{"id":"https://openalex.org/W7214276258","doi":"https://doi.org/10.64898/2026.09.22.26363724","title":"Development and validation of a laboratory-developed test on Panther Fusion \u00ae system for detection of macrolide resistance in Bordetella pertussis","display_name":"Development and validation of a laboratory-developed test on Panther Fusion \u00ae system for detection of macrolide resistance in Bordetella pertussis","publication_year":2026,"publication_date":"2026-09-25","ids":{"openalex":"https://openalex.org/W7214276258","doi":"https://doi.org/10.64898/2026.09.22.26363724"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.22.26363724","doi":"https://doi.org/10.64898/2026.09.22.26363724","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.22.26363724","pdf_url":"https://www.medrxiv.org/content/medrxiv/early/2026/09/25/2026.09.22.26363724.full.pdf","source":{"id":"https://openalex.org/S3005729997","display_name":"medRxiv","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"medRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.medrxiv.org/content/medrxiv/early/2026/09/25/2026.09.22.26363724.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112962851","display_name":"Lamali Sadeesh Kumar","orcid":"https://orcid.org/0009-0009-4854-7906","observed_orcids":["https://orcid.org/0009-0009-4854-7906"]},"institutions":[{"id":"https://openalex.org/I165779595","display_name":"The University of Melbourne","ror":"https://ror.org/01ej9dk98","country_code":"AU","type":"education","lineage":["https://openalex.org/I165779595"]},{"id":"https://openalex.org/I4210105123","display_name":"Peter Doherty Institute","ror":"https://ror.org/016899r71","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1320473756","https://openalex.org/I165779595","https://openalex.org/I4210105123","https://openalex.org/I4210158168"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Lamali Sadeesh Kumar","raw_affiliation_strings":["Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia"],"raw_orcid":"https://orcid.org/0009-0009-4854-7906","affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]},{"raw_affiliation_string":"Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060722608","display_name":"Michelle L. Sait","orcid":"https://orcid.org/0000-0001-8782-6128","observed_orcids":["https://orcid.org/0000-0001-8782-6128"]},"institutions":[{"id":"https://openalex.org/I165779595","display_name":"The University of Melbourne","ror":"https://ror.org/01ej9dk98","country_code":"AU","type":"education","lineage":["https://openalex.org/I165779595"]},{"id":"https://openalex.org/I4210105123","display_name":"Peter Doherty Institute","ror":"https://ror.org/016899r71","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1320473756","https://openalex.org/I165779595","https://openalex.org/I4210105123","https://openalex.org/I4210158168"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Michelle Sait","raw_affiliation_strings":["Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia"],"raw_orcid":"https://orcid.org/0000-0001-8782-6128","affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]},{"raw_affiliation_string":"Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002105758","display_name":"Benjamin P. Howden","orcid":"https://orcid.org/0000-0003-0237-1473","observed_orcids":["https://orcid.org/0000-0003-0237-1473"]},"institutions":[{"id":"https://openalex.org/I165779595","display_name":"The University of Melbourne","ror":"https://ror.org/01ej9dk98","country_code":"AU","type":"education","lineage":["https://openalex.org/I165779595"]},{"id":"https://openalex.org/I2800453128","display_name":"Austin Hospital","ror":"https://ror.org/010mv7n52","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2800453128","https://openalex.org/I4210163497"]},{"id":"https://openalex.org/I4210105123","display_name":"Peter Doherty Institute","ror":"https://ror.org/016899r71","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1320473756","https://openalex.org/I165779595","https://openalex.org/I4210105123","https://openalex.org/I4210158168"]},{"id":"https://openalex.org/I4210163497","display_name":"Austin Health","ror":"https://ror.org/05dbj6g52","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210163497"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Benjamin P. Howden","raw_affiliation_strings":["Centre for Pathogen Genomics, The University of Melbourne, Melbourne, Victoria, Australia","Department of Infectious Diseases & Immunology, Austin Health, Heidelberg, Victoria, Australia","Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","WHO Collaborating Centre for Antimicrobial Resistance, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia"],"raw_orcid":"https://orcid.org/0000-0003-0237-1473","affiliations":[{"raw_affiliation_string":"Centre for Pathogen Genomics, The University of Melbourne, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595"]},{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, Austin Health, Heidelberg, Victoria, Australia","institution_ids":["https://openalex.org/I2800453128","https://openalex.org/I4210163497"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]},{"raw_affiliation_string":"Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]},{"raw_affiliation_string":"WHO Collaborating Centre for Antimicrobial Resistance, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I4210105123"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040256275","display_name":"Norelle L. Sherry","orcid":"https://orcid.org/0000-0002-7789-8360","observed_orcids":["https://orcid.org/0000-0002-7789-8360"]},"institutions":[{"id":"https://openalex.org/I165779595","display_name":"The University