{"id":"https://openalex.org/W7214065860","doi":"https://doi.org/10.64898/2026.09.22.753519","title":"Oxaliplatin and 5-Fluorouracil induce p53-p21-pRb-associated cell cycle arrest and a transient senescence-like phenotype in patient-derived low-grade serous ovarian cancer cells","display_name":"Oxaliplatin and 5-Fluorouracil induce p53-p21-pRb-associated cell cycle arrest and a transient senescence-like phenotype in patient-derived low-grade serous ovarian cancer cells","publication_year":2026,"publication_date":"2026-09-23","ids":{"openalex":"https://openalex.org/W7214065860","doi":"https://doi.org/10.64898/2026.09.22.753519"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.22.753519","doi":"https://doi.org/10.64898/2026.09.22.753519","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.22.753519","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/23/2026.09.22.753519.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/23/2026.09.22.753519.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089753544","display_name":"Rewati Prakash","orcid":"https://orcid.org/0009-0005-2098-6561","observed_orcids":["https://orcid.org/0009-0005-2098-6561"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Rewati Prakash","raw_affiliation_strings":["Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012841376","display_name":"Benjamin N. Forgie","orcid":"https://orcid.org/0009-0003-7272-2141","observed_orcids":["https://orcid.org/0009-0003-7272-2141"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Benjamin N Forgie","raw_affiliation_strings":["Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada"],"raw_orcid":"https://orcid.org/0009-0003-7272-2141","affiliations":[{"raw_affiliation_string":"Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004596792","display_name":"Alicia Alejandra Goyeneche","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801766832","display_name":"McGill University Health Centre","ror":"https://ror.org/04cpxjv19","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I4405263283","display_name":"Research Institute of the McGill University Health Centre","ror":"https://ror.org/04pemf943","country_code":"CA","type":"government","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405263283","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Alicia A Goyeneche","raw_affiliation_strings":["Cancer Research Program, Research Institute, McGill University Health Centre, Montreal, QC, Canada","Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cancer Research Program, Research Institute, McGill University Health Centre, Montreal, QC, Canada","institution_ids":["https://openalex.org/I2801766832","https://openalex.org/I4405263283"]},{"raw_affiliation_string":"Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004185032","display_name":"Edith Zorychta","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Edith Zorychta","raw_affiliation_strings":["Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5152503727","display_name":"Abu Noman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I894293524","display_name":"University of Chittagong","ror":"https://ror.org/01173vs27","country_code":"BD","type":"education","lineage":["https://openalex.org/I894293524"]}],"countries":["BD"],"is_corresponding":false,"raw_author_name":"Abu Shadat M Noman","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, Chittagong University, Chittagong, Bangladesh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, Chittagong University, Chittagong, Bangladesh","institution_ids":["https://openalex.org/I894293524"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069269249","display_name":"Lucy Gilbert","orcid":"https://orcid.org/0000-0001-8605-5472","observed_orcids":["https://orcid.org/0000-0001-8605-5472"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Lucy Gilbert","raw_affiliation_strings":["Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008149071","display_name":"Carlos Marcelo Telleria","orcid":"https://orcid.org/0000-0001-8799-9010","observed_orcids":["https://orcid.org/0000-0001-8799-9010","https://orcid.org/0000-0003-1070-3538"]},"institutions":[{"id":"https://openalex.org/I2801766832","display_name":"McGill University Health Centre","ror":"https://ror.org/04cpxjv19","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I4405263283","display_name":"Research Institute of the McGill University Health Centre","ror":"https://ror.org/04pemf943","country_code":"CA","type":"government","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405263283","https://openalex.org/I4405279980"]},{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Carlos