{"id":"https://openalex.org/W7213354827","doi":"https://doi.org/10.21769/bioprotoc.5839","title":"Optimized Phenol\u2013Chloroform\u2013Isoamyl DNA Extraction Protocol for Single Fish Eggs","display_name":"Optimized Phenol\u2013Chloroform\u2013Isoamyl DNA Extraction Protocol for Single Fish Eggs","publication_year":2026,"publication_date":"2026-01-01","ids":{"openalex":"https://openalex.org/W7213354827","doi":"https://doi.org/10.21769/bioprotoc.5839","pmid":"https://pubmed.ncbi.nlm.nih.gov/42947749","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13710124"},"language":"en","primary_location":{"id":"doi:10.21769/bioprotoc.5839","doi":"https://doi.org/10.21769/bioprotoc.5839","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.5839","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.21769/bioprotoc.5839","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5151773160","display_name":"Iva Vrgo&ccaron;","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156528","display_name":"Institute of Oceanography and Fisheries","ror":"https://ror.org/04ma0p518","country_code":"HR","type":"facility","lineage":["https://openalex.org/I4210156528"]}],"countries":["HR"],"is_corresponding":true,"raw_author_name":"Iva Vrgo&ccaron;","raw_affiliation_strings":["Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia","institution_ids":["https://openalex.org/I4210156528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151737963","display_name":"Ivana Plei&cacute;","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156528","display_name":"Institute of Oceanography and Fisheries","ror":"https://ror.org/04ma0p518","country_code":"HR","type":"facility","lineage":["https://openalex.org/I4210156528"]}],"countries":["HR"],"is_corresponding":false,"raw_author_name":"Ivana Plei&cacute;","raw_affiliation_strings":["Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia","institution_ids":["https://openalex.org/I4210156528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151729245","display_name":"Klara Ivani\u0161evi&cacute;","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156528","display_name":"Institute of Oceanography and Fisheries","ror":"https://ror.org/04ma0p518","country_code":"HR","type":"facility","lineage":["https://openalex.org/I4210156528"]}],"countries":["HR"],"is_corresponding":false,"raw_author_name":"Klara Ivani\u0161evi&cacute;","raw_affiliation_strings":["Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia","institution_ids":["https://openalex.org/I4210156528"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5151825361","display_name":"Tanja Bubi&cacute;","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156528","display_name":"Institute of Oceanography and Fisheries","ror":"https://ror.org/04ma0p518","country_code":"HR","type":"facility","lineage":["https://openalex.org/I4210156528"]}],"countries":["HR"],"is_corresponding":false,"raw_author_name":"Tanja Bubi&cacute;","raw_affiliation_strings":["Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Oceanography and Fisheries, \u0160etali\u0161te Ivana Me\u0161trovi\u0107a 63, Split, Croatia","institution_ids":["https://openalex.org/I4210156528"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5151773160"],"corresponding_institution_ids":["https://openalex.org/I4210156528"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.62011556,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"16","issue":"1407","first_page":"e5839","last_page":"e5839"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.42089998722076416,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.42089998722076416,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12388","display_name":"Identification and Quantification in Food","score":0.4133000075817108,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0478999987244606,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-extraction","display_name":"DNA extraction","score":0.7609999775886536},{"id":"https://openalex.org/keywords/fish-eggs","display_name":"fish eggs","score":0.9829999804496765},{"id":"https://openalex.org/keywords/mitochondrial-coi-gene","display_name":"mitochondrial COI gene","score":0.8650000095367432},{"id":"https://openalex.org/keywords/species-identification","display_name":"species identification","score":0.9160000085830688},{"id":"https://openalex.org/keywords/low-cost-protocol","display_name":"low-cost protocol","score":0.5910000205039978}],"concepts":[{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.46480000019073486},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.4307999908924103},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41670000553131104},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.3785000145435333},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3488999903202057},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.32260000705718994},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.3052999973297119},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.2802000045776367},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.2766000032424927},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2578999996185303},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.2574999928474426},{"id":"https://openalex.org/C39432304","wikidata":"https://www.wikidata.org/wiki/Q188847","display_name":"Environmental science","level":0,"score":0.2531000077724457}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.21769/bioprotoc.5839","doi":"https://doi.org/10.21769/bioprotoc.5839","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.5839","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13710124","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13710124/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13710124/pdf/BioProtoc-16-20-5839.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio Protoc","raw_type":"Text"},{"id":"pmid:42947749","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42947749","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio-protocol","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:9c9a2fa896de4a34b1af8b76d5d2b6c2","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/9c9a2fa896de4a34b1af8b76d5d2b6c2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bio-Protocol, Vol 16, Iss 20 (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.21769/bioprotoc.5839","doi":"https://doi.org/10.21769/bioprotoc.5839","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.5839","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","oarl","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3408410167","display_name":null,"funder_award_id":"IP-2022-10","funder_id":"https://openalex.org/F4320322674","funder_display_name":"Hrvatska Zaklada za Znanost"},{"id":"https://openalex.org/G4268986784","display_name":null,"funder_award_id":"HRZZ-IP-2022-10-7232","funder_id":"https://openalex.org/F4320322674","funder_display_name":"Hrvatska Zaklada za Znanost"},{"id":"https://openalex.org/G623446314","display_name":"Enhancing Environmental Performance of Net-Pen Marine Aquaculture","funder_award_id":"IP-2022-10-7232","funder_id":"https://openalex.org/F4320322674","funder_display_name":"Hrvatska Zaklada za Znanost"}],"funders":[{"id":"https://openalex.org/F4320322674","display_name":"Hrvatska Zaklada za Znanost","ror":"https://ror.org/03n51vw80"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213354827.pdf","grobid_xml":"https://content.openalex.org/works/W7213354827.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2810640349","https://openalex.org/W2045143604","https://openalex.org/W6922801543","https://openalex.org/W1578426293","https://openalex.org/W2329635043","https://openalex.org/W4388071188","https://openalex.org/W4243059764","https://openalex.org/W2736903527","https://openalex.org/W2270864779","https://openalex.org/W6941426068"],"abstract_inverted_index":{")":[0],"by":[1,23],"modifying":[2],"buffer":[3],"volumes,":[4],"incubation":[5],"times,":[6],"and":[7,33,41,55,68],"washing":[8],"steps,":[9],"following":[10],"prior":[11],"micropuncturing":[12],"of":[13,27],"eggs":[14],"on":[15],"a":[16,39],"glass":[17],"slide.":[18],"DNA":[19,57],"quality":[20],"is":[21],"confirmed":[22],"spectrophotometry,":[24],"PCR":[25,78],"amplification":[26],"the":[28],"mitochondrial":[29],"COI":[30],"gene,":[31],"electrophoresis,":[32],"Sanger":[34],"sequencing.":[35],"This":[36],"method":[37,59],"provides":[38],"low-cost":[40,56],"effective":[42],"approach":[43],"for":[44],"species":[45],"identification":[46],"from":[47,60],"individual":[48],"fish":[49,61],"eggs.":[50,62],"Key":[51],"features":[52],"\u2022":[53,63,76],"Effective":[54],"extraction":[58],"Uses":[64],"only":[65],"standard":[66],"reagents":[67],"equipment,":[69],"allowing":[70],"wide":[71],"application":[72],"in":[73],"all":[74],"laboratories.":[75],"High":[77],"efficiency.":[79]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-09-16T00:00:00"}