of Melbourne","ror":"https://ror.org/01ej9dk98","country_code":"AU","type":"education","lineage":["https://openalex.org/I165779595"]},{"id":"https://openalex.org/I2800453128","display_name":"Austin Hospital","ror":"https://ror.org/010mv7n52","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2800453128","https://openalex.org/I4210163497"]},{"id":"https://openalex.org/I4210105123","display_name":"Peter Doherty Institute","ror":"https://ror.org/016899r71","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1320473756","https://openalex.org/I165779595","https://openalex.org/I4210105123","https://openalex.org/I4210158168"]},{"id":"https://openalex.org/I4210163497","display_name":"Austin Health","ror":"https://ror.org/05dbj6g52","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210163497"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Norelle L. Sherry","raw_affiliation_strings":["Department of Infectious Diseases & Immunology, Austin Health, Heidelberg, Victoria, Australia","Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","WHO Collaborating Centre for Antimicrobial Resistance, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia"],"raw_orcid":"https://orcid.org/0000-0002-7789-8360","affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, Austin Health, Heidelberg, Victoria, Australia","institution_ids":["https://openalex.org/I2800453128","https://openalex.org/I4210163497"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]},{"raw_affiliation_string":"Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I165779595","https://openalex.org/I4210105123"]},{"raw_affiliation_string":"WHO Collaborating Centre for Antimicrobial Resistance, The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia","institution_ids":["https://openalex.org/I4210105123"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5040256275"],"corresponding_institution_ids":["https://openalex.org/I165779595","https://openalex.org/I2800453128","https://openalex.org/I4210105123","https://openalex.org/I4210163497"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.5551999807357788,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.5551999807357788,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.4171999990940094,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.007899999618530273,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bordetella-pertussis","display_name":"Bordetella pertussis","score":0.9430000185966492},{"id":"https://openalex.org/keywords/macrolide-resistance","display_name":"macrolide resistance","score":0.9879999756813049},{"id":"https://openalex.org/keywords/23s-rrna-gene","display_name":"23S rRNA gene","score":0.9950000047683716},{"id":"https://openalex.org/keywords/laboratory-developed-tests","display_name":"laboratory-developed tests","score":0.8970000147819519},{"id":"https://openalex.org/keywords/real-time-pcr","display_name":"real-time PCR","score":0.8949999809265137},{"id":"https://openalex.org/keywords/assay-validation","display_name":"assay validation","score":0.734000027179718},{"id":"https://openalex.org/keywords/reflex-testing","display_name":"reflex testing","score":0.6050000190734863},{"id":"https://openalex.org/keywords/pertussis","display_name":"pertussis","score":0.7049999833106995},{"id":"https://openalex.org/keywords/antimicrobial-susceptibility-testing","display_name":"antimicrobial susceptibility testing","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C2777582894","wikidata":"https://www.wikidata.org/wiki/Q137103","display_name":"Bordetella pertussis","level":3,"score":0.8198000192642212},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5734999775886536},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5382999777793884},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46320000290870667},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.45739999413490295},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.454800009727478},{"id":"https://openalex.org/C94665300","wikidata":"https://www.wikidata.org/wiki/Q63391344","display_name":"Antibiotic resistance","level":3,"score":0.4043999910354614},{"id":"https://openalex.org/C40749294","wikidata":"https://www.wikidata.org/wiki/Q4631553","display_name":"23S ribosomal RNA","level":5,"score":0.39899998903274536},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.3783999979496002},{"id":"https://openalex.org/C114851261","wikidata":"https://www.wikidata.org/wiki/Q12147416","display_name":"Drug resistance","level":2,"score":0.35740000009536743},{"id":"https://openalex.org/C2779214499","wikidata":"https://www.wikidata.org/wiki/Q134859","display_name":"Whooping cough","level":3,"score":0.35370001196861267},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.33660000562667847},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3240000009536743},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.3151000142097473},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.3012999892234802},{"id":"https://openalex.org/C2779634000","wikidata":"https://www.wikidata.org/wiki/Q7170955","display_name":"Pertussis vaccine","level":4,"score":0.2825999855995178},{"id":"https://openalex.org/C2994225774","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Gene mutation","level":4,"score":0.28220000863075256},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.27219998836517334},{"id":"https://openalex.org/C2992965680","wikidata":"https://www.wikidata.org/wiki/Q598976","display_name":"Clinical microbiology","level":2,"score":0.27059999108314514},{"id":"https://openalex.org/C2777824465","wikidata":"https://www.wikidata.org/wiki/Q893440","display_name":"Bordetella","level":4,"score":0.2648000121116638},{"id":"https://openalex.org/C180223184","wikidata":"https://www.wikidata.org/wiki/Q4839986","display_name":"Bacterial