M Telleria","raw_affiliation_strings":["Cancer Research Program, Research Institute, McGill University Health Centre, Montreal, QC, Canada","Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada","Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada"],"raw_orcid":"https://orcid.org/0000-0003-1070-3538","affiliations":[{"raw_affiliation_string":"Cancer Research Program, Research Institute, McGill University Health Centre, Montreal, QC, Canada","institution_ids":["https://openalex.org/I2801766832","https://openalex.org/I4405263283"]},{"raw_affiliation_string":"Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]},{"raw_affiliation_string":"Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada","institution_ids":["https://openalex.org/I5023651"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5008149071"],"corresponding_institution_ids":["https://openalex.org/I2801766832","https://openalex.org/I4405263283","https://openalex.org/I5023651"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10550","display_name":"Ovarian cancer diagnosis and treatment","score":0.9785000085830688,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10550","display_name":"Ovarian cancer diagnosis and treatment","score":0.9785000085830688,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.013899999670684338,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.0044999998062849045,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/low-grade-serous-ovarian-carcinoma","display_name":"low-grade serous ovarian cancer","score":0.9850000143051147},{"id":"https://openalex.org/keywords/oxaliplatin","display_name":"oxaliplatin","score":1.0},{"id":"https://openalex.org/keywords/5-fluorouracil","display_name":"5-fluorouracil","score":1.0},{"id":"https://openalex.org/keywords/cell-cycle-arrest","display_name":"cell cycle arrest","score":0.9440000057220459},{"id":"https://openalex.org/keywords/g0-g1-arrest","display_name":"G0/G1 arrest","score":0.8899999856948853},{"id":"https://openalex.org/keywords/senescence-associated-beta-galactosidase","display_name":"senescence-associated \u03b2-galactosidase","score":0.9449999928474426},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.8629999756813049},{"id":"https://openalex.org/keywords/wild-type-p53","display_name":"wild-type p53","score":0.7310000061988831},{"id":"https://openalex.org/keywords/chemosensitization","display_name":"chemosensitization","score":0.6809999942779541}],"concepts":[{"id":"https://openalex.org/C2780962732","wikidata":"https://www.wikidata.org/wiki/Q422327","display_name":"Oxaliplatin","level":4,"score":0.8852999806404114},{"id":"https://openalex.org/C117262875","wikidata":"https://www.wikidata.org/wiki/Q5135057","display_name":"Clonogenic assay","level":3,"score":0.6625999808311462},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.6215000152587891},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.5697000026702881},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.5446000099182129},{"id":"https://openalex.org/C150173356","wikidata":"https://www.wikidata.org/wiki/Q6144101","display_name":"Serous fluid","level":2,"score":0.5285999774932861},{"id":"https://openalex.org/C105696609","wikidata":"https://www.wikidata.org/wiki/Q1068809","display_name":"Cell cycle checkpoint","level":4,"score":0.5177000164985657},{"id":"https://openalex.org/C2780427987","wikidata":"https://www.wikidata.org/wiki/Q172341","display_name":"Ovarian cancer","level":3,"score":0.4997999966144562},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.4733999967575073},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.43130001425743103},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3693999946117401},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.3684000074863434},{"id":"https://openalex.org/C25311885","wikidata":"https://www.wikidata.org/wiki/Q306383","display_name":"G1 