{"id":"https://openalex.org/W7213985207","doi":"https://doi.org/10.64898/2026.09.20.753044","title":"Optimization of a Human Anti-polio Monoclonal Antibody as a Potential Therapeutic Modality","display_name":"Optimization of a Human Anti-polio Monoclonal Antibody as a Potential Therapeutic Modality","publication_year":2026,"publication_date":"2026-09-22","ids":{"openalex":"https://openalex.org/W7213985207","doi":"https://doi.org/10.64898/2026.09.20.753044"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.20.753044","doi":"https://doi.org/10.64898/2026.09.20.753044","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.20.753044","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/22/2026.09.20.753044.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/22/2026.09.20.753044.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064255255","display_name":"Shwu\u2010Maan Lee","orcid":"https://orcid.org/0000-0003-0819-0569","observed_orcids":["https://orcid.org/0000-0003-0819-0569"]},"institutions":[{"id":"https://openalex.org/I1304598786","display_name":"Program for Appropriate Technology in Health","ror":"https://ror.org/02ycvrx49","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1304598786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shwu-Maan Lee","raw_affiliation_strings":["PATH Center for Vaccine Innovation and Access, Seattle, Washington, United States"],"raw_orcid":"https://orcid.org/0000-0003-0819-0569","affiliations":[{"raw_affiliation_string":"PATH Center for Vaccine Innovation and Access, Seattle, Washington, United States","institution_ids":["https://openalex.org/I1304598786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029718173","display_name":"Christine C. Siska","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Christine Siska","raw_affiliation_strings":["Just-Evotec Biologics, Seattle, Washington, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Just-Evotec Biologics, Seattle, Washington, United States","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030082552","display_name":"Igor D\u2019Angelo","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Igor D\u2019Angelo","raw_affiliation_strings":["Just-Evotec Biologics, Seattle, Washington, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Just-Evotec Biologics, Seattle, Washington, United States","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049094904","display_name":"Diana V. Kouiavskaia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1318287680","display_name":"Center for Biologics Evaluation and Research","ror":"https://ror.org/02nr3fr97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1318287680","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Diana Kouiavskaia","raw_affiliation_strings":["Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, United States","institution_ids":["https://openalex.org/I1318287680","https://openalex.org/I1320320070"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070763847","display_name":"Kutub Mahmood","orcid":"https://orcid.org/0000-0002-6181-8178","observed_orcids":["https://orcid.org/0000-0002-6181-8178"]},"institutions":[{"id":"https://openalex.org/I1304598786","display_name":"Program for Appropriate Technology in Health","ror":"https://ror.org/02ycvrx49","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1304598786"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kutub Mahmood","raw_affiliation_strings":["PATH Center for Vaccine Innovation and Access, Seattle, Washington, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"PATH Center for Vaccine Innovation and Access, Seattle, Washington, United States","institution_ids":["https://openalex.org/I1304598786"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5070763847"],"corresponding_institution_ids":["https://openalex.org/I1304598786"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.011699999682605267,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/poliovirus","display_name":"poliovirus","score":0.8640000224113464},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8460000157356262},{"id":"https://openalex.org/keywords/antibody-engineering","display_name":"antibody engineering","score":0.492000013589859},{"id":"https://openalex.org/keywords/antibody-neutralization","display_name":"antibody neutralization","score":0.5809999704360962},{"id":"https://openalex.org/keywords/codon-optimization","display_name":"codon optimization","score":0.6669999957084656},{"id":"https://openalex.org/keywords/post-translational-modification-sites","display_name":"post-translational modification sites","score":0.722000002861023},{"id":"https://openalex.org/keywords/poliovirus-serotypes","display_name":"poliovirus serotypes","score":0.7490000128746033},{"id":"https://openalex.org/keywords/structure-guided-engineering","display_name":"structure-guided engineering","score":0.5080000162124634}],"concepts":[{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.8065000176429749},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.6065000295639038},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5590000152587891},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.535099983215332},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.510699987411499},{"id":"https://openalex.org/C14086860","wikidata":"https://www.wikidata.org/wiki/Q337120","display_name":"Neutralization","level":3,"score":0.48840001225471497},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4700999855995178},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43779999017715454},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.43700000643730164},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.43540000915527344},{"id":"https://openalex.org/C2779631682","wikidata":"https://www.wikidata.org/wiki/Q12438936","display_name":"Poliovirus","level":3,"score":0.38420000672340393},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.3589000105857849},{"id":"https://openalex.org/C2776471321","wikidata":"https://www.wikidata.org/wiki/Q7166526","display_name":"Peptide