{"id":"https://openalex.org/W7213459208","doi":"https://doi.org/10.64898/2026.09.14.751494","title":"Universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids","display_name":"Universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids","publication_year":2026,"publication_date":"2026-09-17","ids":{"openalex":"https://openalex.org/W7213459208","doi":"https://doi.org/10.64898/2026.09.14.751494"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.14.751494","doi":"https://doi.org/10.64898/2026.09.14.751494","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.14.751494","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.14.751494.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.14.751494.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051685249","display_name":"Sebastian Pohl","orcid":"https://orcid.org/0000-0002-4294-9498","observed_orcids":["https://orcid.org/0000-0002-4294-9498"]},"institutions":[{"id":"https://openalex.org/I4210158898","display_name":"Institute of Genetics and Cancer","ror":"https://ror.org/05hygey35","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210087105","https://openalex.org/I4210158898","https://openalex.org/I90344618","https://openalex.org/I98677209"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sebastian \u00d6ther-Gee Pohl","raw_affiliation_strings":["Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056280532","display_name":"Brianda Areli Hern\u00e1ndez-Mor\u00e1n","orcid":"https://orcid.org/0000-0001-5405-0757","observed_orcids":["https://orcid.org/0000-0001-5405-0757"]},"institutions":[{"id":"https://openalex.org/I4210158898","display_name":"Institute of Genetics and Cancer","ror":"https://ror.org/05hygey35","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210087105","https://openalex.org/I4210158898","https://openalex.org/I90344618","https://openalex.org/I98677209"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Brianda A Hern\u00e1ndez Mor\u00e1n","raw_affiliation_strings":["Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151989914","display_name":"Camila Guzman Cardoso","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Camila Guzman Cardozo","raw_affiliation_strings":["Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I98677209"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063348974","display_name":"Gillian C.A. Taylor","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210158898","display_name":"Institute of Genetics and Cancer","ror":"https://ror.org/05hygey35","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210087105","https://openalex.org/I4210158898","https://openalex.org/I90344618","https://openalex.org/I98677209"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Gillian CA Taylor","raw_affiliation_strings":["Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010518369","display_name":"Abram Giller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210158898","display_name":"Institute of Genetics and Cancer","ror":"https://ror.org/05hygey35","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210087105","https://openalex.org/I4210158898","https://openalex.org/I90344618","https://openalex.org/I98677209"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Abram Giller","raw_affiliation_strings":["Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040957546","display_name":"Kevin Myant","orcid":"https://orcid.org/0000-0001-8017-1093","observed_orcids":["https://orcid.org/0000-0001-8017-1093"]},"institutions":[{"id":"https://openalex.org/I4210158898","display_name":"Institute of Genetics and Cancer","ror":"https://ror.org/05hygey35","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210087105","https://openalex.org/I4210158898","https://openalex.org/I90344618","https://openalex.org/I98677209"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Kevin B Myant","raw_affiliation_strings":["Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086520308","display_name":"Sara Mac\u00edas","orcid":"https://orcid.org/0000-0002-0643-3494","observed_orcids":["https://orcid.org/0000-0002-0643-3494","https://orcid.org/0000-0003-1677-7685"]},"institutions":[{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sara Macias","raw_affiliation_strings":["Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, UK"],"raw_orcid":"https://orcid.org/0000-0002-0643-3494","affiliations":[{"raw_affiliation_string":"Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I98677209"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037023019","display_name":"Andrew J. Wood","orcid":"https://orcid.org/0000-0002-0653-2070","observed_orcids":["https://orcid.org/0000-0002-0653-2070","https://orcid.org/0000-0003-4850-6345"]},"institutions":[{"id":"https://openalex.org/I4210158898","display_name":"Institute of Genetics and Cancer","ror":"https://ror.org/05hygey35","country_code":"GB","type":"facility","lineage":["https://openalex.org/I4210087105","https://openalex.org/I4210158898","https://openalex.org/I90344618","https://openalex.org/I98677209"]},{"id":"https://openalex.org/I98677209","display_name":"University of Edinburgh","ror":"https://ror.org/01nrxwf90","country_code":"GB","type":"education","lineage":["https://openalex.org/I98677209"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Andrew J Wood","raw_affiliation_strings":["Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK"],"raw_orcid":"https://orcid.org/0000-0002-0653-2070","affiliations":[{"raw_affiliation_string":"Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK","institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5037023019"],"corresponding_institution_ids":["https://openalex.org/I4210158898","https://openalex.org/I98677209"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12534","display_name":"Protein Degradation and Inhibitors","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.7580000162124634},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.3799999952316284},{"id":"https://openalex.org/keywords/aid","display_name":"AID","score":0.4050000011920929},{"id":"https://openalex.org/keywords/dtag","display_name":"DTAG","score":0.9520000219345093},{"id":"https://openalex.org/keywords/smash","display_name":"smash","score":0.984000027179718},{"id":"https://openalex.org/keywords/organoids","display_name":"organoids","score":0.609000027179718},{"id":"https://openalex.org/keywords/drug-selection","display_name":"drug