{"id":"https://openalex.org/W7213118121","doi":"https://doi.org/10.21203/rs.3.rs-11006577/v1","title":"Bioproduction of Neospora caninum: a proof-of- concept towards a scalable serum-free process","display_name":"Bioproduction of Neospora caninum: a proof-of- concept towards a scalable serum-free process","publication_year":2026,"publication_date":"2026-09-14","ids":{"openalex":"https://openalex.org/W7213118121","doi":"https://doi.org/10.21203/rs.3.rs-11006577/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11006577/v1","doi":"https://doi.org/10.21203/rs.3.rs-11006577/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11006577/v1","pdf_url":"https://www.researchsquare.com/article/rs-11006577/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-11006577/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025691540","display_name":"Zineb Lakhrif","orcid":"https://orcid.org/0000-0001-9010-8493","observed_orcids":["https://orcid.org/0000-0001-9010-8493"]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Zineb Lakhrif","raw_affiliation_strings":["Universit\u00e9 de Tours, INRAE, ISP"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Tours, INRAE, ISP","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I4210088668"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005543801","display_name":"Nathalie Moir\u00e9","orcid":"https://orcid.org/0000-0001-9171-5711","observed_orcids":["https://orcid.org/0000-0001-9171-5711"]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Nathalie Moir\u00e9","raw_affiliation_strings":["INRAE, Universit\u00e9 de Tours, ISP"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"INRAE, Universit\u00e9 de Tours, ISP","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I4210088668"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151681578","display_name":"Audrey Munos-Guillon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Audrey Munos-Guillon","raw_affiliation_strings":["BioCube Institute, Groupe IMT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BioCube Institute, Groupe IMT","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Anne di Tommaso","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Anne di Tommaso","raw_affiliation_strings":["Universit\u00e9 de Tours, INRAE, ISP"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Tours, INRAE, ISP","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I4210088668"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058764161","display_name":"C\u00e9line Ducournau","orcid":"https://orcid.org/0000-0002-0920-2392","observed_orcids":["https://orcid.org/0000-0002-0920-2392"]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"C\u00e9line Ducournau","raw_affiliation_strings":["Universit\u00e9 de Tours, INRAE, ISP"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Tours, INRAE, ISP","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I4210088668"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059513386","display_name":"Isabelle Dimier\u2010Poisson","orcid":"https://orcid.org/0000-0001-8917-4493","observed_orcids":["https://orcid.org/0000-0001-8917-4493"]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Isabelle Dimier-Poisson","raw_affiliation_strings":["Universit\u00e9 de Tours, INRAE, ISP"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Tours, INRAE, ISP","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I4210088668"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073243780","display_name":"Cyril Poupet","orcid":"https://orcid.org/0000-0001-9343-4390","observed_orcids":["https://orcid.org/0000-0001-9343-4390"]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Cyril Poupet","raw_affiliation_strings":["Universit\u00e9 de Tours, INRAE, ISP"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e9 de Tours, INRAE, ISP","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I4210088668"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.7343999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.7343999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11155","display_name":"Toxoplasma gondii Research Studies","score":0.2515000104904175,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neospora-caninum","display_name":"Neospora caninum","score":1.0},{"id":"https://openalex.org/keywords/serum-free-culture","display_name":"serum-free culture","score":0.859000027179718},{"id":"https://openalex.org/keywords/cho-k1-cells","display_name":"CHO-K1 cells","score":0.9160000085830688},{"id":"https://openalex.org/keywords/bhk-21-cells","display_name":"BHK-21 cells","score":0.9980000257492065},{"id":"https://openalex.org/keywords/defined-culture-medium","display_name":"defined