{"id":"https://openalex.org/W7213538077","doi":"https://doi.org/10.64898/2026.09.16.749688","title":"Three-dimensional imaging reveals distinct muscle-vessel-nerve relationships during dorsal and ventral muscle splitting in the chicken forelimb","display_name":"Three-dimensional imaging reveals distinct muscle-vessel-nerve relationships during dorsal and ventral muscle splitting in the chicken forelimb","publication_year":2026,"publication_date":"2026-09-18","ids":{"openalex":"https://openalex.org/W7213538077","doi":"https://doi.org/10.64898/2026.09.16.749688"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.16.749688","doi":"https://doi.org/10.64898/2026.09.16.749688","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.16.749688","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/18/2026.09.16.749688.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/18/2026.09.16.749688.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112007362","display_name":"Yuta Takase","orcid":"https://orcid.org/0009-0007-8917-6721","observed_orcids":["https://orcid.org/0009-0007-8917-6721"]},"institutions":[{"id":"https://openalex.org/I4210130388","display_name":"Suntory Foundation for Life Sciences","ror":"https://ror.org/02pkrz957","country_code":"JP","type":"nonprofit","lineage":["https://openalex.org/I4210130388"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuta Takase","raw_affiliation_strings":["Bioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, Japan"],"raw_orcid":"https://orcid.org/0009-0007-8917-6721","affiliations":[{"raw_affiliation_string":"Bioorganic Research Institute, Suntory Foundation for Life Sciences, Kyoto, Japan","institution_ids":["https://openalex.org/I4210130388"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085051628","display_name":"Sayaka Tojima","orcid":"https://orcid.org/0000-0002-5886-6056","observed_orcids":["https://orcid.org/0000-0002-5886-6056"]},"institutions":[{"id":"https://openalex.org/I27429435","display_name":"Kyoto Institute of Technology","ror":"https://ror.org/00965ax52","country_code":"JP","type":"education","lineage":["https://openalex.org/I27429435"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Sayaka Tojima","raw_affiliation_strings":["Applied Biology, Kyoto Institute of Technology, Kyoto, Japan"],"raw_orcid":"https://orcid.org/0000-0002-5886-6056","affiliations":[{"raw_affiliation_string":"Applied Biology, Kyoto Institute of Technology, Kyoto, Japan","institution_ids":["https://openalex.org/I27429435"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5085051628"],"corresponding_institution_ids":["https://openalex.org/I27429435"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.8003000020980835,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.8003000020980835,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.12610000371932983,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.009600000455975533,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/embryonic-limb-development","display_name":"embryonic limb development","score":0.8370000123977661},{"id":"https://openalex.org/keywords/tissue-clearing","display_name":"tissue clearing","score":0.7860000133514404},{"id":"https://openalex.org/keywords/cubic","display_name":"CUBIC","score":0.8270000219345093},{"id":"https://openalex.org/keywords/scale","display_name":"scale","score":0.9409999847412109},{"id":"https://openalex.org/keywords/3d-imaging","display_name":"3D imaging","score":0.640999972820282}],"concepts":[{"id":"https://openalex.org/C2778646069","wikidata":"https://www.wikidata.org/wiki/Q5468632","display_name":"Forelimb","level":2,"score":0.8273000121116638},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.796999990940094},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6140999794006348},{"id":"https://openalex.org/C140530291","wikidata":"https://www.wikidata.org/wiki/Q1225063","display_name":"Dorsum","level":2,"score":0.5656999945640564},{"id":"https://openalex.org/C2778514504","wikidata":"https://www.wikidata.org/wiki/Q797559","display_name":"Muscle tissue","level":2,"score":0.4625999927520752},{"id":"https://openalex.org/C51738704","wikidata":"https://www.wikidata.org/wiki/Q5766112","display_name":"Hindlimb","level":2,"score":0.41620001196861267},{"id":"https://openalex.org/C2779395532","wikidata":"https://www.wikidata.org/wiki/Q7916469","display_name":"Vascular smooth muscle","level":3,"score":0.3361999988555908},{"id":"https://openalex.org/C2778961482","wikidata":"https://www.wikidata.org/wiki/Q25241","display_name":"Vertebrate","level":3,"score":0.3361000120639801},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.3264000117778778},{"id":"https://openalex.org/C2908754555","wikidata":"https://www.wikidata.org/wiki/Q2914458","display_name":"Tissue fluid","level":2,"score":0.32010000944137573},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.295199990272522},{"id":"https://openalex.org/C2777663904","wikidata":"https://www.wikidata.org/wiki/Q988343","display_name":"Blood