{"id":"https://openalex.org/W7214152736","doi":"https://doi.org/10.64898/2026.09.23.753728","title":"Multi-attribute characterisation of mRNA via MazF endoribonuclease and LC-MS workflows","display_name":"Multi-attribute characterisation of mRNA via MazF endoribonuclease and LC-MS workflows","publication_year":2026,"publication_date":"2026-09-24","ids":{"openalex":"https://openalex.org/W7214152736","doi":"https://doi.org/10.64898/2026.09.23.753728"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.23.753728","doi":"https://doi.org/10.64898/2026.09.23.753728","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.23.753728","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/24/2026.09.23.753728.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/24/2026.09.23.753728.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5152679355","display_name":"Emma P Welbourne","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I91136226","display_name":"University of Sheffield","ror":"https://ror.org/05krs5044","country_code":"GB","type":"education","lineage":["https://openalex.org/I91136226"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Emma. N. Welbourne","raw_affiliation_strings":["School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK","institution_ids":["https://openalex.org/I91136226"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041015784","display_name":"Gareth R. Owen","orcid":"https://orcid.org/0000-0002-8695-757X","observed_orcids":["https://orcid.org/0000-0002-8695-757X"]},"institutions":[{"id":"https://openalex.org/I91136226","display_name":"University of Sheffield","ror":"https://ror.org/05krs5044","country_code":"GB","type":"education","lineage":["https://openalex.org/I91136226"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Gareth R. Owen","raw_affiliation_strings":["School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK","institution_ids":["https://openalex.org/I91136226"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5152651017","display_name":"Lauren P Wright","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I91136226","display_name":"University of Sheffield","ror":"https://ror.org/05krs5044","country_code":"GB","type":"education","lineage":["https://openalex.org/I91136226"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Lauren P. Wright","raw_affiliation_strings":["School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK","institution_ids":["https://openalex.org/I91136226"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052457337","display_name":"Sara Maria David Trabulo","orcid":"https://orcid.org/0000-0002-3178-2119","observed_orcids":["https://orcid.org/0000-0002-3178-2119"]},"institutions":[{"id":"https://openalex.org/I105036370","display_name":"AstraZeneca (United Kingdom)","ror":"https://ror.org/04r9x1a08","country_code":"GB","type":"company","lineage":["https://openalex.org/I105036370"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sara Trabulo","raw_affiliation_strings":["Analytical Sciences, BioPharmaceuticals Development, BioPharmaceuticals R&D, AstraZeneca, Cambridge Biomedical Campus, Cambridge CB2 0AA, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Analytical Sciences, BioPharmaceuticals Development, BioPharmaceuticals R&D, AstraZeneca, Cambridge Biomedical Campus, Cambridge CB2 0AA, United Kingdom","institution_ids":["https://openalex.org/I105036370"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5128773248","display_name":"Zoltan Kis","orcid":"https://orcid.org/0000-0002-6598-8369","observed_orcids":["https://orcid.org/0000-0002-6598-8369"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]},{"id":"https://openalex.org/I91136226","display_name":"University of Sheffield","ror":"https://ror.org/05krs5044","country_code":"GB","type":"education","lineage":["https://openalex.org/I91136226"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Zolt\u00e1n Kis","raw_affiliation_strings":["Department of Chemical Engineering, Imperial College London, London, UK","School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Imperial College London, London, UK","institution_ids":["https://openalex.org/I47508984"]},{"raw_affiliation_string":"School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK","institution_ids":["https://openalex.org/I91136226"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037080909","display_name":"Mark J. Dickman","orcid":"https://orcid.org/0000-0002-9236-0788","observed_orcids":["https://orcid.org/0000-0002-9236-0788"]},"institutions":[{"id":"https://openalex.org/I91136226","display_name":"University