{"id":"https://openalex.org/W7214514919","doi":null,"title":"Optimization of batch in vitro transcription reaction with co-transcriptional capping of mRNA","display_name":"Optimization of batch in vitro transcription reaction with co-transcriptional capping of mRNA","publication_year":2026,"publication_date":"2026-09-23","ids":{"openalex":"https://openalex.org/W7214514919"},"language":"sl","primary_location":{"id":"pmh:oai:repozitorij.uni-lj.si:IzpisGradiva.php?id=188475","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/20.500.12556/RUL-188475","pdf_url":null,"source":{"id":"https://openalex.org/S4377196268","display_name":"Repository of the University of Ljubljana (University of Ljubljana)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I153976015","host_organization_name":"University of Ljubljana","host_organization_lineage":["https://openalex.org/I153976015"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5152989326","display_name":"Uma Jordan Ferbe\u017ear","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jordan Ferbe\u017ear, Uma","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5152989326"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.7240999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.7240999937057495,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.12020000070333481,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.09860000014305115,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/in-vitro-transcription","display_name":"in vitro transcription","score":0.972000002861023},{"id":"https://openalex.org/keywords/mrna-capping","display_name":"mRNA capping","score":0.7580000162124634},{"id":"https://openalex.org/keywords/experimental-design","display_name":"experimental design","score":0.753000020980835},{"id":"https://openalex.org/keywords/double-stranded-rna","display_name":"double-stranded RNA","score":0.6499999761581421},{"id":"https://openalex.org/keywords/immunogenicity","display_name":"immunogenicity","score":0.6269999742507935},{"id":"https://openalex.org/keywords/enhanced-green-fluorescent-protein","display_name":"enhanced green fluorescent protein","score":0.4860000014305115},{"id":"https://openalex.org/keywords/cost-efficiency","display_name":"cost efficiency","score":0.5},{"id":"https://openalex.org/keywords/mrna-therapeutics","display_name":"mRNA therapeutics","score":0.4880000054836273},{"id":"https://openalex.org/keywords/t7-rna-polymerase","display_name":"T7 RNA polymerase","score":0.5350000262260437},{"id":"https://openalex.org/keywords/reaction-temperature","display_name":"reaction temperature","score":0.3409999907016754},{"id":"https://openalex.org/keywords/incubation-time","display_name":"incubation time","score":0.4729999899864197}],"concepts":[{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.7994999885559082},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7390000224113464},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5491999983787537},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5440999865531921},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5098999738693237},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.37369999289512634},{"id":"https://openalex.org/C2191507","wikidata":"https://www.wikidata.org/wiki/Q7277185","display_name":"RNA silencing","level":5,"score":0.3596000075340271},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3562999963760376},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.29339998960494995},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.2865999937057495},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2815000116825104},{"id":"https://openalex.org/C70219777","wikidata":"https://www.wikidata.org/wiki/Q14911527","display_name":"Nonsense-mediated decay","level":5,"score":0.2782999873161316},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2759000062942505},{"id":"https://openalex.org/C194857396","wikidata":"https://www.wikidata.org/wiki/Q382878","display_name":"Primer extension","level":4,"score":0.27489998936653137},{"id":"https://openalex.org/C88045685","wikidata":"https://www.wikidata.org/wiki/Q174576","display_name":"Activator (genetics)","level":3,"score":0.26660001277923584}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:repozitorij.uni-lj.si:IzpisGradiva.php?id=188475","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/20.500.12556/RUL-188475","pdf_url":null,"source":{"id":"https://openalex.org/S4377196268","display_name":"Repository of the University of Ljubljana (University of