{"id":"https://openalex.org/W7213766780","doi":"https://doi.org/10.64898/2026.09.16.752027","title":"Peptidyl Protease Inhibitors Block ERM-BP Function and Suppress Entamoeba Encystation","display_name":"Peptidyl Protease Inhibitors Block ERM-BP Function and Suppress Entamoeba Encystation","publication_year":2026,"publication_date":"2026-09-20","ids":{"openalex":"https://openalex.org/W7213766780","doi":"https://doi.org/10.64898/2026.09.16.752027"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.16.752027","doi":"https://doi.org/10.64898/2026.09.16.752027","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.16.752027","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/20/2026.09.16.752027.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/20/2026.09.16.752027.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061568168","display_name":"Shreyasee Hazra","orcid":"https://orcid.org/0009-0005-3162-5753","observed_orcids":["https://orcid.org/0009-0005-3162-5753"]},"institutions":[{"id":"https://openalex.org/I34034455","display_name":"Ramakrishna Mission Vivekananda Educational and Research Institute","ror":"https://ror.org/03kp2qt98","country_code":"IN","type":"education","lineage":["https://openalex.org/I34034455"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Shreyasee Hazra","raw_affiliation_strings":["Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India"],"raw_orcid":"https://orcid.org/0009-0005-3162-5753","affiliations":[{"raw_affiliation_string":"Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India","institution_ids":["https://openalex.org/I34034455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5092756168","display_name":"Suman Kalyan Dinda","orcid":"https://orcid.org/0009-0005-6345-2535","observed_orcids":["https://orcid.org/0009-0005-6345-2535"]},"institutions":[{"id":"https://openalex.org/I34034455","display_name":"Ramakrishna Mission Vivekananda Educational and Research Institute","ror":"https://ror.org/03kp2qt98","country_code":"IN","type":"education","lineage":["https://openalex.org/I34034455"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Suman Kalyan Dinda","raw_affiliation_strings":["Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India"],"raw_orcid":"https://orcid.org/0009-0005-6345-2535","affiliations":[{"raw_affiliation_string":"Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India","institution_ids":["https://openalex.org/I34034455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136238609","display_name":"Somashree Pandit","orcid":"https://orcid.org/0009-0006-5816-814X","observed_orcids":["https://orcid.org/0009-0006-5816-814X"]},"institutions":[{"id":"https://openalex.org/I34034455","display_name":"Ramakrishna Mission Vivekananda Educational and Research Institute","ror":"https://ror.org/03kp2qt98","country_code":"IN","type":"education","lineage":["https://openalex.org/I34034455"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Somashree Pandit","raw_affiliation_strings":["Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India"],"raw_orcid":"https://orcid.org/0009-0006-5816-814X","affiliations":[{"raw_affiliation_string":"Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India","institution_ids":["https://openalex.org/I34034455"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054469597","display_name":"Dipak Manna","orcid":"https://orcid.org/0000-0001-5112-3957","observed_orcids":["https://orcid.org/0000-0001-5112-3957"]},"institutions":[{"id":"https://openalex.org/I34034455","display_name":"Ramakrishna Mission Vivekananda Educational and Research Institute","ror":"https://ror.org/03kp2qt98","country_code":"IN","type":"education","lineage":["https://openalex.org/I34034455"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Dipak Manna","raw_affiliation_strings":["Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India"],"raw_orcid":"https://orcid.org/0000-0001-5112-3957","affiliations":[{"raw_affiliation_string":"Department of Biomedical Science and Technology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India","institution_ids":["https://openalex.org/I34034455"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5054469597"],"corresponding_institution_ids":["https://openalex.org/I34034455"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12463","display_name":"Amoebic Infections and Treatments","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12463","display_name":"Amoebic Infections and