of Sheffield","ror":"https://ror.org/05krs5044","country_code":"GB","type":"education","lineage":["https://openalex.org/I91136226"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Mark J. Dickman","raw_affiliation_strings":["School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK"],"raw_orcid":"https://orcid.org/0000-0002-9236-0788","affiliations":[{"raw_affiliation_string":"School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK","institution_ids":["https://openalex.org/I91136226"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5037080909"],"corresponding_institution_ids":["https://openalex.org/I91136226"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.4458000063896179,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.4458000063896179,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.1509000062942505,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0608999989926815,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/site-specific-rna-cleavage","display_name":"site-specific RNA cleavage","score":0.6299999952316284},{"id":"https://openalex.org/keywords/poly-a-tail-length","display_name":"poly(A) tail length","score":0.9490000009536743},{"id":"https://openalex.org/keywords/mrna-sequencing","display_name":"mRNA sequencing","score":0.6340000033378601},{"id":"https://openalex.org/keywords/mrna-vaccines","display_name":"mRNA vaccines","score":0.7020000219345093},{"id":"https://openalex.org/keywords/mrna-therapeutics","display_name":"mRNA therapeutics","score":0.9340000152587891},{"id":"https://openalex.org/keywords/critical-quality-attributes","display_name":"critical quality attributes","score":0.7049999833106995},{"id":"https://openalex.org/keywords/rnase-t1","display_name":"RNase T1","score":0.8370000123977661}],"concepts":[{"id":"https://openalex.org/C2780102198","wikidata":"https://www.wikidata.org/wiki/Q3054027","display_name":"Endoribonuclease","level":5,"score":0.9406999945640564},{"id":"https://openalex.org/C10879258","wikidata":"https://www.wikidata.org/wiki/Q76826611","display_name":"RNase P","level":4,"score":0.7572000026702881},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.6518999934196472},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6291000247001648},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.5365999937057495},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.49559998512268066},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.48739999532699585},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43860000371932983},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.43689998984336853},{"id":"https://openalex.org/C105130999","wikidata":"https://www.wikidata.org/wiki/Q414976","display_name":"Pseudoknot","level":4,"score":0.4156999886035919},{"id":"https://openalex.org/C69766542","wikidata":"https://www.wikidata.org/wiki/Q3430179","display_name":"RNase H","level":5,"score":0.40389999747276306},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39500001072883606},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.35760000348091125},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.35339999198913574},{"id":"https://openalex.org/C10010492","wikidata":"https://www.wikidata.org/wiki/Q3142557","display_name":"Protein sequencing","level":4,"score":0.32850000262260437},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.31929999589920044},{"id":"https://openalex.org/C79750893","wikidata":"https://www.wikidata.org/wiki/Q3430180","display_name":"Ribonuclease III","level":5,"score":0.31299999356269836},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3066999912261963},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.30660000443458557},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.30640000104904175},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.2881999909877777},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.2856000065803528},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.27250000834465027}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.23.753728","doi":"https://doi.org/10.64898/2026.09.23.753728","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.23.753728","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/24/2026.09.23.753728.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.23.753728","doi":"https://doi.org/10.64898/2026.09.23.753728","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.23.753728","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/24/2026.09.23.753728.