{"id":"https://openalex.org/W7213750249","doi":"https://doi.org/10.64898/2026.09.18.752540","title":"Genetic diversity of Legionella species in culture-negative clinical and environmental specimens by sequencing the 23S-5S ribosomal intergenic spacer region","display_name":"Genetic diversity of Legionella species in culture-negative clinical and environmental specimens by sequencing the 23S-5S ribosomal intergenic spacer region","publication_year":2026,"publication_date":"2026-09-19","ids":{"openalex":"https://openalex.org/W7213750249","doi":"https://doi.org/10.64898/2026.09.18.752540"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.18.752540","doi":"https://doi.org/10.64898/2026.09.18.752540","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.18.752540","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/19/2026.09.18.752540.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/19/2026.09.18.752540.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5107339234","display_name":"Camille M. Jacqueline","orcid":"https://orcid.org/0000-0002-2420-7079","observed_orcids":["https://orcid.org/0000-0002-2420-7079","https://orcid.org/0000-0002-6984-4207"]},"institutions":[{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Camille Jacqueline","raw_affiliation_strings":["EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","institution_ids":[]},{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021520097","display_name":"Aur\u00e9lie Peticca","orcid":"https://orcid.org/0000-0002-8972-0973","observed_orcids":["https://orcid.org/0000-0002-8972-0973"]},"institutions":[{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Aur\u00e9lie Peticca","raw_affiliation_strings":["Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5152310732","display_name":"Justine Lannes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Justine Lannes","raw_affiliation_strings":["Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5119430522","display_name":"Matthieu Curtil-Dit-Galin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Matthieu Curtil-Dit-Galin","raw_affiliation_strings":["Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018135756","display_name":"Marine Ibranosyan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Marine Ibranosyan","raw_affiliation_strings":["Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110502518","display_name":"La\u00ebtitia Beraud","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Laetitia B\u00e9raud","raw_affiliation_strings":["Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019575802","display_name":"Ghislaine Descours","orcid":"https://orcid.org/0000-0001-5745-5186","observed_orcids":["https://orcid.org/0000-0001-5745-5186"]},"institutions":[{"id":"https://openalex.org/I100532134","display_name":"Lyon 1 Universit\u00e9","ror":"https://ror.org/029brtt94","country_code":"FR","type":"education","lineage":["https://openalex.org/I100532134","https://openalex.org/I203339264"]},{"id":"https://openalex.org/I113428412","display_name":"\u00c9cole Normale Sup\u00e9rieure de Lyon","ror":"https://ror.org/04zmssz18","country_code":"FR","type":"education","lineage":["https://openalex.org/I113428412","https://openalex.org/I203339264"]},{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]},{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]},{"id":"https://openalex.org/I4210161906","display_name":"Centre International de Recherche en Infectiologie","ror":"https://ror.org/059sz6q14","country_code":"FR","type":"facility","lineage":["https://openalex.org/I100532134","https://openalex.org/I113428412","https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I203339264","https://openalex.org/I4210100596","https://openalex.org/I4210161906","https://openalex.org/I4405259976"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Ghislaine Descours","raw_affiliation_strings":["EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","Universit\u00e9 Lyon 1, INSERM, ENS de Lyon, CNRS, CIRI, UMR 5308, UMR S1111, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","institution_ids":[]},{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]},{"raw_affiliation_string":"Universit\u00e9 Lyon 1, INSERM, ENS de Lyon, CNRS, CIRI, UMR 5308, UMR S1111, Lyon, France","institution_ids":["https://openalex.org/I100532134","https://openalex.org/I113428412","https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I4210161906"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080571985","display_name":"Sophie Jarraud","orcid":"https://orcid.org/0000-0001-5750-0215","observed_orcids":["https://orcid.org/0000-0001-5750-0215"]},"institutions":[{"id":"https://openalex.org/I100532134","display_name":"Lyon 1 Universit\u00e9","ror":"https://ror.org/029brtt94","country_code":"FR","type":"education","lineage":["https://openalex.org/I100532134","https://openalex.org/I203339264"]},{"id":"https://openalex.org/I113428412","display_name":"\u00c9cole