{"id":"https://openalex.org/W7213445619","doi":"https://doi.org/10.64898/2026.09.15.751771","title":"Multi-agentic system for primer design in qPCR and LAMP diagnostics tests","display_name":"Multi-agentic system for primer design in qPCR and LAMP diagnostics tests","publication_year":2026,"publication_date":"2026-09-16","ids":{"openalex":"https://openalex.org/W7213445619","doi":"https://doi.org/10.64898/2026.09.15.751771"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.15.751771","doi":"https://doi.org/10.64898/2026.09.15.751771","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.15.751771","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/16/2026.09.15.751771.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/16/2026.09.15.751771.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112707772","display_name":"Kenny Jx Lau","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162266279","display_name":"Temasek Life Sciences Laboratory","ror":"https://ror.org/0574eex11","country_code":"SG","type":"facility","lineage":["https://openalex.org/I162266279"]},{"id":"https://openalex.org/I165932596","display_name":"National University of Singapore","ror":"https://ror.org/01tgyzw49","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596"]}],"countries":["SG"],"is_corresponding":true,"raw_author_name":"Kenny J.X. Lau","raw_affiliation_strings":["Institute of Systems Science, National University of Singapore, Singapore 119615","Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Systems Science, National University of Singapore, Singapore 119615","institution_ids":["https://openalex.org/I165932596"]},{"raw_affiliation_string":"Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604","institution_ids":["https://openalex.org/I162266279","https://openalex.org/I165932596"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5112707772"],"corresponding_institution_ids":["https://openalex.org/I162266279","https://openalex.org/I165932596"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.5791000127792358,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.5791000127792358,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.2215999960899353,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.05849999934434891,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/primer-design","display_name":"primer design","score":0.9670000076293945},{"id":"https://openalex.org/keywords/loop-mediated-isothermal-amplification","display_name":"loop-mediated isothermal amplification","score":0.9509999752044678},{"id":"https://openalex.org/keywords/multi-agent-systems","display_name":"multi-agent systems","score":0.8640000224113464},{"id":"https://openalex.org/keywords/ai-orchestration","display_name":"AI orchestration","score":0.4620000123977661},{"id":"https://openalex.org/keywords/langgraph","display_name":"LangGraph","score":0.7210000157356262},{"id":"https://openalex.org/keywords/langchain","display_name":"LangChain","score":0.7229999899864197},{"id":"https://openalex.org/keywords/automated-bioinformatics-workflow","display_name":"automated bioinformatics workflow","score":0.6919999718666077},{"id":"https://openalex.org/keywords/multiple-sequence-alignment","display_name":"multiple sequence alignment","score":0.8709999918937683},{"id":"https://openalex.org/keywords/conserved-genomic-regions","display_name":"conserved genomic regions","score":0.9649999737739563},{"id":"https://openalex.org/keywords/molecular-diagnosis","display_name":"molecular diagnosis","score":0.6790000200271606},{"id":"https://openalex.org/keywords/infectious-disease-diagnostics","display_name":"infectious disease diagnostics","score":0.7940000295639038},{"id":"https://openalex.org/keywords/outbreak-surveillance","display_name":"outbreak surveillance","score":0.7749999761581421},{"id":"https://openalex.org/keywords/environmental-monitoring","display_name":"environmental monitoring","score":0.9729999899864197}],"concepts":[{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7110000252723694},{"id":"https://openalex.org/C60635243","wikidata":"https://www.wikidata.org/wiki/Q6675780","display_name":"Loop-mediated isothermal amplification","level":3,"score":0.6998000144958496},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.6890000104904175},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5972999930381775},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.48240000009536743},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.47540000081062317},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.3962000012397766},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36970001459121704},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.36719998717308044},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.3483999967575073},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.3327000141143799},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.329800009727478},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.31290000677108765},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.30660000443458557},{"id":"https://openalex.org/C200658500","wikidata":"https://www.wikidata.org/wiki/Q4112082","display_name":"In