Treatments","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11916","display_name":"Legionella and Acanthamoeba research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transcription-factor-inhibition","display_name":"transcription factor inhibition","score":0.5950000286102295},{"id":"https://openalex.org/keywords/lek","display_name":"Lek","score":0.7120000123977661},{"id":"https://openalex.org/keywords/dna-binding-activity","display_name":"DNA-binding activity","score":0.6869999766349792},{"id":"https://openalex.org/keywords/cyst-morphology","display_name":"cyst morphology","score":0.6890000104904175},{"id":"https://openalex.org/keywords/excystation","display_name":"excystation","score":0.847000002861023},{"id":"https://openalex.org/keywords/trophozoite-growth","display_name":"trophozoite growth","score":0.8009999990463257},{"id":"https://openalex.org/keywords/entamoeba-histolytica","display_name":"Entamoeba histolytica","score":0.8479999899864197}],"concepts":[{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.6553999781608582},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5963000059127808},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5928000211715698},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.5260999798774719},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.5092999935150146},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5012000203132629},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.49570000171661377},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.47769999504089355},{"id":"https://openalex.org/C2776867272","wikidata":"https://www.wikidata.org/wiki/Q131027","display_name":"Entamoeba histolytica","level":2,"score":0.4650999903678894},{"id":"https://openalex.org/C2777473665","wikidata":"https://www.wikidata.org/wiki/Q518891","display_name":"Entamoeba","level":3,"score":0.4618000090122223},{"id":"https://openalex.org/C161624437","wikidata":"https://www.wikidata.org/wiki/Q1988322","display_name":"Small molecule","level":2,"score":0.4586000144481659},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.41819998621940613},{"id":"https://openalex.org/C2781143361","wikidata":"https://www.wikidata.org/wiki/Q55761529","display_name":"Protease inhibitor (pharmacology)","level":5,"score":0.38589999079704285},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.351500004529953},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.3310000002384186},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.3257000148296356},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3156999945640564},{"id":"https://openalex.org/C168831097","wikidata":"https://www.wikidata.org/wiki/Q413248","display_name":"Depsipeptide","level":2,"score":0.31130000948905945},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.30390000343322754},{"id":"https://openalex.org/C116073593","wikidata":"https://www.wikidata.org/wiki/Q864491","display_name":"Biological activity","level":3,"score":0.30090001225471497},{"id":"https://openalex.org/C64903051","wikidata":"https://www.wikidata.org/wiki/Q2198549","display_name":"Drug development","level":3,"score":0.2957000136375427},{"id":"https://openalex.org/C2777283782","wikidata":"https://www.wikidata.org/wiki/Q419343","display_name":"Cysteine protease","level":4,"score":0.29490000009536743},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.29280000925064087},{"id":"https://openalex.org/C154428179","wikidata":"https://www.wikidata.org/wiki/Q24721050","display_name":"PDZ domain","level":2,"score":0.28619998693466187},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.2793000042438507},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.2773999869823456},{"id":"https://openalex.org/C171852809","wikidata":"https://www.wikidata.org/wiki/Q731154","display_name":"Chemical biology","level":2,"score":0.2770000100135803},{"id":"https://openalex.org/C27153228","wikidata":"https://www.wikidata.org/wiki/Q3787274","display_name":"Transcriptional regulation","level":4,"score":0.2651999890804291},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.26089999079704285},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.25270000100135803},{"id":"https://openalex.org/C6555983","wikidata":"https://www.wikidata.org/wiki/Q7181398","display_name":"Phenotypic screening","level":4,"score":0.250900000333786}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.16.752027","doi":"https://doi.org/10.64898/2026.09.16.752027","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.16.752027","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/20/2026.09.16.752027.