Normale Sup\u00e9rieure de Lyon","ror":"https://ror.org/04zmssz18","country_code":"FR","type":"education","lineage":["https://openalex.org/I113428412","https://openalex.org/I203339264"]},{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]},{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]},{"id":"https://openalex.org/I4210161906","display_name":"Centre International de Recherche en Infectiologie","ror":"https://ror.org/059sz6q14","country_code":"FR","type":"facility","lineage":["https://openalex.org/I100532134","https://openalex.org/I113428412","https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I203339264","https://openalex.org/I4210100596","https://openalex.org/I4210161906","https://openalex.org/I4405259976"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Sophie Jarraud","raw_affiliation_strings":["EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","Hospices Civils de Lyon, GenEPII Sequencing Platform Nord, Lyon, France","Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","Universit\u00e9 Lyon 1, INSERM, ENS de Lyon, CNRS, CIRI, UMR 5308, UMR S1111, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","institution_ids":[]},{"raw_affiliation_string":"Hospices Civils de Lyon, GenEPII Sequencing Platform Nord, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]},{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]},{"raw_affiliation_string":"Universit\u00e9 Lyon 1, INSERM, ENS de Lyon, CNRS, CIRI, UMR 5308, UMR S1111, Lyon, France","institution_ids":["https://openalex.org/I100532134","https://openalex.org/I113428412","https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I4210161906"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039199683","display_name":"Christophe Gin\u00e9vra","orcid":"https://orcid.org/0000-0002-5597-094X","observed_orcids":["https://orcid.org/0000-0002-5597-094X"]},"institutions":[{"id":"https://openalex.org/I100532134","display_name":"Lyon 1 Universit\u00e9","ror":"https://ror.org/029brtt94","country_code":"FR","type":"education","lineage":["https://openalex.org/I100532134","https://openalex.org/I203339264"]},{"id":"https://openalex.org/I113428412","display_name":"\u00c9cole Normale Sup\u00e9rieure de Lyon","ror":"https://ror.org/04zmssz18","country_code":"FR","type":"education","lineage":["https://openalex.org/I113428412","https://openalex.org/I203339264"]},{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]},{"id":"https://openalex.org/I4210100596","display_name":"Hospices Civils de Lyon","ror":"https://ror.org/01502ca60","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I4210100596"]},{"id":"https://openalex.org/I4210161906","display_name":"Centre International de Recherche en Infectiologie","ror":"https://ror.org/059sz6q14","country_code":"FR","type":"facility","lineage":["https://openalex.org/I100532134","https://openalex.org/I113428412","https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I203339264","https://openalex.org/I4210100596","https://openalex.org/I4210161906","https://openalex.org/I4405259976"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Christophe Ginevra","raw_affiliation_strings":["EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","Hospices Civils de Lyon, GenEPII Sequencing Platform Nord, Lyon, France","Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","Universit\u00e9 Lyon 1, INSERM, ENS de Lyon, CNRS, CIRI, UMR 5308, UMR S1111, Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"EU Reference Laboratory for Public Health on Legionella (EURL-PH-LEGI)","institution_ids":[]},{"raw_affiliation_string":"Hospices Civils de Lyon, GenEPII Sequencing Platform Nord, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]},{"raw_affiliation_string":"Hospices Civils de Lyon, Institut des Agents infectieux, Centre National de r\u00e9f\u00e9rence des L\u00e9gionelles, Lyon, France","institution_ids":["https://openalex.org/I4210100596"]},{"raw_affiliation_string":"Universit\u00e9 Lyon 1, INSERM, ENS de Lyon, CNRS, CIRI, UMR 5308, UMR S1111, Lyon, France","institution_ids":["https://openalex.org/I100532134","https://openalex.org/I113428412","https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I4210161906"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5107339234"],"corresponding_institution_ids":["https://openalex.org/I4210100596"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11916","display_name":"Legionella and Acanthamoeba research","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11916","display_name":"Legionella and Acanthamoeba research","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12824","display_name":"Infections and bacterial resistance","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/legionella","display_name":"Legionella","score":0.902999997138977},{"id":"https://openalex.org/keywords/legionnaires-disease","display_name":"Legionnaires' disease","score":0.9300000071525574},{"id":"https://openalex.org/keywords/species-identification","display_name":"species