{"id":"https://openalex.org/W7213464424","doi":"https://doi.org/10.64898/2026.09.15.750731","title":"Evidence for early kidney vasculature development by vasculogenesis and angiogenesis based on advanced in vivo chimeric model and depletion of CD31+/CD146+ intrinsic endothelial progenitors","display_name":"Evidence for early kidney vasculature development by vasculogenesis and angiogenesis based on advanced in vivo chimeric model and depletion of CD31+/CD146+ intrinsic endothelial progenitors","publication_year":2026,"publication_date":"2026-09-17","ids":{"openalex":"https://openalex.org/W7213464424","doi":"https://doi.org/10.64898/2026.09.15.750731"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.15.750731","doi":"https://doi.org/10.64898/2026.09.15.750731","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.15.750731","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.15.750731.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.15.750731.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012756545","display_name":"Susanna Kaisto","orcid":"https://orcid.org/0000-0002-0484-1670","observed_orcids":["https://orcid.org/0000-0002-0484-1670"]},"institutions":[{"id":"https://openalex.org/I175532246","display_name":"University of Eastern Finland","ror":"https://ror.org/00cyydd11","country_code":"FI","type":"education","lineage":["https://openalex.org/I175532246"]},{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Susanna Kaisto","raw_affiliation_strings":["GeneCellNano, University of Eastern Finland, Kuopio, Finland","Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GeneCellNano, University of Eastern Finland, Kuopio, Finland","institution_ids":["https://openalex.org/I175532246"]},{"raw_affiliation_string":"Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085464563","display_name":"Samar Ahmad","orcid":"https://orcid.org/0000-0001-5015-6431","observed_orcids":["https://orcid.org/0000-0001-5015-6431"]},"institutions":[{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Samar Ahmad","raw_affiliation_strings":["Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland"],"raw_orcid":"https://orcid.org/0000-0001-5015-6431","affiliations":[{"raw_affiliation_string":"Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151940933","display_name":"Sabrina Halmbacher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I196349391","display_name":"Universit\u00e4t Ulm","ror":"https://ror.org/032000t02","country_code":"DE","type":"education","lineage":["https://openalex.org/I196349391"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sabrina Halmbacher","raw_affiliation_strings":["Institute of Biochemistry and Molecular Biology, Ulm University, Ulm, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry and Molecular Biology, Ulm University, Ulm, Germany","institution_ids":["https://openalex.org/I196349391"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054337341","display_name":"Virpi Glumoff","orcid":"https://orcid.org/0000-0003-3461-6471","observed_orcids":["https://orcid.org/0000-0003-3461-6471"]},"institutions":[{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Virpi Glumoff","raw_affiliation_strings":["Research Unit of Biomedicine, University of Oulu, Oulu, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Unit of Biomedicine, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086855132","display_name":"Laura D\u00f6nges","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Laura D\u00f6nges","raw_affiliation_strings":["Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053420508","display_name":"Irina B. Raykhel","orcid":"https://orcid.org/0000-0001-7473-7011","observed_orcids":["https://orcid.org/0000-0001-7473-7011"]},"institutions":[{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Irina Raykhel","raw_affiliation_strings":["The Unit of Measurement Technology (MITY), University of Oulu, Oulu, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Unit of Measurement Technology (MITY), University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136257486","display_name":"Ilya Skovorodkin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Ilya Skovorodkin","raw_affiliation_strings":["Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5044475923","display_name":"Seppo Juhani Vainio","orcid":"https://orcid.org/0000-0001-9319-3566","observed_orcids":["https://orcid.org/0000-0001-9319-3566"]},"institutions":[{"id":"https://openalex.org/I4405273007","display_name":"Infotech