genetics","level":4,"score":0.2644999921321869},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.26440000534057617},{"id":"https://openalex.org/C143660080","wikidata":"https://www.wikidata.org/wiki/Q7684883","display_name":"TaqMan","level":4,"score":0.25999999046325684},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.2533999979496002},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.2531000077724457}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.22.26363724","doi":"https://doi.org/10.64898/2026.09.22.26363724","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.22.26363724","pdf_url":"https://www.medrxiv.org/content/medrxiv/early/2026/09/25/2026.09.22.26363724.full.pdf","source":{"id":"https://openalex.org/S3005729997","display_name":"medRxiv","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"medRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.22.26363724","doi":"https://doi.org/10.64898/2026.09.22.26363724","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.22.26363724","pdf_url":"https://www.medrxiv.org/content/medrxiv/early/2026/09/25/2026.09.22.26363724.full.pdf","source":{"id":"https://openalex.org/S3005729997","display_name":"medRxiv","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"medRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320315075","display_name":"Western Sydney Local Health District","ror":"https://ror.org/01vqqp163"},{"id":"https://openalex.org/F4320322858","display_name":"State Government of Victoria","ror":"https://ror.org/02xvc5r05"},{"id":"https://openalex.org/F4320334705","display_name":"National Health and Medical Research Council","ror":"https://ror.org/011kf5r70"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214276258.pdf","grobid_xml":"https://content.openalex.org/works/W7214276258.grobid-xml"},"referenced_works_count":12,"referenced_works":["https://openalex.org/W1004806948","https://openalex.org/W1881226139","https://openalex.org/W2109875381","https://openalex.org/W2214941520","https://openalex.org/W2606988227","https://openalex.org/W2925222194","https://openalex.org/W4390030948","https://openalex.org/W4408335096","https://openalex.org/W4412023587","https://openalex.org/W4416227335","https://openalex.org/W7131384821","https://openalex.org/W7162649239"],"related_works":["https://openalex.org/W7222997518","https://openalex.org/W4214657903","https://openalex.org/W7123339171","https://openalex.org/W7081439030","https://openalex.org/W7202111105","https://openalex.org/W1881226139","https://openalex.org/W4404053042","https://openalex.org/W4408335096","https://openalex.org/W4313887367","https://openalex.org/W7131384821"],"abstract_inverted_index":{"Abstract":[0],"Macrolides":[1],"remain":[2],"the":[3,51,96,106,126,136,158,171,178,219],"first-line":[4],"antimicrobial":[5,44],"treatment":[6],"for":[7,28,35,43,73,125,132,215],"pertussis,":[8,36],"primarily":[9],"caused":[10],"by":[11],"Bordetella":[12],"pertussis":[13,19,64,112,228,244],".":[14,229],"Emergence":[15],"of":[16,39,143,152,193,195,218,242,249],"macrolide-resistant":[17],"B.":[18,63,111,227,243],"in":[20,50,62,110,135,157,170,226],"Australia":[21],"and":[22,81,116,129,167,186,211],"globally":[23],"presents":[24],"a":[25,83,90,191,209,237],"growing":[26],"concern":[27],"clinical":[29,117,168],"management.":[30],"As":[31],"molecular":[32,69,216,234],"diagnosis":[33],"predominates":[34],"limited":[37],"numbers":[38],"cultures":[40],"are":[41],"available":[42,72],"susceptibility":[45],"testing.":[46],"The":[47,188],"A2037G":[48],"mutation":[49,109,180,221],"23S":[52],"rRNA":[53],"gene":[54],"is":[55,71],"most":[56],"commonly":[57],"associated":[58,222],"with":[59,139,177,223],"macrolide":[60,107,224],"resistance":[61,225],",":[65],"yet":[66],"no":[67],"commercial":[68],"assay":[70,88,163,189],"its":[74],"detection.":[75],"In":[76],"this":[77],"study,":[78],"we":[79],"adapted":[80],"validated":[82],"previously":[84],"published":[85],"real-time":[86],"PCR":[87],"as":[89,236],"laboratory":[91],"developed":[92],"test":[93,239],"(LDT)":[94],"on":[95],"Panther":[97,204],"Fusion":[98,205],"\u00ae":[99,206],"system":[100],"using":[101],"Open":[102],"Access\u2212functionality":[103],"to":[104,161],"detect":[105],"resistance-associated":[108],"from":[113],"culture":[114],"isolates":[115,166],"specimens.":[118],"Total":[119],"turnaround":[120],"time":[121],"was":[122],"\u223c2.5":[123],"h":[124],"first":[127],"result":[128],"2-3":[130],"min":[131],"subsequent":[133],"results":[134,175],"same":[137],"batch,":[138],"an":[140],"estimated":[141],"cost":[142],"AUD":[144],"18":[145],"per":[146],"sample,":[147],"excluding":[148],"labour.":[149],"A":[150],"total":[151],"30":[153],"samples":[154],"were":[155],"included":[156],"validation":[159,172],"panel":[160,173],"assess":[162],"performance.":[164],"All":[165],"specimens":[169],"produced":[174],"concordant":[176],"expected":[179],"status,":[181],"demonstrating":[182],"100%":[183],"accuracy,":[184],"sensitivity":[185],"specificity.":[187],"demonstrated":[190],"limit":[192],"detection":[194,217],"1.5":[196],"\u00d7":[197],"10":[198],"3":[199],"CFU/mL":[200],"(22":[201],"CFU/reaction).":[202],"This":[203],"LDT":[207],"provides":[208],"rapid":[210],"fully":[212],"automated":[213],"approach":[214],"targeted":[220],"Its":[230],"integration":[231],"into":[232],"routine":[233],"workflows":[235],"reflex":[238],"following":[240],"identification":[241,248],"may":[245],"facilitate":[246],"timely":[247],"resistant":[250],"infections.":[251]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-26T00:00:00"}