{"id":"https://openalex.org/W7212851669","doi":"https://doi.org/10.64898/2026.09.08.749775","title":"Site-resolved spatial and structural interactome of a human cell","display_name":"Site-resolved spatial and structural interactome of a human cell","publication_year":2026,"publication_date":"2026-09-14","ids":{"openalex":"https://openalex.org/W7212851669","doi":"https://doi.org/10.64898/2026.09.08.749775"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.08.749775","doi":"https://doi.org/10.64898/2026.09.08.749775","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.08.749775","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.08.749775.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.08.749775.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050324056","display_name":"Zehong Zhang","orcid":"https://orcid.org/0000-0003-1309-4395","observed_orcids":["https://orcid.org/0000-0003-1309-4395"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]},{"id":"https://openalex.org/I75951250","display_name":"Freie Universit\u00e4t Berlin","ror":"https://ror.org/046ak2485","country_code":"DE","type":"education","lineage":["https://openalex.org/I75951250"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Zehong Zhang","raw_affiliation_strings":["Department of Mathematics and Computer Science, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany","Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0003-1309-4395","affiliations":[{"raw_affiliation_string":"Department of Mathematics and Computer Science, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]},{"raw_affiliation_string":"Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065344089","display_name":"Nanako Yokoyama","orcid":"https://orcid.org/0009-0005-9968-8187","observed_orcids":["https://orcid.org/0009-0005-9968-8187"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Nanako Yokoyama","raw_affiliation_strings":["Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0009-0005-9968-8187","affiliations":[{"raw_affiliation_string":"Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101458224","display_name":"Ying Zhu","orcid":"https://orcid.org/0000-0003-4158-8093","observed_orcids":["https://orcid.org/0000-0003-4158-8093"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ying Zhu","raw_affiliation_strings":["Absea Biotechnology GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany","Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0003-4158-8093","affiliations":[{"raw_affiliation_string":"Absea Biotechnology GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":[]},{"raw_affiliation_string":"Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074886817","display_name":"Julia Ruta","orcid":"https://orcid.org/0009-0007-0524-6885","observed_orcids":["https://orcid.org/0009-0007-0524-6885"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Julia Ruta","raw_affiliation_strings":["Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0009-0007-0524-6885","affiliations":[{"raw_affiliation_string":"Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002409683","display_name":"Jiale Cheng","orcid":"https://orcid.org/0000-0002-0032-7455","observed_orcids":["https://orcid.org/0000-0002-0032-7455","https://orcid.org/0009-0008-0235-3420","https://orcid.org/0009-0008-2882-2934"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Jiale Cheng","raw_affiliation_strings":["Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0009-0008-0235-3420","affiliations":[{"raw_affiliation_string":"Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053804751","display_name":"Vini Natalia","orcid":"https://orcid.org/0009-0001-7266-6333","observed_orcids":["https://orcid.org/0009-0001-7266-6333"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Vini Natalia","raw_affiliation_strings":["Department of Molecular Physiology and Cell Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0009-0001-7266-6333","affiliations":[{"raw_affiliation_string":"Department of Molecular Physiology and Cell Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013452452","display_name":"Tolga Soykan","orcid":"https://orcid.org/0000-0002-1324-9601","observed_orcids":["https://orcid.org/0000-0002-1324-9601"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Tolga Soykan","raw_affiliation_strings":["Department of Molecular Physiology and Cell Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0002-1324-9601","affiliations":[{"raw_affiliation_string":"Department of Molecular Physiology and Cell Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010631390","display_name":"Volker Haucke","orcid":"https://orcid.org/0000-0003-3119-6993","observed_orcids":["https://orcid.org/0000-0003-3119-6993"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]},{"id":"https://openalex.org/I75951250","display_name":"Freie Universit\u00e4t Berlin","ror":"https://ror.org/046ak2485","country_code":"DE","type":"education","lineage":["https://openalex.org/I75951250"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Volker Haucke","raw_affiliation_strings":["Department of Biology, Chemistry, Pharmacy, Freie Universit\u00e4t Berlin, Berlin, Germany","Department