Oulu","ror":"https://ror.org/0548kxt77","country_code":"FI","type":"facility","lineage":["https://openalex.org/I4405273007"]},{"id":"https://openalex.org/I98381234","display_name":"University of Oulu","ror":"https://ror.org/03yj89h83","country_code":"FI","type":"education","lineage":["https://openalex.org/I98381234"]}],"countries":["FI"],"is_corresponding":true,"raw_author_name":"Seppo J Vainio","raw_affiliation_strings":["Infotech Oulu, University of Oulu, Oulu, Finland","Kvantum Institute, University of Oulu, Oulu, Finland","Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Infotech Oulu, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I4405273007","https://openalex.org/I98381234"]},{"raw_affiliation_string":"Kvantum Institute, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]},{"raw_affiliation_string":"Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland","institution_ids":["https://openalex.org/I98381234"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5044475923"],"corresponding_institution_ids":["https://openalex.org/I4405273007","https://openalex.org/I98381234"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12140","display_name":"Renal and related cancers","score":0.9375,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12140","display_name":"Renal and related cancers","score":0.9375,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.03970000147819519,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.0044999998062849045,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vasculogenesis","display_name":"vasculogenesis","score":0.9319999814033508},{"id":"https://openalex.org/keywords/angiogenesis","display_name":"angiogenesis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/kidney-organoids","display_name":"kidney organoids","score":0.8610000014305115},{"id":"https://openalex.org/keywords/endothelial-progenitor-cells","display_name":"endothelial progenitor cells","score":0.7269999980926514},{"id":"https://openalex.org/keywords/cd31-endothelial-cells","display_name":"CD31+ endothelial cells","score":0.8889999985694885},{"id":"https://openalex.org/keywords/chorioallantoic-membrane","display_name":"chorioallantoic membrane","score":0.7429999709129333},{"id":"https://openalex.org/keywords/kidney-organogenesis","display_name":"kidney organogenesis","score":0.5450000166893005},{"id":"https://openalex.org/keywords/lineage-tracing","display_name":"lineage tracing","score":0.6259999871253967},{"id":"https://openalex.org/keywords/cell-fate-tracing","display_name":"cell fate tracing","score":0.5860000252723694}],"concepts":[{"id":"https://openalex.org/C2780091579","wikidata":"https://www.wikidata.org/wiki/Q9377","display_name":"Kidney","level":2,"score":0.7017999887466431},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6941999793052673},{"id":"https://openalex.org/C60781801","wikidata":"https://www.wikidata.org/wiki/Q1424593","display_name":"Vasculogenesis","level":4,"score":0.6481000185012817},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6308000087738037},{"id":"https://openalex.org/C2780394083","wikidata":"https://www.wikidata.org/wiki/Q539568","display_name":"Angiogenesis","level":2,"score":0.6225000023841858},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.605400025844574},{"id":"https://openalex.org/C138798377","wikidata":"https://www.wikidata.org/wiki/Q7312502","display_name":"Renal stem cell","level":4,"score":0.513700008392334},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.5044000148773193},{"id":"https://openalex.org/C31695470","wikidata":"https://www.wikidata.org/wiki/Q11293125","display_name":"Organoid","level":2,"score":0.44359999895095825},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.44209998846054077},{"id":"https://openalex.org/C2777632694","wikidata":"https://www.wikidata.org/wiki/Q2336077","display_name":"Kidney development","level":4,"score":0.4041000008583069},{"id":"https://openalex.org/C15729860","wikidata":"https://www.wikidata.org/wiki/Q2329919","display_name":"Progenitor","level":4,"score":0.3614000082015991},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.3580999970436096},{"id":"https://openalex.org/C2781231759","wikidata":"https://www.wikidata.org/wiki/Q58184","display_name":"Nephron","level":3,"score":0.34119999408721924},{"id":"https://openalex.org/C2778653478","wikidata":"https://www.wikidata.org/wiki/Q1054718","display_name":"Kidney