{"id":"https://openalex.org/W7214354752","doi":"https://doi.org/10.64898/2026.09.23.753707","title":"Daily locomotor rhythms and ecdysial stage-dependent behaviors in the tardigrade Hypsibius exemplaris","display_name":"Daily locomotor rhythms and ecdysial stage-dependent behaviors in the tardigrade Hypsibius exemplaris","publication_year":2026,"publication_date":"2026-09-25","ids":{"openalex":"https://openalex.org/W7214354752","doi":"https://doi.org/10.64898/2026.09.23.753707"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.23.753707","doi":"https://doi.org/10.64898/2026.09.23.753707","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.23.753707","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/25/2026.09.23.753707.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/25/2026.09.23.753707.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5152794809","display_name":"Baris Can Ulku","orcid":"https://orcid.org/0000-0001-7655-1026","observed_orcids":["https://orcid.org/0000-0001-7655-1026"]},"institutions":[{"id":"https://openalex.org/I106157433","display_name":"University of Kassel","ror":"https://ror.org/04zc7p361","country_code":"DE","type":"education","lineage":["https://openalex.org/I106157433"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Baris Can Ulku","raw_affiliation_strings":["Department of Zoology, Institute of Biology, University of Kassel, Germany"],"raw_orcid":"https://orcid.org/0000-0001-7655-1026","affiliations":[{"raw_affiliation_string":"Department of Zoology, Institute of Biology, University of Kassel, Germany","institution_ids":["https://openalex.org/I106157433"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061787799","display_name":"Lars Hering","orcid":"https://orcid.org/0000-0003-4469-991X","observed_orcids":["https://orcid.org/0000-0003-4469-991X"]},"institutions":[{"id":"https://openalex.org/I106157433","display_name":"University of Kassel","ror":"https://ror.org/04zc7p361","country_code":"DE","type":"education","lineage":["https://openalex.org/I106157433"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Lars Hering","raw_affiliation_strings":["Department of Zoology, Institute of Biology, University of Kassel, Germany"],"raw_orcid":"https://orcid.org/0000-0003-4469-991X","affiliations":[{"raw_affiliation_string":"Department of Zoology, Institute of Biology, University of Kassel, Germany","institution_ids":["https://openalex.org/I106157433"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026329648","display_name":"Natalia B. Filimonova","orcid":"https://orcid.org/0000-0002-5133-3003","observed_orcids":["https://orcid.org/0000-0002-5133-3003"]},"institutions":[{"id":"https://openalex.org/I106157433","display_name":"University of Kassel","ror":"https://ror.org/04zc7p361","country_code":"DE","type":"education","lineage":["https://openalex.org/I106157433"]},{"id":"https://openalex.org/I189238007","display_name":"Taras Shevchenko National University of Kyiv","ror":"https://ror.org/02aaqv166","country_code":"UA","type":"education","lineage":["https://openalex.org/I189238007"]}],"countries":["DE","UA"],"is_corresponding":false,"raw_author_name":"Natalia Filimonova","raw_affiliation_strings":["Numerical Analysis and Mathematical Modeling, Department of Mathematics, University of Kassel, Germany","Research Department, Taras Shevchenko National University of Kyiv, Ukraine"],"raw_orcid":"https://orcid.org/0000-0002-5133-3003","affiliations":[{"raw_affiliation_string":"Numerical Analysis and Mathematical Modeling, Department of Mathematics, University of Kassel, Germany","institution_ids":["https://openalex.org/I106157433"]},{"raw_affiliation_string":"Research Department, Taras Shevchenko National University of Kyiv, Ukraine","institution_ids":["https://openalex.org/I189238007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016376676","display_name":"Elfriede Friedmann","orcid":"https://orcid.org/0000-0002-2827-6581","observed_orcids":["https://orcid.org/0000-0002-2827-6581"]},"institutions":[{"id":"https://openalex.org/I106157433","display_name":"University of Kassel","ror":"https://ror.org/04zc7p361","country_code":"DE","type":"education","lineage":["https://openalex.org/I106157433"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Elfriede Friedmann","raw_affiliation_strings":["Numerical Analysis and Mathematical Modeling, Department of Mathematics, University of Kassel, Germany"],"raw_orcid":"https://orcid.org/0000-0002-2827-6581","affiliations":[{"raw_affiliation_string":"Numerical Analysis and Mathematical Modeling, Department of Mathematics, University of Kassel, Germany","institution_ids":["https://openalex.org/I106157433"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5031531070","display_name":"Georg Mayer","orcid":"https://orcid.org/0000-0003-0737-2440","observed_orcids":["https://orcid.org/0000-0003-0737-2440"]},"institutions":[{"id":"https://openalex.org/I106157433","display_name":"University