{"id":"https://openalex.org/W7212974107","doi":"https://doi.org/10.64898/2026.09.07.749981","title":"Integrated Spatial Metabolomics and Proteomics from the Same Tissue Section Using a Conductive ITO-PET Slide","display_name":"Integrated Spatial Metabolomics and Proteomics from the Same Tissue Section Using a Conductive ITO-PET Slide","publication_year":2026,"publication_date":"2026-09-14","ids":{"openalex":"https://openalex.org/W7212974107","doi":"https://doi.org/10.64898/2026.09.07.749981"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.07.749981","doi":"https://doi.org/10.64898/2026.09.07.749981","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.07.749981","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.07.749981.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.07.749981.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5151615677","display_name":"haizhu wang","orcid":"https://orcid.org/0009-0000-0238-1431","observed_orcids":["https://orcid.org/0009-0000-0238-1431"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210112540","display_name":"Institute of Biophysics","ror":"https://ror.org/01tyv8576","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210112540"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Haizhu Wang","raw_affiliation_strings":["Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210112540"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151696721","display_name":"Yan Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405252760","display_name":"Guangzhou Laboratory","ror":"https://ror.org/03ybmxt82","country_code":"CN","type":"government","lineage":["https://openalex.org/I4405252760"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shengmei Liu","raw_affiliation_strings":["Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China","institution_ids":["https://openalex.org/I4405252760"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5134597058","display_name":"Peng Xue","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405252760","display_name":"Guangzhou Laboratory","ror":"https://ror.org/03ybmxt82","country_code":"CN","type":"government","lineage":["https://openalex.org/I4405252760"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lixian Lin","raw_affiliation_strings":["Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China","institution_ids":["https://openalex.org/I4405252760"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5152788049","display_name":"Yongsheng Cheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210160531","display_name":"Chinese People's Liberation Army","ror":"https://ror.org/05tf9r976","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210160531"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yongsheng Cheng","raw_affiliation_strings":["The Air Force Hospital of the Southern Theater Command of the Chinese People\u2019s Liberation Army, 801 Dongfeng East Road, Yuexiu District, Guangzhou 510062, Guangdong, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Air Force Hospital of the Southern Theater Command of the Chinese People\u2019s Liberation Army, 801 Dongfeng East Road, Yuexiu District, Guangzhou 510062, Guangdong, China","institution_ids":["https://openalex.org/I4210160531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5152943257","display_name":"Yuchuang Zheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405252760","display_name":"Guangzhou Laboratory","ror":"https://ror.org/03ybmxt82","country_code":"CN","type":"government","lineage":["https://openalex.org/I4405252760"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuchuang Zheng","raw_affiliation_strings":["Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China","institution_ids":["https://openalex.org/I4405252760"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5152864066","display_name":"Yan Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210112540","display_name":"Institute of Biophysics","ror":"https://ror.org/01tyv8576","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210112540"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yan Li","raw_affiliation_strings":["Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210112540"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100784171","display_name":"Peng Xue","orcid":"https://orcid.org/0000-0001-6844-6066","observed_orcids":["https://orcid.org/0000-0001-6844-6066"]},"institutions":[{"id":"https://openalex.org/I4405252760","display_name":"Guangzhou Laboratory","ror":"https://ror.org/03ybmxt82","country_code":"CN","type":"government","lineage":["https://openalex.org/I4405252760"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Peng Xue","raw_affiliation_strings":["Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical-Engineering Integration and Technology, Guangzhou Laboratory, 9 Xingdao Ring North Road, Guangzhou International Bio Island, Haizhu District, Guangzhou 510005, China","institution_ids":["https://openalex.org/I4405252760"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5152864066"],"corresponding_institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210112540"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.019200000911951065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.01510000042617321,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/indium-tin-oxide","display_name":"indium tin oxide","score":0.8190000057220459},{"id":"https://openalex.org/keywords/maldi-mass-spectrometry-imaging","display_name":"MALDI mass spectrometry imaging","score":0.8169999718666077},{"id":"https://openalex.org/keywords/laser-capture-microdissection","display_name":"laser capture microdissection","score":0.9919999837875366},{"id":"https://openalex.org/keywords/spatial-metabolomics","display_name":"spatial metabolomics","score":0.7400000095367432},{"id":"https://openalex.org/keywords/spatial-proteomics","display_name":"spatial proteomics","score":0.996999979019165},{"id":"https://openalex.org/keywords/mouse-brain","display_name":"mouse brain","score":0.5740000009536743},{"id":"https://openalex.org/keywords/tissue-preservation","display_name":"tissue preservation","score":0.32600000500679016}],"concepts":[{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.7760999798774719},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.7161999940872192},{"id":"https://openalex.org/C24066741","wikidata":"https://www.wikidata.org/wiki/Q3796564","display_name":"Mass spectrometry imaging","level":3,"score":0.534500002861023},{"id":"https://openalex.org/C125965507","wikidata":"https://www.wikidata.org/wiki/Q1516595","display_name":"Laser