disease","level":2,"score":0.2896000146865845},{"id":"https://openalex.org/C171056886","wikidata":"https://www.wikidata.org/wiki/Q193119","display_name":"Regeneration (biology)","level":2,"score":0.2815999984741211},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.2775000035762787},{"id":"https://openalex.org/C2779207114","wikidata":"https://www.wikidata.org/wiki/Q1364012","display_name":"Allantois","level":3,"score":0.27469998598098755},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.273499995470047},{"id":"https://openalex.org/C160351604","wikidata":"https://www.wikidata.org/wiki/Q6033113","display_name":"Ingression","level":5,"score":0.26159998774528503}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.15.750731","doi":"https://doi.org/10.64898/2026.09.15.750731","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.15.750731","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.15.750731.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.15.750731","doi":"https://doi.org/10.64898/2026.09.15.750731","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.15.750731","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.15.750731.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311793","display_name":"Victoriastiftelsen","ror":"https://ror.org/010cn0k09"},{"id":"https://openalex.org/F4320324148","display_name":"Orionin Tutkimuss\u00e4\u00e4ti\u00f6","ror":"https://ror.org/0296s4x19"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213464424.pdf","grobid_xml":"https://content.openalex.org/works/W7213464424.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2339949729","https://openalex.org/W3161158838","https://openalex.org/W4292318605","https://openalex.org/W7133671083","https://openalex.org/W2802577485","https://openalex.org/W4397048410","https://openalex.org/W4398182281","https://openalex.org/W2460039205","https://openalex.org/W2913348902","https://openalex.org/W4226182074"],"abstract_inverted_index":{"ABSTRACT":[0],"Kidney":[1],"diseases":[2],"are":[3],"a":[4,10,42,51,87,131],"significant":[5,33],"health":[6],"concern":[7],"worldwide,":[8],"with":[9,96,168],"growing":[11],"demand":[12],"for":[13],"fundamental":[14],"treatments,":[15],"such":[16],"as":[17],"generation":[18,26],"of":[19,27,67,119,140,147,181],"transplantable":[20],"organs":[21],"from":[22],"programmed":[23],"cells.":[24],"The":[25,92,162],"kidney":[28,48,69,78,111,134,149,186,191],"organoids":[29,49],"has":[30],"led":[31],"to":[32,40,53,109,159],"advances":[34],"in":[35,47,59,184],"disease":[36],"modeling.":[37],"However,":[38],"failure":[39],"establish":[41],"robust":[43],"endothelial":[44,122,176],"vasculature":[45,70],"network":[46],"remains":[50],"barrier":[52],"oxygen":[54],"support":[55],"and":[56,81,117,125,154,165,179],"gas":[57],"exchange":[58],"renal":[60],"tissue":[61],"engineering.":[62],"We":[63,113],"targeted":[64],"the":[65,74,97,115,120,148,157,174,185,190,194,200,207],"development":[66],"early":[68],"system":[71,84],"by":[72,85,129,145,152,206],"advancing":[73],"classic":[75],"mouse":[76,201],"embryonic":[77,133],"organ":[79,150],"culture":[80],"organoid":[82],"model":[83],"introducing":[86],"novel":[88],"mini":[89],"reservoir":[90],"setup.":[91],"method":[93],"improved":[94],"integration":[95],"highly":[98],"vascularized":[99],"chicken":[100],"Chorion":[101],"Allantois":[102],"Membrane":[103],"(CAM),":[104],"enabling":[105],"host":[106],"blood":[107],"flow":[108],"developing":[110],"organoids.":[112],"examined":[114],"origin":[116],"role":[118],"CD31+":[121,182],"cells":[123,171],"(ECs)":[124],"their":[126],"CD146+":[127,177],"progenitors":[128,197],"applying":[130],"dissociated":[132],"approach.":[135],"This":[136],"enabled":[137],"selected":[138],"depletion":[139],"specific":[141],"cell":[142,163],"populations,":[143],"followed":[144],"regeneration":[146],"primordia":[151],"reaggregation":[153],"placement":[155],"on":[156],"CAM":[158,208],"assess":[160],"organogenesis.":[161],"lineage":[164],"fate":[166],"tracing":[167],"constitutively":[169],"labelled":[170],"revealed":[172],"that":[173],"intrinsic":[175,195],"progenitor":[178],"those":[180],"ECs":[183],"initiating":[187],"organogenesis":[188],"established":[189],"vasculature.":[192],"If":[193],"EC":[196],"were":[198],"depleted,":[199],"glomerular":[202],"vascularization":[203],"was":[204],"rescued":[205],"derived":[209],"ECs.":[210]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-09-17T00:00:00"}