of Kassel","ror":"https://ror.org/04zc7p361","country_code":"DE","type":"education","lineage":["https://openalex.org/I106157433"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Georg Mayer","raw_affiliation_strings":["Department of Zoology, Institute of Biology, University of Kassel, Germany"],"raw_orcid":"https://orcid.org/0000-0003-0737-2440","affiliations":[{"raw_affiliation_string":"Department of Zoology, Institute of Biology, University of Kassel, Germany","institution_ids":["https://openalex.org/I106157433"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5031531070"],"corresponding_institution_ids":["https://openalex.org/I106157433"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10342","display_name":"Circadian rhythm and melatonin","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/2807","display_name":"Endocrine and Autonomic Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10342","display_name":"Circadian rhythm and melatonin","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/2807","display_name":"Endocrine and Autonomic Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13679","display_name":"Tardigrade Biology and Ecology","score":0.026799999177455902,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11056","display_name":"Physiological and biochemical adaptations","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hypsibius-exemplaris","display_name":"Hypsibius exemplaris","score":0.9350000023841858},{"id":"https://openalex.org/keywords/tardigrades","display_name":"tardigrades","score":0.9539999961853027},{"id":"https://openalex.org/keywords/locomotor-activity-rhythm","display_name":"locomotor activity rhythm","score":0.7250000238418579},{"id":"https://openalex.org/keywords/circadian-rhythm","display_name":"circadian rhythm","score":0.8349999785423279},{"id":"https://openalex.org/keywords/molting","display_name":"molting","score":0.9390000104904175},{"id":"https://openalex.org/keywords/constant-darkness","display_name":"constant darkness","score":0.6320000290870667},{"id":"https://openalex.org/keywords/light-dark-cycle","display_name":"light-dark cycle","score":0.6970000267028809},{"id":"https://openalex.org/keywords/behavior-classification","display_name":"behavior classification","score":0.7480000257492065},{"id":"https://openalex.org/keywords/feeding-behavior","display_name":"feeding behavior","score":0.6010000109672546},{"id":"https://openalex.org/keywords/wandering-behavior","display_name":"wandering behavior","score":0.7639999985694885},{"id":"https://openalex.org/keywords/quiescence","display_name":"quiescence","score":0.7450000047683716},{"id":"https://openalex.org/keywords/chronobiology","display_name":"chronobiology","score":0.7770000100135803},{"id":"https://openalex.org/keywords/ecdysozoans","display_name":"ecdysozoans","score":0.8629999756813049}],"concepts":[{"id":"https://openalex.org/C135343436","wikidata":"https://www.wikidata.org/wiki/Q170406","display_name":"Rhythm","level":2,"score":0.7508000135421753},{"id":"https://openalex.org/C2993436365","wikidata":"https://www.wikidata.org/wiki/Q925958","display_name":"Locomotor activity","level":2,"score":0.6869000196456909},{"id":"https://openalex.org/C41905685","wikidata":"https://www.wikidata.org/wiki/Q381084","display_name":"Moulting","level":3,"score":0.6704999804496765},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6165000200271606},{"id":"https://openalex.org/C2777895711","wikidata":"https://www.wikidata.org/wiki/Q5194","display_name":"Tardigrade","level":2,"score":0.5885999798774719},{"id":"https://openalex.org/C2779027399","wikidata":"https://www.wikidata.org/wiki/Q204170","display_name":"Darkness","level":2,"score":0.49399998784065247},{"id":"https://openalex.org/C2908832724","wikidata":"https://www.wikidata.org/wiki/Q7187600","display_name":"Photic Stimulation","level":4,"score":0.4578999876976013},{"id":"https://openalex.org/C121446783","wikidata":"https://www.wikidata.org/wiki/Q208353","display_name":"Circadian rhythm","level":2,"score":0.41100001335144043},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.40849998593330383},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.3684000074863434},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.33739998936653137},{"id":"https://openalex.org/C187765855","wikidata":"https://www.wikidata.org/wiki/Q506874","display_name":"Chronobiology","level":3,"score":0.3140000104904175},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.31060001254081726},{"id":"https://openalex.org/C46312422","wikidata":"https://www.wikidata.org/wiki/Q11024","display_name":"Communication","level":1,"score":0.3093000054359436},{"id":"https://openalex.org/C2778163872","wikidata":"https://www.wikidata.org/wiki/Q1951705","display_name":"Muda","level":4,"score":0.3075000047683716},{"id":"https://openalex.org/C3017737102","wikidata":"https://www.wikidata.org/wiki/Q2469782","display_name":"Alpha rhythm","level":3,"score":0.27970001101493835},{"id":"https://openalex.org/C2781291010","wikidata":"https://www.wikidata.org/wiki/Q178580","display_name":"Period (music)","level":2,"score":0.27079999446868896},{"id":"https://openalex.org/C194544171","wikidata":"https://www.wikidata.org/wiki/Q21105679","display_name":"Gating","level":2,"score":0.2678999900817871}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.23.753707","doi":"https://doi.org/10.64898/2026.09.23.753707","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.23.753707","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/25/2026.09.23.753707.