selection","score":0.5830000042915344},{"id":"https://openalex.org/keywords/target-validation","display_name":"target validation","score":0.5740000009536743}],"concepts":[{"id":"https://openalex.org/C2775895488","wikidata":"https://www.wikidata.org/wiki/Q5251856","display_name":"Degron","level":5,"score":0.7304999828338623},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.636900007724762},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5957000255584717},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.5795999765396118},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.546500027179718},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.5313000082969666},{"id":"https://openalex.org/C31695470","wikidata":"https://www.wikidata.org/wiki/Q11293125","display_name":"Organoid","level":2,"score":0.48429998755455017},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.46869999170303345},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4453999996185303},{"id":"https://openalex.org/C7386963","wikidata":"https://www.wikidata.org/wiki/Q3954859","display_name":"Negative selection","level":4,"score":0.43369999527931213},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.40860000252723694},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.3887999951839447},{"id":"https://openalex.org/C2909909245","wikidata":"https://www.wikidata.org/wiki/Q412550","display_name":"Integrases","level":4,"score":0.3804999887943268},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3734999895095825},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.3481999933719635},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.3456999957561493},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.32850000262260437},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.3098999857902527},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.2994000017642975},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.2924000024795532},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.2915000021457672},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2851000130176544},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2815000116825104},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.27480000257492065},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.26579999923706055},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.25429999828338623},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.2506999969482422}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.14.751494","doi":"https://doi.org/10.64898/2026.09.14.751494","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.14.751494","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.14.751494.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.14.751494","doi":"https://doi.org/10.64898/2026.09.14.751494","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.14.751494","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/17/2026.09.14.751494.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.504800021648407,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1085281164","display_name":"Contribution of the miRNA-interferon interaction to human disease","funder_award_id":"221737","funder_id":"https://openalex.org/F4320311904","funder_display_name":"Wellcome Trust"},{"id":"https://openalex.org/G6996719166","display_name":"Contribution of the miRNA-interferon interaction to human disease","funder_award_id":"221737/Z/20/Z","funder_id":"https://openalex.org/F4320311904","funder_display_name":"Wellcome Trust"},{"id":"https://openalex.org/G7721925949","display_name":"Degron - Precision control of protein dosage in vivo","funder_award_id":"MC_PC_21040","funder_id":"https://openalex.org/F4320334626","funder_display_name":"Medical Research Council"}],"funders":[{"id":"https://openalex.org/F4320311904","display_name":"Wellcome Trust","ror":"https://ror.org/029chgv08"},{"id":"https://openalex.org/F4320334626","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213459208.pdf","grobid_xml":"https://content.openalex.org/works/W7213459208.grobid-xml"},"referenced_works_count":2,"referenced_works":["https://openalex.org/W4405323257","https://openalex.org/W4415486672"],"related_works":["https://openalex.org/W2419278777","https://openalex.org/W3166430562","https://openalex.org/W7161941490","https://openalex.org/W2395322534","https://openalex.org/W4378700637","https://openalex.org/W4312733294","https://openalex.org/W3217041439","https://openalex.org/W7138882499","https://openalex.org/W4295115622","https://openalex.org/W3112199093"],"abstract_inverted_index":{"Abstract":[0],"Degron":[1],"tags":[2,68,83,123],"enable":[3,62],"rapid,":[4],"tuneable":[5],"and":[6,12,21,40,111,124],"reversible":[7],"control":[8],"of":[9,57,65,121],"protein":[10,90],"expression,":[11],"have":[13,53],"become":[14],"a":[15,55],"critical":[16],"tool":[17],"for":[18,48],"target":[19],"validation":[20],"elucidating":[22],"biological":[23],"mechanisms.":[24],"Tag":[25],"engineering":[26,120],"uses":[27],"genome":[28],"editing":[29],"to":[30,45,61,88],"generate":[31],"biallelic":[32,63],"gene":[33],"fusions,":[34],"though":[35],"this":[36,81],"process":[37],"remains":[38],"laborious":[39],"requires":[41],"new":[42],"donor":[43,59],"vectors":[44,60],"be":[46,85],"generated":[47,54],"each":[49],"locus.":[50],"Here,":[51],"we":[52],"series":[56],"universal":[58],"integration":[64],"different":[66,74],"degron":[67,122],"(AID,":[69],"dTAG,":[70],"BromoTag,":[71],"SMASh)":[72],"using":[73,103],"selection":[75],"strategies":[76],"(FACS,":[77],"drug":[78],"selection).":[79],"Using":[80],"system,":[82],"can":[84],"fused":[86],"in-frame":[87],"endogenous":[89],"C-termini":[91],"via":[92],"homology-independent":[93],"repair":[94,131],"following":[95],"Cas9-induced":[96],"double":[97],"strand":[98],"breaks,":[99],"as":[100],"shown":[101],"here":[102],"human":[104],"lymphoma":[105],"cells,":[106],"mouse":[107],"embryonic":[108],"stem":[109],"cells":[110],"colonic":[112],"tumour":[113],"organoids.":[114],"This":[115],"workflow":[116],"simplifies":[117],"the":[118],"genetic":[119],"is":[125,132],"applicable":[126],"in":[127],"contexts":[128],"where":[129],"homology-dependent":[130],"challenging.":[133]},"counts_by_year":[],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2026-09-17T00:00:00"}