culture medium","score":0.5849999785423279},{"id":"https://openalex.org/keywords/host-parasite-metabolic-interactions","display_name":"host-parasite metabolic interactions","score":0.7670000195503235},{"id":"https://openalex.org/keywords/multiplicity-of-infection","display_name":"multiplicity of infection","score":0.7749999761581421},{"id":"https://openalex.org/keywords/gmp-manufacturing","display_name":"GMP manufacturing","score":0.5979999899864197},{"id":"https://openalex.org/keywords/cancer-immunotherapy","display_name":"cancer immunotherapy","score":0.7039999961853027}],"concepts":[{"id":"https://openalex.org/C2779227428","wikidata":"https://www.wikidata.org/wiki/Q4915219","display_name":"Bioproduction","level":2,"score":0.7978000044822693},{"id":"https://openalex.org/C2778566189","wikidata":"https://www.wikidata.org/wiki/Q101274","display_name":"Protozoa","level":2,"score":0.7135999798774719},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.692300021648407},{"id":"https://openalex.org/C2781154746","wikidata":"https://www.wikidata.org/wiki/Q1431408","display_name":"Neospora caninum","level":4,"score":0.6662999987602234},{"id":"https://openalex.org/C24990904","wikidata":"https://www.wikidata.org/wiki/Q7075144","display_name":"Obligate","level":2,"score":0.48730000853538513},{"id":"https://openalex.org/C2776576699","wikidata":"https://www.wikidata.org/wiki/Q487837","display_name":"Coccidiosis","level":2,"score":0.4334000051021576},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4011000096797943},{"id":"https://openalex.org/C2780513914","wikidata":"https://www.wikidata.org/wiki/Q18210350","display_name":"Bottleneck","level":2,"score":0.3871000111103058},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3425999879837036},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.3361999988555908},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.33250001072883606},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3249000012874603},{"id":"https://openalex.org/C4916135","wikidata":"https://www.wikidata.org/wiki/Q4119055","display_name":"Bioprocess","level":2,"score":0.32109999656677246},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.31850001215934753},{"id":"https://openalex.org/C2775893736","wikidata":"https://www.wikidata.org/wiki/Q25324110","display_name":"Biomanufacturing","level":2,"score":0.299699991941452},{"id":"https://openalex.org/C99389464","wikidata":"https://www.wikidata.org/wiki/Q3319949","display_name":"Gametocyte","level":4,"score":0.2980000078678131},{"id":"https://openalex.org/C2778675580","wikidata":"https://www.wikidata.org/wiki/Q614956","display_name":"Plasmodium (life cycle)","level":3,"score":0.29760000109672546},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.2937999963760376},{"id":"https://openalex.org/C82210918","wikidata":"https://www.wikidata.org/wiki/Q1560099","display_name":"Oncolytic virus","level":3,"score":0.288100004196167},{"id":"https://openalex.org/C153726182","wikidata":"https://www.wikidata.org/wiki/Q3720909","display_name":"Intracellular parasite","level":3,"score":0.2802000045776367},{"id":"https://openalex.org/C2776455988","wikidata":"https://www.wikidata.org/wiki/Q353172","display_name":"Protozoan infection","level":2,"score":0.27079999446868896},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.2700999975204468},{"id":"https://openalex.org/C3019792204","wikidata":"https://www.wikidata.org/wiki/Q353172","display_name":"Protozoan parasite","level":3,"score":0.2669000029563904},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.2635999917984009}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.21203/rs.3.rs-11006577/v1","doi":"https://doi.org/10.21203/rs.3.rs-11006577/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11006577/v1","pdf_url":"https://www.researchsquare.com/article/rs-11006577/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},{"id":"pmh:oai:HAL:hal-05805966v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.science/hal-05805966","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"2026","raw_type":"info:eu-repo/semantics/preprint"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11006577/v1","doi":"https://doi.org/10.21203/rs.3.rs-11006577/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11006577/v1","pdf_url":"https://www.researchsquare.com/article/rs-11006577/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7213118121.pdf","grobid_xml":"https://content.openalex.org/works/W7213118121.