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.23.753707","doi":"https://doi.org/10.64898/2026.09.23.753707","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.23.753707","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/25/2026.09.23.753707.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2510115630","display_name":null,"funder_award_id":"MA 4147/12-1","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G3523770120","display_name":"Identifikation der Hauptkomponenten der EcdysteroidBiosynthese und Detektion der Ecdysteroidsynthetisierenden Gewebe bei Tardigraden, Onychophoren und Cheliceraten: Einblicke in die Evolution der H\u00e4utung bei Panarthropoden","funder_award_id":"468489906","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G4457758041","display_name":null,"funder_award_id":"GRK2749-1","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G7065877029","display_name":null,"funder_award_id":"GRK 2749","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G8344993002","display_name":"GRK 2749: Biologische Uhren auf multiplen Zeitskalen","funder_award_id":"448909517","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214354752.pdf","grobid_xml":"https://content.openalex.org/works/W7214354752.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W7154914854"],"related_works":["https://openalex.org/W2056199706","https://openalex.org/W3036342569","https://openalex.org/W2051125039","https://openalex.org/W2090936763","https://openalex.org/W1965718965","https://openalex.org/W2895346711","https://openalex.org/W4408969058","https://openalex.org/W2295633953","https://openalex.org/W1970361921","https://openalex.org/W2995534237"],"abstract_inverted_index":{"Abstract":[0],"Tardigrades,":[1],"key":[2],"ecdysozoan":[3,168],"organisms,":[4],"have":[5],"poorly":[6],"characterized":[7],"daily":[8,153],"behavioral":[9,106],"rhythms":[10],"linked":[11],"to":[12],"their":[13],"molting":[14,156,211],"(ecdysial)":[15],"cycle.":[16,194],"We":[17,54],"continuously":[18],"tracked":[19],"the":[20,24,74,78,82,192],"locomotor":[21,62,173],"activity":[22,63,111,154,174],"of":[23,70,99],"model":[25,165,203],"tardigrade":[26],"Hypsibius":[27,198],"exemplaris":[28,161,199],"using":[29],"infrared":[30],"videography":[31],"and":[32,48,81,130,136,158,188],"deep":[33],"learning-based":[34],"automated":[35],"tracking":[36],"under":[37],"three":[38],"photic":[39,71],"regimes:":[40],"a":[41,56,97,105,163,201],"light\u2013dark":[42,75],"cycle,":[43],"constant":[44,49,67],"darkness":[45,50],"following":[46],"entrainment,":[47],"without":[51,185],"prior":[52],"entrainment.":[53],"identified":[55],"robust":[57],"endogenous":[58,179],"circadian-like":[59,146,180],"rhythm":[60,79],"in":[61,66,134,149],"that":[64,151,183],"persisted":[65],"darkness,":[68],"independent":[69],"input.":[72],"Although":[73],"cycle":[76],"enhanced":[77],"amplitude":[80],"overall":[83],"animal":[84],"activity,":[85],"it":[86],"was":[87],"not":[88],"required":[89],"for":[90,166,204],"rhythmicity.":[91],"Individual":[92],"phases":[93],"varied":[94],"widely,":[95],"indicating":[96],"lack":[98],"population-level":[100],"synchronization.":[101],"Furthermore,":[102],"we":[103],"developed":[104],"classification":[107],"system":[108,148,182],"based":[109],"on":[110],"level,":[112],"which":[113],"enables":[114],"categorizing":[115],"distinct":[116,123],"behaviors.":[117],"Behavioral":[118],"patterns":[119,133],"were":[120],"modulated":[121],"by":[122,177],"ecdysial":[124,193],"stages,":[125],"with":[126,143,155,210],"quiescence":[127],"gating":[128],"premolt":[129],"time-of-day":[131],"dependent":[132],"feeding":[135],"wandering.":[137],"These":[138],"findings":[139],"provide":[140],"evidence":[141],"consistent":[142],"an":[144,178],"endogenous,":[145],"timing":[147,181],"tardigrades":[150],"integrates":[152],"cycles":[157],"establish":[159],"H.":[160],"as":[162,200],"promising":[164],"studying":[167,205],"chronobiology.":[169],"Summary":[170],"statement":[171],"Tardigrade":[172],"is":[175,189],"shaped":[176],"persists":[184],"light":[186],"input":[187],"embedded":[190],"within":[191],"This":[195],"work":[196],"establishes":[197],"tractable":[202],"how":[206],"biological":[207],"clocks":[208],"interact":[209],"programs.":[212]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-09-26T00:00:00"}