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8639509881","display_name":"Electrospun mucoadhesive matrices for polymersome-mediated mRNA vaccine delivery","funder_award_id":"BB/Y007514/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320314731","display_name":"UK Research and Innovation","ror":"https://ror.org/001aqnf71"},{"id":"https://openalex.org/F4320316507","display_name":"Coalition for Epidemic Preparedness Innovations","ror":"https://ror.org/02j9wvt50"},{"id":"https://openalex.org/F4320332167","display_name":"Directorate for Biological Sciences","ror":"https://ror.org/001xhss06"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214152736.pdf","grobid_xml":"https://content.openalex.org/works/W7214152736.grobid-xml"},"referenced_works_count":24,"referenced_works":["https://openalex.org/W2083873972","https://openalex.org/W2095950646","https://openalex.org/W2124854998","https://openalex.org/W2154964144","https://openalex.org/W2339574870","https://openalex.org/W2564791984","https://openalex.org/W2945939284","https://openalex.org/W2986812831","https://openalex.org/W3047091198","https://openalex.org/W3111255098","https://openalex.org/W3127832258","https://openalex.org/W3201215980","https://openalex.org/W4214669202","https://openalex.org/W4280575507","https://openalex.org/W4281254240","https://openalex.org/W4286797678","https://openalex.org/W4313236460","https://openalex.org/W4379259615","https://openalex.org/W4387429772","https://openalex.org/W4391958084","https://openalex.org/W4396945872","https://openalex.org/W4400747031","https://openalex.org/W4406917187","https://openalex.org/W4406998049"],"related_works":["https://openalex.org/W4388291501","https://openalex.org/W4280575507","https://openalex.org/W7140126433","https://openalex.org/W4406998049","https://openalex.org/W2945939284","https://openalex.org/W4313236460","https://openalex.org/W4391134258","https://openalex.org/W7165636836","https://openalex.org/W4406917187","https://openalex.org/W4396904297"],"abstract_inverted_index":{"Abstract":[0],"The":[1],"rapid":[2,68],"expansion":[3],"of":[4,16,81,108,128,189],"mRNA-based":[5],"medicines":[6],"has":[7],"driven":[8],"demand":[9],"for":[10,43,179],"robust,":[11],"high-resolution":[12],"analytical":[13],"methods":[14],"capable":[15],"characterising":[17],"critical":[18],"quality":[19,182],"attributes":[20],"including":[21],"sequence":[22,76,106,136,148,187],"identity,":[23],"5\u2019":[24],"capping":[25],"efficiency,":[26],"and":[27,31,78,84,88,102,111,131,166,184,192],"poly(A)":[28],"tail":[29,86],"length":[30,87],"heterogeneity.":[32,89],"Here,":[33],"we":[34],"present":[35],"two":[36],"complementary":[37,115],"liquid":[38],"chromatography-mass":[39],"spectrometry":[40],"based":[41,47,150],"workflows":[42,173],"mRNA":[44,98,135,190],"characterisation,":[45],"both":[46,180],"on":[48,151],"site-specific":[49],"digestion":[50],"with":[51,154],"the":[52],"endoribonuclease":[53],"MazF":[54,155],"from":[55],"Escherichia":[56],"coli":[57],".":[58],"A":[59,114],"\u201cmiddle-up\u201d":[60],"approach,":[61,117],"optimised":[62],"via":[63,70,134],"specific":[64,120],"\u201c5\u2019-ACA\u201d":[65],"cleavage":[66,122],"enables":[67,123],"characterisation":[69,133,188],"oligoribonucleotide":[71],"mass":[72,125],"mapping":[73],"providing":[74],"complete":[75],"coverage":[77,149],"simultaneous":[79],"assessment":[80,183],"5-capping":[82],"efficiency":[83],"3\u2019-poly(A)":[85],"This":[90],"workflow":[91],"was":[92],"successfully":[93],"applied":[94],"to":[95,139,158],"three":[96],"different":[97],"constructs,":[99],"NLuc,":[100],"eGFP":[101],"FLuc":[103],"mRNAs,":[104],"achieving":[105],"coverages":[107],"100%,":[109],"87%,":[110],"74%,":[112],"respectively.":[113],"\u201cbottom-up\u201d":[116],"employing":[118],"less":[119],"\u201c5\u2019-ACX\u201d":[121],"tandem":[124],"spectrometry-based":[126],"sequencing":[127],"shorter":[129],"oligoribonucleotides":[130,153],"detailed":[132],"mapping.":[137],"Applied":[138],"SARS-CoV-2":[140],"Spike":[141],"Protein":[142],"mRNA,":[143],"this":[144],"method":[145],"yielded":[146],"50%":[147],"unique":[152],"alone,":[156],"extended":[157],"90%":[159],"by":[160],"combining":[161],"MazF,":[162],"partial":[163,167],"RNase":[164,168],"T1,":[165],"U2":[169],"digests.":[170],"Together,":[171],"these":[172],"provide":[174],"a":[175],"flexible,":[176],"orthogonal":[177],"platform":[178],"high-throughput":[181],"in-depth":[185],"primary":[186],"vaccines":[191],"therapeutics.":[193]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-09-25T00:00:00"}