phase","level":5,"score":0.3630000054836273},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.36149999499320984},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.35740000009536743},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.351500004529953},{"id":"https://openalex.org/C2776694085","wikidata":"https://www.wikidata.org/wiki/Q974135","display_name":"Chemotherapy","level":2,"score":0.3352000117301941},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.31940001249313354},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.31060001254081726},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.30379998683929443},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3027999997138977},{"id":"https://openalex.org/C2777389519","wikidata":"https://www.wikidata.org/wiki/Q425088","display_name":"Temozolomide","level":3,"score":0.30219998955726624},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.2992999851703644},{"id":"https://openalex.org/C522857546","wikidata":"https://www.wikidata.org/wiki/Q2070979","display_name":"Senescence","level":2,"score":0.28859999775886536},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.27950000762939453},{"id":"https://openalex.org/C2781451048","wikidata":"https://www.wikidata.org/wiki/Q415588","display_name":"Carboplatin","level":4,"score":0.2565000057220459},{"id":"https://openalex.org/C182979987","wikidata":"https://www.wikidata.org/wiki/Q3907241","display_name":"Poly ADP ribose polymerase","level":4,"score":0.2531999945640564},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.25029999017715454}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.22.753519","doi":"https://doi.org/10.64898/2026.09.22.753519","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.22.753519","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/23/2026.09.22.753519.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.22.753519","doi":"https://doi.org/10.64898/2026.09.22.753519","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.22.753519","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/23/2026.09.22.753519.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.98580002784729}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310638","display_name":"McGill University","ror":"https://ror.org/01pxwe438"},{"id":"https://openalex.org/F4320317255","display_name":"Ovarian Cancer Canada","ror":"https://ror.org/024kejf30"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7214065860.pdf","grobid_xml":"https://content.openalex.org/works/W7214065860.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4409690342","https://openalex.org/W4392377811","https://openalex.org/W3152905220","https://openalex.org/W3206022180","https://openalex.org/W2902300797","https://openalex.org/W2044338399","https://openalex.org/W2057368164","https://openalex.org/W2140997761","https://openalex.org/W2315283231","https://openalex.org/W2538145882"],"abstract_inverted_index":{"Abstract":[0],"Objectives":[1],"Low-grade":[2],"serous":[3],"ovarian":[4],"cancer":[5],"(LGSOC)":[6],"is":[7],"characterized":[8],"by":[9,85,160],"frequent":[10],"TP53":[11],"wild-type":[12],"status":[13],"and":[14,43,57,75,80,105,107,151,157],"limited":[15],"responsiveness":[16],"to":[17],"conventional":[18],"chemotherapy.":[19],"This":[20],"study":[21],"investigated":[22],"the":[23,68,72,93,129,162],"cytotoxic":[24],"effects":[25,47],"of":[26,41,65,71,78,92],"oxaliplatin":[27],"(OXP)":[28],"combined":[29],"with":[30,90],"5-fluorouracil":[31],"(5-FU)":[32],"on":[33,48],"patient-derived":[34],"LGSOC":[35,49,149],"cells.":[36],"Results":[37],"Clinically":[38],"relevant":[39],"concentrations":[40],"OXP":[42],"5-FU":[44],"had":[45],"minimal":[46],"cell":[50,73],"viability":[51],"but":[52],"markedly":[53],"reduced":[54,86],"cellular":[55,103],"proliferation":[56],"clonogenic":[58],"recovery.":[59],"Combined":[60],"treatment":[61],"induced":[62],"pronounced":[63],"accumulation":[64],"cells":[66,122,150],"in":[67],"G0/G1":[69],"phase":[70],"cycle":[74],"increased":[76,98],"expression":[77],"p53":[79],"p21":[81],"Cip1":[82],",":[83],"accompanied":[84],"pRb":[87],"phosphorylation,":[88],"consistent":[89],"activation":[91],"p53\u2013p21\u2013pRb":[94,163],"pathway.":[95,164],"OXP+5-FU":[96,143],"also":[97],"senescence-associated":[99],"\u03b2-galactosidase":[100],"(SA-\u03b2-Gal)":[101],"activity,":[102],"enlargement":[104],"flattening,":[106],"reactive":[108],"oxygen":[109],"species":[110],"(ROS)":[111],"production,":[112],"supporting":[113],"a":[114,153],"senescence-like":[115,155],"phenotype.":[116],"However,":[117],"after":[118],"drug":[119],"withdrawal,":[120],"treated":[121],"progressively":[123],"regained":[124],"proliferative":[125],"capacity,":[126],"indicating":[127],"that":[128,142],"senescence":[130],"phenotype":[131,156],"was":[132],"transient":[133,154],"rather":[134,146],"than":[135,147],"fully":[136],"irreversible.":[137],"Together,":[138],"these":[139],"findings":[140],"show":[141],"primarily":[144],"arrests":[145],"kills":[148],"induces":[152],"G0/G1-phase":[158],"arrest":[159],"activating":[161]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-09-24T00:00:00"}