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W1828106707","https://openalex.org/W1841145069","https://openalex.org/W1889799197","https://openalex.org/W1976208842","https://openalex.org/W1999195296","https://openalex.org/W2005835980","https://openalex.org/W2031556861","https://openalex.org/W2036850278","https://openalex.org/W2052244601","https://openalex.org/W2067478890","https://openalex.org/W2069703326","https://openalex.org/W2088023218","https://openalex.org/W2100470475","https://openalex.org/W2100577789","https://openalex.org/W2114671362","https://openalex.org/W2115145177","https://openalex.org/W2131314467","https://openalex.org/W2193922054","https://openalex.org/W2409538367","https://openalex.org/W2563175358","https://openalex.org/W2597879482","https://openalex.org/W2603639392","https://openalex.org/W2742871720","https://openalex.org/W2887436702","https://openalex.org/W2906876127","https://openalex.org/W2990128831","https://openalex.org/W3002607242","https://openalex.org/W3082826811","https://openalex.org/W3107443730","https://openalex.org/W3107748207","https://openalex.org/W3162456054","https://openalex.org/W3165099066","https://openalex.org/W3197932110","https://openalex.org/W4282967229","https://openalex.org/W4296793670","https://openalex.org/W4320490407","https://openalex.org/W4376871919","https://openalex.org/W4392353806","https://openalex.org/W4392711440","https://openalex.org/W4400418577","https://openalex.org/W4409974541","https://openalex.org/W4411227706"],"related_works":["https://openalex.org/W7214692426","https://openalex.org/W7206610255","https://openalex.org/W79033756","https://openalex.org/W2131314467","https://openalex.org/W7037785104","https://openalex.org/W4388789077","https://openalex.org/W7097796120","https://openalex.org/W4399250022","https://openalex.org/W2091365100","https://openalex.org/W7047035926"],"abstract_inverted_index":{"Abstract":[0],"Neospora":[1],"caninum":[2,61,124,209],"is":[3,62],"emerging":[4],"as":[5,220],"a":[6,21,64,72,159,221],"promising":[7,222],"oncolytic":[8],"protozoan":[9],"for":[10,41,59,67,98,224],"cancer":[11],"immunotherapy.":[12],"However,":[13],"large-scale":[14],"production":[15,143,172,210],"of":[16,80,128,206],"tachyzoites":[17],"remains":[18],"constrained":[19],"by":[20,176],"culture":[22],"system":[23],"dependent":[24],"on":[25,71],"animal-derived":[26],"serum":[27],"and":[28,36,47,52,83,93,102,111,120,136,145,179,189,216,227],"primary-like":[29],"host":[30,133],"cells,":[31],"limiting":[32],"standardisation,":[33],"regulatory":[34,74],"compliance,":[35],"scalability.":[37,113],"Moreover,":[38],"bioproduction":[39],"processes":[40],"obligate":[42],"intracellular":[43],"protozoa":[44,99,153,187],"are":[45],"scarce":[46],"largely":[48],"restricted":[49],"to":[50,107,141],"Plasmodium":[51],"Leishmania":[53],".":[54],"Developing":[55],"scalable":[56],"manufacturing":[57,84],"strategies":[58],"N.":[60,123,208],"therefore":[63],"critical":[65],"bottleneck":[66],"clinical":[68],"translation.":[69],"Building":[70],"prior":[73],"analysis,":[75],"which":[76],"emphasised":[77],"early":[78],"integration":[79],"classification,":[81],"quality,":[82],"constraints,":[85],"we":[86],"evaluated":[87],"industrially":[88],"relevant":[89],"mammalian":[90],"cell":[91,134,150,213],"lines":[92,151,214],"serum-reduced":[94],"or":[95],"serum-free":[96,156,207],"conditions":[97],"production.":[100],"BHK-21":[101,165],"CHO-K1":[103,163],"cells":[104],"were":[105,115,139],"selected":[106],"balance":[108],"physiological":[109],"relevance":[110],"industrial":[112,212],"Cells":[114],"cultured":[116],"in":[117,166],"defined":[118],"media":[119],"infected":[121],"with":[122,158,162,181,194],"at":[125],"varying":[126],"multiplicities":[127],"infection":[129,197],"(MOI).":[130],"Protozoa":[131],"yield,":[132],"growth,":[135],"glucose/lactate":[137],"dynamics":[138],"monitored":[140],"assess":[142],"performance":[144],"host\u2013protozoa":[146],"metabolic":[147,191],"interactions.":[148],"Both":[149],"supported":[152],"multiplication":[154],"under":[155],"conditions,":[157],"better":[160],"yield":[161],"than":[164],"our":[167],"experimental":[168],"conditions.":[169],"In":[170],"CHO-K1,":[171],"was":[173],"strongly":[174],"influenced":[175],"medium":[177,223],"composition":[178],"MOI,":[180],"Advanced":[182,218],"DMEM/F-12":[183,219],"yielding":[184],"the":[185,204],"highest":[186],"output":[188],"stable":[190],"profiles,":[192],"while":[193],"other":[195],"media,":[196],"increased":[198],"glucose":[199],"consumption.":[200],"These":[201],"findings":[202],"demonstrate":[203],"feasibility":[205],"using":[211],"(CHO-K1)":[215],"identify":[217],"scalable,":[225],"standardised,":[226],"GMP-compatible":[228],"manufacturing.":[229]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-09-15T00:00:00"}