identification","score":0.8180000185966492},{"id":"https://openalex.org/keywords/genetic-diversity","display_name":"genetic diversity","score":0.6110000014305115},{"id":"https://openalex.org/keywords/coinfection","display_name":"co-infection","score":0.8519999980926514},{"id":"https://openalex.org/keywords/legionella-longbeachae","display_name":"Legionella longbeachae","score":0.8830000162124634},{"id":"https://openalex.org/keywords/legionella-bozemanii","display_name":"Legionella bozemanii","score":0.9959999918937683},{"id":"https://openalex.org/keywords/environmental-water-samples","display_name":"environmental water samples","score":0.5329999923706055},{"id":"https://openalex.org/keywords/bioinformatics-pipeline","display_name":"bioinformatics pipeline","score":0.7829999923706055},{"id":"https://openalex.org/keywords/reference-database","display_name":"reference database","score":0.7559999823570251},{"id":"https://openalex.org/keywords/outbreak-investigation","display_name":"outbreak investigation","score":0.7870000004768372},{"id":"https://openalex.org/keywords/molecular-surveillance","display_name":"molecular surveillance","score":0.8820000290870667}],"concepts":[{"id":"https://openalex.org/C2779164008","wikidata":"https://www.wikidata.org/wiki/Q158013","display_name":"Legionella","level":3,"score":0.8787999749183655},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7271999716758728},{"id":"https://openalex.org/C81566733","wikidata":"https://www.wikidata.org/wiki/Q1047544","display_name":"Hypervariable region","level":3,"score":0.6751000285148621},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.6656000018119812},{"id":"https://openalex.org/C2776118409","wikidata":"https://www.wikidata.org/wiki/Q147885","display_name":"Legionella pneumophila","level":3,"score":0.4941999912261963},{"id":"https://openalex.org/C195139083","wikidata":"https://www.wikidata.org/wiki/Q3463539","display_name":"Intergenic region","level":4,"score":0.48820000886917114},{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.4871000051498413},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.477400004863739},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.46799999475479126},{"id":"https://openalex.org/C2780295090","wikidata":"https://www.wikidata.org/wiki/Q154882","display_name":"Legionnaires' disease","level":4,"score":0.4652000069618225},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4602999985218048},{"id":"https://openalex.org/C97669555","wikidata":"https://www.wikidata.org/wiki/Q4118435","display_name":"Molecular epidemiology","level":4,"score":0.43959999084472656},{"id":"https://openalex.org/C116675565","wikidata":"https://www.wikidata.org/wiki/Q3241045","display_name":"Outbreak","level":2,"score":0.43320000171661377},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.40290001034736633},{"id":"https://openalex.org/C81977670","wikidata":"https://www.wikidata.org/wiki/Q585259","display_name":"Genetic diversity","level":3,"score":0.3571999967098236},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.35100001096725464},{"id":"https://openalex.org/C22860579","wikidata":"https://www.wikidata.org/wiki/Q7322402","display_name":"Ribosomal Intergenic Spacer analysis","level":5,"score":0.34769999980926514},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.3154999911785126},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.30160000920295715},{"id":"https://openalex.org/C207812311","wikidata":"https://www.wikidata.org/wiki/Q426560","display_name":"Ribosomal DNA","level":4,"score":0.2962000072002411},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.2822999954223633},{"id":"https://openalex.org/C24432333","wikidata":"https://www.wikidata.org/wiki/Q2068526","display_name":"Whole genome sequencing","level":4,"score":0.27410000562667847},{"id":"https://openalex.org/C90132467","wikidata":"https://www.wikidata.org/wiki/Q171184","display_name":"Phylogenetics","level":3,"score":0.2599000036716461},{"id":"https://openalex.org/C190789776","wikidata":"https://www.wikidata.org/wiki/Q767511","display_name":"Genetic marker","level":3,"score":0.257099986076355}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.18.752540","doi":"https://doi.org/10.64898/2026.09.18.752540","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.18.752540","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/19/2026.09.18.752540.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.18.752540","doi":"https://doi.org/10.64898/2026.09.18.752540","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.18.752540","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/19/2026.09.18.752540.