{"id":"https://openalex.org/W7212013944","doi":"https://doi.org/10.64898/2026.09.01.26361059","title":"One-pot CRISPR diagnostics for the rapid detection of Candida species and antimicrobial resistance biomarkers","display_name":"One-pot CRISPR diagnostics for the rapid detection of Candida species and antimicrobial resistance biomarkers","publication_year":2026,"publication_date":"2026-09-09","ids":{"openalex":"https://openalex.org/W7212013944","doi":"https://doi.org/10.64898/2026.09.01.26361059"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.01.26361059","doi":"https://doi.org/10.64898/2026.09.01.26361059","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.01.26361059","pdf_url":null,"source":{"id":"https://openalex.org/S3005729997","display_name":"medRxiv","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"medRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.09.01.26361059","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113056943","display_name":"Amy Heathcote","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Amy Heathcote","raw_affiliation_strings":["Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019825147","display_name":"Maya Bird","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Maya Bird","raw_affiliation_strings":["Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151082373","display_name":"Hyeri Jeong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Hyeri Jeong","raw_affiliation_strings":["Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5150935469","display_name":"Xena Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Xena Li","raw_affiliation_strings":["Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada","Shared Hospital Laboratories, Toronto, ON, M4N 3M5, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Shared Hospital Laboratories, Toronto, ON, M4N 3M5, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5140572988","display_name":"Robert Kozak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]},{"id":"https://openalex.org/I4391768120","display_name":"Sunnybrook Research Institute","ror":"https://ror.org/05n0tzs53","country_code":"CA","type":"facility","lineage":["https://openalex.org/I1323843004","https://openalex.org/I185261750","https://openalex.org/I4391768120"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Robert Kozak","raw_affiliation_strings":["Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada","Shared Hospital Laboratories, Toronto, ON, M4N 3M5, Canada","Sunnybrook Research Institute, Toronto, ON, M4N 3M5, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Shared Hospital Laboratories, Toronto, ON, M4N 3M5, Canada","institution_ids":[]},{"raw_affiliation_string":"Sunnybrook Research Institute, Toronto, ON, M4N 3M5, Canada","institution_ids":["https://openalex.org/I4391768120"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055266752","display_name":"Nicole E. Weckman","orcid":"https://orcid.org/0000-0001-5894-4926","observed_orcids":["https://orcid.org/0000-0001-5894-4926"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Nicole E. Weckman","raw_affiliation_strings":["Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada","Institute for Studies in Transdisciplinary Engineering Education & Practice, University of Toronto, Toronto, ON M5S 1A1, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 1A1, Canada","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Institute for Studies in Transdisciplinary Engineering Education & Practice, University of Toronto, Toronto, ON M5S 1A1, Canada","institution_ids":["https://openalex.org/I185261750"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.3564000129699707,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.3564000129699707,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.30880001187324524,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10150","display_name":"Antifungal resistance and susceptibility","score":0.225600004196167,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-based-diagnostics","display_name":"CRISPR-based diagnostics","score":0.7730000019073486},{"id":"https://openalex.org/keywords/candida-species-identification","display_name":"Candida species identification","score":0.7549999952316284},{"id":"https://openalex.org/keywords/antimicrobial-resistance","display_name":"antimicrobial resistance","score":0.7850000262260437},{"id":"https://openalex.org/keywords/azole-resistance","display_name":"azole resistance","score":0.6639999747276306},{"id":"https://openalex.org/keywords/echinocandin-resistance","display_name":"echinocandin resistance","score":0.9990000128746033},{"id":"https://openalex.org/keywords/single-nucleotide-polymorphisms","display_name":"single nucleotide polymorphisms","score":0.8690000176429749},{"id":"https://openalex.org/keywords/fungal-infection","display_name":"fungal infection","score":0.6940000057220459},{"id":"https://openalex.org/keywords/rapid-pathogen-detection","display_name":"rapid pathogen detection","score":0.875}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9143999814987183},{"id":"https://openalex.org/C2779320740","wikidata":"https://www.wikidata.org/wiki/Q3718510","display_name":"Echinocandin","level":4,"score":0.6043999791145325},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5580999851226807},{"id":"https://openalex.org/C2779548794","wikidata":"https://www.wikidata.org/wiki/Q578726","display_name":"Antifungal","level":2,"score":0.4925000071525574},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.424699991941452},{"id":"https://openalex.org/C2909938017","wikidata":"https://www.wikidata.org/wiki/Q2712836","display_name":"Candida infections","level":3,"score":0.4099000096321106},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3873000144958496},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.38260000944137573},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.3815000057220459},{"id":"https://openalex.org/C94665300","wikidata":"https://www.wikidata.org/wiki/Q63391344","display_name":"Antibiotic resistance","level":3,"score":0.36649999022483826},{"id":"https://openalex.org/C116675565","wikidata":"https://www.wikidata.org/wiki/Q3241045","display_name":"Outbreak","level":2,"score":0.36579999327659607},{"id":"https://openalex.org/C114851261","wikidata":"https://www.wikidata.org/wiki/Q12147416","display_name":"Drug