vessel","level":2,"score":0.29089999198913574},{"id":"https://openalex.org/C46762472","wikidata":"https://www.wikidata.org/wiki/Q178648","display_name":"Peripheral","level":2,"score":0.28459998965263367},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.2824000120162964},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.2624000012874603},{"id":"https://openalex.org/C86370654","wikidata":"https://www.wikidata.org/wiki/Q2277862","display_name":"Limb development","level":3,"score":0.2540000081062317}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.16.749688","doi":"https://doi.org/10.64898/2026.09.16.749688","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.16.749688","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/18/2026.09.16.749688.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.16.749688","doi":"https://doi.org/10.64898/2026.09.16.749688","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.16.749688","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/18/2026.09.16.749688.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213538077.pdf","grobid_xml":"https://content.openalex.org/works/W7213538077.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W1522132696","https://openalex.org/W1967399157","https://openalex.org/W1973968540","https://openalex.org/W2075221395","https://openalex.org/W2086206178","https://openalex.org/W2140034535","https://openalex.org/W2153584531","https://openalex.org/W2160763165","https://openalex.org/W2203529929","https://openalex.org/W2208442878","https://openalex.org/W2601841882","https://openalex.org/W2795836428","https://openalex.org/W2897431175","https://openalex.org/W2985403462","https://openalex.org/W2998583117","https://openalex.org/W3087402308","https://openalex.org/W4221025890"],"related_works":["https://openalex.org/W2160763165","https://openalex.org/W2006265390","https://openalex.org/W2013213724","https://openalex.org/W2432165468","https://openalex.org/W2005599982","https://openalex.org/W3116298508","https://openalex.org/W7057035007","https://openalex.org/W2027293024","https://openalex.org/W1973968540","https://openalex.org/W4200005427"],"abstract_inverted_index":{"ABSTRACT":[0],"During":[1],"vertebrate":[2],"limb":[3,200],"development,":[4],"muscles,":[5],"blood":[6,40,118],"vessels,":[7],"and":[8,22,33,79,92,100,120,127,184,203,229],"nerves":[9,231],"undergo":[10],"coordinated":[11,224],"morphogenesis.":[12],"Limb":[13],"muscles":[14],"form":[15],"through":[16],"progressive":[17],"splitting":[18,32,146,160,169,202,207],"of":[19,162,199,226],"the":[20,27,135,145,159,163,171,182,223,233],"dorsal":[21,164,183],"ventral":[23,136,185],"muscle":[24,31,46,55,137,165,186,201],"masses,":[25],"but":[26],"three-dimensional":[28,63,216],"relationships":[29,129],"between":[30,181],"surrounding":[34],"tissues":[35],"remain":[36],"incompletely":[37],"understood.":[38],"Although":[39],"vessels":[41],"have":[42],"been":[43],"implicated":[44],"in":[45,232],"splitting,":[47],"whether":[48],"this":[49],"association":[50],"is":[51,57,194],"shared":[52],"among":[53],"different":[54],"masses":[56],"unclear.":[58],"Here,":[59],"we":[60],"established":[61],"a":[62,113,196],"imaging":[64],"approach":[65,217],"for":[66,94],"chicken":[67],"embryonic":[68,234],"forelimbs":[69],"by":[70],"combining":[71],"fluorescent":[72,97],"vascular":[73,102,108,140,154,192],"labeling,":[74],"whole-mount":[75],"immunostaining,":[76],"tissue":[77,213],"clearing,":[78],"confocal":[80],"microscopy.":[81],"We":[82,110],"compared":[83],"three":[84,107],"hydrophilic":[85],"clearing":[86],"methods,":[87],"Sca":[88],"l":[89],"eS,":[90],"CUBIC,":[91],"SeeDB2,":[93],"compatibility":[95],"with":[96],"ink,":[98],"LCA-lectin,":[99],"PKH26":[101],"labeling.":[103],"SeeDB2":[104,115],"preserved":[105],"all":[106],"signals.":[109],"then":[111],"developed":[112],"modified":[114],"protocol":[116],"incorporating":[117],"decolorization":[119],"used":[121],"it":[122],"to":[123,132],"examine":[124],"muscle,":[125,227],"vessel,":[126],"nerve":[128],"from":[130],"E5.0":[131],"E6.0.":[133],"In":[134,150],"mass,":[138],"multiple":[139],"extensions":[141],"were":[142],"observed":[143],"around":[144,158],"region":[147,161],"at":[148,170],"E5.5.":[149],"contrast,":[151],"no":[152],"prominent":[153],"extension":[155,193],"was":[156],"detected":[157],"mass":[166],"despite":[167],"ongoing":[168],"same":[172],"stage.":[173],"Nerve":[174],"projections":[175],"also":[176],"showed":[177],"distinct":[178,211],"spatial":[179],"patterns":[180],"masses.":[187],"These":[188],"findings":[189],"indicate":[190],"that":[191,205],"not":[195],"universal":[197],"feature":[198],"suggest":[204],"individual":[206],"events":[208],"occur":[209],"within":[210],"local":[212],"environments.":[214],"Our":[215],"provides":[218],"new":[219],"morphological":[220],"insight":[221],"into":[222],"development":[225],"vasculature,":[228],"peripheral":[230],"limb.":[235]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-09-18T00:00:00"}