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213750249.pdf","grobid_xml":"https://content.openalex.org/works/W7213750249.grobid-xml"},"referenced_works_count":13,"referenced_works":["https://openalex.org/W1552537924","https://openalex.org/W1937531655","https://openalex.org/W2021878136","https://openalex.org/W2064580110","https://openalex.org/W2142678478","https://openalex.org/W2237413009","https://openalex.org/W2401404581","https://openalex.org/W2591872324","https://openalex.org/W2807194798","https://openalex.org/W2963276645","https://openalex.org/W2987197248","https://openalex.org/W3152617629","https://openalex.org/W4284894346"],"related_works":["https://openalex.org/W1552537924","https://openalex.org/W2233154463","https://openalex.org/W2003862061","https://openalex.org/W2064580110","https://openalex.org/W1556882782","https://openalex.org/W4389119941","https://openalex.org/W2496568986","https://openalex.org/W2148490451","https://openalex.org/W4406952560","https://openalex.org/W2321742949"],"abstract_inverted_index":{"Abstract":[0],"The":[1,180],"diagnosis":[2],"of":[3,20,41,80,123,139,171,176,229,322,330,353,369],"Legionnaires\u2019":[4,234],"disease":[5,42,235],"(LD)":[6],"caused":[7,236],"by":[8,188,237],"Legionella":[9,23,77,115,178,240,264,293,331],"non-":[10,238],"pneumophila":[11,141,239],"species":[12,29,78,116,241,245],"is":[13,36,247],"likely":[14],"to":[15,38,45,105,113,154,286],"increase":[16],"with":[17,59,70,142,225,268],"broader":[18],"use":[19],"PCR":[21],"targeting":[22,262],"spp.":[24],"In":[25],"this":[26,230,341,354,370],"context,":[27],"accurate":[28],"identification":[30,167,246,280],"in":[31,120,134,168,184,295,319,340,345,349,361,365],"PCR-positive":[32,108,283],"but":[33],"culture-negative":[34,107],"samples":[35,109,125,151,297],"essential":[37],"improve":[39],"understanding":[40,329],"epidemiology":[43],"and":[44,54,88,93,96,126,186,201,256,273,288,298,310,316],"support":[46],"source":[47],"attribution.":[48],"Here,":[49],"we":[50],"presented":[51],"a":[52,71,128,189,194,258,269,274],"validated":[53,257],"user-friendly":[55,275],"bioinformatic":[56,276],"pipeline":[57,103],"compatible":[58],"next-generation":[60,259],"sequencing":[61,260],"(NGS)":[62],"for":[63,86,197],"analyzing":[64],"the":[65,102,169,213,226,263,320,350,366],"hypervariable":[66],"23S-5S":[67,162,265],"region,":[68,266],"paired":[69],"curated":[72,270],"database":[73,272],"encompassing":[74],"all":[75],"described":[76],"as":[79,325,327],"January":[81],"2026.":[82],"Parameters":[83],"were":[84,117,132],"optimized":[85],"sensitivity":[87],"specificity":[89],"using":[90],"both":[91],"strains":[92],"culture-positive":[94],"clinical":[95,135,287],"environmental":[97,150,289,296],"samples.":[98,284],"We":[99,254],"then":[100],"applied":[101],"retrospectively":[104],"92":[106],"collected":[110],"from":[111,282],"2023":[112],"2025.":[114],"successfully":[118],"assigned":[119],"60%":[121],"(55/92)":[122],"tested":[124],"revealed":[127,291],"high":[129],"diversity.":[130,332],"Co-infections":[131],"detected":[133,299],"samples,":[136],"including":[137],"combinations":[138],"L.":[140,143,146],"longbeachae":[144],"or":[145,215],"bozemanii":[147],",":[148],"while":[149],"contained":[152],"up":[153],"six":[155],"different":[156],"species.":[157],"These":[158],"results":[159],"demonstrate":[160],"that":[161,302],"amplicon":[163],"NGS":[164],"enables":[165],"species-level":[166,279],"absence":[170],"cultured":[172],"isolates,":[173],"improving":[174],"surveillance":[175,315],"non-pneumophila":[177],"cases.":[179],"proposed":[181],"pipeline,":[182,277],"implemented":[183],"QIIME2":[185],"accompanied":[187],"publicly":[190],"available":[191,224,344,348,364],"database,":[192],"provides":[193],"practical":[195],"framework":[196,312],"routine":[198],"molecular":[199,314],"monitoring":[200],"outbreak":[202,317],"investigation.":[203],"Author":[204],"Notes":[205],"All":[206,335],"supporting":[207],"data":[208,218],"have":[209,305],"been":[210,306],"provided":[211],"within":[212],"article":[214],"through":[216],"supplementary":[217,221],"files.":[219],"Four":[220],"tables":[222],"are":[223,252,343],"online":[227,351,367],"version":[228,352,368],"article.":[231,355,371],"Impact":[232],"statement":[233],"remains":[242],"under-characterized":[243],"because":[244],"often":[248],"impossible":[249],"when":[250],"cultures":[251],"negative.":[253],"developed":[255],"approach":[261],"coupled":[267],"reference":[271],"enabling":[278],"directly":[281],"Application":[285],"specimens":[290],"substantial":[292],"diversity":[294],"mixed-species":[300],"infections":[301],"would":[303],"otherwise":[304],"missed.":[307],"This":[308],"open":[309],"reproducible":[311],"strengthens":[313],"investigations":[318],"era":[321],"culture-independent":[323],"diagnostics,":[324],"well":[326],"our":[328],"Data":[333],"summary":[334],"isolates":[336],"whole-genome":[337],"sequences":[338],"used":[339],"work":[342],"Table":[346,362],"S1,":[347],"Amplicon-specific":[356],"information":[357],"can":[358],"be":[359],"found":[360],"S2,":[363]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-21T00:00:00"}