resistance","level":2,"score":0.3424000144004822},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.3407000005245209},{"id":"https://openalex.org/C2776315533","wikidata":"https://www.wikidata.org/wiki/Q3241189","display_name":"Acinetobacter baumannii","level":4,"score":0.3059000074863434},{"id":"https://openalex.org/C82157600","wikidata":"https://www.wikidata.org/wiki/Q2671652","display_name":"Diagnostic test","level":2,"score":0.30149999260902405},{"id":"https://openalex.org/C2909919671","wikidata":"https://www.wikidata.org/wiki/Q578726","display_name":"Antifungal drugs","level":3,"score":0.29980000853538513},{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.275299996137619},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.27090001106262207},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2687000036239624},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.2678000032901764},{"id":"https://openalex.org/C2780917455","wikidata":"https://www.wikidata.org/wiki/Q310443","display_name":"Candida albicans","level":2,"score":0.2623000144958496}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.01.26361059","doi":"https://doi.org/10.64898/2026.09.01.26361059","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.01.26361059","pdf_url":null,"source":{"id":"https://openalex.org/S3005729997","display_name":"medRxiv","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"medRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.01.26361059","doi":"https://doi.org/10.64898/2026.09.01.26361059","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.01.26361059","pdf_url":null,"source":{"id":"https://openalex.org/S3005729997","display_name":"medRxiv","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"medRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":60,"referenced_works":["https://openalex.org/W1964521129","https://openalex.org/W2005936443","https://openalex.org/W2026308901","https://openalex.org/W2064931183","https://openalex.org/W2111310284","https://openalex.org/W2131829756","https://openalex.org/W2133029063","https://openalex.org/W2165869860","https://openalex.org/W2167903799","https://openalex.org/W2231020609","https://openalex.org/W2341755703","https://openalex.org/W2410526873","https://openalex.org/W2606915822","https://openalex.org/W2607086226","https://openalex.org/W2742894922","https://openalex.org/W2750959598","https://openalex.org/W2766989075","https://openalex.org/W2785904295","https://openalex.org/W2787024379","https://openalex.org/W2790715348","https://openalex.org/W2804198042","https://openalex.org/W2810059222","https://openalex.org/W2892949163","https://openalex.org/W2903321797","https://openalex.org/W2918567627","https://openalex.org/W2932341513","https://openalex.org/W2939661038","https://openalex.org/W2950877338","https://openalex.org/W2951622756","https://openalex.org/W2973445868","https://openalex.org/W2982133187","https://openalex.org/W2990437752","https://openalex.org/W3012033772","https://openalex.org/W3020077371","https://openalex.org/W3026958586","https://openalex.org/W3034483708","https://openalex.org/W3087209706","https://openalex.org/W3087541931","https://openalex.org/W3125504512","https://openalex.org/W3129956322","https://openalex.org/W3156847645","https://openalex.org/W3178418858","https://openalex.org/W3189188210","https://openalex.org/W4220710122","https://openalex.org/W4307840375","https://openalex.org/W4310184775","https://openalex.org/W4313907616","https://openalex.org/W4318676878","https://openalex.org/W4327744893","https://openalex.org/W4381250317","https://openalex.org/W4386440508","https://openalex.org/W4395049051","https://openalex.org/W4400800973","https://openalex.org/W4404071348","https://openalex.org/W4404093341","https://openalex.org/W4406961073","https://openalex.org/W4407039602","https://openalex.org/W4408621983","https://openalex.org/W4408877609","https://openalex.org/W7123399719"],"related_works":["https://openalex.org/W7132888356","https://openalex.org/W4412637366","https://openalex.org/W7123399719","https://openalex.org/W7151891935","https://openalex.org/W4405656600","https://openalex.org/W7171764956","https://openalex.org/W3000117160","https://openalex.org/W4414419194","https://openalex.org/W3114057961","https://openalex.org/W4413448570"],"abstract_inverted_index":{"ABSTRACT":[0],"Antimicrobial":[1],"resistance":[2,56,104],"(AMR)":[3],"is":[4],"a":[5,46,131],"growing":[6],"public":[7],"health":[8],"threat,":[9],"where":[10],"drug-resistant":[11],"Candida":[12,52,71],"infections":[13],"are":[14,30,65,86],"associated":[15,106],"with":[16,76,107],"increased":[17],"hospital":[18],"costs,":[19],"lengths":[20],"of":[21,99,117,120,127,145],"stay,":[22],"and":[23,32,38,54,79,109,142],"high":[24],"mortality":[25],"rates.":[26],"Current":[27],"diagnostic":[28,49],"methods":[29],"slow":[31],"labor-intensive,":[33],"causing":[34],"delays":[35],"in":[36,58],"diagnosis":[37],"patient":[39],"treatment.":[40],"In":[41],"this":[42],"research,":[43],"we":[44],"propose":[45],"rapid":[47],"CRISPR-based":[48],"for":[50,67,124,133],"identifying":[51],"pathogens":[53],"antifungal":[55],"biomarkers":[57],"clinical":[59],"applications.":[60],"One-pot":[61],"CRISPR":[62,90,122],"assay":[63],"designs":[64],"optimized":[66],"three":[68],"clinically":[69],"relevant":[70],"species,":[72],"achieving":[73],"attomolar":[74],"sensitivity":[75],"synthetic":[77],"DNA":[78],"validating":[80],"performance":[81],"on":[82],"cultured":[83],"isolates.":[84],"Strategies":[85],"developed":[87],"to":[88,92,139],"engineer":[89],"gRNA":[91],"control":[93],"one-pot":[94,121],"reaction":[95],"kinetics,":[96],"enhancing":[97],"discrimination":[98],"single":[100],"nucleotide":[101],"polymorphism":[102],"(SNP)":[103],"mutations":[105],"azole":[108],"echinocandin":[110],"antifungals.":[111],"This":[112],"research":[113],"expands":[114],"our":[115],"understanding":[116],"the":[118],"design":[119],"reactions":[123],"sensitive":[125],"detection":[126],"DNA,":[128],"while":[129],"establishing":[130],"foundation":[132],"faster,":[134],"more":[135],"precise":[136],"AMR":[137],"diagnostics":[138],"improve":[140],"surveillance":[141],"outbreak":[143],"management":[144],"fungal":[146],"infections.":[147]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-10T00:00:00"}