Ljubljana)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I153976015","host_organization_name":"University of Ljubljana","host_organization_lineage":["https://openalex.org/I153976015"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7023817249","https://openalex.org/W3127832258","https://openalex.org/W7171725321","https://openalex.org/W4387002133","https://openalex.org/W4404325183","https://openalex.org/W4406559943","https://openalex.org/W7221480533","https://openalex.org/W4413982624","https://openalex.org/W4416365627","https://openalex.org/W4389570989"],"abstract_inverted_index":{"Molekula":[0],"informacijske":[1],"RNA":[2,129],"(mRNA,":[3],"angl.":[4,64,84,131,181],"messenger":[5],"RNA)":[6],"igra":[7],"vlogo":[8],"povezovalca":[9],"med":[10,211],"gensko":[11],"informacijo,":[12],"shranjeno":[13],"v":[14,31,39,168,286],"molekuli":[15],"DNA,":[16],"in":[17,28,61,65,73,122,144,186,214,234,245,265],"proteini,":[18],"za":[19,37,77,103,142,175,190,254,263,266,283],"katere":[20],"ta":[21],"informacija":[22],"zapisuje.":[23],"Zaradi":[24],"svojega":[25],"prehodnega":[26],"izra\u017eanja":[27],"nezmo\u017enosti":[29],"vklju\u010ditve":[30],"gostiteljski":[32],"genom":[33],"je":[34,69,139],"mRNA":[35,54,107,136,146,172,251,275],"privla\u010dna":[36],"uporabo":[38],"razli\u010dne":[40],"terapevtske":[41],"namene,":[42],"na":[43,198,249,259,272],"primer":[44],"kot":[45],"cepivo,":[46],"protirakava":[47],"imunoterapija":[48],"ali":[49],"sredstvo":[50],"celi\u010dnega":[51],"reprogramiranja.":[52],"Molekule":[53],"sinteti\u010dno":[55],"proizvajamo":[56],"s":[57,79,137,154,187],"postopkom":[58],"reakcije":[59,244],"transkripcije":[60],"vitro":[62,66],"(IVT,":[63],"transcription),":[67],"ki":[68,217],"zaradi":[70],"kompleksne":[71],"narave":[72],"visokih":[74],"stro\u0161kov":[75],"primerna":[76],"optimizacijo":[78],"pristopom":[80],"na\u010drtovanja":[81],"eksperimentov":[82,192],"(DOE,":[83],"design":[85],"of":[86],"experiments).":[87],"Pri":[88],"diplomskem":[89],"delu":[90],"smo":[91,149,162,170,240,276],"\u017eeleli":[92,150],"reakcijo":[93],"optimizirati":[94],"tako,":[95],"da":[96,203],"bi":[97],"z":[98,173,208,252,261,288],"zmanj\u0161anjem":[99],"koli\u010dine":[100],"uporabljenega":[101],"reagenta":[102,222,280],"kotranskripcijsko":[104],"pokritje":[105],"5\u2019\u2011konca":[106],"CleanCap":[108,223,281],"AG":[109,224,282],"pove\u010dali":[110],"stro\u0161kovno":[111],"u\u010dinkovitost":[112,152],"reakcije,":[113],"obenem":[114,229],"pa":[115,230,257],"ohranili":[116],"visok":[117],"dele\u017e":[118],"ustrezno":[119],"pokritega":[120],"produkta":[121],"omejili":[123],"nastanek":[124],"imunogenega":[125],"stranskega":[126],"produkta,":[127],"dvoveri\u017ene":[128],"(dsRNA,":[130],"double\u2011stranded":[132],"RNA).":[133],"Pokritje":[134],"5\u2019-konca":[135],"kapo":[138],"klju\u010dnega":[140],"pomena":[141],"kakovost":[143],"varnost":[145],"terapevtika,":[147],"zato":[148],"dose\u010di":[151],"pokritosti":[153],"5\u2019-kapo":[155],"vsaj":[156],"95":[157],"%.":[158],"Za":[159],"namen":[160],"optimizacije":[161],"izvedli":[163],"20":[164],"\u0161ar\u017enih":[165],"reakcij":[166],"IVT,":[167],"katerih":[169],"proizvedli":[171],"zapisom":[174,253,262],"oja\u010dani":[176],"zeleni":[177],"fluorescen\u010dni":[178],"protein":[179,267],"(eGFP,":[180],"enhanced":[182],"green":[183],"fluorescent":[184],"protein)":[185],"pomo\u010djo":[188],"programa":[189],"na\u010drtovanje":[191],"MODDE":[193],"preu\u010dili":[194],"vpliv":[195],"razli\u010dnih":[196],"parametrov":[197],"potek":[199],"reakcije.":[200,238],"Ugotovili":[201],"smo,":[202],"lahko":[204],"najbolj\u0161e":[205],"rezultate":[206],"dose\u017eemo":[207],"nizkim":[209],"razmerjem":[210],"koncentracijo":[212,215],"Mg2+":[213],"NTP,":[216],"nam":[218],"omogo\u010di":[219],"manj\u0161o":[220],"porabo":[221],"ob":[225],"visoki":[226],"u\u010dinkovitosti":[227],"pokritosti,":[228],"zmanj\u0161a":[231],"vsebnost":[232],"dsRNA":[233],"ne":[235],"poslab\u0161a":[236],"donosa":[237],"Dolo\u010dili":[239],"tudi":[241,258],"optimalno":[242],"to\u010dko":[243],"jo":[246],"uspe\u0161no":[247],"validirali":[248],"konstruktu":[250],"eGFP,":[255],"delno":[256],"konstruktih":[260],"luciferazo":[264],"bodice":[268],"virusa":[269],"SARS\u2011CoV\u20112.":[270],"Glede":[271],"koli\u010dino":[273],"pridobljene":[274],"dosegli":[277],"zmanj\u0161anje":[278],"porabe":[279],"25":[284],"%":[285],"primerjavi":[287],"originalnim":[289],"protokolom":[290],"proizvajalca":[291],"reagenta.":[292]},"counts_by_year":[],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2026-09-27T00:00:00"}