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.16.752027","doi":"https://doi.org/10.64898/2026.09.16.752027","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.16.752027","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/20/2026.09.16.752027.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8560000061988831,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306227","display_name":"Arthritis National Research Foundation","ror":"https://ror.org/03abbex33"},{"id":"https://openalex.org/F4320320717","display_name":"Department of Biotechnology, Ministry of Science and Technology, India","ror":"https://ror.org/03tjsyq23"},{"id":"https://openalex.org/F4320334771","display_name":"Science and Engineering Research Board","ror":"https://ror.org/03ffdsr55"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213766780.pdf","grobid_xml":"https://content.openalex.org/works/W7213766780.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3043439809","https://openalex.org/W2898991239","https://openalex.org/W2059866515","https://openalex.org/W2128851678","https://openalex.org/W4404345014","https://openalex.org/W133641634","https://openalex.org/W4405848758","https://openalex.org/W2067875953","https://openalex.org/W216948627","https://openalex.org/W4399726969"],"abstract_inverted_index":{"ABSTRACT":[0],"Entamoeba":[1,234],"encystation":[2,188,210],"process":[3,8],"is":[4,97],"a":[5,42,52,69,86,166,237],"crucial":[6],"developmental":[7],"that":[9,78],"support":[10],"to":[11,59,74,125,137,156,199,201,228],"parasite":[12,243],"persistence":[13],"and":[14,28,45,109,152,158,178,183,185,193,223,231,245],"disease":[15,61],"transmission,":[16],"yet":[17],"the":[18,64,81,90,131,135,160,233],"molecular":[19],"mechanisms":[20],"governing":[21],"this":[22,94],"stage":[23],"conversion":[24],"remain":[25],"poorly":[26],"understood":[27],"represent":[29],"an":[30],"attractive":[31],"opportunity":[32],"for":[33,55,239],"therapeutic":[34],"intervention.":[35],"The":[36],"Encystation":[37],"Regulatory":[38],"Motif-Binding":[39],"Protein":[40,117],"(ERM-BP),":[41],"transcription":[43],"factor":[44],"key":[46],"regulator":[47],"of":[48,83,93,163,175],"encystation,":[49,235],"therefore":[50],"represents":[51],"promising":[53,226],"target":[54,229],"anti-amoebic":[56],"drug":[57,71],"development":[58],"prevent":[60],"transmission.":[62],"In":[63],"present":[65],"study,":[66],"we":[67],"applied":[68],"target-based":[70],"discovery":[72],"approach":[73],"screen":[75],"small":[76],"molecules":[77],"can":[79],"block":[80,232],"activity":[82,162,174],"ERM-BP,":[84],"with":[85],"particular":[87],"focus":[88],"on":[89,180,209],"Cys-198":[91],"residue":[92],"protein,":[95],"which":[96,197],"functionally":[98],"important.":[99],"A":[100],"library":[101],"comprising":[102],"100":[103],"compounds":[104,123,139,227],"including":[105],"broad-spectrum":[106],"nicotinamidase":[107],"inhibitors":[108,221],"cysteine-reactive":[110],"electrophiles":[111],"were":[112],"screened":[113],"against":[114],"ERM-BP":[115,127,164,230],"by":[116],"thermal":[118],"shift":[119],"assays":[120],"(PTSA).":[121],"Thirty-six":[122],"bound":[124],"wild-type":[126],"while":[128],"analysis":[129],"using":[130],"C198A":[132],"mutant":[133],"narrowed":[134],"candidates":[136],"three":[138,144],"exhibiting":[140],"Cys-198-dependent":[141],"interactions.":[142],"These":[143],"peptidyl":[145,219],"protease":[146,220],"inhibitors,":[147],"PFMK":[148,184,222],"(Z-Ala-Phe-FMK),":[149],"LEK":[150,177,204],"(Z-Leu-EK),":[151],"EBLL":[153,179,186,224],"(Ethylbenzyl-Leu-Lys)":[154],"binds":[155],"ERM-BP-WT":[157],"abolishes":[159],"DNA-binding":[161],"in":[165],"concentration-dependent":[167],"way.":[168],"Cellular":[169],"evaluation":[170],"further":[171],"revealed":[172],"distinct":[173],"PFMK,":[176],"trophozoite":[181],"growth":[182],"reduced":[187],"efficiency":[189,211],"significantly,":[190],"produced":[191],"irregular,":[192],"structurally":[194],"defective":[195],"cysts,":[196],"fails":[198],"excyst":[200],"trophozoites,":[202],"whereas":[203],"had":[205],"no":[206],"significant":[207],"effects":[208],"or":[212],"cyst":[213],"morphology.":[214],"Altogether,":[215],"these":[216],"findings":[217],"identify":[218],"as":[225],"providing":[236],"framework":[238],"developing":[240],"therapeutics":[241],"targeting":[242],"differentiation":[244],"encystation.":[246]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-09-21T00:00:00"}