silico PCR","level":5,"score":0.2987000048160553},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.2944999933242798},{"id":"https://openalex.org/C88031987","wikidata":"https://www.wikidata.org/wiki/Q1377767","display_name":"Multiple sequence alignment","level":5,"score":0.28600001335144043},{"id":"https://openalex.org/C45484198","wikidata":"https://www.wikidata.org/wiki/Q827246","display_name":"Sequence alignment","level":4,"score":0.28049999475479126},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.27869999408721924},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.26840001344680786},{"id":"https://openalex.org/C2779315201","wikidata":"https://www.wikidata.org/wiki/Q414601","display_name":"Sequence homology","level":4,"score":0.25429999828338623},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.2533999979496002},{"id":"https://openalex.org/C41584329","wikidata":"https://www.wikidata.org/wiki/Q175902","display_name":"Sequence database","level":3,"score":0.25049999356269836}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.15.751771","doi":"https://doi.org/10.64898/2026.09.15.751771","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.15.751771","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/16/2026.09.15.751771.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.15.751771","doi":"https://doi.org/10.64898/2026.09.15.751771","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.15.751771","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/16/2026.09.15.751771.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213445619.pdf","grobid_xml":"https://content.openalex.org/works/W7213445619.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4388209783","https://openalex.org/W4393317387","https://openalex.org/W2346340297","https://openalex.org/W7213306121","https://openalex.org/W4416856554","https://openalex.org/W4403873631","https://openalex.org/W4411772098","https://openalex.org/W7170801202","https://openalex.org/W7160684464","https://openalex.org/W3000699638"],"abstract_inverted_index":{"ABSTRACT":[0],"Primer":[1],"design":[2,25,132],"is":[3,26],"a":[4,75],"fundamental":[5],"component":[6],"of":[7,77,125],"molecular":[8],"diagnostics":[9],"in":[10,142],"both":[11],"quantitative":[12],"polymerase":[13],"chain":[14],"reaction":[15],"(qPCR)":[16],"and":[17,36,60,63,72,110,148,168],"loop-mediated":[18],"isothermal":[19],"amplification":[20],"(LAMP)":[21],"assays.":[22],"However,":[23],"assay":[24,138,170],"often":[27],"performed":[28],"manually":[29],"as":[30],"nucleotide":[31,108],"databases,":[32],"sequence":[33,113,120],"alignment":[34,121],"tools":[35],"resources":[37],"are":[38],"found":[39],"at":[40],"different":[41],"places":[42],"on":[43,97],"the":[44,57,98,102,111,123],"Internet.":[45],"In":[46],"this":[47],"study,":[48],"an":[49],"AI-orchestrated":[50],"bioinformatics":[51,85,163],"workflow":[52,66],"was":[53,67],"developed":[54],"to":[55,82,118,165],"automate":[56,166],"end-to-end":[58],"qPCR":[59,167],"LAMP":[61,169],"primers":[62],"probes.":[64],"The":[65,129],"implemented":[68],"using":[69],"LangGraph,":[70],"LangChain":[71],"Biopython,":[73],"where":[74],"series":[76],"specialized":[78],"agents":[79],"were":[80,93,115],"coordinated":[81],"execute":[83],"sequential":[84],"tasks":[86],"with":[87,161],"minimal":[88],"human":[89],"intervention.":[90],"Target":[91],"sequences":[92],"then":[94,116],"retrieved":[95],"based":[96],"user\u2019s":[99],"request":[100],"from":[101],"National":[103],"Center":[104],"for":[105,122,137,140],"Biotechnology":[106],"Information":[107],"database":[109],"requested":[112],"records":[114],"subjected":[117],"multiple":[119],"identification":[124],"conserved":[126],"genomic":[127],"regions.":[128],"multi-agentic":[130,156],"primer":[131],"system":[133],"can":[134,158],"be":[135,159],"used":[136],"development":[139],"applications":[141],"infectious":[143],"disease":[144],"diagnostics,":[145],"outbreak":[146],"surveillance":[147],"environmental":[149],"monitoring.":[150],"This":[151],"study":[152],"also":[153],"demonstrates":[154],"how":[155],"systems":[157],"combined":[160],"established":[162],"methods":[164],"design.":[171]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-09-17T00:00:00"}