of Molecular Physiology and Cell Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0003-3119-6993","affiliations":[{"raw_affiliation_string":"Department of Biology, Chemistry, Pharmacy, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]},{"raw_affiliation_string":"Department of Molecular Physiology and Cell Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100371030","display_name":"Fan Liu","orcid":"https://orcid.org/0000-0002-2358-549X","observed_orcids":["https://orcid.org/0000-0002-2358-549X"]},"institutions":[{"id":"https://openalex.org/I4210099563","display_name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie","ror":"https://ror.org/010s54n03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210099563"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Fan Liu","raw_affiliation_strings":["Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany","Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0002-2358-549X","affiliations":[{"raw_affiliation_string":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]},{"raw_affiliation_string":"Department of Structural Biology, Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany","institution_ids":["https://openalex.org/I4210099563"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5100371030"],"corresponding_institution_ids":["https://openalex.org/I4210099563","https://openalex.org/I7877124"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8169000148773193,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8169000148773193,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10887","display_name":"Bioinformatics and Genomic Networks","score":0.06199999898672104,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.05339999869465828,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.7940000295639038},{"id":"https://openalex.org/keywords/protein-complex-structure","display_name":"protein complex structure","score":0.48899999260902405},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.8080000281333923},{"id":"https://openalex.org/keywords/differential-ultracentrifugation","display_name":"differential ultracentrifugation","score":0.8209999799728394},{"id":"https://openalex.org/keywords/cross-linking-mass-spectrometry","display_name":"cross-linking mass spectrometry","score":0.9900000095367432},{"id":"https://openalex.org/keywords/short-linear-motifs","display_name":"short linear motifs","score":0.7429999709129333},{"id":"https://openalex.org/keywords/endomembrane-system","display_name":"endomembrane system","score":0.7710000276565552},{"id":"https://openalex.org/keywords/protein-trafficking","display_name":"protein trafficking","score":0.5419999957084656},{"id":"https://openalex.org/keywords/membrane-protein-orientation","display_name":"membrane protein orientation","score":0.8080000281333923}],"concepts":[{"id":"https://openalex.org/C9760119","wikidata":"https://www.wikidata.org/wiki/Q841382","display_name":"Interactome","level":3,"score":0.8907999992370605},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6955000162124634},{"id":"https://openalex.org/C94795543","wikidata":"https://www.wikidata.org/wiki/Q3813754","display_name":"Human proteome project","level":4,"score":0.6484000086784363},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.5982000231742859},{"id":"https://openalex.org/C2911029443","wikidata":"https://www.wikidata.org/wiki/Q21296145","display_name":"Human proteins","level":3,"score":0.5230000019073486},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.5160999894142151},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.5127000212669373},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47369998693466187},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4668000042438507},{"id":"https://openalex.org/C140051345","wikidata":"https://www.wikidata.org/wiki/Q7251501","display_name":"Protein subcellular localization prediction","level":3,"score":0.41200000047683716},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.38769999146461487},{"id":"https://openalex.org/C2993684226","wikidata":"https://www.wikidata.org/wiki/Q2662727","display_name":"Human cell","level":3,"score":0.3714999854564667},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.3269999921321869},{"id":"https://openalex.org/C152662350","wikidata":"https://www.wikidata.org/wiki/Q815297","display_name":"Systems biology","level":2,"score":0.313400000333786},{"id":"https://openalex.org/C197077220","wikidata":"https://www.wikidata.org/wiki/Q720988","display_name":"Human genome","level":4,"score":0.3100000023841858},{"id":"https://openalex.org/C145458877","wikidata":"https://www.wikidata.org/wiki/Q7317044","display_name":"Retromer","level":4,"score":0.30230000615119934},{"id":"https://openalex.org/C2994423619","wikidata":"https://www.wikidata.org/wiki/Q9103","display_name":"Human breast","level":4,"score":0.29760000109672546},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.28870001435279846},{"id":"https://openalex.org/C36464697","wikidata":"https://www.wikidata.org/wiki/Q451553","display_name":"Visualization","level":2,"score":0.28679999709129333},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.2847999930381775},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.27959999442100525},{"id":"https://openalex.org/C10010492","wikidata":"https://www.wikidata.org/wiki/Q3142557","display_name":"Protein