library","level":4,"score":0.35760000348091125},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.35530000925064087},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3215999901294708},{"id":"https://openalex.org/C2779261636","wikidata":"https://www.wikidata.org/wiki/Q7003054","display_name":"Neutralizing antibody","level":3,"score":0.3098999857902527},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.30000001192092896},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.2957000136375427},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.28949999809265137},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2833000123500824},{"id":"https://openalex.org/C36394416","wikidata":"https://www.wikidata.org/wiki/Q949267","display_name":"Immunoglobulin light chain","level":3,"score":0.28200000524520874},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.27090001106262207},{"id":"https://openalex.org/C2777701055","wikidata":"https://www.wikidata.org/wiki/Q1427096","display_name":"Immunotherapy","level":3,"score":0.2655999958515167},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.25920000672340393},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.2513999938964844}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.20.753044","doi":"https://doi.org/10.64898/2026.09.20.753044","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.20.753044","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/22/2026.09.20.753044.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.20.753044","doi":"https://doi.org/10.64898/2026.09.20.753044","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.20.753044","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/22/2026.09.20.753044.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8557374869","display_name":"PATH - to establish an international standard for both manufacturers and regulators of Sabin Inactivated Polio Vaccine as they proceed down the developmen...","funder_award_id":"INV-010160","funder_id":"https://openalex.org/F4320306137","funder_display_name":"Bill and Melinda Gates Foundation"}],"funders":[{"id":"https://openalex.org/F4320306137","display_name":"Bill and Melinda Gates Foundation","ror":"https://ror.org/0456r8d26"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213985207.pdf","grobid_xml":"https://content.openalex.org/works/W7213985207.grobid-xml"},"referenced_works_count":7,"referenced_works":["https://openalex.org/W1939141202","https://openalex.org/W2210358489","https://openalex.org/W2811304917","https://openalex.org/W2991493521","https://openalex.org/W3043678511","https://openalex.org/W3212168392","https://openalex.org/W4412466015"],"related_works":["https://openalex.org/W2603365441","https://openalex.org/W2079277731","https://openalex.org/W7096851214","https://openalex.org/W2123391493","https://openalex.org/W1970207638","https://openalex.org/W2977271446","https://openalex.org/W2069731750","https://openalex.org/W3007607895","https://openalex.org/W2913923466","https://openalex.org/W1812586643"],"abstract_inverted_index":{"Background":[0],"Vaccines":[1],"have":[2],"been":[3],"an":[4,149],"essential":[5],"tool":[6],"in":[7,137,150,160],"bringing":[8],"the":[9,19,98,193],"world":[10],"close":[11],"to":[12,68,123,133],"polio":[13],"eradication,":[14],"with":[15,48],"over":[16],"99.9%":[17],"of":[18,23,38,175,192,199],"global":[20],"population":[21],"free":[22],"poliovirus.":[24,165],"No":[25],"antiviral":[26],"drugs":[27],"or":[28],"monoclonal":[29,45],"antibody":[30,46,195],"products,":[31],"however,":[32],"are":[33],"currently":[34],"licensed":[35],"for":[36,204],"treatment":[37],"polio.":[39],"We":[40],"previously":[41],"isolated":[42],"a":[43,61,112,200,208],"human":[44,113],"(9H2)":[47],"potent":[49],"neutralizing":[50,162],"activity":[51,163],"against":[52,164],"all":[53],"three":[54],"poliovirus":[55,152],"serotypes.":[56],"To":[57],"advance":[58],"9H2":[59,194],"as":[60,207],"therapeutic":[62],"candidate,":[63],"we":[64],"optimized":[65],"its":[66],"sequence":[67,89,196],"extend":[69,124],"serum":[70,125],"half-life":[71],"and":[72,86,109,148,181,187,197],"improve":[73],"manufacturability.":[74],"Methods":[75],"A":[76,166],"Multi-Attribute":[77],"Method":[78],"under":[79],"stress":[80],"conditions":[81],"identified":[82],"post-translational":[83],"modification":[84],"sites,":[85],"Abacus\u2122":[87],"ranked":[88],"liabilities.":[90],"Six":[91],"amino":[92],"acid":[93],"substitutions":[94],"were":[95,107,128,143],"introduced":[96],"into":[97,111,130],"variable":[99],"regions,":[100],"generating":[101],"23":[102],"combinatorial":[103],"variants.":[104],"Codon-optimized":[105],"genes":[106],"synthesized":[108],"engineered":[110],"immunoglobulin":[114],"G1":[115],"backbone":[116],"containing":[117],"crystallizable":[118],"fragment":[119],"(Fc)":[120],"mutations":[121],"(M428L/N434S)":[122],"half-life.":[126],"Constructs":[127],"transfected":[129],"CHO-K1":[131],"cells":[132],"generate":[134],"stable":[135],"pools":[136],"24-well":[138],"plates.":[139],"Protein":[140],"A\u2013purified":[141],"antibodies":[142],"characterized":[144],"using":[145],"biophysical":[146,176],"assays":[147,180],"vitro":[151,161],"neutralization":[153],"assay.":[154],"Results":[155],"All":[156],"variants":[157],"retained":[158],"high":[159],"lead":[167,201],"candidate":[168,202],"was":[169],"selected":[170],"based":[171],"on":[172],"integrated":[173],"assessment":[174],"properties":[177],"across":[178],"eight":[179],"expression":[182],"yield.":[183],"Conclusions":[184],"Structure-guided":[185],"engineering":[186],"experimental":[188],"evaluation":[189],"enabled":[190],"optimization":[191],"identification":[198],"suitable":[203],"clinical":[205],"manufacturing":[206],"potential":[209],"anti-poliovirus":[210],"immunotherapeutic":[211],"agent.":[212]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-09-22T00:00:00"}