4387735603","https://openalex.org/W4394687063","https://openalex.org/W4402886999","https://openalex.org/W4404398436"],"related_works":["https://openalex.org/W7128544571","https://openalex.org/W7213596487","https://openalex.org/W4200173033","https://openalex.org/W3010732140","https://openalex.org/W7048572407","https://openalex.org/W2160448685","https://openalex.org/W4381252382","https://openalex.org/W4226300539","https://openalex.org/W4409038611","https://openalex.org/W2743956930"],"abstract_inverted_index":{"Abstract":[0],"Non-small-cell":[1],"lung":[2],"cancer":[3],"(NSCLC)":[4],"diagnosis":[5,270],"is":[6],"challenged":[7],"owing":[8],"to":[9,44,182,211,274],"the":[10,83,93,119,124,128,133,164,203,216],"need":[11],"for":[12,61,73,106,208,222,251,265],"reliable,":[13],"non-invasive":[14],"biomarkers.":[15],"Circulating":[16],"cell-free":[17],"microRNAs":[18],"(ccf-miRNAs)":[19],"are":[20,180,234],"promising":[21],"candidates,":[22],"but":[23],"their":[24,74,171],"low":[25,172],"levels":[26],"and":[27,47,65,88,95,108,113,127,142,152,159,174,194,231,248,260,271],"short":[28,175],"sequences":[29],"in":[30,189,225,238,255,268],"blood":[31],"complicate":[32],"detection.":[33,75,98],"In":[34],"this":[35,185],"study,":[36],"a":[37,79,246],"novel":[38],"nano-hybrid":[39,242],"fluorescent":[40,58,243],"array":[41,54,244],"was":[42,101],"developed":[43],"enable":[45],"rapid":[46,247],"sensitive":[48],"identification":[49],"of":[50,56,97,140,167,196,202],"NSCLC-related":[51],"ccf-miRNAs.":[52],"The":[53,99,115,135,199],"consists":[55],"poly(T)-modified":[57,120],"nano-polystyrene":[59,157],"beads":[60,121,158],"capturing":[62],"target":[63,84,125],"miRNAs,":[64,126],"fluorophore-labeled,":[66],"sequence-specific":[67,160],"locked":[68],"nucleic":[69],"acid":[70],"(LNA)":[71],"probes":[72,130,162],"This":[76,241],"design":[77,262],"forms":[78],"sandwich":[80],"structure":[81],"with":[82,156],"miRNA.":[85],"Nano":[86],"cytometry":[87],"fluorescence":[89],"microscopy":[90],"assessed":[91],"both":[92],"capture":[94,205],"specificity":[96],"assay":[100,136],"tested":[102],"on":[103],"plasma":[104,145],"samples":[105],"miR-16-5p":[107,141],"U6,":[109],"evaluating":[110],"selectivity,":[111,150],"sensitivity,":[112,151],"reproducibility.":[114,153],"results":[116],"showed":[117],"that":[118],"efficiently":[122],"captured":[123],"LNA":[129,161,217],"accurately":[131],"distinguished":[132],"sequences.":[134],"enabled":[137],"direct":[138],"detection":[139,224],"U6":[143],"from":[144],"without":[146],"amplification,":[147],"demonstrating":[148],"high":[149],"Combining":[154],"enrichment":[155],"addressed":[163],"main":[165],"challenges":[166],"ccf-miRNA":[168],"detection,":[169],"namely":[170],"abundance":[173],"sequence":[176],"length.":[177],"As":[178],"ccf-miRNAs":[179,254],"linked":[181],"NSCLC":[183,269],"progression,":[184],"method":[186],"could":[187],"assist":[188],"early":[190],"diagnosis,":[191],"disease":[192],"monitoring,":[193,272],"assessment":[195],"treatment":[197],"response.":[198],"target-independent":[200],"nature":[201],"poly(T)":[204],"layer":[206],"allows":[207],"easy":[209],"calibration":[210],"new":[212],"miRNAs":[213],"by":[214],"changing":[215],"probes,":[218],"rendering":[219],"it":[220],"suitable":[221],"multiplex":[223],"clinical":[226,232],"settings.":[227],"However,":[228],"further":[229],"preclinical":[230],"studies":[233],"needed":[235],"before":[236],"adoption":[237],"routine":[239],"practice.":[240],"presents":[245],"reliable":[249],"approach":[250],"detecting":[252],"low-abundance":[253],"plasma.":[256],"It\u2019s":[257],"encouraging":[258],"performance":[259],"flexible":[261],"show":[263],"potential":[264],"future":[266],"application":[267],"subject":[273],"additional":[275],"confirmation.":[276]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-29T00:00:00"}