sequencing","level":4,"score":0.27950000762939453},{"id":"https://openalex.org/C2994604686","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell function","level":3,"score":0.2775999903678894},{"id":"https://openalex.org/C159620131","wikidata":"https://www.wikidata.org/wiki/Q1938983","display_name":"Spatial analysis","level":2,"score":0.27140000462532043},{"id":"https://openalex.org/C27239569","wikidata":"https://www.wikidata.org/wiki/Q189029","display_name":"Cell fractionation","level":3,"score":0.2696000039577484},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.2685999870300293},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2630999982357025},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.26269999146461487},{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.2583000063896179},{"id":"https://openalex.org/C144292202","wikidata":"https://www.wikidata.org/wiki/Q898273","display_name":"Protein domain","level":3,"score":0.25290000438690186}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.08.749775","doi":"https://doi.org/10.64898/2026.09.08.749775","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.08.749775","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.08.749775.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.08.749775","doi":"https://doi.org/10.64898/2026.09.08.749775","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.08.749775","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/09/14/2026.09.08.749775.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3810819585","display_name":null,"funder_award_id":"HA2686/24-1","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G4099459689","display_name":null,"funder_award_id":"202304910020","funder_id":"https://openalex.org/F4320322725","funder_display_name":"China Scholarship Council"},{"id":"https://openalex.org/G8324706634","display_name":null,"funder_award_id":"P70/2018","funder_id":"https://openalex.org/F4320334763","funder_display_name":"Leibniz-Gemeinschaft"},{"id":"https://openalex.org/G8976983083","display_name":null,"funder_award_id":"HA2686","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"},{"id":"https://openalex.org/F4320322725","display_name":"China Scholarship Council","ror":"https://ror.org/04atp4p48"},{"id":"https://openalex.org/F4320334763","display_name":"Leibniz-Gemeinschaft","ror":"https://ror.org/01n6r0e97"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7212851669.pdf","grobid_xml":"https://content.openalex.org/works/W7212851669.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2613629789","https://openalex.org/W2594030753","https://openalex.org/W4312595699","https://openalex.org/W7150873569","https://openalex.org/W7167346695","https://openalex.org/W4321596020","https://openalex.org/W4393095886","https://openalex.org/W3010198187","https://openalex.org/W2618840381","https://openalex.org/W7214265601"],"abstract_inverted_index":{"Summary":[0],"The":[1,72],"spatial":[2,47,106,147],"and":[3,67,95,107,143,148],"structural":[4,108,149],"arrangement":[5,49],"of":[6,99,123,151],"proteins":[7],"determine":[8,61],"virtually":[9],"every":[10],"process":[11],"in":[12,41],"human":[13,152],"cells.":[14,43,153],"We":[15],"combined":[16],"gentle":[17],"subcellular":[18],"fractionation":[19],"by":[20,104],"differential":[21],"ultracentrifugation":[22],"with":[23,34,136],"cross-linking":[24],"mass":[25],"spectrometry":[26],"to":[27,54,89],"systematically":[28],"map":[29],"this":[30],"cellular":[31,65],"proteome":[32],"architecture":[33],"residue-level":[35,73],"evidence,":[36],"identifying":[37],"164,146":[38],"residue-to-residue":[39],"links":[40,45],"HEK293":[42,134],"These":[44],"capture":[46],"protein":[48,56,62,101],"at":[50,58],"a":[51,127],"resolution":[52],"sufficient":[53],"pinpoint":[55],"localizations":[57],"sub-organelle":[59],"level,":[60],"orientations":[63],"within":[64,114],"membranes,":[66],"identify":[68],"inter-organelle":[69],"contact":[70],"sites.":[71],"information":[74],"provides":[75],"evidence":[76],"for":[77],"18,074":[78],"direct":[79],"protein-protein":[80],"interactions":[81],"(PPIs),":[82],"which":[83],"we":[84,110,131],"integrate":[85],"into":[86],"AlphaFold-based":[87],"pipelines":[88],"nominate":[90],"PPI-mediating":[91],"short":[92],"linear":[93],"motifs":[94],"generate":[96],"assembly":[97],"models":[98],"large":[100],"complexes.":[102],"Guided":[103],"these":[105],"readouts,":[109],"discover":[111],"new":[112],"PPIs":[113],"the":[115,120,146],"endomembrane":[116],"system":[117],"that":[118],"regulate":[119],"compartmental":[121],"localization":[122],"trafficking":[124],"machinery.":[125],"Leveraging":[126],"network":[128],"topology-driven":[129],"strategy,":[130],"augment":[132],"our":[133],"dataset":[135],"PPI":[137],"data":[138],"from":[139],"different":[140],"cell":[141],"lines":[142],"methods,":[144],"expanding":[145],"interactome":[150]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2026-09-15T00:00:00"}