{"id":"https://openalex.org/W7214638904","doi":"https://doi.org/10.64898/2026.09.24.753995","title":"Reassessing the contribution of the histone H3 tail to KRAB\u2013DNMT3L-mediated epigenetic silencing","display_name":"Reassessing the contribution of the histone H3 tail to KRAB\u2013DNMT3L-mediated epigenetic silencing","publication_year":2026,"publication_date":"2026-09-28","ids":{"openalex":"https://openalex.org/W7214638904","doi":"https://doi.org/10.64898/2026.09.24.753995"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.24.753995","doi":"https://doi.org/10.64898/2026.09.24.753995","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.24.753995","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.24.753995.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.24.753995.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101671994","display_name":"Minglei Song","orcid":"https://orcid.org/0009-0004-3116-6393","observed_orcids":["https://orcid.org/0009-0004-3116-6393"]},"institutions":[{"id":"https://openalex.org/I3045169105","display_name":"Southern University of Science and Technology","ror":"https://ror.org/049tv2d57","country_code":"CN","type":"education","lineage":["https://openalex.org/I3045169105"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Minglei Song","raw_affiliation_strings":["Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen, China","institution_ids":["https://openalex.org/I3045169105"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038793343","display_name":"Jiajun Lei","orcid":"https://orcid.org/0000-0001-5168-7541","observed_orcids":["https://orcid.org/0000-0001-5168-7541"]},"institutions":[{"id":"https://openalex.org/I3045169105","display_name":"Southern University of Science and Technology","ror":"https://ror.org/049tv2d57","country_code":"CN","type":"education","lineage":["https://openalex.org/I3045169105"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiajun Lei","raw_affiliation_strings":["Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen, China","institution_ids":["https://openalex.org/I3045169105"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5153349291","display_name":"Jian-Kang Zhu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111950717","display_name":"Macau University of Science and Technology","ror":"https://ror.org/03jqs2n27","country_code":"MO","type":"education","lineage":["https://openalex.org/I111950717","https://openalex.org/I4391767947"]},{"id":"https://openalex.org/I3045169105","display_name":"Southern University of Science and Technology","ror":"https://ror.org/049tv2d57","country_code":"CN","type":"education","lineage":["https://openalex.org/I3045169105"]}],"countries":["CN","MO"],"is_corresponding":true,"raw_author_name":"Jian-Kang Zhu","raw_affiliation_strings":["Faculty of Medicine, Macau University of Science and Technology, Macau SAR, China","Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Medicine, Macau University of Science and Technology, Macau SAR, China","institution_ids":["https://openalex.org/I111950717"]},{"raw_affiliation_string":"Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen, China","institution_ids":["https://openalex.org/I3045169105"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5153349291"],"corresponding_institution_ids":["https://openalex.org/I111950717","https://openalex.org/I3045169105"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8817999958992004,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8817999958992004,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.09470000118017197,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.005499999970197678,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/histone-h3-tail","display_name":"histone H3 tail","score":0.8659999966621399},{"id":"https://openalex.org/keywords/epigenetic-silencing","display_name":"epigenetic silencing","score":0.8880000114440918},{"id":"https://openalex.org/keywords/h3k4","display_name":"H3K4","score":0.6610000133514404},{"id":"https://openalex.org/keywords/dnmt3a","display_name":"DNMT3A","score":0.6800000071525574},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.6380000114440918},{"id":"https://openalex.org/keywords/hek293t-cells","display_name":"HEK293T cells","score":0.824999988079071}],"concepts":[{"id":"https://openalex.org/C2776745794","wikidata":"https://www.wikidata.org/wiki/Q24777451","display_name":"Histone H3","level":4,"score":0.7556999921798706},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5978999733924866},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.588699996471405},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.5382000207901001},{"id":"https://openalex.org/C186310378","wikidata":"https://www.wikidata.org/wiki/Q941796","display_name":"Psychological repression","level":4,"score":0.5356000065803528},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5220000147819519},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.510699987411499},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.4740000069141388},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.4684000015258789},{"id":"https://openalex.org/C2779856020","wikidata":"https://www.wikidata.org/wiki/Q218642","display_name":"Alanine","level":3,"score":0.3675000071525574},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.31779998540878296},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.2822999954223633},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2809000015258789},{"id":"https://openalex.org/C117717151","wikidata":"https://www.wikidata.org/wiki/Q3589155","display_name":"Epigenome","level":5,"score":0.274399995803833},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2741999924182892},{"id":"https://openalex.org/C2778305200","wikidata":"https://www.wikidata.org/wiki/Q174378","display_name":"Histone deacetylase","level":4,"score":0.26499998569488525},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.25929999351501465},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.25040000677108765}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.24.753995","doi":"https://doi.org/10.64898/2026.09.24.753995","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.24.753995","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.24.753995.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.24.753995","doi":"https://doi.org/10.64898/2026.09.24.753995","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.24.753995","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/28/2026.09.24.753995.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214638904.pdf","grobid_xml":"https://content.openalex.org/works/W7214638904.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4210909482","https://openalex.org/W2013928795","https://openalex.org/W7154210827","https://openalex.org/W2943200601","https://openalex.org/W7071668287","https://openalex.org/W1981354816","https://openalex.org/W2084099620","https://openalex.org/W2947427776","https://openalex.org/W2132330275","https://openalex.org/W2126606466"],"abstract_inverted_index":{"Abstract":[0],"Neumann":[1],"et":[2],"al.":[3],"introduced":[4],"CHARM,":[5],"a":[6,12,32,57,138],"compact":[7],"epigenetic":[8],"silencer":[9],"in":[10,46,79,119],"which":[11],"histone":[13],"H3":[14,44,62,133],"tail":[15,45,63,134],"fused":[16,33],"to":[17,21,68,77,94,117],"DNMT3L":[18],"was":[19],"proposed":[20],"recruit":[22],"and":[23,49,71,111],"stimulate":[24],"endogenous":[25,109],"DNMT3A,":[26],"enabling":[27],"durable":[28],"gene":[29],"repression":[30],"without":[31],"DNMT3A":[34],"catalytic":[35],"domain.":[36],"Here,":[37],"we":[38],"evaluated":[39],"the":[40,43,61,73,103,113,131,143,147],"contribution":[41],"of":[42,91],"independent":[47],"reporter":[48,55],"endogenous-gene":[50],"contexts.":[51],"In":[52],"an":[53],"SNRPN":[54],"system,":[56],"KRAB\u2013DNMT3L\u2013dCas9":[58,144],"construct":[59,106],"lacking":[60],"displayed":[64],"silencing":[65],"kinetics":[66],"comparable":[67],"CRISPRcharm":[69,80,97,120],"Kv2,":[70],"mutating":[72,112],"critical":[74,114],"H3K4":[75,115],"residue":[76,116],"alanine":[78,118],"Kv2":[81,98,121],"did":[82,99,122],"not":[83,100,123,136],"compromise":[84,124],"this":[85,125],"silencing.":[86],"Similarly,":[87],"after":[88],"transient":[89],"delivery":[90],"editor":[92],"mRNAs":[93],"HEK293T":[95],"cells,":[96],"consistently":[101],"outperform":[102],"corresponding":[104],"H3-tail-free":[105],"at":[107],"three":[108],"loci,":[110],"activity.":[126],"These":[127],"observations":[128],"suggest":[129],"that":[130],"engineered":[132],"does":[135],"confer":[137],"general":[139],"functional":[140],"advantage":[141],"within":[142],"architecture":[145],"under":[146],"conditions":[148],"tested.":[149]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-29T00:00:00"}