capture microdissection","level":4,"score":0.4855000078678131},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.45210000872612},{"id":"https://openalex.org/C2779843651","wikidata":"https://www.wikidata.org/wiki/Q7390335","display_name":"SIGNAL (programming language)","level":2,"score":0.4242999851703644},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.414000004529953},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3831999897956848},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38199999928474426},{"id":"https://openalex.org/C80311884","wikidata":"https://www.wikidata.org/wiki/Q7268939","display_name":"Quantitative proteomics","level":4,"score":0.3709999918937683},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.3596999943256378},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3580999970436096},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.32499998807907104},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.31869998574256897},{"id":"https://openalex.org/C101092202","wikidata":"https://www.wikidata.org/wiki/Q6714252","display_name":"MALDI imaging","level":5,"score":0.3140000104904175},{"id":"https://openalex.org/C140779682","wikidata":"https://www.wikidata.org/wiki/Q210868","display_name":"Sampling (signal processing)","level":3,"score":0.3093999922275543},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.30390000343322754},{"id":"https://openalex.org/C2778576202","wikidata":"https://www.wikidata.org/wiki/Q2540164","display_name":"Sample preparation","level":2,"score":0.27869999408721924},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.27810001373291016},{"id":"https://openalex.org/C118530786","wikidata":"https://www.wikidata.org/wiki/Q1134732","display_name":"Instrumentation (computer programming)","level":2,"score":0.27090001106262207},{"id":"https://openalex.org/C136009344","wikidata":"https://www.wikidata.org/wiki/Q336201","display_name":"Confocal","level":2,"score":0.2705000042915344},{"id":"https://openalex.org/C15213710","wikidata":"https://www.wikidata.org/wiki/Q904207","display_name":"Ion-mobility spectrometry","level":3,"score":0.25940001010894775},{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.25679999589920044}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.07.749981","doi":"https://doi.org/10.64898/2026.09.07.749981","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.07.749981","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.07.749981.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.07.749981","doi":"https://doi.org/10.64898/2026.09.07.749981","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.07.749981","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.07.749981.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4332825680","display_name":null,"funder_award_id":"2022YFC3400800","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"}],"funders":[{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7212974107.pdf","grobid_xml":"https://content.openalex.org/works/W7212974107.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3119431472","https://openalex.org/W4415734943","https://openalex.org/W4405664391","https://openalex.org/W7168725084","https://openalex.org/W3047764503","https://openalex.org/W2594252827","https://openalex.org/W2246148395","https://openalex.org/W2317272249","https://openalex.org/W4226345305","https://openalex.org/W4393130839"],"abstract_inverted_index":{"Abstract":[0],"Integrating":[1],"spatial":[2,5,99,214],"metabolomics":[3],"and":[4,22,37,53,96,110,115,141,149,168,211,216],"proteomics":[6,27],"on":[7,31],"the":[8,60,104,173,176,184,191,198,207,220],"same":[9,61,221],"tissue":[10,62,67,131,180,222],"section":[11],"remains":[12],"challenging":[13],"because":[14],"matrix-assisted":[15],"laser":[16,23],"desorption/ionization":[17],"mass":[18],"spectrometry":[19,56],"imaging":[20],"(MALDI-MSI)":[21],"capture":[24],"microdissection":[25],"(LCM)-based":[26],"impose":[28],"different":[29,130,179],"requirements":[30],"sample":[32],"slides.":[33,128],"Here,":[34],"we":[35],"developed":[36],"systematically":[38],"evaluated":[39],"a":[40,69,202,227],"conductive":[41],"indium":[42],"tin":[43],"oxide-coated":[44],"polyethylene":[45],"terephthalate":[46],"(ITO-PET)":[47],"slide":[48,106,200],"that":[49],"enables":[50,212],"sequential":[51,213],"MALDI-MSI":[52,73,189],"LCM-liquid":[54],"chromatography-mass":[55],"(LCM-LC-MS)":[57],"analysis":[58,218],"of":[59,98,117,175],"section.":[63,223],"Using":[64],"mouse":[65,164],"brain":[66],"as":[68],"model,":[70],"ITO-PET":[71,105,199],"provided":[72],"performance":[74],"closely":[75],"comparable":[76,121,147],"to":[77,122],"conventional":[78,126],"ITO-glass,":[79],"including":[80],"spectral":[81],"concordance":[82],"(Pearson":[83],"correlation,":[84],"R":[85],"=":[86],"0.90),":[87],"ion":[88,139],"detection":[89],"coverage,":[90],"metabolite":[91],"annotation,":[92],"signal":[93,113,135],"intensity":[94,136],"distribution,":[95],"preservation":[97],"molecular":[100,234],"patterns.":[101],"Following":[102],"MALDI-MSI,":[103,151],"enabled":[107],"cutting-mode":[108],"LCM":[109,196],"yielded":[111],"proteomic":[112,134,217],"intensities":[114],"numbers":[116],"identified":[118,156],"protein":[119,142,157],"groups":[120],"those":[123],"obtained":[124],"with":[125,152,190],"PEN-glass":[127],"Across":[129],"sampling":[132],"areas,":[133],"distributions,":[137],"precursor":[138],"counts,":[140],"group":[143],"identifications":[144],"remained":[145],"broadly":[146],"before":[148],"after":[150],"substantial":[153],"overlap":[154],"in":[155,163],"groups.":[158],"Similar":[159],"patterns":[160],"were":[161],"observed":[162],"kidney,":[165],"lung,":[166],"spleen,":[167],"liver":[169],"tissues,":[170],"further":[171],"supporting":[172],"applicability":[174],"workflow":[177,225],"across":[178],"types.":[181],"By":[182],"combining":[183],"electrical":[185],"conductivity":[186],"required":[187,194],"for":[188,195,231],"mechanical":[192],"properties":[193],"cutting,":[197],"addresses":[201],"major":[203],"material":[204],"incompatibility":[205],"between":[206],"two":[208],"analytical":[209,229],"modalities":[210],"metabolomic":[215],"from":[219,236],"This":[224],"provides":[226],"practical":[228],"platform":[230],"obtaining":[232],"complementary":[233],"information":[235],"spatially":[237],"limited":[238],"biological":[239],"specimens.":[240]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-09-15T00:00:00"}